Experiment set4IT025 for Pseudomonas putida KT2440

LB with 3-methyl-3-butenol (pure) 0.25 vol%

Group: stress
Media: LB + 3-methyl-3-butenol (pure) (0.25 vol%)
Culturing: Putida_ML5, 48 well microplate; Tecan Safire, Aerobic, at 30 (C), shaken=orbital
Growth: about 6.8 generations
By: Mitch on 10/28/16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 3 genes in this experiment

For stress 3-methyl-3-butenol (pure) in Pseudomonas putida KT2440

For stress 3-methyl-3-butenol (pure) across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	1
Biogenesis of c-type cytochromes	1
Periplasmic disulfide interchange	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-arginine degradation II (AST pathway)	5	5	1