Experiment set47S209 for Pseudomonas putida KT2440

mixed carbon sources Trisodium citrate dihydrate 10 mM and L-Phenylalanine 5 mM

Group: mixed carbon source
Media: MME_noCarbon + Trisodium citrate dihydrate (10 mM) + L-Phenylalanine (5 mM)
Culturing: Putida_ML5_PNNL, 96 deep well, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 11/17/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 5 genes in this experiment

For mixed carbon source Trisodium citrate dihydrate in Pseudomonas putida KT2440

For mixed carbon source Trisodium citrate dihydrate across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tyrosine metabolism
• Glutathione metabolism
• Styrene degradation
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
gentisate degradation I	3	1	1
4-hydroxy-2-nonenal detoxification	4	1	1
L-tyrosine degradation I	5	5	1
pentachlorophenol degradation	10	4	2
5-nitroanthranilate degradation	6	2	1
glutathione-mediated detoxification I	8	3	1
gliotoxin biosynthesis	9	2	1
glutathione-mediated detoxification II	9	1	1
3-phenylpropanoate degradation	10	4	1
indole glucosinolate activation (intact plant cell)	12	3	1
camalexin biosynthesis	12	2	1