Experiment set42IT054 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time48hr

200 most detrimental genes:

  gene name fitness t score description  
b1361 ydaW +1.1 1.0 orf, hypothetical protein (VIMSS) compare
b1185 dsbB +1.0 3.5 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA +0.9 8.5 periplasmic protein disulfide isomerase I (NCBI) compare
b4538 yoeF +0.8 2.0 hypothetical protein (RefSeq) compare
b1348 lar +0.7 2.1 Rac prophage; restriction alleviation protein (NCBI) compare
b2859 ygeO +0.7 1.9 hypothetical protein (RefSeq) compare
b0802 ybiJ +0.7 2.2 hypothetical protein (NCBI) compare
b1765 ydjA +0.7 1.1 predicted oxidoreductase (NCBI) compare
b1573 ydfC +0.6 1.2 Qin prophage; predicted protein (RefSeq) compare
b0630 lipB +0.6 1.1 protein of lipoate biosynthesis (VIMSS) compare
b0542 renD +0.6 2.3 orf, hypothetical protein (VIMSS) compare
b2156 lysP +0.6 5.3 lysine transporter (NCBI) compare
b2934 cmtB +0.6 0.5 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1476 fdnI +0.6 1.0 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1733 chbG +0.5 2.2 hypothetical protein (NCBI) compare
b1332 ynaJ +0.5 0.4 predicted inner membrane protein (NCBI) compare
b1357 ydaS +0.5 2.1 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0798 ybiA +0.5 1.9 hypothetical protein (NCBI) compare
b1686 ydiI +0.5 1.0 hypothetical protein (NCBI) compare
b3961 oxyR +0.4 3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b1195 ymgE +0.4 1.8 predicted inner membrane protein (NCBI) compare
b1558 cspF +0.4 1.9 Qin prophage; cold shock protein (NCBI) compare
b1753 ynjA +0.4 1.7 hypothetical protein (NCBI) compare
b4481 rffT +0.4 2.7 4-alpha-L-fucosyltransferase (NCBI) compare
b4596 yciZ +0.4 1.1 hypothetical protein (RefSeq) compare
b0183 rnhB +0.4 2.3 ribonuclease HII (NCBI) compare
b3786 rffE +0.4 3.5 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3120 yhaB +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1709 btuD +0.4 0.8 vitamin B12-transporter ATPase (NCBI) compare
b1143 ymfI +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1205 ychH +0.4 0.8 predicted inner membrane protein (NCBI) compare
b2399 yfeD +0.4 2.0 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4363 yjjB +0.4 2.4 conserved inner membrane protein (RefSeq) compare
b2915 yqfE +0.4 1.9 hypothetical protein (RefSeq) compare
b4698 mgrR +0.4 2.2 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b3280 yrdB +0.4 2.1 hypothetical protein (NCBI) compare
b0456 ybaA +0.4 1.8 hypothetical protein (NCBI) compare
b3791 rffA +0.4 2.9 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1070 flgN +0.4 1.0 export chaperone for FlgK and FlgL (NCBI) compare
b1134 ymfB +0.4 1.6 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b1724 ydiZ +0.4 1.5 hypothetical protein (NCBI) compare
b3794 rffM +0.4 2.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3787 rffD +0.4 3.0 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b4573 insZ +0.3 2.0 no description compare
b4653 yicT +0.3 0.9 no description compare
b1831 proQ +0.3 1.5 putative solute/DNA competence effector (NCBI) compare
b1530 marR +0.3 1.9 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b4640 yoeG +0.3 1.3 no description compare
b3790 rffC +0.3 2.3 TDP-fucosamine acetyltransferase (RefSeq) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b0946 ycbW +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b0212 gloB +0.3 1.8 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b1502 ydeQ +0.3 1.2 predicted fimbrial-like adhesin protein (NCBI) compare
b1448 yncA +0.3 0.9 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0521 ybcF +0.3 2.1 predicted carbamate kinase (NCBI) compare
b4699 fnrS +0.3 0.5 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b4406 yaeP +0.3 1.1 hypothetical protein (NCBI) compare
b2096 gatY +0.3 2.2 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2689 yqaA +0.3 2.0 conserved inner membrane protein (NCBI) compare
b2208 napF +0.3 1.8 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b1138 ymfE +0.3 0.9 e14 prophage; predicted inner membrane protein (NCBI) compare
b1434 ydcN +0.3 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0730 mngR +0.3 0.6 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3658 selC +0.3 1.3 tRNA-Sec (NCBI) compare
b2044 wcaL +0.3 1.3 predicted glycosyl transferase (NCBI) compare
b0944 ycbF +0.3 1.8 putative chaperone (VIMSS) compare
b4660 yhiL +0.3 0.2 no description compare
b1566 flxA +0.3 1.5 Qin prophage; predicted protein (NCBI) compare
b0393 rdgC +0.3 2.3 recombination associated protein (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b2587 kgtP +0.3 2.1 alpha-ketoglutarate transporter (NCBI) compare
b1252 tonB +0.3 1.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b0192 nlpE +0.3 2.2 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b1930 yedF +0.3 1.9 hypothetical protein (NCBI) compare
b4515 ybgT +0.3 1.0 hypothetical protein (NCBI) compare
b1656 sodB +0.3 1.2 superoxide dismutase, Fe (NCBI) compare
b3100 yqjK +0.3 2.0 hypothetical protein (NCBI) compare
b1849 purT +0.3 2.1 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1742 b1742 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2432 yfeY +0.3 1.3 hypothetical protein (NCBI) compare
b3206 npr +0.3 2.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b2051 wcaH +0.3 1.1 GDP-mannose mannosyl hydrolase (VIMSS) compare
b4042 dgkA +0.3 0.2 diacylglycerol kinase (NCBI) compare
b1408 b1408 +0.3 1.8 probable enzyme (VIMSS) compare
b4642 yoeD +0.3 1.0 no description compare
b4336 yjiN +0.3 2.2 conserved inner membrane protein (NCBI) compare
b2356 yfdM +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b4345 mcrC +0.3 0.5 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b2362 yfdS +0.3 1.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1339 abgR +0.3 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b0852 rimK +0.3 1.6 ribosomal protein S6 modification protein (NCBI) compare
b4687 shoB +0.3 1.6 toxic membrane protein (RefSeq) compare
b1038 csgF +0.3 1.4 predicted transport protein (NCBI) compare
b2264 menD +0.3 2.4 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1045 ymdB +0.3 1.7 hypothetical protein (NCBI) compare
b2433 b2433 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b1305 pspB +0.3 1.1 phage shock protein B (NCBI) compare
b1790 yeaM +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b4367 fhuF +0.3 2.2 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b1698 ydiR +0.3 1.5 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b1153 ymfQ +0.3 1.5 e14 prophage; conserved protein (NCBI) compare
b1603 pntA +0.3 1.7 NAD(P) transhydrogenase subunit alpha (NCBI) compare
b3784 rfe +0.3 2.4 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1520 b1520 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1858 znuC +0.3 2.0 high-affinity zinc transporter ATPase (NCBI) compare
b4360 yjjA +0.3 1.4 putative glycoprotein/receptor (VIMSS) compare
b1919 yedO +0.3 0.9 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1633 nth +0.3 1.8 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b0617 citD +0.3 0.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b3156 yhbS +0.3 2.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0641 rlpB +0.3 1.1 minor lipoprotein (NCBI) compare
b1768 ydjB +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1172 ymgG +0.3 1.8 conserved protein, UPF0757 family (RefSeq) compare
b3152 yraR +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0961 yccF +0.3 1.6 conserved inner membrane protein (NCBI) compare
b1961 dcm +0.3 0.6 DNA cytosine methylase (NCBI) compare
b1875 yecM +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b3623 rfaK +0.3 0.9 lipopolysaccharide core biosynthesis (NCBI) compare
b2565 recO +0.3 1.2 DNA repair protein RecO (NCBI) compare
b1847 yebF +0.3 0.7 predicted protein (RefSeq) compare
b0187 yaeR +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b0381 ddlA +0.3 1.9 D-alanylalanine synthetase (NCBI) compare
b2250 b2250 +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b1113 ycfS +0.2 1.9 hypothetical protein (NCBI) compare
b4144 yjeI +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b1977 asnT +0.2 1.1 tRNA-Asn (NCBI) compare
b2209 eco +0.2 0.9 ecotin precursor (NCBI) compare
b1480 sra +0.2 0.5 30S ribosomal subunit protein S22 (NCBI) compare
b1515 lsrD +0.2 1.6 AI2 transporter (NCBI) compare
b4045 yjbJ +0.2 1.0 predicted stress response protein (NCBI) compare
b2165 yeiN +0.2 1.6 hypothetical protein (NCBI) compare
b1063 yceB +0.2 1.1 predicted lipoprotein (NCBI) compare
b0682 ybfN +0.2 1.5 predicted lipoprotein (NCBI) compare
b4513 kdpF +0.2 0.8 potassium ion accessory transporter subunit (NCBI) compare
b2900 yqfB +0.2 1.5 hypothetical protein (NCBI) compare
b4376 osmY +0.2 1.5 periplasmic protein (NCBI) compare
b3099 yqjE +0.2 1.3 conserved inner membrane protein (NCBI) compare
b4157 yjeN +0.2 0.7 hypothetical protein (NCBI) compare
b3484 yhhI +0.2 1.0 predicted transposase (NCBI) compare
b2743 pcm +0.2 1.6 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b0843 ybjH +0.2 0.8 hypothetical protein (NCBI) compare
b1950 fliR +0.2 1.1 flagellar biosynthesis protein R (NCBI) compare
b1882 cheY +0.2 0.2 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1310 ycjN +0.2 1.6 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b0296 ykgM +0.2 1.4 50S ribosomal protein L31 (NCBI) compare
b1233 ychJ +0.2 1.3 hypothetical protein (NCBI) compare
b1355 b1355 +0.2 0.9 orf, hypothetical protein (VIMSS) compare
b3291 mscL +0.2 1.8 large-conductance mechanosensitive channel (NCBI) compare
b2195 ccmG +0.2 0.7 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b3198 kdsC +0.2 1.4 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2534 yfhR +0.2 1.9 predicted peptidase (RefSeq) compare
b1034 ycdX +0.2 1.4 hypothetical protein (NCBI) compare
b4015 aceA +0.2 1.4 isocitrate lyase (NCBI) compare
b3962 udhA +0.2 1.8 putative oxidoreductase (VIMSS) compare
b1789 yeaL +0.2 1.1 conserved inner membrane protein (NCBI) compare
b1144 ymfJ +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b2645 yfjZ +0.2 1.3 CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b1660 ydhC +0.2 1.3 predicted transporter (NCBI) compare
b1238 tdk +0.2 1.4 thymidine kinase (NCBI) compare
b1800 yeaU +0.2 1.7 predicted dehydrogenase (NCBI) compare
b3698 yidB +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b0844 b0844 +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b4387 ytjB +0.2 1.3 hypothetical protein (NCBI) compare
b0933 ssuB +0.2 1.2 alkanesulfonate transporter subunit (NCBI) compare
b1524 yneH +0.2 0.4 predicted glutaminase (NCBI) compare
b2996 hybA +0.2 1.9 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) compare
b2532 yfhQ +0.2 1.5 predicted methyltransferase (NCBI) compare
b4522 yciX_1 +0.2 0.8 hypothetical protein (NCBI) compare
b4571 wbbL +0.2 1.0 no description compare
b2010 dacD +0.2 1.5 penicillin binding protein 6b (VIMSS) compare
b1035 ycdY +0.2 1.5 hypothetical protein (NCBI) compare
b2782 chpA +0.2 1.4 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b1792 yeaO +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b0714 nei +0.2 1.6 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b1764 selD +0.2 1.1 selenophosphate synthetase (NCBI) compare
b0596 entA +0.2 1.3 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI) compare
b4686 yshB +0.2 1.0 expressed protein (RefSeq) compare
b4561 insM +0.2 1.1 transposase (RefSeq) compare
b1258 yciF +0.2 0.4 hypothetical protein (NCBI) compare
b4395 ytjC +0.2 1.1 phosphoglycerate mutase (NCBI) compare
b2488 hyfH +0.2 1.1 hydrogenase 4, Fe-S subunit (NCBI) compare
b1611 fumC +0.2 1.6 fumarate hydratase (NCBI) compare
b0731 mngA +0.2 1.5 fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI) compare
b3775 ppiC +0.2 0.9 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b3037 ygiB +0.2 1.2 conserved outer membrane protein (RefSeq) compare
b3163 nlpI +0.2 1.2 hypothetical protein (NCBI) compare
b2617 smpA +0.2 1.0 small membrane protein A (VIMSS) compare
b4358 yjjN +0.2 1.7 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq) compare
b1335 ogt +0.2 1.0 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b3844 fre +0.2 1.4 NAD(P)H-flavin reductase (NCBI) compare
b1323 tyrR +0.2 1.6 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b1427 rimL +0.2 0.8 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1287 yciW +0.2 1.2 putative oxidoreductase (VIMSS) compare
b1562 hokD +0.2 0.9 Qin prophage; small toxic polypeptide (NCBI) compare
b1963 b1963 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b2221 atoD +0.2 0.9 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b2769 ygcQ +0.2 1.4 putative flavoprotein (VIMSS) compare
b2103 thiD +0.2 1.5 phosphomethylpyrimidine kinase (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms