Experiment set42IT063 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time9hr

200 most detrimental genes:

  gene name fitness t score description  
b2934 cmtB +1.3 1.1 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b3860 dsbA +1.2 10.3 periplasmic protein disulfide isomerase I (NCBI) compare
b2699 recA +1.1 1.8 recombinase A (NCBI) compare
b1185 dsbB +1.1 8.3 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1130 phoP +0.8 1.8 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b0798 ybiA +0.7 2.6 hypothetical protein (NCBI) compare
b2156 lysP +0.7 6.3 lysine transporter (NCBI) compare
b2071 yegJ +0.7 2.6 hypothetical protein (NCBI) compare
b1476 fdnI +0.7 1.2 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1831 proQ +0.6 2.6 putative solute/DNA competence effector (NCBI) compare
b3790 rffC +0.6 3.9 TDP-fucosamine acetyltransferase (RefSeq) compare
b4481 rffT +0.6 4.2 4-alpha-L-fucosyltransferase (NCBI) compare
b0730 mngR +0.6 1.3 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3794 rffM +0.6 4.1 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0802 ybiJ +0.6 2.6 hypothetical protein (NCBI) compare
b3786 rffE +0.6 5.0 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3787 rffD +0.6 4.8 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2209 eco +0.6 1.4 ecotin precursor (NCBI) compare
b4642 yoeD +0.5 1.6 no description compare
b2859 ygeO +0.5 1.9 hypothetical protein (RefSeq) compare
b1195 ymgE +0.5 2.2 predicted inner membrane protein (NCBI) compare
b4596 yciZ +0.5 1.1 hypothetical protein (RefSeq) compare
b3021 ygiT +0.5 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3784 rfe +0.5 4.5 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1830 prc +0.5 3.5 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3396 mrcA +0.5 4.4 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b1954 dsrA +0.5 2.1 regulatory antisense RNA (NCBI) compare
b1753 ynjA +0.5 1.9 hypothetical protein (NCBI) compare
b0933 ssuB +0.4 2.4 alkanesulfonate transporter subunit (NCBI) compare
b1882 cheY +0.4 0.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4157 yjeN +0.4 1.3 hypothetical protein (NCBI) compare
b3961 oxyR +0.4 3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b3791 rffA +0.4 3.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4480 hdfR +0.4 2.9 transcriptional regulator HdfR (NCBI) compare
b1765 ydjA +0.4 0.7 predicted oxidoreductase (NCBI) compare
b2510 b2510 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b0183 rnhB +0.4 2.5 ribonuclease HII (NCBI) compare
b0218 yafU +0.4 1.5 predicted inner membrane protein (RefSeq) compare
b4546 ypeB +0.4 0.9 hypothetical protein (NCBI) compare
b2187 yejL +0.4 1.8 hypothetical protein (NCBI) compare
b2051 wcaH +0.4 1.1 GDP-mannose mannosyl hydrolase (VIMSS) compare
b1418 cybB +0.4 1.5 cytochrome b(561) (VIMSS) compare
b1348 lar +0.4 1.2 Rac prophage; restriction alleviation protein (NCBI) compare
b1357 ydaS +0.4 1.2 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1332 ynaJ +0.4 0.2 predicted inner membrane protein (NCBI) compare
b1085 b1085 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1742 b1742 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b1160 elbA +0.4 0.7 hypothetical protein (NCBI) compare
b3512 GadE +0.4 2.2 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b4376 osmY +0.4 2.4 periplasmic protein (NCBI) compare
b1233 ychJ +0.4 2.5 hypothetical protein (NCBI) compare
b0114 aceE +0.4 0.9 pyruvate dehydrogenase subunit E1 (NCBI) compare
b1919 yedO +0.4 1.5 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b4505 insX +0.4 1.1 predicted IS protein (RefSeq) compare
b4538 yoeF +0.4 1.1 hypothetical protein (RefSeq) compare
b4699 fnrS +0.4 0.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b4603 rseX +0.4 0.5 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1299 puuR +0.4 2.1 DNA-binding transcriptional repressor (NCBI) compare
b4573 insZ +0.4 1.7 no description compare
b1686 ydiI +0.4 0.9 hypothetical protein (NCBI) compare
b4698 mgrR +0.4 2.1 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1709 btuD +0.3 0.6 vitamin B12-transporter ATPase (NCBI) compare
b2741 rpoS +0.3 2.8 RNA polymerase sigma factor (NCBI) compare
b3147 yraM +0.3 3.0 hypothetical protein (NCBI) compare
b2261 menC +0.3 2.3 O-succinylbenzoate synthase (NCBI) compare
b3250 mreC +0.3 1.2 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b3622 rfaL +0.3 1.9 O-antigen ligase (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b1625 ydgT +0.3 2.2 predicted regulator (NCBI) compare
b4000 hupA +0.3 2.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0521 ybcF +0.3 2.0 predicted carbamate kinase (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b1724 ydiZ +0.3 1.3 hypothetical protein (NCBI) compare
b2014 yeeF +0.3 2.3 putative amino acid/amine transport protein (VIMSS) compare
b1977 asnT +0.3 1.6 tRNA-Asn (NCBI) compare
b4609 ryfD +0.3 1.6 Novel sRNA, function unknown (RefSeq) compare
b4570 lomR +0.3 1.4 no description compare
b4660 yhiL +0.3 0.2 no description compare
b4369 leuP +0.3 1.7 tRNA-Leu (NCBI) compare
b1733 chbG +0.3 1.5 hypothetical protein (NCBI) compare
b1591 b1591 +0.3 0.9 putative oxidoreductase component (VIMSS) compare
b2476 purC +0.3 0.9 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1557 cspB +0.3 0.9 Qin prophage; cold shock protein (NCBI) compare
b1138 ymfE +0.3 0.9 e14 prophage; predicted inner membrane protein (NCBI) compare
b1789 yeaL +0.3 1.6 conserved inner membrane protein (NCBI) compare
b2481 hyfA +0.3 2.0 hydrogenase 4 Fe-S subunit (VIMSS) compare
b4381 deoC +0.3 1.9 deoxyribose-phosphate aldolase (NCBI) compare
b3844 fre +0.3 1.9 NAD(P)H-flavin reductase (NCBI) compare
b1930 yedF +0.3 2.4 hypothetical protein (NCBI) compare
b2208 napF +0.3 1.7 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b2792 yqcC +0.3 1.2 hypothetical protein (NCBI) compare
b2250 b2250 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1515 lsrD +0.3 1.5 AI2 transporter (NCBI) compare
b0641 rlpB +0.3 1.3 minor lipoprotein (NCBI) compare
b1129 phoQ +0.3 0.8 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b4441 glmY +0.3 1.0 sRNA activator of glmS mRNA (RefSeq) compare
b1287 yciW +0.3 1.5 putative oxidoreductase (VIMSS) compare
b1305 pspB +0.3 1.0 phage shock protein B (NCBI) compare
b4558 yifL +0.3 2.3 predicted lipoprotein (NCBI) compare
b4431 rprA +0.3 1.1 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b0393 rdgC +0.3 2.2 recombination associated protein (NCBI) compare
b2532 yfhQ +0.3 1.9 predicted methyltransferase (NCBI) compare
b3623 rfaK +0.3 1.0 lipopolysaccharide core biosynthesis (NCBI) compare
b1276 acnA +0.3 1.7 aconitate hydratase (NCBI) compare
b2202 napC +0.3 1.3 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b0191 yaeJ +0.3 2.0 hypothetical protein (NCBI) compare
b4640 yoeG +0.3 1.0 no description compare
b4045 yjbJ +0.3 1.3 predicted stress response protein (NCBI) compare
b1485 ddpC +0.3 1.5 D-ala-D-ala transporter subunit (NCBI) compare
b1961 dcm +0.3 0.7 DNA cytosine methylase (NCBI) compare
b1238 tdk +0.3 1.6 thymidine kinase (NCBI) compare
b2916 argP +0.3 1.7 chromosome replication initiation inhibitor protein (NCBI) compare
b0212 gloB +0.3 1.5 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b1792 yeaO +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1344 ydaO +0.3 1.1 predicted C32 tRNA thiolase (NCBI) compare
b4360 yjjA +0.3 1.4 putative glycoprotein/receptor (VIMSS) compare
b0713 ybgL +0.3 1.9 hypothetical protein (NCBI) compare
b2263 yfbB +0.3 0.9 predicted peptidase (NCBI) compare
b1179 ycgL +0.3 1.1 conserved protein (NCBI) compare
b1143 ymfI +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b1035 ycdY +0.3 1.8 hypothetical protein (NCBI) compare
b4513 kdpF +0.3 0.8 potassium ion accessory transporter subunit (NCBI) compare
b2782 chpA +0.3 1.8 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b0803 ybiI +0.3 1.4 hypothetical protein (NCBI) compare
b4687 shoB +0.3 1.6 toxic membrane protein (RefSeq) compare
b0101 yacG +0.3 1.3 zinc-binding protein (NCBI) compare
b4367 fhuF +0.3 2.1 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b3024 ygiW +0.3 1.1 hypothetical protein (NCBI) compare
b4184 yjfL +0.3 1.5 conserved inner membrane protein (NCBI) compare
b1252 tonB +0.3 1.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1950 fliR +0.3 1.2 flagellar biosynthesis protein R (NCBI) compare
b1800 yeaU +0.3 2.0 predicted dehydrogenase (NCBI) compare
b2486 hyfF +0.3 2.1 NADH dehydrogenase subunit N (NCBI) compare
b4410 ecnA +0.3 0.7 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b4692 oweE +0.3 0.8 no description compare
b2010 dacD +0.2 1.9 penicillin binding protein 6b (VIMSS) compare
b2897 ygfY +0.2 1.2 hypothetical protein (NCBI) compare
b3391 hofQ +0.2 2.3 predicted fimbrial transporter (NCBI) compare
b3074 ygjH +0.2 1.8 hypothetical protein (NCBI) compare
b2504 yfgG +0.2 0.5 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b4388 serB +0.2 1.9 3-phosphoserine phosphatase (NCBI) compare
b4551 yheV +0.2 1.3 hypothetical protein (NCBI) compare
b0388 aroL +0.2 1.6 shikimate kinase II (NCBI) compare
b2399 yfeD +0.2 1.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0193 yaeF +0.2 1.9 orf, hypothetical protein (VIMSS) compare
b1566 flxA +0.2 1.3 Qin prophage; predicted protein (NCBI) compare
b1227 narI +0.2 1.5 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b3361 fic +0.2 1.8 stationary-phase protein, cell division (NCBI) compare
b4234 yjgA +0.2 1.1 hypothetical protein (NCBI) compare
b2356 yfdM +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b3392 yrfA +0.2 1.9 orf, hypothetical protein (VIMSS) compare
b1565 ydfV +0.2 1.1 Qin prophage; predicted protein (NCBI) compare
b0550 rusA +0.2 1.4 DLP12 prophage; endonuclease RUS (NCBI) compare
b4444 rygA +0.2 1.3 unknown RNA (NCBI) compare
b2488 hyfH +0.2 1.1 hydrogenase 4, Fe-S subunit (NCBI) compare
b2169 fruB +0.2 1.7 fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI) compare
b1502 ydeQ +0.2 0.9 predicted fimbrial-like adhesin protein (NCBI) compare
b2900 yqfB +0.2 1.5 hypothetical protein (NCBI) compare
b2535 csiE +0.2 2.2 orf, hypothetical protein (VIMSS) compare
b1114 mfd +0.2 1.8 transcription-repair coupling factor (NCBI) compare
b1610 tus +0.2 1.4 DNA replication terminus site-binding protein (NCBI) compare
b1045 ymdB +0.2 1.6 hypothetical protein (NCBI) compare
b4508 peaD +0.2 1.3 putative sensory transduction regulator (VIMSS) compare
b1070 flgN +0.2 0.7 export chaperone for FlgK and FlgL (NCBI) compare
b0862 artQ +0.2 1.1 arginine transporter subunit (NCBI) compare
b1907 tyrP +0.2 1.3 tyrosine transporter (NCBI) compare
b1410 ynbC +0.2 1.6 predicted hydrolase (NCBI) compare
b1823 cspC +0.2 1.0 stress protein, member of the CspA-family (NCBI) compare
b1936 intG +0.2 0.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4374 yjjG +0.2 1.9 nucleotidase (NCBI) compare
b4380 yjjI +0.2 1.9 hypothetical protein (NCBI) compare
b2682 ygaZ +0.2 1.4 predicted transporter (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b0518 fdrA +0.2 1.7 membrane protein FdrA (NCBI) compare
b2264 menD +0.2 1.9 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b0714 nei +0.2 1.3 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b1038 csgF +0.2 0.9 predicted transport protein (NCBI) compare
b2408 yfeN +0.2 1.7 conserved outer membrane protein (NCBI) compare
b0535 fimZ +0.2 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b4396 rob +0.2 2.0 DNA-binding transcriptional activator (NCBI) compare
b0323 yahI +0.2 1.7 predicted carbamate kinase-like protein (NCBI) compare
b4543 ypaA +0.2 1.1 predicted protein (NCBI) compare
b0736 ybgC +0.2 0.8 predicted acyl-CoA thioesterase (NCBI) compare
b1768 ydjB +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b4519 icdC +0.2 1.1 no description compare
b2577 yfiE +0.2 1.3 putative transcriptional regulator LYSR-type (VIMSS) compare
b2221 atoD +0.2 1.1 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b3480 nikE +0.2 1.8 nickel transporter subunit (NCBI) compare
b2291 yfbR +0.2 1.2 hypothetical protein (NCBI) compare
b1355 b1355 +0.2 0.8 orf, hypothetical protein (VIMSS) compare
b1058 yceO +0.2 0.1 hypothetical protein (NCBI) compare
b1547 stfQ +0.2 1.2 Qin prophage; predicted side tail fibre assembly protein (NCBI) compare
b4042 dgkA +0.2 0.1 diacylglycerol kinase (NCBI) compare
b0192 nlpE +0.2 1.7 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b0443 ybaW +0.2 1.5 hypothetical protein (NCBI) compare
b1220 ychO +0.2 1.2 predicted invasin (RefSeq) compare
b2959 yggL +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b1607 ydgC +0.2 0.8 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1875 yecM +0.2 1.2 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms