Experiment set4IT015 for Escherichia coli BW25113

LB with 3-methyl-2-butenol 1.25 vol%

Group: stress
Media: LB + 3-methyl-2-butenol (1.25 vol%)
Culturing: Keio_ML9, 48 well microplate; Tecan Infinite F200, Aerobic, at 28 (C), shaken=orbital
By: Mitch on 3/15/2015
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 11 genes in this experiment

For stress 3-methyl-2-butenol in Escherichia coli BW25113

For stress 3-methyl-2-butenol across organisms

SEED Subsystems

Subsystem	#Specific
Glutathione-dependent pathway of formaldehyde detoxification	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Oxidative stress	1
Transport of Manganese	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Aminophosphonate metabolism
• Selenoamino acid metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Glycerophospholipid metabolism
• Sphingolipid metabolism
• Methane metabolism
• Thiamine metabolism
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine degradation IV	1	1	1
L-alanine biosynthesis III	1	1	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
cardiolipin biosynthesis I	3	3	1
formaldehyde oxidation II (glutathione-dependent)	3	3	1
cardiolipin biosynthesis II	3	3	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
thiazole component of thiamine diphosphate biosynthesis II	7	5	2
superpathway of L-alanine biosynthesis	4	4	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
superpathway of thiamine diphosphate biosynthesis II	11	9	2
molybdopterin biosynthesis	6	6	1
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	2
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
superpathway of thiamine diphosphate biosynthesis I	10	10	1
tRNA-uridine 2-thiolation and selenation (bacteria)	11	7	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1
superpathway of C1 compounds oxidation to CO2	12	4	1