Experiment set45S707 for Pseudomonas putida KT2440

Time6-FRS

Group: phagefrs
Media: MM2_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Putida_ML5a, 25mL flask, Aerobic, at 28.5 (C), shaken=orbital, continuous, 165cpm
By: Kate on 8/16/2023

Specific Phenotypes

For 4 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Folate Biosynthesis	1
One-carbon metabolism by tetrahydropterines	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism
• Cyanoamino acid metabolism
• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate
• Folate biosynthesis
• Nitrogen metabolism
• Biosynthesis of phenylpropanoids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-asparagine degradation I	1	1	1
L-glutamine degradation I	1	1	1
4-aminobenzoate biosynthesis I	2	2	1
L-glutamate biosynthesis I	2	2	1
ammonia assimilation cycle III	3	3	1
L-asparagine degradation III (mammalian)	3	2	1
formaldehyde oxidation VII (THF pathway)	4	4	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	3	1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation	5	2	1
L-glutamate and L-glutamine biosynthesis	7	6	1
L-citrulline biosynthesis	8	7	1
chloramphenicol biosynthesis	9	1	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
superpathway of candicidin biosynthesis	11	4	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
superpathway of L-citrulline metabolism	12	9	1
folate transformations I	13	9	1
purine nucleobases degradation I (anaerobic)	15	6	1
purine nucleobases degradation II (anaerobic)	24	16	1
superpathway of chorismate metabolism	59	42	1