Experiment set4IT023 for Escherichia coli BW25113

LB with 3-methyl-1-butanol 1.25 vol%

Group: stress
Media: LB + 3-methyl-1-butanol (1.25 vol%)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Mitch on 3/15/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 11 genes in this experiment

For stress 3-methyl-1-butanol in Escherichia coli BW25113

For stress 3-methyl-1-butanol across organisms

SEED Subsystems

Subsystem	#Specific
Proteasome bacterial	2
Proteolysis in bacteria, ATP-dependent	2
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Nudix proteins (nucleoside triphosphate hydrolases)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Aminophosphonate metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Glycerophospholipid metabolism
• Sphingolipid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cardiolipin biosynthesis I	3	3	1
cardiolipin biosynthesis II	3	3	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	2
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1