Experiment set47S234 for Pseudomonas putida KT2440
mixed carbon sources Trisodium citrate dihydrate 10 mM and L-Meta-tyrosine 10 mM
Group: mixed carbon sourceMedia: MME_noCarbon + Trisodium citrate dihydrate (10 mM) + L-Meta-tyrosine (10 mM)
Culturing: Putida_ML5_PNNL, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 11/17/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 7 genes in this experiment
For mixed carbon source Trisodium citrate dihydrate in Pseudomonas putida KT2440
For mixed carbon source Trisodium citrate dihydrate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Phenylalanine metabolism
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-phenylalanine degradation I (aerobic) | 1 | 1 | 1 |
| L-tyrosine biosynthesis IV | 1 | 1 | 1 |
| 3-dehydroquinate biosynthesis I | 2 | 2 | 1 |
| L-phenylalanine degradation V | 3 | 3 | 1 |
| chorismate biosynthesis I | 7 | 7 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| superpathway of L-tryptophan biosynthesis | 13 | 13 | 1 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 1 |
| superpathway of chorismate metabolism | 59 | 42 | 1 |