Experiment set42IT061 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time8hr

200 most detrimental genes:

  gene name fitness t score description  
b1185 dsbB +1.0 5.6 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4538 yoeF +1.0 3.3 hypothetical protein (RefSeq) compare
b2934 cmtB +1.0 0.8 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1831 proQ +0.9 4.8 putative solute/DNA competence effector (NCBI) compare
b4603 rseX +0.9 1.2 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b2846 yqeH +0.8 0.6 orf, hypothetical protein (VIMSS) compare
b3860 dsbA +0.8 7.0 periplasmic protein disulfide isomerase I (NCBI) compare
b0798 ybiA +0.8 2.4 hypothetical protein (NCBI) compare
b0542 renD +0.8 2.5 orf, hypothetical protein (VIMSS) compare
b0730 mngR +0.7 1.6 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1332 ynaJ +0.7 0.8 predicted inner membrane protein (NCBI) compare
b1724 ydiZ +0.7 1.6 hypothetical protein (NCBI) compare
b2504 yfgG +0.7 1.2 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1823 cspC +0.7 2.7 stress protein, member of the CspA-family (NCBI) compare
b2616 recN +0.7 5.3 recombination and repair protein (NCBI) compare
b1205 ychH +0.6 2.1 predicted inner membrane protein (NCBI) compare
b0880 cspD +0.6 2.5 cold shock protein homolog (NCBI) compare
b1936 intG +0.6 1.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1882 cheY +0.6 0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1085 b1085 +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b1480 sra +0.5 1.4 30S ribosomal subunit protein S22 (NCBI) compare
b1765 ydjA +0.5 0.9 predicted oxidoreductase (NCBI) compare
b0617 citD +0.5 2.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1753 ynjA +0.5 1.6 hypothetical protein (NCBI) compare
b1476 fdnI +0.5 0.8 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1195 ymgE +0.5 1.9 predicted inner membrane protein (NCBI) compare
b0802 ybiJ +0.5 1.9 hypothetical protein (NCBI) compare
b1434 ydcN +0.5 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2156 lysP +0.5 4.2 lysine transporter (NCBI) compare
b4480 hdfR +0.5 2.9 transcriptional regulator HdfR (NCBI) compare
b2684 mprA +0.4 2.6 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0623 cspE +0.4 1.7 cold shock protein E (NCBI) compare
b1669 ydhT +0.4 2.0 hypothetical protein (NCBI) compare
b4505 insX +0.4 0.8 predicted IS protein (RefSeq) compare
b0191 yaeJ +0.4 2.6 hypothetical protein (NCBI) compare
b4596 yciZ +0.4 1.3 hypothetical protein (RefSeq) compare
b3181 greA +0.4 2.7 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1448 yncA +0.4 1.5 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4609 ryfD +0.4 2.3 Novel sRNA, function unknown (RefSeq) compare
b3764 yifE +0.4 3.0 hypothetical protein (NCBI) compare
b1954 dsrA +0.4 1.8 regulatory antisense RNA (NCBI) compare
b4699 fnrS +0.4 0.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b4646 yrdE +0.4 2.1 no description compare
b2741 rpoS +0.4 2.8 RNA polymerase sigma factor (NCBI) compare
b0521 ybcF +0.4 1.9 predicted carbamate kinase (NCBI) compare
b4653 yicT +0.4 1.5 no description compare
b3206 npr +0.4 3.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4045 yjbJ +0.4 1.6 predicted stress response protein (NCBI) compare
b1610 tus +0.4 2.4 DNA replication terminus site-binding protein (NCBI) compare
b3100 yqjK +0.4 1.8 hypothetical protein (NCBI) compare
b1790 yeaM +0.4 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1874 cutC +0.4 0.9 copper homeostasis protein (NCBI) compare
b1930 yedF +0.4 2.4 hypothetical protein (NCBI) compare
b3512 GadE +0.4 2.2 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1503 ydeR +0.4 1.6 predicted fimbrial-like adhesin protein (NCBI) compare
b1848 yebG +0.4 1.0 conserved protein regulated by LexA (NCBI) compare
b0630 lipB +0.4 0.6 protein of lipoate biosynthesis (VIMSS) compare
b2804 fucU +0.4 1.4 L-fucose mutarotase (NCBI) compare
b2915 yqfE +0.4 1.6 hypothetical protein (RefSeq) compare
b0596 entA +0.4 2.6 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI) compare
b4541 yehK +0.4 1.6 hypothetical protein (NCBI) compare
b1824 yobF +0.4 1.1 hypothetical protein (NCBI) compare
b4686 yshB +0.4 1.5 expressed protein (RefSeq) compare
b1422 ydcI +0.4 2.8 putative transcriptional regulator LYSR-type (VIMSS) compare
b1081 flgJ +0.4 2.2 flagellar biosynthesis protein FlgJ (NCBI) compare
b1305 pspB +0.3 1.4 phage shock protein B (NCBI) compare
b0550 rusA +0.3 1.9 DLP12 prophage; endonuclease RUS (NCBI) compare
b1414 ydcF +0.3 2.1 hypothetical protein (NCBI) compare
b2217 rcsB +0.3 2.8 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1530 marR +0.3 1.5 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1955 yedP +0.3 1.8 hypothetical protein (NCBI) compare
b1418 cybB +0.3 1.6 cytochrome b(561) (VIMSS) compare
b0183 rnhB +0.3 1.6 ribonuclease HII (NCBI) compare
b4551 yheV +0.3 1.7 hypothetical protein (NCBI) compare
b3627 rfaI +0.3 2.8 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2287 nuoB +0.3 1.8 NADH dehydrogenase subunit B (NCBI) compare
b3961 oxyR +0.3 2.9 DNA-binding transcriptional dual regulator (NCBI) compare
b4363 yjjB +0.3 2.1 conserved inner membrane protein (RefSeq) compare
b2187 yejL +0.3 1.1 hypothetical protein (NCBI) compare
b3248 yhdE +0.3 2.7 Maf-like protein (NCBI) compare
b1387 maoC +0.3 1.7 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b2445 yffN +0.3 1.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b2051 wcaH +0.3 1.0 GDP-mannose mannosyl hydrolase (VIMSS) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b1689 b1689 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b0228 yafM +0.3 1.4 hypothetical protein (NCBI) compare
b1342 ydaN +0.3 2.0 zinc transporter (NCBI) compare
b3259 prmA +0.3 2.0 ribosomal protein L11 methyltransferase (NCBI) compare
b1446 ydcY +0.3 1.4 hypothetical protein (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b0836 yliH +0.3 1.1 hypothetical protein (NCBI) compare
b0713 ybgL +0.3 1.7 hypothetical protein (NCBI) compare
b3260 dusB +0.3 2.1 tRNA-dihydrouridine synthase B (NCBI) compare
b4376 osmY +0.3 1.3 periplasmic protein (NCBI) compare
b2990 hybG +0.3 1.1 hydrogenase 2 accessory protein (NCBI) compare
b2782 chpA +0.3 1.7 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b2209 eco +0.3 1.1 ecotin precursor (NCBI) compare
b4692 oweE +0.3 1.2 no description compare
b1520 b1520 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b3622 rfaL +0.3 1.5 O-antigen ligase (NCBI) compare
b0942 ycbU +0.3 1.9 predicted fimbrial-like adhesin protein (NCBI) compare
b1462 b1462 +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b1507 hipA +0.3 1.4 regulator with hipB (NCBI) compare
b2689 yqaA +0.3 1.8 conserved inner membrane protein (NCBI) compare
b0754 aroG +0.3 1.7 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b0456 ybaA +0.3 1.4 hypothetical protein (NCBI) compare
b1758 b1758 +0.3 0.7 putative cytochrome oxidase (VIMSS) compare
b3500 gor +0.3 0.7 glutathione reductase (NCBI) compare
b4144 yjeI +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2996 hybA +0.3 2.4 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) compare
b4418 sraB +0.3 0.7 unknown RNA (NCBI) compare
b1380 ldhA +0.3 1.3 D-lactate dehydrogenase (NCBI) compare
b1339 abgR +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1524 yneH +0.3 0.5 predicted glutaminase (NCBI) compare
b4492 ydbA +0.3 2.2 no description compare
b1904 yecR +0.3 1.5 hypothetical protein (NCBI) compare
b1360 ydaV +0.3 0.8 Rac prophage; predicted DNA replication protein (NCBI) compare
b2208 napF +0.3 1.5 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b2645 yfjZ +0.3 1.6 CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b4513 kdpF +0.3 0.9 potassium ion accessory transporter subunit (NCBI) compare
b4157 yjeN +0.3 0.9 hypothetical protein (NCBI) compare
b2010 dacD +0.3 2.1 penicillin binding protein 6b (VIMSS) compare
b4640 yoeG +0.3 0.9 no description compare
b2572 rseA +0.3 1.8 anti-sigma factor (NCBI) compare
b1415 aldA +0.3 2.0 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b0852 rimK +0.3 1.8 ribosomal protein S6 modification protein (NCBI) compare
b2587 kgtP +0.3 1.9 alpha-ketoglutarate transporter (NCBI) compare
b1744 astE +0.3 1.4 succinylglutamate desuccinylase (NCBI) compare
b2264 menD +0.3 2.2 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b2096 gatY +0.3 1.9 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b0192 nlpE +0.3 2.1 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b2510 b2510 +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b4515 ybgT +0.3 0.8 hypothetical protein (NCBI) compare
b0803 ybiI +0.3 1.0 hypothetical protein (NCBI) compare
b3226 yhcK +0.3 1.8 putative FADA-type transcriptional regulator (VIMSS) compare
b1613 manA +0.3 0.9 mannose-6-phosphate isomerase (VIMSS) compare
b0209 yafD +0.3 1.8 hypothetical protein (NCBI) compare
b2624 alpA +0.3 1.9 CP4-57 prophage; DNA-binding transcriptional activator (NCBI) compare
b2202 napC +0.3 1.5 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1337 abgB +0.3 1.5 predicted peptidase, aminobenzoyl-glutamate utilization protein (NCBI) compare
b4389 radA +0.3 2.1 predicted repair protein (NCBI) compare
b4650 yibS +0.3 1.1 no description compare
b1144 ymfJ +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b4234 yjgA +0.3 0.9 hypothetical protein (NCBI) compare
b1644 b1644 +0.3 1.6 putative membrane protein (VIMSS) compare
b1053 mdtG +0.3 1.3 predicted drug efflux system (NCBI) compare
b3481 nikR +0.2 2.0 nickel responsive regulator (NCBI) compare
b1359 ydaU +0.2 1.5 Rac prophage; conserved protein (NCBI) compare
b4387 ytjB +0.2 1.9 hypothetical protein (NCBI) compare
b1764 selD +0.2 1.4 selenophosphate synthetase (NCBI) compare
b0289 yagV +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b2016 yeeZ +0.2 1.6 predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b4558 yifL +0.2 1.9 predicted lipoprotein (NCBI) compare
b1348 lar +0.2 0.8 Rac prophage; restriction alleviation protein (NCBI) compare
b4561 insM +0.2 1.8 transposase (RefSeq) compare
b2165 yeiN +0.2 1.5 hypothetical protein (NCBI) compare
b1453 ansP +0.2 1.6 L-asparagine permease (VIMSS) compare
b1789 yeaL +0.2 1.1 conserved inner membrane protein (NCBI) compare
b3624 rfaZ +0.2 0.8 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1368 ynaA +0.2 0.9 Rac prophage; predicted tail protein (RefSeq) compare
b1421 trg +0.2 1.2 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1355 b1355 +0.2 0.7 orf, hypothetical protein (VIMSS) compare
b0632 dacA +0.2 1.8 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1164 ycgZ +0.2 0.7 hypothetical protein (NCBI) compare
b2022 hisB +0.2 1.5 imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS) compare
b1625 ydgT +0.2 1.5 predicted regulator (NCBI) compare
b4042 dgkA +0.2 0.1 diacylglycerol kinase (NCBI) compare
b2535 csiE +0.2 2.1 orf, hypothetical protein (VIMSS) compare
b4481 rffT +0.2 1.6 4-alpha-L-fucosyltransferase (NCBI) compare
b2580 ung +0.2 1.8 uracil-DNA glycosylase (NCBI) compare
b0548 ninE +0.2 1.4 DLP12 prophage; conserved protein (NCBI) compare
b0863 artI +0.2 1.4 arginine transporter subunit (NCBI) compare
b3198 kdsC +0.2 1.3 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2102 yegX +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b0545 ybcL +0.2 2.0 DLP12 prophage; predicted kinase inhibitor (NCBI) compare
b4592 yccB +0.2 0.8 hypothetical protein (RefSeq) compare
b4532 yncN +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b3625 rfaY +0.2 1.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0946 ycbW +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b3836 b3836 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b2004 yeeU +0.2 1.4 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b0589 fepG +0.2 1.7 iron-enterobactin transporter subunit (NCBI) compare
b3555 yiaG +0.2 1.1 predicted transcriptional regulator (NCBI) compare
b1737 chbC +0.2 1.5 N,N'-diacetylchitobiose-specific enzyme IIC component of PTS (NCBI) compare
b0731 mngA +0.2 1.7 fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI) compare
b0851 nfsA +0.2 1.3 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b4406 yaeP +0.2 0.8 hypothetical protein (NCBI) compare
b1478 adhP +0.2 1.3 alcohol dehydrogenase (VIMSS) compare
b3199 yrbK +0.2 0.8 hypothetical protein (NCBI) compare
b0819 ybiS +0.2 1.7 hypothetical protein (NCBI) compare
b2261 menC +0.2 1.7 O-succinylbenzoate synthase (NCBI) compare
b4241 treR +0.2 2.1 trehalose repressor (NCBI) compare
b4509 ylcG +0.2 0.7 DLP12 prophage; predicted protein (NCBI) compare
b2356 yfdM +0.2 0.7 orf, hypothetical protein (VIMSS) compare
b4658 yghX +0.2 1.6 no description compare
b2722 hycD +0.2 1.5 hydrogenase 3, membrane subunit (NCBI) compare
b2481 hyfA +0.2 1.5 hydrogenase 4 Fe-S subunit (VIMSS) compare
b4218 ytfL +0.2 1.7 predicted inner membrane protein (NCBI) compare
b1679 sufE +0.2 0.8 cysteine desufuration protein SufE (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms