Experiment set42IT055 for Escherichia coli BW25113

Compare to:

LB + carbenicillin regrowth after 48hr

200 most detrimental genes:

  gene name fitness t score description  
b1185 dsbB +2.6 12.1 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3979 thrT +2.1 0.8 tRNA-Thr (NCBI) compare
b3860 dsbA +2.0 18.2 periplasmic protein disulfide isomerase I (NCBI) compare
b1831 proQ +1.2 5.9 putative solute/DNA competence effector (NCBI) compare
b2156 lysP +1.2 10.6 lysine transporter (NCBI) compare
b0630 lipB +1.0 1.9 protein of lipoate biosynthesis (VIMSS) compare
b3181 greA +0.9 4.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1361 ydaW +0.9 0.7 orf, hypothetical protein (VIMSS) compare
b3009 yghB +0.9 6.9 conserved inner membrane protein (NCBI) compare
b0464 acrR +0.9 7.5 DNA-binding transcriptional repressor (NCBI) compare
b1238 tdk +0.8 5.2 thymidine kinase (NCBI) compare
b1765 ydjA +0.8 1.3 predicted oxidoreductase (NCBI) compare
b4596 yciZ +0.7 1.5 hypothetical protein (RefSeq) compare
b1954 dsrA +0.7 3.4 regulatory antisense RNA (NCBI) compare
b1530 marR +0.7 2.6 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0929 ompF +0.7 5.2 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b1480 sra +0.7 1.6 30S ribosomal subunit protein S22 (NCBI) compare
b0564 appY +0.6 1.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1434 ydcN +0.6 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b4699 fnrS +0.6 1.0 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1753 ynjA +0.6 1.8 hypothetical protein (NCBI) compare
b4505 insX +0.6 1.1 predicted IS protein (RefSeq) compare
b1476 fdnI +0.6 1.0 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1686 ydiI +0.6 2.0 hypothetical protein (NCBI) compare
b1936 intG +0.6 1.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0310 ykgH +0.6 1.4 predicted inner membrane protein (NCBI) compare
b0191 yaeJ +0.6 4.4 hypothetical protein (NCBI) compare
b2096 gatY +0.6 3.1 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1348 lar +0.5 1.8 Rac prophage; restriction alleviation protein (NCBI) compare
b4045 yjbJ +0.5 1.3 predicted stress response protein (NCBI) compare
b0730 mngR +0.5 1.1 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3026 qseC +0.5 2.1 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3396 mrcA +0.5 5.0 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b2934 cmtB +0.5 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b0683 fur +0.5 0.8 ferric uptake regulator (NCBI) compare
b1824 yobF +0.5 2.1 hypothetical protein (NCBI) compare
b1195 ymgE +0.5 1.4 predicted inner membrane protein (NCBI) compare
b1731 cedA +0.5 2.2 cell division modulator (RefSeq) compare
b4538 yoeF +0.5 1.3 hypothetical protein (RefSeq) compare
b3120 yhaB +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b2741 rpoS +0.5 3.2 RNA polymerase sigma factor (NCBI) compare
b0998 torD +0.4 1.0 chaperone protein TorD (NCBI) compare
b3512 GadE +0.4 2.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1742 b1742 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b4551 yheV +0.4 2.2 hypothetical protein (NCBI) compare
b2684 mprA +0.4 2.7 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0843 ybjH +0.4 1.7 hypothetical protein (NCBI) compare
b3961 oxyR +0.4 3.6 DNA-binding transcriptional dual regulator (NCBI) compare
b1739 osmE +0.4 2.1 DNA-binding transcriptional activator (NCBI) compare
b2916 argP +0.4 2.6 chromosome replication initiation inhibitor protein (NCBI) compare
b0802 ybiJ +0.4 1.4 hypothetical protein (NCBI) compare
b2264 menD +0.4 3.3 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b0209 yafD +0.4 3.3 hypothetical protein (NCBI) compare
b4618 tisB +0.4 2.2 lexA-regulated toxic peptide (RefSeq) compare
b4089 rpiR +0.4 3.8 transcriptional repressor of rpiB expression (VIMSS) compare
b0535 fimZ +0.4 2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3147 yraM +0.4 3.8 hypothetical protein (NCBI) compare
b1183 umuD +0.4 1.3 DNA polymerase V, subunit D (NCBI) compare
b3628 rfaB +0.4 3.6 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1233 ychJ +0.4 2.2 hypothetical protein (NCBI) compare
b4241 treR +0.4 3.6 trehalose repressor (NCBI) compare
b2261 menC +0.4 2.6 O-succinylbenzoate synthase (NCBI) compare
b1726 yniB +0.4 0.7 predicted inner membrane protein (NCBI) compare
b4515 ybgT +0.4 1.2 hypothetical protein (NCBI) compare
b0648 ybeU +0.4 2.7 predicted tRNA ligase (NCBI) compare
b1841 yobA +0.4 1.4 hypothetical protein (NCBI) compare
b0848 ybjM +0.4 2.1 predicted inner membrane protein (NCBI) compare
b0393 rdgC +0.4 3.0 recombination associated protein (NCBI) compare
b2535 csiE +0.4 3.1 orf, hypothetical protein (VIMSS) compare
b4367 fhuF +0.4 2.7 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b2217 rcsB +0.4 2.9 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1299 puuR +0.4 2.0 DNA-binding transcriptional repressor (NCBI) compare
b4063 soxR +0.4 2.0 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b1724 ydiZ +0.3 1.3 hypothetical protein (NCBI) compare
b4157 yjeN +0.3 1.0 hypothetical protein (NCBI) compare
b4573 insZ +0.3 1.7 no description compare
b4656 yjhY +0.3 2.0 no description compare
b3844 fre +0.3 2.5 NAD(P)H-flavin reductase (NCBI) compare
b4363 yjjB +0.3 1.9 conserved inner membrane protein (RefSeq) compare
b2618 b2618 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b4341 yjiS +0.3 2.5 hypothetical protein (NCBI) compare
b2250 b2250 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1143 ymfI +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1164 ycgZ +0.3 1.0 hypothetical protein (NCBI) compare
b1625 ydgT +0.3 2.3 predicted regulator (NCBI) compare
b1930 yedF +0.3 2.0 hypothetical protein (NCBI) compare
b4410 ecnA +0.3 0.9 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1847 yebF +0.3 0.6 predicted protein (RefSeq) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b2587 kgtP +0.3 2.4 alpha-ketoglutarate transporter (NCBI) compare
b0613 citG +0.3 1.4 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b0909 ycaL +0.3 1.9 putative heat shock protein (VIMSS) compare
b1448 yncA +0.3 1.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4381 deoC +0.3 2.2 deoxyribose-phosphate aldolase (NCBI) compare
b2700 ygaD +0.3 1.1 competence damage-inducible protein A (NCBI) compare
b1566 flxA +0.3 1.6 Qin prophage; predicted protein (NCBI) compare
b0183 rnhB +0.3 0.9 ribonuclease HII (NCBI) compare
b1070 flgN +0.3 0.8 export chaperone for FlgK and FlgL (NCBI) compare
b2208 napF +0.3 2.2 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b1558 cspF +0.3 1.3 Qin prophage; cold shock protein (NCBI) compare
b2209 eco +0.3 0.9 ecotin precursor (NCBI) compare
b3027 ygiZ +0.3 0.2 conserved inner membrane protein (NCBI) compare
b2804 fucU +0.3 1.5 L-fucose mutarotase (NCBI) compare
b2435 amiA +0.3 1.7 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b0521 ybcF +0.3 2.2 predicted carbamate kinase (NCBI) compare
b1342 ydaN +0.3 1.9 zinc transporter (NCBI) compare
b0542 renD +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b0418 pgpA +0.3 1.6 phosphatidylglycerophosphatase A (NCBI) compare
b4579 yaiX +0.3 1.9 no description compare
b2288 nuoA +0.3 1.1 NADH dehydrogenase I chain A (VIMSS) compare
b0294 ykgK +0.3 1.6 predicted regulator (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b0456 ybaA +0.3 1.6 hypothetical protein (NCBI) compare
b4642 yoeD +0.3 1.4 no description compare
b1427 rimL +0.3 1.2 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b2177 yejA +0.3 2.4 orf, hypothetical protein (VIMSS) compare
b4376 osmY +0.3 1.9 periplasmic protein (NCBI) compare
b1789 yeaL +0.3 1.4 conserved inner membrane protein (NCBI) compare
b1502 ydeQ +0.3 1.0 predicted fimbrial-like adhesin protein (NCBI) compare
b1800 yeaU +0.3 2.1 predicted dehydrogenase (NCBI) compare
b4457 csrC +0.3 1.4 regulatory RNA (NCBI) compare
b1000 cbpA +0.3 1.9 curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI) compare
b4660 yhiL +0.3 0.2 no description compare
b2010 dacD +0.3 2.1 penicillin binding protein 6b (VIMSS) compare
b3074 ygjH +0.3 1.8 hypothetical protein (NCBI) compare
b2749 ygbE +0.3 2.1 conserved inner membrane protein (NCBI) compare
b2195 ccmG +0.3 1.0 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b4032 malG +0.3 1.7 maltose transporter subunit (NCBI) compare
b2809 b2809 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b3725 pstB +0.3 0.1 phosphate transporter subunit (NCBI) compare
b4513 kdpF +0.3 0.8 potassium ion accessory transporter subunit (NCBI) compare
b1315 ycjS +0.3 2.0 predicted oxidoreductase, NADH-binding (NCBI) compare
b3236 mdh +0.3 2.1 malate dehydrogenase (NCBI) compare
b3516 gadX +0.3 2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b4676 yoaK +0.3 0.3 expressed protein (RefSeq) compare
b4484 cpxP +0.3 2.3 periplasmic protein combats stress (NCBI) compare
b2287 nuoB +0.3 1.3 NADH dehydrogenase subunit B (NCBI) compare
b3506 slp +0.3 2.4 outer membrane protein induced after carbon starvation (VIMSS) compare
b3624 rfaZ +0.3 1.0 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0245 ykfI +0.3 2.0 CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b2969 yghE +0.3 1.8 predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq) compare
b1151 ymfO +0.3 1.3 e14 prophage; conserved protein (RefSeq) compare
b4387 ytjB +0.3 1.6 hypothetical protein (NCBI) compare
b0973 hyaB +0.3 1.8 hydrogenase 1, large subunit (NCBI) compare
b0896 dmsC +0.3 2.1 dimethyl sulfoxide reductase, anaerobic, subunit C (NCBI) compare
b0934 ycbM +0.3 1.4 putative transport system permease protein (VIMSS) compare
b1573 ydfC +0.3 0.7 Qin prophage; predicted protein (RefSeq) compare
b4313 fimE +0.3 2.1 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b3723 bglG +0.3 1.7 transcriptional antiterminator of the bgl operon (NCBI) compare
b0928 aspC +0.3 1.7 aspartate aminotransferase, PLP-dependent (NCBI) compare
b4561 insM +0.3 1.6 transposase (RefSeq) compare
b4646 yrdE +0.3 1.0 no description compare
b2673 nrdH +0.3 1.5 glutaredoxin-like protein (NCBI) compare
b1287 yciW +0.3 1.1 putative oxidoreductase (VIMSS) compare
b3194 yrbE +0.2 2.1 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1344 ydaO +0.2 1.3 predicted C32 tRNA thiolase (NCBI) compare
b1661 cfa +0.2 1.6 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b3347 fkpA +0.2 2.3 FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI) compare
b1382 ynbE +0.2 0.7 predicted lipoprotein (NCBI) compare
b4471 tdcG +0.2 2.3 L-serine dehydratase 3 (NCBI) compare
b4441 glmY +0.2 0.8 sRNA activator of glmS mRNA (RefSeq) compare
b1227 narI +0.2 1.4 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b1083 flgL +0.2 1.2 flagellar hook-associated protein L (NCBI) compare
b0192 nlpE +0.2 1.7 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b4144 yjeI +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b2488 hyfH +0.2 1.2 hydrogenase 4, Fe-S subunit (NCBI) compare
b0193 yaeF +0.2 1.9 orf, hypothetical protein (VIMSS) compare
b2260 menE +0.2 2.1 O-succinylbenzoic acid--CoA ligase (NCBI) compare
b1013 ycdC +0.2 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2993 hybD +0.2 1.7 predicted maturation element for hydrogenase 2 (NCBI) compare
b1349 recT +0.2 1.8 Rac prophage; recombination and repair protein (NCBI) compare
b4345 mcrC +0.2 0.5 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b0713 ybgL +0.2 1.8 hypothetical protein (NCBI) compare
b0942 ycbU +0.2 1.7 predicted fimbrial-like adhesin protein (NCBI) compare
b2095 gatZ +0.2 1.7 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b0443 ybaW +0.2 1.6 hypothetical protein (NCBI) compare
b3507 dctR +0.2 2.2 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b1421 trg +0.2 1.4 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b0447 ybaO +0.2 1.8 putative LRP-like transcriptional regulator (VIMSS) compare
b2995 hybB +0.2 2.1 predicted hydrogenase 2 cytochrome b type component (NCBI) compare
b0933 ssuB +0.2 0.9 alkanesulfonate transporter subunit (NCBI) compare
b0996 torC +0.2 1.7 trimethylamine N-oxide (TMAO) reductase I, cytochrome c-type subunit (NCBI) compare
b3280 yrdB +0.2 1.1 hypothetical protein (NCBI) compare
b2180 yejF +0.2 2.0 fused predicted oligopeptide transporter subunits of ABC superfamilly: ATP-binding components (NCBI) compare
b0561 tfaD +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b0213 yafS +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b3191 yrbB +0.2 2.0 orf, hypothetical protein (VIMSS) compare
b4280 yjhC +0.2 1.8 putative dehydrogenase (VIMSS) compare
b1305 pspB +0.2 0.9 phage shock protein B (NCBI) compare
b1709 btuD +0.2 0.4 vitamin B12-transporter ATPase (NCBI) compare
b1220 ychO +0.2 1.6 predicted invasin (RefSeq) compare
b0617 citD +0.2 0.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b3622 rfaL +0.2 1.2 O-antigen ligase (NCBI) compare
b1805 fadD +0.2 1.8 acyl-CoA synthase (NCBI) compare
b2996 hybA +0.2 1.8 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) compare
b2014 yeeF +0.2 1.6 putative amino acid/amine transport protein (VIMSS) compare
b1604 ydgH +0.2 1.1 hypothetical protein (NCBI) compare
b2686 emrB +0.2 1.9 multidrug efflux system protein (NCBI) compare
b4458 oxyS +0.2 0.4 global regulatory RNA (NCBI) compare
b2019 hisG +0.2 1.4 ATP phosphoribosyltransferase (NCBI) compare


Specific Phenotypes

For 12 genes in this experiment

For stress Carbenicillin disodium salt in Escherichia coli BW25113

For stress Carbenicillin disodium salt across organisms