Experiment set4IT005 for Escherichia coli BL21
Mixed culture, Bdellovibrio bacteriovorus HD100_1
Group: baloMedia: M9 (0.1x) + CaCl2, MgSO4 (5mM ), pH=7
Culturing: BL21_ML4, 48-well_plate, Aerobic, at 30C (C), shaken=700 rpm
By: AR on 5/19/21
Media components: 0.4 g/L D-Glucose, 0.2 mM Magnesium sulfate, 0.01 mM Calcium chloride, 1.28 g/L Sodium phosphate dibasic heptahydrate, 0.3 g/L Potassium phosphate monobasic, 0.05 g/L Sodium Chloride, 0.1 g/L Ammonium chloride (final concentrations)
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Formate hydrogenase | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-serine degradation | 3 | 3 | 3 |
| L-tryptophan degradation II (via pyruvate) | 3 | 3 | 2 |
| D-serine degradation | 3 | 3 | 2 |
| L-cysteine degradation II | 3 | 2 | 2 |
| glycine betaine degradation III | 7 | 4 | 3 |
| felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis | 5 | 2 | 2 |
| glycine betaine degradation I | 8 | 4 | 3 |
| glycine degradation | 3 | 3 | 1 |
| L-methionine biosynthesis II | 6 | 5 | 2 |
| L-mimosine degradation | 8 | 4 | 2 |
| glutathione-mediated detoxification I | 8 | 3 | 2 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 2 |
| purine nucleobases degradation II (anaerobic) | 24 | 17 | 3 |