Experiment set4IT025 for Escherichia coli BW25113

Compare to:

LB with 3-methyl-1-butanol 0.625 vol%

200 most important genes:

  gene name fitness t score description  
b1095 fabF -4.7 -11.1 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0630 lipB -3.8 -2.6 protein of lipoate biosynthesis (VIMSS) compare
b3839 tatC -3.1 -12.7 TatABCE protein translocation system subunit (NCBI) compare
b3405 ompR -3.1 -7.1 osmolarity response regulator (NCBI) compare
b0014 dnaK -3.1 -3.6 molecular chaperone DnaK (NCBI) compare
b0114 aceE -2.9 -2.0 pyruvate dehydrogenase subunit E1 (NCBI) compare
b3095 yqjA -2.9 -9.4 conserved inner membrane protein (NCBI) compare
b2297 pta -2.9 -1.9 phosphate acetyltransferase (NCBI) compare
b3398 yrfF -2.8 -4.9 predicted inner membrane protein (NCBI) compare
b3290 trkA -2.8 -4.6 potassium transporter peripheral membrane component (NCBI) compare
b0200 gmhB -2.6 -3.0 hypothetical protein (NCBI) compare
b3613 yibP -2.5 -8.9 putative membrane protein (VIMSS) compare
b0053 surA -2.5 -7.2 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1712 ihfA -2.4 -5.0 integration host factor subunit alpha (NCBI) compare
b3493 pitA -2.4 -11.8 phosphate transporter, low-affinity (NCBI) compare
b3462 ftsX -2.4 -8.8 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0149 mrcB -2.3 -15.0 penicillin-binding protein 1b (NCBI) compare
b3916 pfkA -2.3 -6.0 6-phosphofructokinase (NCBI) compare
b3621 rfaC -2.2 -7.2 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b4239 treC -2.2 -8.0 trehalose-6-P hydrolase (NCBI) compare
b1109 ndh -2.2 -6.6 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b1292 sapC -2.2 -4.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3390 aroK -2.2 -3.4 shikimate kinase I (NCBI) compare
b3619 rfaD -2.1 -5.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3838 tatB -2.1 -7.6 sec-independent translocase (NCBI) compare
b3386 rpe -2.1 -1.4 ribulose-phosphate 3-epimerase (NCBI) compare
b2935 tktA -2.0 -4.4 transketolase 1, thiamin-binding (NCBI) compare
b0019 nhaA -2.0 -9.4 pH-dependent sodium/proton antiporter (NCBI) compare
b3052 rfaE -2.0 -6.8 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3863 polA -1.9 -2.2 DNA polymerase I (NCBI) compare
b1290 sapF -1.9 -3.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1236 galU -1.9 -7.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2620 smpB -1.9 -4.1 SsrA-binding protein (NCBI) compare
b3463 ftsE -1.9 -6.0 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3426 glpD -1.9 -8.6 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b3725 pstB -1.9 -3.8 phosphate transporter subunit (NCBI) compare
b3933 ftsN -1.9 -11.0 essential cell division protein (NCBI) compare
b3414 gntY -1.9 -7.4 predicted gluconate transport associated protein (NCBI) compare
b0439 lon -1.8 -6.7 DNA-binding ATP-dependent protease La (NCBI) compare
b1105 ycfM -1.8 -4.7 predicted outer membrane lipoprotein (NCBI) compare
b1291 sapD -1.7 -3.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0429 cyoD -1.7 -6.1 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b3849 trkH -1.6 -10.7 potassium transporter (NCBI) compare
b2820 recB -1.6 -3.6 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0049 apaH -1.6 -6.7 diadenosinetetraphosphatase (NCBI) compare
b3842 rfaH -1.5 -5.8 transcriptional activator RfaH (NCBI) compare
b0065 yabI -1.5 -6.0 conserved inner membrane protein (NCBI) compare
b0161 degP -1.5 -4.9 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b3404 envZ -1.5 -8.8 osmolarity sensor protein (NCBI) compare
b0015 dnaJ -1.4 -8.8 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b2279 nuoK -1.4 -2.9 NADH dehydrogenase subunit K (NCBI) compare
b0683 fur -1.4 -2.2 ferric uptake regulator (NCBI) compare
b3631 rfaG -1.4 -11.7 glucosyltransferase I (NCBI) compare
b3204 ptsN -1.4 -5.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2175 spr -1.4 -3.9 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b4222 ytfP -1.4 -4.9 hypothetical protein (NCBI) compare
b0428 cyoE -1.4 -6.8 protoheme IX farnesyltransferase (NCBI) compare
b0145 dksA -1.3 -4.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0890 ftsK -1.3 -7.6 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3630 rfaP -1.3 -9.7 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0222 lpcA -1.3 -3.2 phosphoheptose isomerase (NCBI) compare
b0738 tolR -1.3 -3.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3912 cpxR -1.3 -8.3 DNA-binding response regulator in two-component regulatory system with CpxA (NCBI) compare
b3811 xerC -1.3 -3.1 site-specific tyrosine recombinase XerC (NCBI) compare
b2567 rnc -1.2 -2.2 ribonuclease III (NCBI) compare
b2330 yfcB -1.2 -2.4 putative adenine-specific methylase (VIMSS) compare
b2318 truA -1.2 -4.4 tRNA pseudouridine synthase A (NCBI) compare
b2688 gshA -1.2 -7.3 glutamate--cysteine ligase (NCBI) compare
b3609 secB -1.2 -2.4 export protein SecB (NCBI) compare
b0431 cyoB -1.2 -8.5 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b3620 rfaF -1.2 -5.1 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2830 nudH -1.2 -2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b0430 cyoC -1.2 -4.0 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b2314 dedD -1.2 -3.3 putative lipoprotein (VIMSS) compare
b2910 zapA -1.1 -4.5 protein that localizes to the cytokinetic ring (NCBI) compare
b3836 b3836 -1.1 -2.3 orf, hypothetical protein (VIMSS) compare
b2614 grpE -1.1 -2.0 heat shock protein (NCBI) compare
b0912 ihfB -1.1 -3.5 integration host factor subunit beta (NCBI) compare
b4025 pgi -1.1 -6.9 glucose-6-phosphate isomerase (NCBI) compare
b2340 sixA -1.1 -3.3 phosphohistidine phosphatase (NCBI) compare
b1863 ruvC -1.1 -3.8 Holliday junction resolvase (NCBI) compare
b4042 dgkA -1.1 -3.5 diacylglycerol kinase (NCBI) compare
b3162 deaD -1.1 -5.3 inducible ATP-independent RNA helicase (VIMSS) compare
b1183 umuD -1.1 -2.6 DNA polymerase V, subunit D (NCBI) compare
b3726 pstA -1.1 -5.5 phosphate transporter subunit (NCBI) compare
b0957 ompA -1.1 -3.0 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b4472 yhdP -1.1 -9.3 conserved membrane protein, predicted transporter (NCBI) compare
b2906 visC -1.1 -5.2 hypothetical protein (NCBI) compare
b2894 xerD -1.1 -1.7 site-specific tyrosine recombinase XerD (NCBI) compare
b1175 minD -1.0 -4.6 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3169 nusA -1.0 -2.8 transcription elongation factor NusA (NCBI) compare
b2947 gshB -1.0 -3.8 glutathione synthetase (NCBI) compare
b3042 b3042 -1.0 -2.1 orf, hypothetical protein (VIMSS) compare
b3237 argR -1.0 -4.5 arginine repressor (NCBI) compare
b0432 cyoA -1.0 -6.9 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b4155 yjeA -1.0 -1.6 putative lysyl-tRNA synthetase (VIMSS) compare
b0926 ycbK -1.0 -2.8 hypothetical protein (NCBI) compare
b4699 fnrS -1.0 -1.2 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1167 ymgC -1.0 -1.8 hypothetical protein (NCBI) compare
b3387 dam -1.0 -7.6 DNA adenine methylase (NCBI) compare
b1192 ldcA -0.9 -4.5 L,D-carboxypeptidase A (NCBI) compare
b2058 wcaB -0.9 -1.2 predicted acyl transferase (NCBI) compare
b0080 fruR -0.9 -4.9 DNA-binding transcriptional dual regulator (NCBI) compare
b4375 prfC -0.9 -5.5 peptide chain release factor 3 (NCBI) compare
b2699 recA -0.9 -2.3 recombinase A (NCBI) compare
b2528 iscA -0.9 -1.9 iron-sulfur cluster assembly protein (NCBI) compare
b3465 yhhF -0.9 -4.2 predicted methyltransferase (NCBI) compare
b0843 ybjH -0.9 -2.4 hypothetical protein (NCBI) compare
b1514 lsrC -0.9 -2.1 AI2 transporter (NCBI) compare
b0441 ppiD -0.9 -6.6 peptidyl-prolyl cis-trans isomerase (rotamase D) (NCBI) compare
b3261 fis -0.9 -2.5 DNA-binding protein Fis (NCBI) compare
b2822 recC -0.9 -3.7 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b0920 ycbC -0.8 -3.3 conserved inner membrane protein (NCBI) compare
b3611 yibN -0.8 -3.0 predicted rhodanese-related sulfurtransferase (NCBI) compare
b2276 nuoN -0.8 -4.1 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b1840 yebZ -0.8 -3.7 predicted inner membrane protein (NCBI) compare
b2287 nuoB -0.8 -2.9 NADH dehydrogenase subunit B (NCBI) compare
b1379 hslJ -0.8 -3.0 heat-inducible protein (NCBI) compare
b4451 ryhB -0.8 -1.3 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2908 pepP -0.8 -4.3 proline aminopeptidase P II (NCBI) compare
b1627 rsxA -0.8 -3.1 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b3742 mioC -0.8 -3.2 flavodoxin (NCBI) compare
b3706 trmE -0.7 -4.1 tRNA modification GTPase (NCBI) compare
b3807 cyaY -0.7 -4.5 frataxin-like protein (NCBI) compare
b2937 speB -0.7 -4.5 agmatinase (NCBI) compare
b0579 ybdF -0.7 -2.2 hypothetical protein (NCBI) compare
b2512 yfgL -0.7 -3.2 protein assembly complex, lipoprotein component (NCBI) compare
b0767 ybhE -0.7 -3.2 6-phosphogluconolactonase (NCBI) compare
b2278 nuoL -0.7 -5.1 NADH dehydrogenase subunit L (NCBI) compare
b2188 yejM -0.7 -4.9 predicted hydrolase, inner membrane (NCBI) compare
b0232 yafN -0.7 -0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4460 araH -0.7 -2.6 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1176 minC -0.7 -2.8 septum formation inhibitor (NCBI) compare
b2352 yfdI -0.7 -2.8 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b3935 priA -0.7 -1.5 primosome assembly protein PriA (NCBI) compare
b1855 lpxM -0.7 -2.2 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0427 yajR -0.7 -4.8 putative transport protein (VIMSS) compare
b2572 rseA -0.7 -2.3 anti-sigma factor (NCBI) compare
b3931 hslU -0.7 -5.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1235 rssB -0.7 -4.3 response regulator of RpoS (NCBI) compare
b1693 aroD -0.7 -3.4 3-dehydroquinate dehydratase (NCBI) compare
b3806 cyaA -0.7 -3.0 adenylate cyclase (NCBI) compare
b1426 ydcH -0.7 -1.3 orf, hypothetical protein (VIMSS) compare
b2277 nuoM -0.7 -3.8 NADH dehydrogenase subunit M (NCBI) compare
b3650 spoT -0.7 -2.5 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b4413 sokC -0.7 -1.4 regulatory antisense RNA (NCBI) compare
b0226 dinJ -0.7 -1.7 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b2280 nuoJ -0.7 -3.2 NADH dehydrogenase subunit J (NCBI) compare
b2518 ndk -0.7 -1.2 nucleoside diphosphate kinase (NCBI) compare
b0814 ompX -0.6 -2.6 outer membrane protein X (NCBI) compare
b1038 csgF -0.6 -2.6 predicted transport protein (NCBI) compare
b2282 nuoH -0.6 -2.1 NADH dehydrogenase subunit H (NCBI) compare
b2025 hisF -0.6 -2.4 imidazole glycerol phosphate synthase subunit HisF (NCBI) compare
b1305 pspB -0.6 -2.2 phage shock protein B (NCBI) compare
b2286 nuoC -0.6 -2.7 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b1630 rsxD -0.6 -3.7 electron transport complex protein RnfD (NCBI) compare
b2283 nuoG -0.6 -4.0 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b4438 cyaR -0.6 -1.9 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b0784 moaD -0.6 -1.8 molybdopterin synthase, small subunit (NCBI) compare
b1388 paaA -0.6 -2.6 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b3545 proK -0.6 -2.0 tRNA-Pro (NCBI) compare
b1187 fadR -0.6 -3.0 fatty acid metabolism regulator (NCBI) compare
b0450 glnK -0.6 -1.5 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b1811 yoaH -0.6 -2.1 hypothetical protein (NCBI) compare
b1273 yciN -0.6 -1.9 hypothetical protein (NCBI) compare
b0688 pgm -0.6 -2.0 phosphoglucomutase (NCBI) compare
b3834 yigP -0.6 -3.1 hypothetical protein (NCBI) compare
b2204 napH -0.6 -2.6 quinol dehydrogenase membrane component (NCBI) compare
b3632 rfaQ -0.6 -5.4 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4644 ypjI -0.6 -2.0 no description compare
b0263 afuB -0.6 -1.7 CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq) compare
b2285 nuoE -0.6 -1.3 NADH dehydrogenase subunit E (NCBI) compare
b3228 sspB -0.6 -2.2 ClpXP protease specificity-enhancing factor (NCBI) compare
b0458 ylaC -0.6 -3.0 orf, hypothetical protein (VIMSS) compare
b1045 ymdB -0.6 -2.2 hypothetical protein (NCBI) compare
b1084 rne -0.6 -2.0 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2052 fcl -0.6 -2.9 bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI) compare
b1293 sapB -0.6 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2113 mrp -0.6 -2.9 putative ATPase (VIMSS) compare
b0739 tolA -0.6 -2.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3870 glnA -0.6 -1.9 glutamine synthetase (NCBI) compare
b1836 yebV -0.6 -2.1 orf, hypothetical protein (VIMSS) compare
b3781 trxA -0.6 -1.8 thioredoxin 1 (VIMSS) compare
b1332 ynaJ -0.6 -0.8 predicted inner membrane protein (NCBI) compare
b3727 pstC -0.6 -2.2 phosphate transporter subunit (NCBI) compare
b1890 motA -0.6 -2.5 flagellar motor protein MotA (NCBI) compare
b0167 glnD -0.6 -2.2 PII uridylyl-transferase (NCBI) compare
b1658 purR -0.6 -2.8 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b3932 hslV -0.6 -3.8 ATP-dependent protease peptidase subunit (NCBI) compare
b1083 flgL -0.6 -2.7 flagellar hook-associated protein L (NCBI) compare
b1347 ydaC -0.5 -1.5 Rac prophage; predicted protein (NCBI) compare
b2811 ygdK -0.5 -2.1 predicted Fe-S metabolism protein (NCBI) compare
b2229 b2229 -0.5 -1.6 orf, hypothetical protein (VIMSS) compare
b4505 insX -0.5 -1.3 predicted IS protein (RefSeq) compare
b3741 gidA -0.5 -3.4 glucose-inhibited division protein A (NCBI) compare
b2564 pdxJ -0.5 -2.3 pyridoxal phosphate biosynthetic protein (NCBI) compare
b1101 ptsG -0.5 -4.1 fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI) compare
b3636 rpmG -0.5 -0.6 50S ribosomal protein L33 (NCBI) compare
b3940 metL -0.5 -3.7 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b4685 yrbN -0.5 -1.3 expressed protein (RefSeq) compare


Specific Phenotypes

For 5 genes in this experiment

For stress 3-methyl-1-butanol in Escherichia coli BW25113

For stress 3-methyl-1-butanol across organisms