Experiment set47S238 for Pseudomonas putida KT2440
mixed carbon sources Trisodium citrate dihydrate 10 mM and Parachlorophenylalanine 10 mM
Group: mixed carbon sourceMedia: MME_noCarbon + Trisodium citrate dihydrate (10 mM) + Parachlorophenylalanine (10 mM)
Culturing: Putida_ML5_PNNL, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 11/17/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 8 genes in this experiment
For mixed carbon source Trisodium citrate dihydrate in Pseudomonas putida KT2440
For mixed carbon source Trisodium citrate dihydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Aromatic amino acid degradation | 1 |
| Auxin biosynthesis | 1 |
| Di-Inositol-Phosphate biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Histidine metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- Tryptophan metabolism
- Streptomycin biosynthesis
- Inositol phosphate metabolism
- Indole and ipecac alkaloid biosynthesis
- Alkaloid biosynthesis I
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Phosphatidylinositol signaling system
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: