Experiment set46S478 for Pseudomonas putida KT2440
microaerobic M9
Group: microaerobic m9Media: M9_2percentGlucose_100mMammonium, pH=7
Culturing: Putida_ML5_JBEI, flask, Aerobic, at 30 (C), shaken=100 rpm
By: Aparajitha (Abhi) Srinivasan on 09/02/23
Media components: 20 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 100 mM Ammonium chloride
Specific Phenotypes
For 1 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Thiamin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glyphosate degradation II | 1 | 1 | 1 |
| thiazole component of thiamine diphosphate biosynthesis II | 7 | 4 | 1 |
| butanol and isobutanol biosynthesis (engineered) | 8 | 3 | 1 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 8 | 1 |