Experiment set42IT054 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time48hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -8.1 -11.0 alanine racemase (NCBI) compare
b4237 nrdG -5.8 -5.7 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.3 -10.7 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -5.1 -4.8 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -3.5 -13.6 glucose-6-phosphate isomerase (NCBI) compare
b1334 fnr -3.4 -3.2 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2620 smpB -2.9 -2.6 SsrA-binding protein (NCBI) compare
b4402 yjjY -2.8 -1.6 hypothetical protein (NCBI) compare
b1850 eda -2.8 -4.6 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0949 uup -2.4 -16.6 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0386 proC -2.4 -7.0 pyrroline-5-carboxylate reductase (NCBI) compare
b2330 yfcB -2.3 -2.1 putative adenine-specific methylase (VIMSS) compare
b1124 potC -2.0 -11.7 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1123 potD -1.9 -8.8 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b0243 proA -1.9 -12.6 gamma-glutamyl phosphate reductase (NCBI) compare
b0200 gmhB -1.9 -2.3 hypothetical protein (NCBI) compare
b1125 potB -1.8 -6.3 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0242 proB -1.8 -8.3 gamma-glutamyl kinase (NCBI) compare
b4375 prfC -1.8 -2.7 peptide chain release factor 3 (NCBI) compare
b0145 dksA -1.8 -5.1 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4676 yoaK -1.8 -1.0 expressed protein (RefSeq) compare
b1126 potA -1.7 -11.7 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b2912 ygfA -1.6 -3.9 putative ligase (VIMSS) compare
b1860 ruvB -1.6 -2.0 Holliday junction DNA helicase B (NCBI) compare
b0739 tolA -1.6 -3.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0688 pgm -1.6 -5.1 phosphoglucomutase (NCBI) compare
b2017 yefM -1.6 -1.4 orf, hypothetical protein (VIMSS) compare
b3035 tolC -1.5 -5.8 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0014 dnaK -1.5 -5.1 molecular chaperone DnaK (NCBI) compare
b3977 tyrU -1.4 -1.5 tRNA-Tyr (NCBI) compare
b3706 trmE -1.3 -4.4 tRNA modification GTPase (NCBI) compare
b3839 tatC -1.3 -6.8 TatABCE protein translocation system subunit (NCBI) compare
b0737 tolQ -1.3 -2.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0902 pflA -1.3 -5.9 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b2808 gcvA -1.3 -5.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3741 gidA -1.2 -3.3 glucose-inhibited division protein A (NCBI) compare
b3619 rfaD -1.2 -4.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0142 folK -1.2 -1.0 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3405 ompR -1.2 -5.0 osmolarity response regulator (NCBI) compare
b0741 pal -1.1 -3.5 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3868 glnG -1.1 -7.5 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b1551 b1551 -1.1 -0.7 orf, hypothetical protein (VIMSS) compare
b3842 rfaH -1.1 -6.2 transcriptional activator RfaH (NCBI) compare
b1677 lpp -1.1 -3.3 murein lipoprotein (NCBI) compare
b3162 deaD -1.1 -5.2 inducible ATP-independent RNA helicase (VIMSS) compare
b3781 trxA -1.1 -6.1 thioredoxin 1 (VIMSS) compare
b0053 surA -1.1 -6.0 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1730 ydjO -1.0 -1.7 predicted protein (RefSeq) compare
b2659 b2659 -1.0 -2.6 orf, hypothetical protein (VIMSS) compare
b4066 yjcF -1.0 -0.7 hypothetical protein (NCBI) compare
b3620 rfaF -1.0 -5.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1290 sapF -1.0 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3183 obgE -1.0 -1.5 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0728 sucC -1.0 -6.4 succinyl-CoA synthetase subunit beta (NCBI) compare
b4593 ymgI -1.0 -1.3 hypothetical protein (RefSeq) compare
b0303 ykgI -1.0 -1.0 orf, hypothetical protein (VIMSS) compare
b0903 pflB -1.0 -7.8 pyruvate formate lyase I (NCBI) compare
b0908 aroA -0.9 -6.7 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b2297 pta -0.9 -0.8 phosphate acetyltransferase (NCBI) compare
b2576 srmB -0.9 -3.6 ATP-dependent RNA helicase (NCBI) compare
b1048 mdoG -0.9 -4.9 glucan biosynthesis protein, periplasmic (NCBI) compare
b0167 glnD -0.9 -4.3 PII uridylyl-transferase (NCBI) compare
b0738 tolR -0.9 -1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2820 recB -0.9 -1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0222 lpcA -0.9 -2.5 phosphoheptose isomerase (NCBI) compare
