Experiment set4IT032 for Escherichia coli BW25113

Compare to:

LB with 3-methyl-3-butenol 0.31 vol%

200 most important genes:

  gene name fitness t score description  
b0630 lipB -3.1 -3.6 protein of lipoate biosynthesis (VIMSS) compare
b4375 prfC -2.9 -10.4 peptide chain release factor 3 (NCBI) compare
b2699 recA -2.7 -4.1 recombinase A (NCBI) compare
b2330 yfcB -2.5 -4.4 putative adenine-specific methylase (VIMSS) compare
b0118 acnB -2.5 -4.9 aconitate hydratase (NCBI) compare
b2620 smpB -2.4 -3.0 SsrA-binding protein (NCBI) compare
b3725 pstB -2.3 -4.4 phosphate transporter subunit (NCBI) compare
b3619 rfaD -2.3 -5.7 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2935 tktA -2.3 -4.8 transketolase 1, thiamin-binding (NCBI) compare
b2567 rnc -2.2 -2.9 ribonuclease III (NCBI) compare
b3162 deaD -2.2 -8.0 inducible ATP-independent RNA helicase (VIMSS) compare
b0200 gmhB -2.1 -2.8 hypothetical protein (NCBI) compare
b2528 iscA -2.1 -3.2 iron-sulfur cluster assembly protein (NCBI) compare
b3390 aroK -2.0 -3.3 shikimate kinase I (NCBI) compare
b3621 rfaC -2.0 -7.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2688 gshA -2.0 -9.2 glutamate--cysteine ligase (NCBI) compare
b0726 sucA -1.8 -5.2 alpha-ketoglutarate decarboxylase (NCBI) compare
b3052 rfaE -1.8 -6.6 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1291 sapD -1.8 -3.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1192 ldcA -1.7 -6.5 L,D-carboxypeptidase A (NCBI) compare
b2287 nuoB -1.7 -5.5 NADH dehydrogenase subunit B (NCBI) compare
b2822 recC -1.7 -6.4 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b0149 mrcB -1.7 -11.7 penicillin-binding protein 1b (NCBI) compare
b0439 lon -1.7 -6.4 DNA-binding ATP-dependent protease La (NCBI) compare
b0053 surA -1.7 -6.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b4239 treC -1.6 -6.8 trehalose-6-P hydrolase (NCBI) compare
b4177 purA -1.6 -5.1 adenylosuccinate synthetase (NCBI) compare
b0222 lpcA -1.6 -3.6 phosphoheptose isomerase (NCBI) compare
b2820 recB -1.6 -3.6 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3807 cyaY -1.6 -5.9 frataxin-like protein (NCBI) compare
b0683 fur -1.6 -2.6 ferric uptake regulator (NCBI) compare
b2948 yqgE -1.6 -3.9 orf, hypothetical protein (VIMSS) compare
b3609 secB -1.6 -3.5 export protein SecB (NCBI) compare
b0890 ftsK -1.5 -8.5 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2277 nuoM -1.5 -7.3 NADH dehydrogenase subunit M (NCBI) compare
b0723 sdhA -1.5 -9.8 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b1863 ruvC -1.5 -4.4 Holliday junction resolvase (NCBI) compare
b3463 ftsE -1.5 -5.2 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0721 sdhC -1.5 -5.2 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b3290 trkA -1.5 -4.2 potassium transporter peripheral membrane component (NCBI) compare
b2947 gshB -1.5 -5.6 glutathione synthetase (NCBI) compare
b3871 bipA -1.5 -9.8 GTP-binding protein (NCBI) compare
b0722 sdhD -1.5 -4.6 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b2279 nuoK -1.5 -2.8 NADH dehydrogenase subunit K (NCBI) compare
b0720 gltA -1.4 -5.5 citrate synthase (NCBI) compare
b2576 srmB -1.4 -4.4 ATP-dependent RNA helicase (NCBI) compare
b1292 sapC -1.4 -3.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3493 pitA -1.4 -8.6 phosphate transporter, low-affinity (NCBI) compare
b1850 eda -1.4 -1.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b4172 hfq -1.4 -2.5 RNA-binding protein Hfq (NCBI) compare
b3728 pstS -1.4 -5.3 phosphate transporter subunit (NCBI) compare
b0356 frmA -1.3 -6.8 alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI) conserved
b0428 cyoE -1.3 -5.9 protoheme IX farnesyltransferase (NCBI) compare
b3462 ftsX -1.3 -6.1 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2280 nuoJ -1.3 -5.9 NADH dehydrogenase subunit J (NCBI) compare
b2297 pta -1.3 -2.3 phosphate acetyltransferase (NCBI) compare
b2276 nuoN -1.3 -6.2 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3778 rep -1.3 -8.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2278 nuoL -1.3 -8.1 NADH dehydrogenase subunit L (NCBI) compare
b2281 nuoI -1.3 -3.2 NADH dehydrogenase subunit I (NCBI) compare
b0729 sucD -1.3 -3.5 succinyl-CoA synthetase subunit alpha (NCBI) compare
b0114 aceE -1.2 -2.9 pyruvate dehydrogenase subunit E1 (NCBI) compare
b1105 ycfM -1.2 -4.0 predicted outer membrane lipoprotein (NCBI) compare
b2283 nuoG -1.2 -7.2 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b1693 aroD -1.2 -5.8 3-dehydroquinate dehydratase (NCBI) compare
b3426 glpD -1.2 -6.4 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b1236 galU -1.2 -5.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0767 ybhE -1.2 -4.7 6-phosphogluconolactonase (NCBI) compare
b3164 pnp -1.2 -3.3 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4155 yjeA -1.2 -1.8 putative lysyl-tRNA synthetase (VIMSS) compare
b3706 trmE -1.2 -6.0 tRNA modification GTPase (NCBI) compare
b1290 sapF -1.2 -2.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3169 nusA -1.2 -2.8 transcription elongation factor NusA (NCBI) compare
b3870 glnA -1.2 -3.1 glutamine synthetase (NCBI) compare
b0724 sdhB -1.1 -4.7 succinate dehydrogenase, FeS subunit (NCBI) compare
b3389 aroB -1.1 -8.3 3-dehydroquinate synthase (NCBI) compare
b3806 cyaA -1.1 -5.3 adenylate cyclase (NCBI) compare
b3414 gntY -1.1 -5.4 predicted gluconate transport associated protein (NCBI) compare
b2288 nuoA -1.1 -3.1 NADH dehydrogenase I chain A (VIMSS) compare
b0082 mraW -1.1 -3.8 S-adenosyl-methyltransferase (NCBI) compare
b3341 rpsG -1.1 -1.4 30S ribosomal protein S7 (NCBI) compare
b2830 nudH -1.1 -1.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b3631 rfaG -1.1 -9.2 glucosyltransferase I (NCBI) compare
b2282 nuoH -1.1 -3.2 NADH dehydrogenase subunit H (NCBI) compare
b3281 aroE -1.1 -8.8 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3839 tatC -1.1 -6.9 TatABCE protein translocation system subunit (NCBI) compare
b3146 yraL -1.0 -4.9 predicted methyltransferase (NCBI) compare
b2051 wcaH -1.0 -1.4 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2894 xerD -1.0 -1.7 site-specific tyrosine recombinase XerD (NCBI) compare
b2286 nuoC -1.0 -4.9 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3630 rfaP -1.0 -7.4 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3620 rfaF -1.0 -4.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3026 qseC -1.0 -4.6 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2892 recJ -1.0 -5.5 ssDNA exonuclease, 5' --> 3'-specific (NCBI) compare
b3741 gidA -1.0 -5.9 glucose-inhibited division protein A (NCBI) compare
b3727 pstC -1.0 -5.4 phosphate transporter subunit (NCBI) compare
b3386 rpe -0.9 -1.4 ribulose-phosphate 3-epimerase (NCBI) compare
b4648 ysaC -0.9 -2.8 no description compare
b1426 ydcH -0.9 -1.8 orf, hypothetical protein (VIMSS) compare
b2284 nuoF -0.9 -3.9 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b0741 pal -0.9 -3.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2318 truA -0.9 -3.5 tRNA pseudouridine synthase A (NCBI) compare
b1476 fdnI -0.9 -1.8 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2340 sixA -0.9 -3.9 phosphohistidine phosphatase (NCBI) compare
b3198 kdsC -0.9 -2.5 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b4685 yrbN -0.9 -1.6 expressed protein (RefSeq) compare
b0429 cyoD -0.9 -3.8 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b0238 gpt -0.9 -3.5 xanthine phosphoribosyltransferase (NCBI) compare
b3844 fre -0.9 -4.4 NAD(P)H-flavin reductase (NCBI) compare
b3811 xerC -0.9 -2.6 site-specific tyrosine recombinase XerC (NCBI) compare
b3035 tolC -0.9 -5.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4438 cyaR -0.9 -2.4 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b1293 sapB -0.9 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3962 udhA -0.9 -6.3 putative oxidoreductase (VIMSS) compare
b2104 thiM -0.9 -2.6 hydroxyethylthiazole kinase (NCBI) compare
b3842 rfaH -0.9 -4.0 transcriptional activator RfaH (NCBI) compare
b0888 trxB -0.9 -2.7 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1861 ruvA -0.9 -1.0 Holliday junction DNA helicase motor protein (NCBI) compare
b4234 yjgA -0.9 -3.6 hypothetical protein (NCBI) compare
b0431 cyoB -0.9 -6.3 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b3183 obgE -0.9 -1.4 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0740 tolB -0.9 -3.9 translocation protein TolB precursor (NCBI) compare
b0432 cyoA -0.8 -5.4 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b0738 tolR -0.8 -2.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1084 rne -0.8 -2.5 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b0677 nagA -0.8 -4.6 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b2263 yfbB -0.8 -2.7 predicted peptidase (NCBI) compare
b3204 ptsN -0.8 -3.5 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2569 lepA -0.8 -3.3 GTP-binding protein LepA (NCBI) compare
b0049 apaH -0.8 -3.1 diadenosinetetraphosphatase (NCBI) compare
b0728 sucC -0.8 -4.1 succinyl-CoA synthetase subunit beta (NCBI) compare
b3726 pstA -0.8 -3.8 phosphate transporter subunit (NCBI) compare
b0145 dksA -0.8 -2.8 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3649 rpoZ -0.8 -2.5 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3261 fis -0.8 -2.3 DNA-binding protein Fis (NCBI) compare
b3636 rpmG -0.8 -1.7 50S ribosomal protein L33 (NCBI) compare
b2456 cchB -0.8 -3.4 predicted carboxysome structural protein, ethanolamine utilization protein (NCBI) compare
b3977 tyrU -0.8 -0.9 tRNA-Tyr (NCBI) compare
b3237 argR -0.8 -3.6 arginine repressor (NCBI) compare
b1162 ycgE -0.8 -3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3979 thrT -0.8 -1.0 tRNA-Thr (NCBI) compare
b1675 ydhZ -0.8 -2.5 hypothetical protein (NCBI) compare
b1361 ydaW -0.8 -1.0 orf, hypothetical protein (VIMSS) compare
b2501 ppk -0.8 -5.4 polyphosphate kinase (NCBI) compare
b3761 trpT -0.8 -1.5 tRNA-Trp (NCBI) compare
b0928 aspC -0.7 -4.2 aspartate aminotransferase, PLP-dependent (NCBI) compare
b4505 insX -0.7 -1.6 predicted IS protein (RefSeq) compare
b2512 yfgL -0.7 -3.2 protein assembly complex, lipoprotein component (NCBI) compare
b3961 oxyR -0.7 -4.9 DNA-binding transcriptional dual regulator (NCBI) compare
b3652 recG -0.7 -5.2 ATP-dependent DNA helicase (NCBI) compare
b0437 clpP -0.7 -4.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2285 nuoE -0.7 -1.5 NADH dehydrogenase subunit E (NCBI) compare
b1811 yoaH -0.7 -2.7 hypothetical protein (NCBI) compare
b1658 purR -0.7 -3.5 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1090 plsX -0.7 -3.3 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b0564 appY -0.7 -1.1 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0430 cyoC -0.7 -2.6 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b1279 yciS -0.7 -0.8 conserved inner membrane protein (NCBI) compare
b0782 moaB -0.7 -1.5 molybdopterin biosynthesis protein B (NCBI) compare
b3863 polA -0.7 -1.0 DNA polymerase I (NCBI) compare
b0862 artQ -0.7 -2.1 arginine transporter subunit (NCBI) compare
b0438 clpX -0.7 -4.1 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0051 ksgA -0.7 -3.4 dimethyladenosine transferase (NCBI) compare
b1836 yebV -0.7 -2.4 orf, hypothetical protein (VIMSS) compare
b1627 rsxA -0.7 -2.7 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b0481 ybaK -0.7 -2.4 hypothetical protein (NCBI) compare
b1138 ymfE -0.7 -1.1 e14 prophage; predicted inner membrane protein (NCBI) compare
b4202 rpsR -0.7 -1.1 30S ribosomal protein S18 (NCBI) compare
b2618 b2618 -0.7 -2.5 orf, hypothetical protein (VIMSS) compare
b0843 ybjH -0.7 -1.8 hypothetical protein (NCBI) compare
b0623 cspE -0.7 -1.7 cold shock protein E (NCBI) compare
b1095 fabF -0.7 -3.9 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3236 mdh -0.7 -3.9 malate dehydrogenase (NCBI) compare
b0688 pgm -0.6 -2.5 phosphoglucomutase (NCBI) compare
b1125 potB -0.6 -2.8 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3792 wzxE -0.6 -4.4 O-antigen translocase (NCBI) compare
b2513 yfgM -0.6 -2.4 hypothetical protein (NCBI) compare
b3465 yhhF -0.6 -3.5 predicted methyltransferase (NCBI) compare
b2025 hisF -0.6 -2.3 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b1109 ndh -0.6 -2.8 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b0920 ycbC -0.6 -2.9 conserved inner membrane protein (NCBI) compare
b2808 gcvA -0.6 -3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1263 trpD -0.6 -1.9 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b2564 pdxJ -0.6 -2.5 pyridoxal phosphate biosynthetic protein (NCBI) compare
b3868 glnG -0.6 -3.3 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b2937 speB -0.6 -3.8 agmatinase (NCBI) compare
b3136 agaS -0.6 -2.7 tagatose-6-phosphate ketose/aldose isomerase (NCBI) compare
b4180 rlmB -0.6 -3.8 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b1124 potC -0.6 -2.8 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b4042 dgkA -0.6 -2.1 diacylglycerol kinase (NCBI) compare
b3617 kbl -0.6 -3.7 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b1679 sufE -0.6 -2.5 cysteine desufuration protein SufE (NCBI) compare
b3229 sspA -0.6 -1.9 stringent starvation protein A (NCBI) compare
b0949 uup -0.6 -3.6 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1213 ychQ -0.6 -1.7 predicted transcriptional regulator (NCBI) compare
b2895 fldB -0.6 -2.0 flavodoxin 2 (NCBI) compare
b1858 znuC -0.6 -2.5 high-affinity zinc transporter ATPase (NCBI) compare
b1855 lpxM -0.6 -2.3 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3658 selC -0.6 -1.6 tRNA-Sec (NCBI) compare
b4139 aspA -0.6 -3.9 aspartate ammonia-lyase (aspartase) (VIMSS) compare


Specific Phenotypes

For 2 genes in this experiment

For stress 3-methyl-3-butenol in Escherichia coli BW25113

For stress 3-methyl-3-butenol across organisms