Experiment set4IT027 for Pseudomonas fluorescens GW456-L13

Compare to:

KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x

200 most important genes:

  gene name fitness t score description  
PfGW456L13_2192 -4.9 -4.7 regulator of length of O-antigen component of lipopolysaccharide chains compare
PfGW456L13_1337 -4.4 -6.0 DedA protein compare
PfGW456L13_3995 -4.2 -6.9 UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14) compare
PfGW456L13_1584 -3.9 -2.7 Molybdopterin biosynthesis protein MoeB compare
PfGW456L13_1479 -3.7 -1.6 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
PfGW456L13_1520 -3.7 -2.5 Pantoate--beta-alanine ligase (EC 6.3.2.1) compare
PfGW456L13_3996 -3.1 -6.5 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
PfGW456L13_875 -3.1 -6.0 Exopolyphosphatase (EC 3.6.1.11) compare
PfGW456L13_1010 -2.9 -6.8 Pyrroline-5-carboxylate reductase (EC 1.5.1.2) compare
PfGW456L13_1000 -2.9 -7.2 Thiazole biosynthesis protein ThiG compare
PfGW456L13_3999 -2.8 -1.9 N-acetylmannosaminyltransferase (EC 2.4.1.187) compare
PfGW456L13_2955 -2.8 -3.1 RND efflux system, membrane fusion protein CmeA compare
PfGW456L13_1384 -2.6 -2.4 Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) compare
PfGW456L13_1863 -2.4 -3.1 Quinolinate synthetase (EC 2.5.1.72) compare
PfGW456L13_1198 -2.4 -4.9 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
PfGW456L13_2885 -2.3 -5.1 BarA-associated response regulator UvrY (= GacA = SirA) compare
PfGW456L13_5011 -2.2 -3.0 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
PfGW456L13_2250 -2.2 -5.2 Probable protease htpX homolog (EC 3.4.24.-) compare
PfGW456L13_1836 -2.1 -4.3 Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein compare
PfGW456L13_275 -2.1 -1.4 3-dehydroquinate synthase (EC 4.2.3.4) compare
PfGW456L13_4026 -2.1 -1.3 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
PfGW456L13_276 -2.1 -1.2 Shikimate kinase I (EC 2.7.1.71) compare
PfGW456L13_1099 -2.0 -2.3 FIG00953563: hypothetical protein compare
PfGW456L13_4511 -2.0 -1.1 Rhodanese-like domain protein compare
PfGW456L13_2594 -2.0 -2.6 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
PfGW456L13_1385 -1.9 -3.9 Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7) compare
PfGW456L13_793 -1.9 -1.3 Acetylglutamate kinase (EC 2.7.2.8) compare
PfGW456L13_4517 -1.9 -9.4 L-aspartate oxidase (EC 1.4.3.16) compare
PfGW456L13_2924 -1.8 -5.5 Glutathione reductase (EC 1.8.1.7) compare
PfGW456L13_5082 -1.8 -1.2 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
PfGW456L13_1377 -1.8 -4.5 Magnesium and cobalt efflux protein CorC compare
PfGW456L13_644 -1.8 -5.7 Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25) compare
PfGW456L13_468 -1.7 -2.3 FIG00953394: hypothetical protein compare
PfGW456L13_1889 -1.7 -2.3 Phosphogluconate dehydratase (EC 4.2.1.12) compare
PfGW456L13_5096 -1.7 -1.5 FIG004454: RNA binding protein compare
PfGW456L13_2029 -1.7 -9.0 BarA sensory histidine kinase (= VarS = GacS) compare
PfGW456L13_187 -1.6 -10.0 Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17) compare
PfGW456L13_1853 -1.6 -2.4 Holliday junction DNA helicase RuvB compare
PfGW456L13_5003 -1.6 -1.7 YrbA protein compare
PfGW456L13_5067 -1.6 -2.5 Ornithine decarboxylase (EC 4.1.1.17) / Arginine decarboxylase (EC 4.1.1.19) compare
PfGW456L13_4580 -1.6 -5.2 Multidrug efflux RND membrane fusion protein MexC compare
PfGW456L13_1204 -1.6 -7.4 COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases compare
PfGW456L13_4961 -1.6 -5.2 Fumarylacetoacetase (EC 3.7.1.2) compare
PfGW456L13_4645 -1.6 -10.1 AmpG permease compare
PfGW456L13_2954 -1.5 -6.4 RND efflux system, inner membrane transporter CmeB compare
PfGW456L13_50 -1.5 -5.6 3-dehydroquinate dehydratase II (EC 4.2.1.10) compare
PfGW456L13_310 -1.5 -9.6 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
PfGW456L13_4253 -1.5 -2.0 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
PfGW456L13_351 -1.5 -3.4 Glutaredoxin 3 (Grx3) compare
PfGW456L13_3997 -1.5 -3.5 hypothetical protein compare
PfGW456L13_3409 -1.4 -3.0 Transcriptional regulator, IclR family compare
PfGW456L13_751 -1.4 -2.0 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
PfGW456L13_2034 -1.4 -2.0 hypothetical protein compare
PfGW456L13_2956 -1.4 -3.6 Transcriptional regulatory protein RstA compare
PfGW456L13_1235 -1.4 -4.9 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-) compare
PfGW456L13_2551 -1.4 -1.8 Cell division trigger factor (EC 5.2.1.8) compare
PfGW456L13_4254 -1.3 -3.5 FIG00955360: hypothetical protein compare
PfGW456L13_872 -1.3 -6.1 FIG139438: lipoprotein B compare
PfGW456L13_994 -1.3 -2.1 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
PfGW456L13_3598 -1.3 -1.9 hypothetical protein compare
PfGW456L13_2957 -1.3 -2.7 Sensory histidine kinase QseC compare
PfGW456L13_1391 -1.3 -0.9 Transcriptional regulator, AsnC family compare
PfGW456L13_44 -1.3 -3.1 Translation initiation factor SUI1-related protein compare
PfGW456L13_1236 -1.3 -6.4 Cell wall endopeptidase, family M23/M37 compare
PfGW456L13_4041 -1.3 -1.8 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) compare
PfGW456L13_913 -1.2 -2.5 N-acetylglutamate synthase (EC 2.3.1.1) compare
PfGW456L13_4094 -1.2 -4.6 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-) compare
PfGW456L13_4863 -1.2 -2.0 FIG021952: putative membrane protein compare
PfGW456L13_3994 -1.2 -6.3 Tyrosine-protein kinase Wzc (EC 2.7.10.2) compare
PfGW456L13_1205 -1.2 -4.0 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
PfGW456L13_1637 -1.2 -3.4 Stringent starvation protein A compare
PfGW456L13_1861 -1.2 -2.4 Queuosine Biosynthesis QueE Radical SAM compare
PfGW456L13_1233 -1.2 -0.8 N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38) compare
PfGW456L13_1160 -1.2 -3.2 Putative DNA-binding protein in cluster with Type I restriction-modification system compare
PfGW456L13_766 -1.2 -1.7 hypothetical protein compare
PfGW456L13_1021 -1.2 -1.8 Glutathione synthetase (EC 6.3.2.3) compare
PfGW456L13_311 -1.1 -6.2 Glucans biosynthesis protein G precursor compare
PfGW456L13_2649 -1.1 -2.4 Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6) compare
PfGW456L13_3333 -1.1 -2.8 hypothetical protein compare
PfGW456L13_1973 -1.1 -1.8 Arginine N-succinyltransferase (EC 2.3.1.109) compare
PfGW456L13_757 -1.1 -2.3 Phosphate transport system regulatory protein PhoU compare
PfGW456L13_2333 -1.1 -3.8 Glycosyltransferase (EC 2.4.1.-) compare
PfGW456L13_760 -1.1 -4.4 Phosphate regulon metal ion transporter containing CBS domains compare
PfGW456L13_2097 -1.1 -2.8 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) compare
PfGW456L13_931 -1.1 -5.0 Putrescine transport system permease protein PotH (TC 3.A.1.11.2) compare
PfGW456L13_4233 -1.1 -2.2 Transcriptional regulator, MarR family compare
PfGW456L13_2424 -1.1 -1.4 Phenylacetate-CoA oxygenase, PaaJ subunit compare
PfGW456L13_420 -1.1 -1.2 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
PfGW456L13_1472 -1.1 -3.4 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
PfGW456L13_1705 -1.1 -2.2 FIG00955494: hypothetical protein compare
PfGW456L13_4067 -1.1 -3.1 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
PfGW456L13_2185 -1.1 -1.7 putative colanic acid biosynthesis glycosyl transferase compare
PfGW456L13_2170 -1.0 -1.7 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
PfGW456L13_812 -1.0 -1.6 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
PfGW456L13_2251 -1.0 -6.1 hypothetical protein compare
PfGW456L13_1845 -1.0 -0.7 Cold shock protein CspC compare
PfGW456L13_354 -1.0 -5.1 Periplasmic septal ring factor with murein hydrolase activity EnvC/YibP compare
PfGW456L13_859 -1.0 -2.4 Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives) compare
PfGW456L13_1984 -1.0 -1.4 Mg/Co/Ni transporter MgtE / CBS domain compare
PfGW456L13_1003 -1.0 -4.1 Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB compare
PfGW456L13_3354 -1.0 -2.2 hypothetical protein compare
PfGW456L13_4662 -1.0 -1.4 Transcriptional regulator, LuxR family compare
PfGW456L13_2853 -1.0 -1.3 FIG00955561: hypothetical protein compare
PfGW456L13_4829 -1.0 -2.0 Predicted transcriptional regulator of N-Acetylglucosamine utilization, GntR family compare
PfGW456L13_4088 -1.0 -1.6 Transcriptional regulator, GntR family compare
PfGW456L13_4031 -1.0 -1.2 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
PfGW456L13_2342 -1.0 -2.6 SAM-dependent methyltransferases compare
PfGW456L13_2095 -0.9 -1.3 Ribonuclease E (EC 3.1.26.12) compare
PfGW456L13_4038 -0.9 -2.0 DNA topoisomerase I (EC 5.99.1.2) compare
PfGW456L13_990 -0.9 -1.6 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
PfGW456L13_4914 -0.9 -3.0 Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12) compare
PfGW456L13_3232 -0.9 -1.4 hypothetical protein compare
PfGW456L13_3227 -0.9 -2.7 ortholog of Bordetella pertussis (BX470248) BP2750 compare
PfGW456L13_2163 -0.9 -3.8 Rhodanese domain protein UPF0176 compare
PfGW456L13_4258 -0.9 -4.3 Chaperone protein HtpG compare
PfGW456L13_3894 -0.9 -0.6 Integration host factor alpha subunit compare
PfGW456L13_2804 -0.9 -3.0 Cytochrome c5 compare
PfGW456L13_4027 -0.9 -7.1 Transcription-repair coupling factor compare
PfGW456L13_350 -0.9 -3.0 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
PfGW456L13_75 -0.9 -2.0 Putative multicopper oxidases compare
PfGW456L13_2221 -0.9 -6.1 ErfK/YbiS/YcfS/YnhG family protein compare
PfGW456L13_4424 -0.9 -1.6 FIG00953416: hypothetical protein compare
PfGW456L13_5150 -0.9 -1.1 Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) compare
PfGW456L13_1009 -0.9 -0.6 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
PfGW456L13_3694 -0.9 -2.7 Arsenical resistance operon repressor compare
PfGW456L13_4506 -0.9 -2.8 Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor compare
PfGW456L13_4008 -0.9 -1.4 hypothetical protein compare
PfGW456L13_4308 -0.9 -1.9 Cytochrome c heme lyase subunit CcmH compare
PfGW456L13_3765 -0.9 -2.0 hypothetical protein compare
PfGW456L13_2897 -0.9 -0.9 hypothetical protein compare
PfGW456L13_2450 -0.8 -1.5 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
PfGW456L13_4934 -0.8 -3.3 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
PfGW456L13_3936 -0.8 -2.4 Colicin V production protein compare
PfGW456L13_161 -0.8 -4.9 putative transporter, required for glycine utilization (from data) compare
PfGW456L13_367 -0.8 -2.0 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
PfGW456L13_3016 -0.8 -4.1 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
PfGW456L13_4834 -0.8 -1.5 FIG137478: Hypothetical protein compare
PfGW456L13_5151 -0.8 -1.4 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
PfGW456L13_674 -0.8 -2.6 Chromosome (plasmid) partitioning protein ParB compare
PfGW456L13_267 -0.8 -2.4 hypothetical protein compare
PfGW456L13_839 -0.8 -2.2 FIG004064: hypothetical protein compare
PfGW456L13_3011 -0.8 -2.9 hypothetical protein compare
PfGW456L13_4108 -0.8 -1.6 CrfX protein compare
PfGW456L13_3443 -0.8 -2.3 putative cytochrome P450 hydroxylase compare
PfGW456L13_109 -0.8 -2.0 FIG00963947: hypothetical protein compare
PfGW456L13_3316 -0.8 -2.0 Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) compare
PfGW456L13_3000 -0.8 -1.3 FIG00961385: hypothetical protein compare
PfGW456L13_672 -0.8 -2.1 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
PfGW456L13_1842 -0.8 -4.0 Outer membrane porin, OprD family compare
PfGW456L13_2084 -0.8 -0.7 MotA/TolQ/ExbB proton channel family protein compare
PfGW456L13_2291 -0.8 -4.3 Periplasmic binding protein compare
PfGW456L13_5105 -0.8 -1.1 hypothetical protein compare
PfGW456L13_1300 -0.8 -1.8 dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) compare
PfGW456L13_1615 -0.8 -0.6 Probable transmembrane protein compare
PfGW456L13_2908 -0.8 -1.1 hypothetical protein compare
PfGW456L13_3313 -0.8 -2.7 FIG00957600: hypothetical protein compare
PfGW456L13_3047 -0.8 -1.0 Response regulator compare
PfGW456L13_2984 -0.8 -2.3 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
PfGW456L13_952 -0.8 -2.3 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
PfGW456L13_240 -0.8 -2.6 FIG143263: Glycosyl transferase compare
PfGW456L13_2435 -0.8 -2.0 Phenylacetic acid degradation protein PaaY compare
PfGW456L13_2032 -0.7 -2.1 Cysteine synthase B (EC 2.5.1.47) compare
PfGW456L13_3988 -0.7 -5.2 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
PfGW456L13_1628 -0.7 -2.5 ATPase, AFG1 family compare
PfGW456L13_787 -0.7 -3.2 Exodeoxyribonuclease III (EC 3.1.11.2) compare
PfGW456L13_4174 -0.7 -2.1 Glutaredoxin compare
PfGW456L13_491 -0.7 -4.5 Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1) compare
PfGW456L13_2597 -0.7 -2.3 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
PfGW456L13_4514 -0.7 -2.8 Sigma factor RpoE negative regulatory protein RseB precursor compare
PfGW456L13_2731 -0.7 -2.8 hypothetical protein compare
PfGW456L13_59 -0.7 -2.6 Sensor histidine kinase/response regulator compare
PfGW456L13_5218 -0.7 -1.2 tRNA-Pro-GGG compare
PfGW456L13_2196 -0.7 -2.8 FIG00956575: hypothetical protein compare
PfGW456L13_5135 -0.7 -3.5 Gluconate transporter family protein compare
PfGW456L13_1097 -0.7 -3.0 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
PfGW456L13_236 -0.7 -1.8 Acyl carrier protein (ACP1) compare
PfGW456L13_1108 -0.7 -1.2 hypothetical protein compare
PfGW456L13_2255 -0.7 -2.3 Organic hydroperoxide resistance transcriptional regulator compare
PfGW456L13_3029 -0.7 -3.4 Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-) compare
PfGW456L13_3538 -0.7 -2.9 FIG146518: Zn-dependent hydrolases, including glyoxylases compare
PfGW456L13_1689 -0.7 -1.7 Molybdenum cofactor biosynthesis protein MoaC compare
PfGW456L13_3955 -0.7 -2.8 Transcriptional regulator, TetR family compare
PfGW456L13_426 -0.7 -2.7 Osmolarity sensory histidine kinase EnvZ compare
PfGW456L13_1852 -0.7 -2.0 Holliday junction DNA helicase RuvA compare
PfGW456L13_794 -0.7 -4.0 Phosphomannomutase (EC 5.4.2.8) / Phosphoglucomutase (EC 5.4.2.2) compare
PfGW456L13_1409 -0.7 -3.3 ATP-dependent RNA helicase PA3950 compare
PfGW456L13_2336 -0.7 -2.0 O-antigen export system permease protein RfbD compare
PfGW456L13_873 -0.7 -2.9 Porphobilinogen synthase (EC 4.2.1.24) compare
PfGW456L13_254 -0.7 -1.1 hypothetical protein compare
PfGW456L13_3539 -0.7 -2.3 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
PfGW456L13_201 -0.7 -1.1 Hypothetical protein FIG015671 in large core OS assembly cluster compare
PfGW456L13_3138 -0.7 -1.0 phosphoesterase, putative compare
PfGW456L13_954 -0.7 -1.8 COG3332 compare
PfGW456L13_1370 -0.7 -1.5 FIG00957593: hypothetical protein compare
PfGW456L13_1022 -0.7 -1.5 twitching motility protein PilG compare
PfGW456L13_527 -0.7 -1.3 Transcriptional regulator compare
PfGW456L13_322 -0.7 -1.3 Histidine ABC transporter, ATP-binding protein (TC 3.A.1) compare
PfGW456L13_938 -0.7 -3.5 FIG00953078: hypothetical protein compare
PfGW456L13_3181 -0.7 -1.2 Organic hydroperoxide resistance protein compare
PfGW456L13_1834 -0.7 -2.8 Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1) compare


Specific Phenotypes

For 5 genes in this experiment

For stress PTLB purified from Pseudomonas sp. FW300-N2F2 in Pseudomonas fluorescens GW456-L13

For stress PTLB purified from Pseudomonas sp. FW300-N2F2 across organisms