Experiment set4IT027 for Pseudomonas fluorescens GW456-L13

Compare to:

KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x

Group: stress
Media: KB + PTLB purified from Pseudomonas sp. FW300-N2F2 (0.008 x) + Kanamycin sulfate (0.05 mg/mL), pH=7.2
Culturing: pseudo13_ML2a, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 4.5 generations
By: Sean Carim on 7/20/2018
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 5 genes in this experiment

For stress PTLB purified from Pseudomonas sp. FW300-N2F2 in Pseudomonas fluorescens GW456-L13

For stress PTLB purified from Pseudomonas sp. FW300-N2F2 across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance Efflux Pumps 1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 1
Thiamin biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I 2 2 1
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) 7 3 1
superpathway of thiamine diphosphate biosynthesis I 10 7 1
superpathway of thiamine diphosphate biosynthesis II 11 8 1