Experiment set4IT018 for Escherichia coli BW25113

Compare to:

LB with 3-methyl-2-butenol 0.625 vol%

200 most important genes:

  gene name fitness t score description  
b0630 lipB -3.7 -2.5 protein of lipoate biosynthesis (VIMSS) compare
b3414 gntY -3.7 -7.8 predicted gluconate transport associated protein (NCBI) compare
b0200 gmhB -3.4 -2.3 hypothetical protein (NCBI) compare
b2894 xerD -3.2 -3.1 site-specific tyrosine recombinase XerD (NCBI) compare
b3493 pitA -3.2 -11.7 phosphate transporter, low-affinity (NCBI) compare
b0439 lon -3.0 -7.2 DNA-binding ATP-dependent protease La (NCBI) compare
b3290 trkA -3.0 -3.6 potassium transporter peripheral membrane component (NCBI) compare
b0053 surA -2.9 -7.5 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3863 polA -2.8 -1.9 DNA polymerase I (NCBI) compare
b0683 fur -2.7 -3.2 ferric uptake regulator (NCBI) compare
b2935 tktA -2.7 -5.0 transketolase 1, thiamin-binding (NCBI) compare
b3052 rfaE -2.5 -7.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3621 rfaC -2.5 -7.3 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3390 aroK -2.5 -3.6 shikimate kinase I (NCBI) compare
b2699 recA -2.5 -3.6 recombinase A (NCBI) compare
b2330 yfcB -2.4 -4.2 putative adenine-specific methylase (VIMSS) compare
b1290 sapF -2.3 -3.4 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2820 recB -2.3 -4.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1291 sapD -2.3 -3.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1175 minD -2.3 -9.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3619 rfaD -2.2 -5.4 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2436 hemF -2.2 -5.1 coproporphyrinogen III oxidase (NCBI) compare
b0114 aceE -2.2 -2.9 pyruvate dehydrogenase subunit E1 (NCBI) compare
b4172 hfq -2.2 -2.8 RNA-binding protein Hfq (NCBI) compare
b3463 ftsE -2.2 -6.1 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b3725 pstB -2.1 -4.4 phosphate transporter subunit (NCBI) compare
b2688 gshA -2.1 -9.8 glutamate--cysteine ligase (NCBI) compare
b1236 galU -2.1 -7.8 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3169 nusA -2.0 -2.7 transcription elongation factor NusA (NCBI) compare
b3842 rfaH -2.0 -7.1 transcriptional activator RfaH (NCBI) compare
b0890 ftsK -2.0 -9.5 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1712 ihfA -1.9 -4.5 integration host factor subunit alpha (NCBI) compare
b3839 tatC -1.9 -10.3 TatABCE protein translocation system subunit (NCBI) compare
b0688 pgm -1.9 -5.1 phosphoglucomutase (NCBI) compare
b3386 rpe -1.8 -1.7 ribulose-phosphate 3-epimerase (NCBI) compare
b3811 xerC -1.8 -4.5 site-specific tyrosine recombinase XerC (NCBI) compare
b3916 pfkA -1.8 -5.4 6-phosphofructokinase (NCBI) compare
b1861 ruvA -1.8 -2.3 Holliday junction DNA helicase motor protein (NCBI) compare
b3630 rfaP -1.8 -11.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b3462 ftsX -1.7 -7.1 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1109 ndh -1.7 -5.6 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b0222 lpcA -1.7 -3.5 phosphoheptose isomerase (NCBI) compare
b2947 gshB -1.7 -6.2 glutathione synthetase (NCBI) compare
b3426 glpD -1.7 -8.1 sn-glycerol-3-phosphate dehydrogenase, aerobic, FAD/NAD(P)-binding (NCBI) compare
b3631 rfaG -1.6 -13.5 glucosyltransferase I (NCBI) compare
b3620 rfaF -1.6 -5.8 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3162 deaD -1.6 -6.8 inducible ATP-independent RNA helicase (VIMSS) compare
b3636 rpmG -1.6 -2.7 50S ribosomal protein L33 (NCBI) compare
b3613 yibP -1.6 -6.8 putative membrane protein (VIMSS) compare
b4239 treC -1.6 -6.8 trehalose-6-P hydrolase (NCBI) compare
b1292 sapC -1.6 -3.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4375 prfC -1.5 -7.9 peptide chain release factor 3 (NCBI) compare
b0049 apaH -1.5 -5.7 diadenosinetetraphosphatase (NCBI) compare
b3961 oxyR -1.5 -10.0 DNA-binding transcriptional dual regulator (NCBI) compare
b1293 sapB -1.5 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1176 minC -1.5 -5.0 septum formation inhibitor (NCBI) compare
b0912 ihfB -1.4 -4.1 integration host factor subunit beta (NCBI) compare
b3778 rep -1.4 -8.7 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b2392 mntH -1.4 -7.6 manganese transport protein MntH (NCBI) compare
b4155 yjeA -1.3 -1.9 putative lysyl-tRNA synthetase (VIMSS) compare
b2620 smpB -1.3 -3.1 SsrA-binding protein (NCBI) compare
b1095 fabF -1.3 -7.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b3979 thrT -1.3 -1.5 tRNA-Thr (NCBI) compare
b2822 recC -1.3 -5.2 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b3742 mioC -1.3 -5.4 flavodoxin (NCBI) compare
b2567 rnc -1.3 -2.1 ribonuclease III (NCBI) compare
b2297 pta -1.3 -2.1 phosphate acetyltransferase (NCBI) compare
b0428 cyoE -1.3 -6.6 protoheme IX farnesyltransferase (NCBI) compare
b3405 ompR -1.3 -4.9 osmolarity response regulator (NCBI) compare
b0149 mrcB -1.3 -9.2 penicillin-binding protein 1b (NCBI) compare
b4585 sroB -1.2 -1.7 Novel sRNA, function unknown (RefSeq) compare
b3807 cyaY -1.2 -6.3 frataxin-like protein (NCBI) compare
b2814 metZ -1.2 -2.0 tRNA-Met (NCBI) compare
b4407 thiS -1.2 -2.0 sulfur carrier protein ThiS (NCBI) compare
b1855 lpxM -1.1 -3.6 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0118 acnB -1.1 -3.6 aconitate hydratase (NCBI) compare
b3838 tatB -1.1 -5.0 sec-independent translocase (NCBI) compare
b3632 rfaQ -1.1 -10.0 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2906 visC -1.1 -5.5 hypothetical protein (NCBI) compare
b0432 cyoA -1.0 -6.6 cytochrome o ubiquinol oxidase subunit II (NCBI) compare
b0767 ybhE -1.0 -4.3 6-phosphogluconolactonase (NCBI) compare
b0880 cspD -1.0 -2.3 cold shock protein homolog (NCBI) compare
b1860 ruvB -1.0 -1.3 Holliday junction DNA helicase B (NCBI) compare
b3649 rpoZ -1.0 -3.3 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3871 bipA -1.0 -7.4 GTP-binding protein (NCBI) compare
b3849 trkH -1.0 -7.5 potassium transporter (NCBI) compare
b3164 pnp -1.0 -3.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0014 dnaK -1.0 -2.1 molecular chaperone DnaK (NCBI) compare
b0356 frmA -1.0 -5.6 alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI) compare
b1818 manY -1.0 -2.5 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1863 ruvC -1.0 -3.3 Holliday junction resolvase (NCBI) compare
b0431 cyoB -1.0 -6.5 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b3009 yghB -1.0 -4.4 conserved inner membrane protein (NCBI) compare
b2528 iscA -0.9 -1.8 iron-sulfur cluster assembly protein (NCBI) compare
b1105 ycfM -0.9 -2.8 predicted outer membrane lipoprotein (NCBI) compare
b1693 aroD -0.9 -5.4 3-dehydroquinate dehydratase (NCBI) compare
b3741 gidA -0.9 -5.9 glucose-inhibited division protein A (NCBI) compare
b4577 sgrS -0.9 -1.7 small antisense RNA (NCBI) compare
b3341 rpsG -0.9 -1.4 30S ribosomal protein S7 (NCBI) compare
b4546 ypeB -0.9 -1.5 hypothetical protein (NCBI) compare
b3237 argR -0.9 -4.2 arginine repressor (NCBI) compare
b3261 fis -0.9 -2.6 DNA-binding protein Fis (NCBI) compare
b4685 yrbN -0.9 -2.0 expressed protein (RefSeq) compare
b3609 secB -0.9 -3.1 export protein SecB (NCBI) compare
b3017 sufI -0.9 -6.4 repressor protein for FtsI (NCBI) compare
b3935 priA -0.9 -1.7 primosome assembly protein PriA (NCBI) compare
b3095 yqjA -0.9 -5.2 conserved inner membrane protein (NCBI) compare
b2784 relA -0.9 -5.8 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b3933 ftsN -0.9 -6.1 essential cell division protein (NCBI) compare
b2113 mrp -0.9 -3.9 putative ATPase (VIMSS) compare
b3761 trpT -0.9 -1.7 tRNA-Trp (NCBI) compare
b3977 tyrU -0.8 -0.9 tRNA-Tyr (NCBI) compare
b2058 wcaB -0.8 -1.1 predicted acyl transferase (NCBI) compare
b0018 mokC -0.8 -1.2 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b0161 degP -0.8 -5.1 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b4177 purA -0.8 -3.1 adenylosuccinate synthetase (NCBI) compare
b2314 dedD -0.8 -2.9 putative lipoprotein (VIMSS) compare
b0677 nagA -0.8 -4.5 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b1493 gadB -0.8 -1.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1681 sufD -0.8 -4.3 component of SufBCD complex (NCBI) compare
b3465 yhhF -0.8 -4.3 predicted methyltransferase (NCBI) compare
b1550 b1550 -0.8 -1.3 orf, hypothetical protein (VIMSS) compare
b0726 sucA -0.8 -2.9 alpha-ketoglutarate decarboxylase (NCBI) compare
b1412 azoR -0.8 -1.6 acyl carrier protein phosphodiesterase (NCBI) compare
b3387 dam -0.8 -5.8 DNA adenine methylase (NCBI) compare
b3204 ptsN -0.8 -3.2 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1305 pspB -0.8 -2.4 phage shock protein B (NCBI) compare
b4527 b1354 -0.7 -3.0 orf, hypothetical protein (VIMSS) compare
b3706 trmE -0.7 -4.3 tRNA modification GTPase (NCBI) compare
b2435 amiA -0.7 -2.7 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b2114 metG -0.7 -1.6 methionyl-tRNA synthetase (NCBI) compare
b3107 yhaL -0.7 -1.3 orf, hypothetical protein (VIMSS) compare
b2278 nuoL -0.7 -5.1 NADH dehydrogenase subunit L (NCBI) compare
b1426 ydcH -0.7 -1.4 orf, hypothetical protein (VIMSS) compare
b0430 cyoC -0.7 -2.6 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b2576 srmB -0.7 -2.7 ATP-dependent RNA helicase (NCBI) compare
b0143 pcnB -0.7 -4.2 poly(A) polymerase I (VIMSS) compare
b0429 cyoD -0.7 -2.8 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b3627 rfaI -0.7 -6.0 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2277 nuoM -0.7 -3.8 NADH dehydrogenase subunit M (NCBI) compare
b0167 glnD -0.7 -2.8 PII uridylyl-transferase (NCBI) compare
b3398 yrfF -0.7 -1.4 predicted inner membrane protein (NCBI) compare
b3027 ygiZ -0.7 -2.3 conserved inner membrane protein (NCBI) compare
b0817 mntR -0.7 -2.9 DNA-binding transcriptional regulator of mntH (NCBI) compare
b2340 sixA -0.7 -2.8 phosphohistidine phosphatase (NCBI) compare
b0888 trxB -0.7 -2.1 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b2280 nuoJ -0.7 -3.3 NADH dehydrogenase subunit J (NCBI) compare
b2945 endA -0.7 -2.9 DNA-specific endonuclease I (NCBI) compare
b1644 b1644 -0.7 -2.2 putative membrane protein (VIMSS) compare
b3803 hemX -0.7 -4.8 predicted uroporphyrinogen III methylase (NCBI) compare
b2450 yffS -0.7 -1.4 CPZ-55 prophage; predicted protein (RefSeq) compare
b1954 dsrA -0.6 -1.8 regulatory antisense RNA (NCBI) compare
b3806 cyaA -0.6 -3.5 adenylate cyclase (NCBI) compare
b2811 ygdK -0.6 -3.1 predicted Fe-S metabolism protein (NCBI) compare
b1664 ydhQ -0.6 -3.6 hypothetical protein (NCBI) compare
b0464 acrR -0.6 -5.1 DNA-binding transcriptional repressor (NCBI) compare
b1486 ddpB -0.6 -1.8 D-ala-D-ala transporter subunit (NCBI) compare
b1235 rssB -0.6 -4.1 response regulator of RpoS (NCBI) compare
b0209 yafD -0.6 -3.1 hypothetical protein (NCBI) compare
b1187 fadR -0.6 -3.0 fatty acid metabolism regulator (NCBI) compare
b2431 b2431 -0.6 -4.1 orf, hypothetical protein (VIMSS) compare
b0427 yajR -0.6 -3.9 putative transport protein (VIMSS) compare
b4648 ysaC -0.6 -2.0 no description compare
b3813 uvrD -0.6 -5.3 DNA-dependent ATPase I and helicase II (NCBI) compare
b0226 dinJ -0.6 -1.5 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4631 ykgQ -0.6 -1.3 no description compare
b0720 gltA -0.6 -2.5 citrate synthase (NCBI) compare
b1918 yecS -0.6 -2.2 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2895 fldB -0.6 -2.1 flavodoxin 2 (NCBI) compare
b3628 rfaB -0.6 -5.2 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b0082 mraW -0.6 -2.5 S-adenosyl-methyltransferase (NCBI) compare
b4402 yjjY -0.6 -1.1 hypothetical protein (NCBI) compare
b2287 nuoB -0.6 -2.3 NADH dehydrogenase subunit B (NCBI) compare
b1671 ydhX -0.6 -1.6 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b2531 iscR -0.6 -0.8 DNA-binding transcriptional repressor (NCBI) compare
b1253 yciA -0.6 -1.9 predicted hydrolase (NCBI) compare
b2285 nuoE -0.6 -1.4 NADH dehydrogenase subunit E (NCBI) compare
b1160 elbA -0.6 -1.9 hypothetical protein (NCBI) compare
b4529 ydbJ -0.6 -0.8 hypothetical protein (NCBI) compare
b4222 ytfP -0.6 -2.7 hypothetical protein (NCBI) compare
b2447 yffP -0.6 -2.3 CPZ-55 prophage; predicted protein (NCBI) compare
b0812 dps -0.6 -2.9 DNA protection during starvation conditions (NCBI) compare
b1097 yceG -0.6 -2.9 predicted aminodeoxychorismate lyase (NCBI) compare
b1984 asnW -0.6 -0.8 tRNA-Asn (NCBI) compare
b4202 rpsR -0.6 -1.1 30S ribosomal protein S18 (NCBI) compare
b2419 yfeK -0.6 -1.5 hypothetical protein (NCBI) compare
b1658 purR -0.6 -2.8 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b2318 truA -0.6 -2.3 tRNA pseudouridine synthase A (NCBI) compare
b0843 ybjH -0.6 -1.9 hypothetical protein (NCBI) compare
b3438 gntR -0.6 -2.7 DNA-binding transcriptional repressor (NCBI) compare
b1380 ldhA -0.6 -1.4 D-lactate dehydrogenase (NCBI) compare
b3652 recG -0.6 -4.0 ATP-dependent DNA helicase (NCBI) compare
b4025 pgi -0.6 -3.9 glucose-6-phosphate isomerase (NCBI) compare
b3293 yhdN -0.5 -2.4 hypothetical protein (NCBI) compare
b0957 ompA -0.5 -2.0 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0965 b0965 -0.5 -1.3 orf, hypothetical protein (VIMSS) compare
b3181 greA -0.5 -2.6 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b0418 pgpA -0.5 -2.6 phosphatidylglycerophosphatase A (NCBI) compare
b2284 nuoF -0.5 -2.4 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b0955 ycbZ -0.5 -3.6 predicted peptidase (NCBI) compare


Specific Phenotypes

For 3 genes in this experiment

For stress 3-methyl-2-butenol in Escherichia coli BW25113

For stress 3-methyl-2-butenol across organisms