Experiment set42IT061 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time8hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -7.0 -14.2 alanine racemase (NCBI) compare
b0462 acrB -6.2 -29.0 multidrug efflux system protein (NCBI) compare
b0463 acrA -6.2 -16.4 multidrug efflux system (NCBI) compare
b4237 nrdG -5.4 -5.2 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.0 -10.1 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.6 -4.6 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4201 priB -2.7 -5.5 primosomal replication protein N (NCBI) compare
b2330 yfcB -2.6 -1.7 putative adenine-specific methylase (VIMSS) compare
b1860 ruvB -2.2 -1.5 Holliday junction DNA helicase B (NCBI) compare
b1334 fnr -2.1 -2.0 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b3035 tolC -2.1 -2.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4025 pgi -2.0 -11.0 glucose-6-phosphate isomerase (NCBI) compare
b3390 aroK -1.9 -3.8 shikimate kinase I (NCBI) compare
b1192 ldcA -1.9 -2.5 L,D-carboxypeptidase A (NCBI) compare
b0386 proC -1.8 -5.4 pyrroline-5-carboxylate reductase (NCBI) compare
b2620 smpB -1.8 -2.0 SsrA-binding protein (NCBI) compare
b0912 ihfB -1.8 -3.4 integration host factor subunit beta (NCBI) compare
b2830 nudH -1.7 -1.9 dinucleoside polyphosphate hydrolase (NCBI) compare
b1850 eda -1.5 -2.5 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2912 ygfA -1.5 -3.4 putative ligase (VIMSS) compare
b0243 proA -1.5 -9.0 gamma-glutamyl phosphate reductase (NCBI) compare
b0740 tolB -1.4 -3.2 translocation protein TolB precursor (NCBI) compare
b1861 ruvA -1.4 -1.4 Holliday junction DNA helicase motor protein (NCBI) compare
b3781 trxA -1.3 -5.2 thioredoxin 1 (VIMSS) compare
b0949 uup -1.3 -8.2 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1187 fadR -1.3 -2.7 fatty acid metabolism regulator (NCBI) compare
b0902 pflA -1.3 -5.0 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b4503 yafF -1.3 -1.4 no description compare
b4593 ymgI -1.3 -1.4 hypothetical protein (RefSeq) compare
b0990 cspG -1.2 -1.4 DNA-binding transcriptional regulator (NCBI) compare
b2820 recB -1.2 -0.8 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0149 mrcB -1.2 -9.3 penicillin-binding protein 1b (NCBI) compare
b1123 potD -1.2 -6.4 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b1863 ruvC -1.2 -1.6 Holliday junction resolvase (NCBI) compare
b3868 glnG -1.2 -6.9 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0242 proB -1.2 -5.5 gamma-glutamyl kinase (NCBI) compare
b0053 surA -1.1 -6.3 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0222 lpcA -1.1 -2.4 phosphoheptose isomerase (NCBI) compare
b2017 yefM -1.1 -0.9 orf, hypothetical protein (VIMSS) compare
b3183 obgE -1.1 -1.4 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1279 yciS -1.0 -1.8 conserved inner membrane protein (NCBI) compare
b3619 rfaD -1.0 -3.4 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1236 galU -1.0 -3.9 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1125 potB -1.0 -3.6 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0683 fur -1.0 -1.0 ferric uptake regulator (NCBI) compare
b1712 ihfA -0.9 -0.6 integration host factor subunit alpha (NCBI) compare
b0738 tolR -0.9 -1.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2297 pta -0.9 -0.6 phosphate acetyltransferase (NCBI) compare
b1677 lpp -0.9 -1.8 murein lipoprotein (NCBI) compare
b2699 recA -0.9 -0.6 recombinase A (NCBI) compare
b1292 sapC -0.9 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0303 ykgI -0.9 -0.8 orf, hypothetical protein (VIMSS) compare
b1124 potC -0.9 -4.2 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0014 dnaK -0.9 -2.4 molecular chaperone DnaK (NCBI) compare
b0728 sucC -0.9 -5.1 succinyl-CoA synthetase subunit beta (NCBI) compare
b1346 ydaQ -0.9 -1.5 orf, hypothetical protein (VIMSS) compare
b0903 pflB -0.9 -6.5 pyruvate formate lyase I (NCBI) compare
b3935 priA -0.8 -3.0 primosome assembly protein PriA (NCBI) compare
b1551 b1551 -0.8 -0.5 orf, hypothetical protein (VIMSS) compare
b0739 tolA -0.8 -1.9 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1175 minD -0.8 -4.0 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1286 rnb -0.8 -1.8 exoribonuclease II (NCBI) compare
b4202 rpsR -0.8 -1.6 30S ribosomal protein S18 (NCBI) compare
b2576 srmB -0.8 -2.8 ATP-dependent RNA helicase (NCBI) compare
b0234 yafP -0.8 -1.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3229 sspA -0.8 -2.0 stringent starvation protein A (NCBI) compare
b2329 aroC -0.8 -4.9 chorismate synthase (NCBI) compare
b0729 sucD -0.8 -3.7 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1273 yciN -0.8 -2.0 hypothetical protein (NCBI) compare
b3741 gidA -0.8 -2.3 glucose-inhibited division protein A (NCBI) compare
b3930 menA -0.8 -6.8 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1992 cobS -0.8 -2.1 cobalamin synthase (NCBI) compare
b3977 tyrU -0.8 -0.9 tRNA-Tyr (NCBI) compare
b0232 yafN -0.8 -1.0 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2343 b2343 -0.7 -2.4 orf, hypothetical protein (VIMSS) compare
b1293 sapB -0.7 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0564 appY -0.7 -1.4 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1347 ydaC -0.7 -0.4 Rac prophage; predicted protein (NCBI) compare
b0706 ybfD -0.7 -1.8 hypothetical protein (NCBI) compare
b3842 rfaH -0.7 -4.2 transcriptional activator RfaH (NCBI) compare
b2642 yfjW -0.7 -1.3 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b4402 yjjY -0.7 -0.8 hypothetical protein (NCBI) compare
b1171 b1171 -0.7 -1.5 orf, hypothetical protein (VIMSS) compare
b1804 rnd -0.7 -3.4 ribonuclease D (NCBI) compare
b1855 lpxM -0.7 -3.2 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1126 potA -0.7 -3.6 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b0118 acnB -0.7 -0.5 aconitate hydratase (NCBI) compare
b0167 glnD -0.7 -2.6 PII uridylyl-transferase (NCBI) compare
b2659 b2659 -0.7 -1.7 orf, hypothetical protein (VIMSS) compare
b2501 ppk -0.7 -5.1 polyphosphate kinase (NCBI) compare
b0953 rmf -0.7 -1.8 ribosome modulation factor (NCBI) compare
b2822 recC -0.7 -3.7 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b4177 purA -0.7 -1.5 adenylosuccinate synthetase (NCBI) compare
b1290 sapF -0.7 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2704 srlB -0.7 -4.0 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b3162 deaD -0.7 -2.8 inducible ATP-independent RNA helicase (VIMSS) compare
b0200 gmhB -0.6 -1.0 hypothetical protein (NCBI) compare
b1563 relE -0.6 -1.3 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b1147 ymfL -0.6 -2.3 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b3806 cyaA -0.6 -4.1 adenylate cyclase (NCBI) compare
b2625 yfjI -0.6 -1.3 CP4-57 prophage; predicted protein (NCBI) compare
b3493 pitA -0.6 -4.3 phosphate transporter, low-affinity (NCBI) compare
b3119 tdcR -0.6 -2.2 threonine dehydratase operon activator protein (VIMSS) compare
b0603 ybdO -0.6 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2024 hisA -0.6 -2.6 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2951 yggS -0.6 -3.0 predicted enzyme (NCBI) compare
b4255 yjgD -0.6 -3.0 hypothetical protein (NCBI) compare
b0908 aroA -0.6 -4.2 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b0438 clpX -0.6 -3.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1306 pspC -0.6 -2.5 DNA-binding transcriptional activator (NCBI) compare
b2894 xerD -0.6 -0.5 site-specific tyrosine recombinase XerD (NCBI) compare
b1253 yciA -0.6 -3.1 predicted hydrolase (NCBI) compare
b4701 sokX -0.6 -0.8 sok-related sRNA, function unknown (RefSeq) compare
b4436 ryeC -0.6 -1.5 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b4655 ythA -0.6 -0.9 expressed protein (RefSeq) compare
b4413 sokC -0.6 -1.6 regulatory antisense RNA (NCBI) compare
b3792 wzxE -0.6 -3.8 O-antigen translocase (NCBI) compare
b4294 insA-7 -0.6 -1.9 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b1595 ynfL -0.6 -1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b4191 ulaR -0.5 -2.7 DNA-binding transcriptional dual regulator (NCBI) compare
b1266 yciV -0.5 -2.9 hypothetical protein (NCBI) compare
b1862 yebB -0.5 -2.6 orf, hypothetical protein (VIMSS) compare
b1235 rssB -0.5 -3.6 response regulator of RpoS (NCBI) compare
b1818 manY -0.5 -2.2 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1258 yciF -0.5 -0.6 hypothetical protein (NCBI) compare
b0737 tolQ -0.5 -1.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0357 yaiN -0.5 -1.5 putative alpha helix chain (VIMSS) compare
b0500 ybbD -0.5 -0.3 hypothetical protein (RefSeq) compare
b1176 minC -0.5 -2.0 septum formation inhibitor (NCBI) compare
b1048 mdoG -0.5 -3.1 glucan biosynthesis protein, periplasmic (NCBI) compare
b2567 rnc -0.5 -0.3 ribonuclease III (NCBI) compare
b4179 vacB -0.5 -3.4 putative enzyme (VIMSS) compare
b2531 iscR -0.5 -0.9 DNA-binding transcriptional repressor (NCBI) compare
b2553 glnB -0.5 -2.3 regulatory protein P-II for glutamine synthetase (NCBI) compare
b3706 trmE -0.5 -2.1 tRNA modification GTPase (NCBI) compare
b4462 ygaQ -0.5 -1.3 hypothetical protein (RefSeq) compare
b2215 ompC -0.5 -3.8 outer membrane porin protein C (NCBI) compare
b3630 rfaP -0.5 -3.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1105 ycfM -0.5 -1.6 predicted outer membrane lipoprotein (NCBI) compare
b0685 ybfE -0.5 -1.7 orf, hypothetical protein (VIMSS) compare
b3115 tdcD -0.5 -4.5 propionate kinase/acetate kinase C, anaerobic (RefSeq) compare
b1497 b1497 -0.5 -0.3 putative enzyme (VIMSS) compare
b2814 metZ -0.5 -1.2 tRNA-Met (NCBI) compare
b1648 b1648 -0.5 -1.3 orf, hypothetical protein (VIMSS) compare
b4048 yjbM -0.5 -1.0 hypothetical protein (NCBI) compare
b2299 yfcD -0.5 -2.6 predicted NUDIX hydrolase (NCBI) compare
b2313 cvpA -0.5 -2.7 membrane protein required for colicin V production (NCBI) compare
b4497 yeeL -0.5 -0.7 no description compare
b1493 gadB -0.5 -0.8 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1865 nudB -0.5 -0.8 dATP pyrophosphohydrolase (NCBI) compare
b2055 wcaE -0.5 -0.2 predicted glycosyl transferase (NCBI) compare
b3620 rfaF -0.5 -2.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3636 rpmG -0.5 -1.3 50S ribosomal protein L33 (NCBI) compare
b3609 secB -0.5 -2.5 export protein SecB (NCBI) compare
b3052 rfaE -0.5 -2.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1522 yneF -0.5 -2.5 predicted diguanylate cyclase (NCBI) compare
b4545 ypdJ -0.5 -1.7 no description compare
b0437 clpP -0.5 -2.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0145 dksA -0.5 -1.6 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3405 ompR -0.5 -2.0 osmolarity response regulator (NCBI) compare
b0741 pal -0.5 -1.5 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1628 rsxB -0.4 -1.8 electron transport complex protein RnfB (NCBI) compare
b0688 pgm -0.4 -1.6 phosphoglucomutase (NCBI) compare
b4375 prfC -0.4 -1.4 peptide chain release factor 3 (NCBI) compare
b1593 b1593 -0.4 -2.3 orf, hypothetical protein (VIMSS) compare
b1827 kdgR -0.4 -2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1728 ydjM -0.4 -1.4 predicted inner membrane protein regulated by LexA (RefSeq) compare
b4523 yciX -0.4 -2.5 hypothetical protein (RefSeq) compare
b2007 yeeX -0.4 -2.0 conserved protein (RefSeq) compare
b3631 rfaG -0.4 -3.9 glucosyltransferase I (NCBI) compare
b3290 trkA -0.4 -0.5 potassium transporter peripheral membrane component (NCBI) compare
b1001 yccE -0.4 -0.2 hypothetical protein (NCBI) compare
b3387 dam -0.4 -3.4 DNA adenine methylase (NCBI) compare
b3621 rfaC -0.4 -1.9 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0237 pepD -0.4 -3.3 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b1617 uidA -0.4 -2.8 beta-D-glucuronidase (NCBI) compare
b2318 truA -0.4 -0.9 tRNA pseudouridine synthase A (NCBI) compare
b0049 apaH -0.4 -1.0 diadenosinetetraphosphatase (NCBI) compare
b1291 sapD -0.4 -0.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0998 torD -0.4 -0.8 chaperone protein TorD (NCBI) compare
b1049 mdoH -0.4 -3.0 glucosyltransferase MdoH (NCBI) compare
b3465 yhhF -0.4 -2.8 predicted methyltransferase (NCBI) compare
b0082 mraW -0.4 -2.0 S-adenosyl-methyltransferase (NCBI) compare
b0890 ftsK -0.4 -1.5 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0957 ompA -0.4 -2.0 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b1594 dgsA -0.4 -1.7 DNA-binding transcriptional repressor (NCBI) compare
b0705 ybfL -0.4 -0.3 putative receptor protein (VIMSS) compare
b2540 hcaC -0.4 -1.7 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI) compare
b1148 ymfM -0.4 -1.8 e14 prophage; predicted protein (NCBI) compare
b1984 asnW -0.4 -0.8 tRNA-Asn (NCBI) compare
b1572 ydfB -0.4 -1.1 orf, hypothetical protein (VIMSS) compare
b2528 iscA -0.4 -0.7 iron-sulfur cluster assembly protein (NCBI) compare
b4702 mgtL -0.4 -0.9 regulatory leader peptide for mgtA (NCBI) compare
b2369 evgA -0.4 -1.8 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b1499 ydeO -0.4 -0.2 predicted DNA-binding transcriptional acfivator (NCBI) compare
b2352 yfdI -0.4 -0.5 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b4151 frdD -0.4 -1.3 fumarate reductase subunit D (NCBI) compare
b4435 isrC -0.4 -1.5 unknown RNA (NCBI) compare
b1550 b1550 -0.4 -0.8 orf, hypothetical protein (VIMSS) compare
b1516 lsrB -0.4 -1.2 AI2 transporter (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms