Experiment set46IT024 for Escherichia coli BW25113

Compare to:

Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; 0.02X nutrient broth; outgrowth in LBwithKan

200 most detrimental genes:

  gene name fitness t score description  
b4313 fimE +1.9 13.3 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1361 ydaW +1.4 1.2 orf, hypothetical protein (VIMSS) compare
b1685 ydiH +1.4 3.4 predicted protein (RefSeq) compare
b3863 polA +1.2 2.2 DNA polymerase I (NCBI) compare
b4503 yafF +1.2 2.2 no description compare
b1882 cheY +1.0 1.1 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1728 ydjM +1.0 2.8 predicted inner membrane protein regulated by LexA (RefSeq) compare
b2096 gatY +0.7 3.0 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1465 narV +0.6 2.1 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1462 b1462 +0.6 2.6 orf, hypothetical protein (VIMSS) compare
b1553 ydfP +0.6 1.8 Qin prophage; conserved protein (NCBI) compare
b2531 iscR +0.6 1.0 DNA-binding transcriptional repressor (NCBI) compare
b1070 flgN +0.6 1.3 export chaperone for FlgK and FlgL (NCBI) compare
b0696 kdpC +0.6 3.2 potassium-transporting ATPase subunit C (NCBI) compare
b0968 yccX +0.6 1.9 predicted acylphosphatase (NCBI) compare
b4647 mokA +0.6 0.7 no description compare
b1171 b1171 +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b4535 yniD +0.6 1.2 predicted protein (RefSeq) compare
b1648 b1648 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b4505 insX +0.5 1.2 predicted IS protein (RefSeq) compare
b2953 yggU +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b1758 b1758 +0.5 1.1 putative cytochrome oxidase (VIMSS) compare
b1010 ycdK +0.5 3.3 hypothetical protein (NCBI) compare
b1134 ymfB +0.5 2.1 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b2080 b2080 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b1382 ynbE +0.5 1.2 predicted lipoprotein (NCBI) compare
b1387 maoC +0.5 3.0 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1085 b1085 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b0937 ssuE +0.5 2.3 NAD(P)H-dependent FMN reductase (NCBI) compare
b1778 yeaA +0.5 1.5 methionine sulfoxide reductase B (NCBI) compare
b0746 valZ +0.5 1.8 tRNA-Val (NCBI) compare
b4144 yjeI +0.5 3.2 orf, hypothetical protein (VIMSS) compare
b1865 nudB +0.5 1.3 dATP pyrophosphohydrolase (NCBI) compare
b2093 gatB +0.5 2.7 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2058 wcaB +0.5 1.0 predicted acyl transferase (NCBI) compare
b2434 b2434 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b2212 alkB +0.5 2.4 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b3950 frwB +0.5 2.2 predicted enzyme IIB component of PTS (NCBI) compare
b1760 ynjH +0.5 1.7 hypothetical protein (NCBI) compare
b0580 ybdJ +0.5 1.1 predicted inner membrane protein (NCBI) compare
b2582 trxC +0.5 2.1 thioredoxin 2 (NCBI) compare
b2911 ssrS +0.5 1.4 6S regulatory RNA (NCBI) compare
b1671 ydhX +0.5 1.7 predicted 4Fe-4S ferridoxin-type protein (RefSeq) compare
b4394 yjjX +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.5 1.0 hypothetical protein (NCBI) compare
b1841 yobA +0.5 1.4 hypothetical protein (NCBI) compare
b1418 cybB +0.5 1.4 cytochrome b(561) (VIMSS) compare
b1321 ycjX +0.5 2.6 conserved protein with nucleoside triphosphate hydrolase domain (NCBI) compare
b4614 sokA +0.5 1.5 no description compare
b1242 ychE +0.5 2.0 predicted inner membrane protein (NCBI) compare
b1400 paaY +0.5 1.9 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b4513 kdpF +0.5 1.9 potassium ion accessory transporter subunit (NCBI) compare
b2309 hisJ +0.5 2.7 histidine/lysine/arginine/ornithine transporter subunit (NCBI) compare
b4675 yoaJ +0.5 1.4 expressed protein (RefSeq) compare
b2934 cmtB +0.4 0.6 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1591 b1591 +0.4 0.9 putative oxidoreductase component (VIMSS) compare
b2007 yeeX +0.4 1.3 conserved protein (RefSeq) compare
b4246 pyrL +0.4 1.4 pyrBI operon leader peptide (NCBI) compare
b1669 ydhT +0.4 1.0 hypothetical protein (NCBI) compare
b1278 pgpB +0.4 1.5 phosphatidylglycerophosphatase B (NCBI) compare
b0191 yaeJ +0.4 2.6 hypothetical protein (NCBI) compare
b4408 csrB +0.4 2.4 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b1640 ydhH +0.4 2.0 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b2409 yfeR +0.4 2.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1150 ymfR +0.4 0.7 e14 prophage; predicted protein (NCBI) compare
b4436 ryeC +0.4 0.8 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1904 yecR +0.4 2.1 hypothetical protein (NCBI) compare
b2005 yeeV +0.4 1.8 CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b2003 yeeT +0.4 1.1 CP4-44 prophage; predicted protein (NCBI) compare
b4493 gapC +0.4 1.0 no description compare
b4406 yaeP +0.4 1.2 hypothetical protein (NCBI) compare
b1231 tyrT +0.4 0.7 tRNA-Tyr (NCBI) compare
b0352 mhpE +0.4 2.3 4-hydroxy-2-ketovalerate aldolase (NCBI) compare
b1299 puuR +0.4 2.5 DNA-binding transcriptional repressor (NCBI) compare
b0841 ybjG +0.4 2.0 undecaprenyl pyrophosphate phosphatase (NCBI) compare
b4653 yicT +0.4 1.4 no description compare
b4330 yjiH +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1295 ymjA +0.4 1.8 hypothetical protein (NCBI) compare
b1566 flxA +0.4 1.9 Qin prophage; predicted protein (NCBI) compare
b0998 torD +0.4 0.7 chaperone protein TorD (NCBI) compare
b1185 dsbB +0.4 1.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1488 ddpX +0.4 1.8 D-ala-D-ala dipeptidase, Zn-dependent (NCBI) compare
b4480 hdfR +0.4 2.3 transcriptional regulator HdfR (NCBI) compare
b1075 flgD +0.4 2.1 flagellar basal body rod modification protein D (NCBI) compare
b0995 torR +0.4 2.0 DNA-binding response regulator in two-component regulatory system with TorS (NCBI) compare
b4702 mgtL +0.4 0.5 regulatory leader peptide for mgtA (NCBI) compare
b2886 ygfS +0.4 2.3 putative oxidoreductase, Fe-S subunit (VIMSS) compare
b2832 ygdQ +0.4 1.8 predicted inner membrane protein (NCBI) compare
b2079 baeR +0.4 2.8 DNA-binding response regulator in two-component regulatory system with BaeS (NCBI) compare
b3354 yheU +0.4 2.4 hypothetical protein (NCBI) compare
b4381 deoC +0.4 1.8 deoxyribose-phosphate aldolase (NCBI) compare
b1667 ydhR +0.4 1.6 hypothetical protein (NCBI) compare
b1593 b1593 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b4511 ybdZ +0.4 2.2 hypothetical protein (NCBI) compare
b1573 ydfC +0.4 0.6 Qin prophage; predicted protein (RefSeq) compare
b4596 yciZ +0.4 1.0 hypothetical protein (RefSeq) compare
b2186 yejK +0.4 2.9 nucleoid-associated protein NdpA (NCBI) compare
b4445 omrB +0.4 1.5 sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq) compare
b1380 ldhA +0.4 0.8 D-lactate dehydrogenase (NCBI) compare
b1560 ydfU +0.4 1.9 Qin prophage; predicted protein (RefSeq) compare
b2726 hypA +0.4 2.5 protein involved in nickel insertion into hydrogenases 3 (NCBI) compare
b0957 ompA +0.4 1.6 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0975 hyaD +0.3 1.5 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b1699 ydiS +0.3 1.5 predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI) compare
b3860 dsbA +0.3 2.7 periplasmic protein disulfide isomerase I (NCBI) compare
b1673 ydhV +0.3 1.8 predicted oxidoreductase (NCBI) compare
b3676 yidH +0.3 1.5 conserved inner membrane protein (NCBI) compare
b1883 cheB +0.3 1.3 chemotaxis-specific methylesterase (NCBI) compare
b1074 flgC +0.3 1.0 flagellar basal-body rod protein C (NCBI) compare
b1901 araF +0.3 2.0 L-arabinose transporter subunit (NCBI) compare
b1441 ydcT +0.3 1.5 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1945 fliM +0.3 2.5 flagellar motor switch protein M (NCBI) compare
b2829 ptsP +0.3 3.0 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1890 motA +0.3 1.5 flagellar motor protein MotA (NCBI) compare
b2426 ucpA +0.3 1.4 putative oxidoreductase (VIMSS) compare
b1296 ycjJ +0.3 1.9 putative amino acid/amine transport protein (VIMSS) compare
b3280 yrdB +0.3 1.2 hypothetical protein (NCBI) compare
b1383 ydbL +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1562 hokD +0.3 1.2 Qin prophage; small toxic polypeptide (NCBI) compare
b0880 cspD +0.3 1.1 cold shock protein homolog (NCBI) compare
b4363 yjjB +0.3 1.5 conserved inner membrane protein (RefSeq) compare
b1153 ymfQ +0.3 1.8 e14 prophage; conserved protein (NCBI) compare
b1286 rnb +0.3 1.2 exoribonuclease II (NCBI) compare
b2432 yfeY +0.3 1.2 hypothetical protein (NCBI) compare
b1818 manY +0.3 1.2 mannose-specific enzyme IIC component of PTS (NCBI) compare
b2381 ypdB +0.3 2.3 predicted response regulator in two-component system withYpdA (NCBI) compare
b1939 fliG +0.3 1.6 flagellar motor switch protein G (NCBI) compare
b2011 sbcB +0.3 2.3 exonuclease I (NCBI) compare
b1282 yciH +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b3868 glnG +0.3 2.2 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b1749 xthA +0.3 1.6 exonuclease III (NCBI) compare
b4093 phnO +0.3 2.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1217 chaB +0.3 0.6 cation transport regulator (NCBI) compare
b1620 malI +0.3 0.9 DNA-binding transcriptional repressor (NCBI) compare
b4585 sroB +0.3 0.6 Novel sRNA, function unknown (RefSeq) compare
b1767 ansA +0.3 2.1 cytoplasmic asparaginase I (NCBI) compare
b1135 ymfC +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b4360 yjjA +0.3 1.9 putative glycoprotein/receptor (VIMSS) compare
b1622 malY +0.3 1.7 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare
b1601 ydgG +0.3 1.7 predicted inner membrane protein (NCBI) compare
b1270 btuR +0.3 1.2 cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI) compare
b0927 ycbL +0.3 1.8 predicted metal-binding enzyme (NCBI) compare
b2094 gatA +0.3 1.6 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b2060 b2060 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b0226 dinJ +0.3 1.3 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1960 vsr +0.3 1.3 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b0232 yafN +0.3 0.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1248 b1248 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1511 ydeV +0.3 1.6 putative kinase (VIMSS) compare
b2420 yfeS +0.3 1.3 hypothetical protein (NCBI) compare
b1036 ycdZ +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1847 yebF +0.3 0.8 predicted protein (RefSeq) compare
b1279 yciS +0.3 0.5 conserved inner membrane protein (NCBI) compare
b2421 cysM +0.3 1.7 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b1302 puuE +0.3 1.9 GABA aminotransferase, PLP-dependent (NCBI) compare
b0596 entA +0.3 1.7 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI) compare
b2506 yfgI +0.3 1.0 hypothetical protein (NCBI) compare
b0225 yafQ +0.3 0.9 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3029 ygiN +0.3 0.9 quinol monooxygenase (NCBI) compare
b1819 manZ +0.3 1.3 mannose-specific enzyme IID component of PTS (NCBI) compare
b1415 aldA +0.3 2.1 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b2102 yegX +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b0606 ahpF +0.3 2.1 alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS) compare
b2066 udk +0.3 1.7 uridine/cytidine kinase (VIMSS) compare
b0092 ddlB +0.3 1.3 D-alanylalanine synthetase (NCBI) compare
b1301 puuB +0.3 1.4 gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI) compare
b1358 ydaT +0.3 0.6 Rac prophage; predicted protein (NCBI) compare
b0822 ybiV +0.3 1.7 predicted hydrolase (NCBI) compare
b1526 yneJ +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b3129 sohA +0.3 1.4 predicted regulator (NCBI) compare
b3074 ygjH +0.3 1.6 hypothetical protein (NCBI) compare
b1946 fliN +0.3 1.7 flagellar motor switch protein (NCBI) compare
b1930 yedF +0.3 1.9 hypothetical protein (NCBI) compare
b1523 yneG +0.3 0.7 hypothetical protein (NCBI) compare
b1606 folM +0.3 2.0 short chain dehydrogenase (NCBI) compare
b1512 lsrR +0.3 1.7 lsr operon transcriptional repressor (NCBI) compare
b1147 ymfL +0.3 0.7 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b0249 ykfF +0.3 1.2 CP4-6 prophage; predicted protein (NCBI) compare
b2804 fucU +0.3 0.8 L-fucose mutarotase (NCBI) compare
b0013 yaaI +0.3 1.4 hypothetical protein (NCBI) compare
b4701 sokX +0.3 0.4 sok-related sRNA, function unknown (RefSeq) compare
b1013 ycdC +0.3 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b4332 yjiJ +0.3 2.1 predicted inner membrane protein (NCBI) compare
b2771 ygcS +0.3 1.3 putative transport protein (VIMSS) compare
b1610 tus +0.3 1.8 DNA replication terminus site-binding protein (NCBI) compare
b1259 yciG +0.3 0.6 orf, hypothetical protein (VIMSS) compare
b3106 yhaK +0.3 1.4 predicted pirin-related protein (NCBI) compare
b2266 elaB +0.3 1.5 hypothetical protein (NCBI) compare
b0864 artP +0.3 1.5 arginine transporter subunit (NCBI) compare
b1081 flgJ +0.3 1.4 flagellar biosynthesis protein FlgJ (NCBI) compare
b2237 inaA +0.3 0.8 hypothetical protein (NCBI) compare
b3443 yrhA +0.3 0.4 orf, hypothetical protein (VIMSS) compare
b2377 yfdY +0.3 1.0 predicted inner membrane protein (NCBI) compare
b3228 sspB +0.3 1.6 ClpXP protease specificity-enhancing factor (NCBI) compare
b3999 yjaG +0.3 1.9 hypothetical protein (NCBI) compare
b4649 ysaD +0.3 1.6 no description compare
b0555 ybcS +0.3 1.8 DLP12 prophage; predicted lysozyme (NCBI) compare
b4110 yjcZ +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b1199 dhaL +0.3 1.5 dihydroxyacetone kinase, C-terminal domain (NCBI) compare
b1556 b1556 +0.3 0.8 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in mixed community experiments

For mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:100 starting OD to Ecoli; outgrowth in LBwithKan across organisms