b2329 aroC -0.9 -4.9 chorismate synthase (NCBI) compare
b4177 purA -0.9 -2.2 adenylosuccinate synthetase (NCBI) compare
b3500 gor -0.9 -2.2 glutathione reductase (NCBI) compare
b3389 aroB -0.9 -7.2 3-dehydroquinate synthase (NCBI) compare
b1049 mdoH -0.8 -6.8 glucosyltransferase MdoH (NCBI) compare
b0237 pepD -0.8 -6.0 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b4202 rpsR -0.8 -1.8 30S ribosomal protein S18 (NCBI) compare
b0149 mrcB -0.8 -7.2 penicillin-binding protein 1b (NCBI) compare
b2830 nudH -0.8 -1.4 dinucleoside polyphosphate hydrolase (NCBI) compare
b1171 b1171 -0.8 -3.6 orf, hypothetical protein (VIMSS) compare
b1192 ldcA -0.8 -1.7 L,D-carboxypeptidase A (NCBI) compare
b1861 ruvA -0.8 -1.1 Holliday junction DNA helicase motor protein (NCBI) compare
b3390 aroK -0.8 -2.0 shikimate kinase I (NCBI) compare
b3930 menA -0.8 -7.0 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b4685 yrbN -0.8 -2.8 expressed protein (RefSeq) compare
b0890 ftsK -0.8 -2.9 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b1628 rsxB -0.8 -4.3 electron transport complex protein RnfB (NCBI) compare
b0232 yafN -0.8 -1.0 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1863 ruvC -0.8 -1.4 Holliday junction resolvase (NCBI) compare
b3281 aroE -0.8 -6.9 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b4462 ygaQ -0.8 -2.0 hypothetical protein (RefSeq) compare
b1236 galU -0.8 -3.7 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1291 sapD -0.8 -1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3493 pitA -0.8 -5.3 phosphate transporter, low-affinity (NCBI) compare
b0603 ybdO -0.8 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3631 rfaG -0.8 -6.9 glucosyltransferase I (NCBI) compare
b4535 yniD -0.8 -1.1 predicted protein (RefSeq) compare
b4552 yrhC -0.8 -1.3 no description compare
b1286 rnb -0.8 -2.4 exoribonuclease II (NCBI) compare
b2318 truA -0.8 -2.8 tRNA pseudouridine synthase A (NCBI) compare
b2450 yffS -0.8 -2.1 CPZ-55 prophage; predicted protein (RefSeq) compare
b1593 b1593 -0.7 -3.6 orf, hypothetical protein (VIMSS) compare
b3916 pfkA -0.7 -2.5 6-phosphofructokinase (NCBI) compare
b4131 cadA -0.7 -6.8 lysine decarboxylase 1 (NCBI) compare
b3052 rfaE -0.7 -3.4 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0729 sucD -0.7 -3.5 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1159 mcrA -0.7 -1.4 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2501 ppk -0.7 -5.3 polyphosphate kinase (NCBI) compare
b1346 ydaQ -0.7 -1.3 orf, hypothetical protein (VIMSS) compare
b4647 mokA -0.7 -1.0 no description compare
b3495 uspA -0.7 -6.3 universal stress global response regulator (NCBI) compare
b3375 yhfR -0.7 -5.3 putative transcriptional regulator (VIMSS) compare
b2498 upp -0.7 -2.2 uracil phosphoribosyltransferase (VIMSS) compare
b1842 holE -0.7 -2.7 DNA polymerase III, theta subunit (NCBI) compare
b2393 nupC -0.7 -5.3 nucleoside (except guanosine) transporter (NCBI) compare
b3169 nusA -0.7 -2.2 transcription elongation factor NusA (NCBI) compare
b0437 clpP -0.7 -4.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1497 b1497 -0.7 -0.4 putative enzyme (VIMSS) compare
b3863 polA -0.7 -0.7 DNA polymerase I (NCBI) compare
b1001 yccE -0.7 -0.3 hypothetical protein (NCBI) compare
b2055 wcaE -0.7 -0.3 predicted glycosyl transferase (NCBI) compare
b1182 hlyE -0.6 -0.5 hemolysin E (VIMSS) compare
b1161 ycgX -0.6 -1.0 hypothetical protein (NCBI) compare
b0740 tolB -0.6 -2.0 translocation protein TolB precursor (NCBI) compare
b3911 cpxA -0.6 -4.8 two-component sensor protein (NCBI) compare
b2567 rnc -0.6 -0.3 ribonuclease III (NCBI) compare
b4132 cadB -0.6 -5.8 predicted lysine/cadaverine transporter (NCBI) compare
b1658 purR -0.6 -4.2 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b2569 lepA -0.6 -3.1 GTP-binding protein LepA (NCBI) compare
b0009 mog -0.6 -4.6 molybdenum cofactor biosynthesis protein (NCBI) compare
b1167 ymgC -0.6 -0.3 hypothetical protein (NCBI) compare
b1165 ymgA -0.6 -0.3 hypothetical protein (NCBI) compare
b0500 ybbD -0.6 -0.3 hypothetical protein (RefSeq) compare
b4151 frdD -0.6 -3.0 fumarate reductase subunit D (NCBI) compare
b1187 fadR -0.6 -2.8 fatty acid metabolism regulator (NCBI) compare
b1693 aroD -0.6 -3.4 3-dehydroquinate dehydratase (NCBI) compare
b1936 intG -0.6 -0.9 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2168 fruK -0.6 -1.0 1-phosphofructokinase (NCBI) compare
b1025 ycdT -0.6 -1.5 predicted diguanylate cyclase (NCBI) compare
b0658 ybeX -0.6 -0.6 predicteed ion transport (NCBI) compare
b1257 yciE -0.6 -0.3 hypothetical protein (NCBI) compare
b2652 ileY -0.6 -0.8 tRNA-Ile (NCBI) compare
b4384 deoD -0.6 -4.6 purine nucleoside phosphorylase (NCBI) compare
b2531 iscR -0.6 -1.7 DNA-binding transcriptional repressor (NCBI) compare
b0705 ybfL -0.6 -0.4 putative receptor protein (VIMSS) compare
b3630 rfaP -0.6 -4.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1576 ydfD -0.6 -0.3 Qin prophage; predicted protein (NCBI) compare
b1235 rssB -0.6 -3.4 response regulator of RpoS (NCBI) compare
b1499 ydeO -0.6 -0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b3621 rfaC -0.6 -2.8 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b4702 mgtL -0.6 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b0685 ybfE -0.6 -2.6 orf, hypothetical protein (VIMSS) compare
b3075 ebgR -0.6 -3.1 DNA-binding transcriptional repressor (NCBI) compare
b0438 clpX -0.6 -3.8 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3636 rpmG -0.6 -1.5 50S ribosomal protein L33 (NCBI) compare
b0623 cspE -0.6 -1.8 cold shock protein E (NCBI) compare
b1855 lpxM -0.5 -2.8 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3180 yhbY -0.5 -3.1 predicted RNA-binding protein (NCBI) compare
b3469 zntA -0.5 -4.4 zinc, cobalt and lead efflux system (NCBI) compare
b2024 hisA -0.5 -1.8 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b1368 ynaA -0.5 -1.9 Rac prophage; predicted tail protein (RefSeq) compare
b3124 garK -0.5 -4.2 glycerate kinase I (RefSeq) compare
b0948 ycbY -0.5 -3.4 predicted methyltransferase (NCBI) compare
b2262 menB -0.5 -1.9 naphthoate synthase (NCBI) compare
b1804 rnd -0.5 -3.1 ribonuclease D (NCBI) compare
b1874 cutC -0.5 -1.4 copper homeostasis protein (NCBI) compare
b4201 priB -0.5 -1.4 primosomal replication protein N (NCBI) compare
b1088 yceD -0.5 -2.7 hypothetical protein (NCBI) compare
b1582 ynfA -0.5 -1.2 hypothetical protein (NCBI) compare
b0990 cspG -0.5 -1.0 DNA-binding transcriptional regulator (NCBI) compare
b0881 clpS -0.5 -1.1 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b4178 yjeB -0.5 -3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2352 yfdI -0.5 -0.7 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b4163 glyV -0.5 -1.2 tRNA-Gly (NCBI) compare
b1130 phoP -0.5 -0.9 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b0051 ksgA -0.5 -2.6 dimethyladenosine transferase (NCBI) compare
b3884 yihW -0.5 -3.5 putative DEOR-type transcriptional regulator (VIMSS) compare
b2512 yfgL -0.5 -3.1 protein assembly complex, lipoprotein component (NCBI) compare
b4153 frdB -0.5 -4.0 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b2104 thiM -0.5 -1.9 hydroxyethylthiazole kinase (NCBI) compare
b4179 vacB -0.5 -3.3 putative enzyme (VIMSS) compare
b3387 dam -0.5 -4.0 DNA adenine methylase (NCBI) compare
b0767 ybhE -0.5 -2.9 6-phosphogluconolactonase (NCBI) compare
b4383 deoB -0.5 -3.5 phosphopentomutase (NCBI) compare
b1690 ydiM -0.5 -1.7 predicted transporter (NCBI) compare
b4414 tff -0.5 -1.3 unknown RNA (NCBI) compare
b1630 rsxD -0.5 -2.9 electron transport complex protein RnfD (NCBI) compare
b1648 b1648 -0.5 -1.5 orf, hypothetical protein (VIMSS) compare
b3838 tatB -0.5 -3.2 sec-independent translocase (NCBI) compare
b2081 yegQ -0.5 -3.7 predicted peptidase (NCBI) compare
b1754 b1754 -0.5 -1.4 orf, hypothetical protein (VIMSS) compare
b1877 yecT -0.5 -1.7 orf, hypothetical protein (VIMSS) compare
b2642 yfjW -0.5 -0.9 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0912 ihfB -0.5 -1.2 integration host factor subunit beta (NCBI) compare
b0226 dinJ -0.5 -2.2 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3210 arcB -0.5 -2.7 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b2343 b2343 -0.5 -1.6 orf, hypothetical protein (VIMSS) compare
b1438 ydcQ -0.5 -1.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1253 yciA -0.5 -2.9 predicted hydrolase (NCBI) compare
b4443 gcvB -0.4 -0.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b2741 rpoS -0.4 -2.7 RNA polymerase sigma factor (NCBI) compare
b4436 ryeC -0.4 -0.9 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b2259 pmrD -0.4 -1.0 polymyxin resistance protein B (VIMSS) compare
b1540 ydfH -0.4 -1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2319 usg -0.4 -2.6 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms