Experiment set42IT067 for Escherichia coli BW25113

Compare to:

LB + carbenicillin regrowth after 48hr

200 most detrimental genes:

  gene name fitness t score description  
b1185 dsbB +2.7 12.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA +2.0 18.7 periplasmic protein disulfide isomerase I (NCBI) compare
b1361 ydaW +1.4 1.2 orf, hypothetical protein (VIMSS) compare
b2156 lysP +1.3 11.4 lysine transporter (NCBI) compare
b1831 proQ +1.1 4.1 putative solute/DNA competence effector (NCBI) compare
b4704 arrS +1.0 0.5 Antisense sRNA ArrS, function unknown (NCBI) compare
b0730 mngR +0.9 2.1 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4676 yoaK +0.9 0.7 expressed protein (RefSeq) compare
b4642 yoeD +0.9 1.8 no description compare
b1195 ymgE +0.9 3.3 predicted inner membrane protein (NCBI) compare
b1238 tdk +0.8 5.6 thymidine kinase (NCBI) compare
b3009 yghB +0.8 5.7 conserved inner membrane protein (NCBI) compare
b0464 acrR +0.8 6.9 DNA-binding transcriptional repressor (NCBI) compare
b0630 lipB +0.8 1.4 protein of lipoate biosynthesis (VIMSS) compare
b3181 greA +0.7 4.9 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1530 marR +0.7 2.6 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0929 ompF +0.7 4.2 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b4596 yciZ +0.7 1.4 hypothetical protein (RefSeq) compare
b0542 renD +0.7 2.6 orf, hypothetical protein (VIMSS) compare
b1476 fdnI +0.6 1.1 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1936 intG +0.6 0.9 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1954 dsrA +0.6 2.7 regulatory antisense RNA (NCBI) compare
b4699 fnrS +0.5 0.9 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3961 oxyR +0.5 4.8 DNA-binding transcriptional dual regulator (NCBI) compare
b3889 yiiE +0.5 2.2 predicted transcriptional regulator (RefSeq) compare
b1506 yneL +0.5 0.9 predicted transcriptional regulator (NCBI) compare
b1566 flxA +0.5 2.6 Qin prophage; predicted protein (NCBI) compare
b2209 eco +0.5 1.4 ecotin precursor (NCBI) compare
b2916 argP +0.5 3.6 chromosome replication initiation inhibitor protein (NCBI) compare
b1739 osmE +0.5 2.2 DNA-binding transcriptional activator (NCBI) compare
b0564 appY +0.5 1.2 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0191 yaeJ +0.5 3.6 hypothetical protein (NCBI) compare
b4538 yoeF +0.5 1.4 hypothetical protein (RefSeq) compare
b1919 yedO +0.5 1.9 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1841 yobA +0.4 1.6 hypothetical protein (NCBI) compare
b1179 ycgL +0.4 2.3 conserved protein (NCBI) compare
b4157 yjeN +0.4 1.4 hypothetical protein (NCBI) compare
b4241 treR +0.4 4.0 trehalose repressor (NCBI) compare
b2934 cmtB +0.4 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b4387 ytjB +0.4 3.0 hypothetical protein (NCBI) compare
b1753 ynjA +0.4 1.7 hypothetical protein (NCBI) compare
b0613 citG +0.4 2.1 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b1600 mdtJ +0.4 1.3 multidrug efflux system transporter (NCBI) compare
b4520 ymgF +0.4 1.3 hypothetical protein (NCBI) compare
b1305 pspB +0.4 1.5 phage shock protein B (NCBI) compare
b4573 insZ +0.4 2.1 no description compare
b1524 yneH +0.4 0.8 predicted glutaminase (NCBI) compare
b4042 dgkA +0.4 0.3 diacylglycerol kinase (NCBI) compare
b0848 ybjM +0.4 2.5 predicted inner membrane protein (NCBI) compare
b4603 rseX +0.4 0.5 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2096 gatY +0.4 2.7 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b3396 mrcA +0.4 3.7 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b1038 csgF +0.4 2.0 predicted transport protein (NCBI) compare
b4089 rpiR +0.4 3.5 transcriptional repressor of rpiB expression (VIMSS) compare
b1590 ynfH +0.4 1.8 oxidoreductase, membrane subunit (NCBI) compare
b1233 ychJ +0.4 2.5 hypothetical protein (NCBI) compare
b0843 ybjH +0.4 1.0 hypothetical protein (NCBI) compare
b1205 ychH +0.4 1.3 predicted inner membrane protein (NCBI) compare
b1930 yedF +0.4 2.7 hypothetical protein (NCBI) compare
b2193 narP +0.4 2.3 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b2409 yfeR +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b4063 soxR +0.3 1.9 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b1977 asnT +0.3 1.3 tRNA-Asn (NCBI) compare
b4471 tdcG +0.3 3.2 L-serine dehydratase 3 (NCBI) compare
b4558 yifL +0.3 2.1 predicted lipoprotein (NCBI) compare
b1618 uidR +0.3 2.0 DNA-binding transcriptional repressor (NCBI) compare
b1044 ymdA +0.3 2.2 hypothetical protein (NCBI) compare
b3147 yraM +0.3 3.0 hypothetical protein (NCBI) compare
b2264 menD +0.3 2.9 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b2996 hybA +0.3 2.8 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) compare
b1742 b1742 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2899 yqfA +0.3 2.2 predicted oxidoreductase, inner membrane subunit (NCBI) compare
b0798 ybiA +0.3 1.0 hypothetical protein (NCBI) compare
b2195 ccmG +0.3 1.2 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b0933 ssuB +0.3 1.7 alkanesulfonate transporter subunit (NCBI) compare
b2014 yeeF +0.3 2.3 putative amino acid/amine transport protein (VIMSS) compare
b0853 ybjN +0.3 1.8 predicted oxidoreductase (NCBI) compare
b1823 cspC +0.3 1.4 stress protein, member of the CspA-family (NCBI) compare
b3844 fre +0.3 1.9 NAD(P)H-flavin reductase (NCBI) compare
b0521 ybcF +0.3 2.0 predicted carbamate kinase (NCBI) compare
b2445 yffN +0.3 1.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b2263 yfbB +0.3 1.3 predicted peptidase (NCBI) compare
b1144 ymfJ +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1287 yciW +0.3 1.4 putative oxidoreductase (VIMSS) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b2792 yqcC +0.3 1.2 hypothetical protein (NCBI) compare
b2998 yghW +0.3 1.7 hypothetical protein (NCBI) compare
b3068 ygjF +0.3 1.9 G/U mismatch-specific DNA glycosylase (NCBI) compare
b2853 ygeI +0.3 0.2 hypothetical protein (NCBI) compare
b0456 ybaA +0.3 1.4 hypothetical protein (NCBI) compare
b1573 ydfC +0.3 0.6 Qin prophage; predicted protein (RefSeq) compare
b1070 flgN +0.3 0.8 export chaperone for FlgK and FlgL (NCBI) compare
b4444 rygA +0.3 1.3 unknown RNA (NCBI) compare
b4215 ytfI +0.3 1.0 hypothetical protein (NCBI) compare
b1438 ydcQ +0.3 1.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0418 pgpA +0.3 1.8 phosphatidylglycerophosphatase A (NCBI) compare
b4505 insX +0.3 0.9 predicted IS protein (RefSeq) compare
b2859 ygeO +0.3 0.8 hypothetical protein (RefSeq) compare
b2155 cirA +0.3 2.1 ferric iron-catecholate outer membrane transporter (NCBI) compare
b4104 phnE +0.3 1.4 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b1419 ydcA +0.3 2.1 hypothetical protein (NCBI) compare
b4313 fimE +0.3 2.3 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2749 ygbE +0.3 2.1 conserved inner membrane protein (NCBI) compare
b2260 menE +0.3 2.4 O-succinylbenzoic acid--CoA ligase (NCBI) compare
b3623 rfaK +0.3 1.0 lipopolysaccharide core biosynthesis (NCBI) compare
b0802 ybiJ +0.3 1.5 hypothetical protein (NCBI) compare
b0713 ybgL +0.3 2.1 hypothetical protein (NCBI) compare
b4367 fhuF +0.3 2.3 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b3043 ygiL +0.3 1.8 predicted fimbrial-like adhesin protein (NCBI) compare
b2261 menC +0.3 1.7 O-succinylbenzoate synthase (NCBI) compare
b1902 yecI +0.3 1.7 predicted ferritin-like protein (NCBI) compare
b1686 ydiI +0.3 0.5 hypothetical protein (NCBI) compare
b1892 flhD +0.3 1.8 DNA-binding transcriptional dual regulator with FlhC (RefSeq) compare
b2684 mprA +0.3 1.6 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1081 flgJ +0.3 2.0 flagellar biosynthesis protein FlgJ (NCBI) compare
b4640 yoeG +0.3 1.0 no description compare
b1756 ynjD +0.3 1.8 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2208 napF +0.3 1.6 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b4443 gcvB +0.3 0.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b4481 rffT +0.3 1.7 4-alpha-L-fucosyltransferase (NCBI) compare
b3627 rfaI +0.3 2.2 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1678 ynhG +0.3 2.1 hypothetical protein (NCBI) compare
b4646 yrdE +0.3 1.0 no description compare
b2741 rpoS +0.3 1.8 RNA polymerase sigma factor (NCBI) compare
b1838 pphA +0.3 1.9 protein phosphatase 1 modulates phosphoproteins, signals protein misfolding (VIMSS) compare
b1875 yecM +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0296 ykgM +0.3 1.2 50S ribosomal protein L31 (NCBI) compare
b4125 dcuS +0.3 2.3 sensory histidine kinase in two-component regulatory system with DcuR, regulator of anaerobic fumarate respiration (NCBI) compare
b2809 b2809 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1661 cfa +0.3 1.9 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b1478 adhP +0.3 1.4 alcohol dehydrogenase (VIMSS) compare
b1805 fadD +0.3 2.1 acyl-CoA synthase (NCBI) compare
b3790 rffC +0.3 1.5 TDP-fucosamine acetyltransferase (RefSeq) compare
b3861 yihF +0.3 2.1 putative GTP-binding protein (VIMSS) compare
b2587 kgtP +0.3 1.5 alpha-ketoglutarate transporter (NCBI) compare
b2997 hybO +0.3 2.0 hydrogenase 2, small subunit (NCBI) compare
b3646 yicG +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b0209 yafD +0.3 1.9 hypothetical protein (NCBI) compare
b3628 rfaB +0.2 2.2 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b2765 ygcM +0.2 1.2 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b2187 yejL +0.2 1.3 hypothetical protein (NCBI) compare
b2180 yejF +0.2 2.0 fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI) compare
b1357 ydaS +0.2 0.8 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1348 lar +0.2 0.6 Rac prophage; restriction alleviation protein (NCBI) compare
b3516 gadX +0.2 2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b0192 nlpE +0.2 1.6 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b3875 ompL +0.2 1.1 predicted outer membrane porin L (NCBI) compare
b4376 osmY +0.2 1.4 periplasmic protein (NCBI) compare
b0447 ybaO +0.2 1.9 putative LRP-like transcriptional regulator (VIMSS) compare
b1387 maoC +0.2 1.4 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1462 b1462 +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b3512 GadE +0.2 1.5 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b2535 csiE +0.2 2.2 orf, hypothetical protein (VIMSS) compare
b0611 rna +0.2 1.4 ribonuclease I (NCBI) compare
b0393 rdgC +0.2 2.0 recombination associated protein (NCBI) compare
b1227 narI +0.2 1.2 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b2993 hybD +0.2 1.4 predicted maturation element for hydrogenase 2 (NCBI) compare
b0193 yaeF +0.2 1.9 orf, hypothetical protein (VIMSS) compare
b3191 yrbB +0.2 2.0 orf, hypothetical protein (VIMSS) compare
b0998 torD +0.2 0.5 chaperone protein TorD (NCBI) compare
b3794 rffM +0.2 1.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b2177 yejA +0.2 2.0 orf, hypothetical protein (VIMSS) compare
b4277 yjgZ +0.2 1.6 KpLE2 phage-like element; predicted protein (NCBI) compare
b1730 ydjO +0.2 0.5 predicted protein (RefSeq) compare
b1811 yoaH +0.2 1.1 hypothetical protein (NCBI) compare
b2252 ais +0.2 1.4 hypothetical protein (NCBI) compare
b1000 cbpA +0.2 1.4 curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI) compare
b1152 ymfP +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b2182 bcr +0.2 1.4 bicyclomycin/multidrug efflux system (NCBI) compare
b1822 rrmA +0.2 0.7 23S rRNA m1G745 methyltransferase (NCBI) compare
b0804 ybiX +0.2 1.4 putative enzyme (VIMSS) compare
b2832 ygdQ +0.2 1.7 predicted inner membrane protein (NCBI) compare
b0678 nagB +0.2 1.2 glucosamine-6-phosphate deaminase (NCBI) compare
b2214 yojL +0.2 1.8 predicted thiamine biosynthesis lipoprotein (NCBI) compare
b0122 yacC +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b2051 wcaH +0.2 0.8 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0015 dnaJ +0.2 1.7 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1408 b1408 +0.2 1.4 probable enzyme (VIMSS) compare
b2783 chpR +0.2 0.6 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b2481 hyfA +0.2 0.9 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1251 yciI +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b2685 emrA +0.2 1.7 multidrug efflux system (NCBI) compare
b4613 dinQ +0.2 0.9 Damage inducible, function unknown (RefSeq) compare
b2010 dacD +0.2 1.4 penicillin binding protein 6b (VIMSS) compare
b0696 kdpC +0.2 0.8 potassium-transporting ATPase subunit C (NCBI) compare
b2518 ndk +0.2 0.6 nucleoside diphosphate kinase (NCBI) compare
b2618 b2618 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b2500 purN +0.2 1.4 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2804 fucU +0.2 1.2 L-fucose mutarotase (NCBI) compare
b1948 fliP +0.2 1.5 flagellar biosynthesis protein P (NCBI) compare
b2444 yffM +0.2 0.8 CPZ-55 prophage; predicted protein (NCBI) compare
b3723 bglG +0.2 1.4 transcriptional antiterminator of the bgl operon (NCBI) compare
b2217 rcsB +0.2 1.5 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1733 chbG +0.2 1.2 hypothetical protein (NCBI) compare
b3234 degQ +0.2 1.8 serine endoprotease, periplasmic (NCBI) compare
b3037 ygiB +0.2 1.3 conserved outer membrane protein (RefSeq) compare
b3476 nikA +0.2 1.8 nickel transporter subunit (NCBI) compare
b1625 ydgT +0.2 1.4 predicted regulator (NCBI) compare


Specific Phenotypes

For 14 genes in this experiment

For stress Carbenicillin disodium salt in Escherichia coli BW25113

For stress Carbenicillin disodium salt across organisms