Experiment set42IT052 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time24hr

200 most detrimental genes:

  gene name fitness t score description  
b0630 lipB +1.4 2.7 protein of lipoate biosynthesis (VIMSS) compare
b0730 mngR +1.4 3.2 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1185 dsbB +1.3 6.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA +1.1 9.6 periplasmic protein disulfide isomerase I (NCBI) compare
b1831 proQ +0.8 2.5 putative solute/DNA competence effector (NCBI) compare
b1476 fdnI +0.8 1.4 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1205 ychH +0.8 1.7 predicted inner membrane protein (NCBI) compare
b1954 dsrA +0.7 3.4 regulatory antisense RNA (NCBI) compare
b1724 ydiZ +0.7 1.4 hypothetical protein (NCBI) compare
b0843 ybjH +0.7 2.0 hypothetical protein (NCBI) compare
b3512 GadE +0.7 4.0 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b4596 yciZ +0.7 1.9 hypothetical protein (RefSeq) compare
b4699 fnrS +0.7 1.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1524 yneH +0.6 1.2 predicted glutaminase (NCBI) compare
b4538 yoeF +0.6 2.0 hypothetical protein (RefSeq) compare
b1195 ymgE +0.6 2.3 predicted inner membrane protein (NCBI) compare
b3181 greA +0.6 4.0 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2156 lysP +0.6 5.0 lysine transporter (NCBI) compare
b0798 ybiA +0.5 1.8 hypothetical protein (NCBI) compare
b1144 ymfJ +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b1179 ycgL +0.5 2.3 conserved protein (NCBI) compare
b3027 ygiZ +0.5 0.3 conserved inner membrane protein (NCBI) compare
b1453 ansP +0.5 2.9 L-asparagine permease (VIMSS) compare
b3009 yghB +0.5 3.1 conserved inner membrane protein (NCBI) compare
b1448 yncA +0.5 1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2071 yegJ +0.5 1.9 hypothetical protein (NCBI) compare
b4646 yrdE +0.4 2.2 no description compare
b4431 rprA +0.4 1.7 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b4640 yoeG +0.4 1.7 no description compare
b1434 ydcN +0.4 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1348 lar +0.4 1.6 Rac prophage; restriction alleviation protein (NCBI) compare
b1742 b1742 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b1442 ydcU +0.4 2.3 predicted spermidine/putrescine transporter subunit (NCBI) compare
b0191 yaeJ +0.4 3.2 hypothetical protein (NCBI) compare
b2217 rcsB +0.4 3.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1739 osmE +0.4 1.9 DNA-binding transcriptional activator (NCBI) compare
b1824 yobF +0.4 1.5 hypothetical protein (NCBI) compare
b4157 yjeN +0.4 1.2 hypothetical protein (NCBI) compare
b0407 yajC +0.4 1.1 preprotein translocase subunit YajC (NCBI) compare
b3961 oxyR +0.4 3.6 DNA-binding transcriptional dual regulator (NCBI) compare
b3250 mreC +0.4 1.1 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b4613 dinQ +0.4 1.5 Damage inducible, function unknown (RefSeq) compare
b3236 mdh +0.4 3.4 malate dehydrogenase (NCBI) compare
b1792 yeaO +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b2741 rpoS +0.4 3.0 RNA polymerase sigma factor (NCBI) compare
b1427 rimL +0.4 2.0 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b2187 yejL +0.4 1.6 hypothetical protein (NCBI) compare
b2587 kgtP +0.4 2.4 alpha-ketoglutarate transporter (NCBI) compare
b0542 renD +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b1038 csgF +0.4 1.7 predicted transport protein (NCBI) compare
b1461 ydcE +0.4 2.0 4-oxalocrotonate tautomerase (NCBI) compare
b2208 napF +0.4 1.8 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b3037 ygiB +0.4 2.2 conserved outer membrane protein (RefSeq) compare
b1415 aldA +0.4 2.4 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b4480 hdfR +0.4 2.6 transcriptional regulator HdfR (NCBI) compare
b1357 ydaS +0.4 1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1382 ynbE +0.4 1.1 predicted lipoprotein (NCBI) compare
b1446 ydcY +0.4 1.7 hypothetical protein (NCBI) compare
b1823 cspC +0.4 1.4 stress protein, member of the CspA-family (NCBI) compare
b3153 yhbO +0.4 3.0 orf, hypothetical protein (VIMSS) compare
b4503 yafF +0.4 0.6 no description compare
b3261 fis +0.4 0.7 DNA-binding protein Fis (NCBI) compare
b2202 napC +0.4 2.1 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1610 tus +0.4 2.3 DNA replication terminus site-binding protein (NCBI) compare
b2616 recN +0.4 2.8 recombination and repair protein (NCBI) compare
b4042 dgkA +0.4 0.2 diacylglycerol kinase (NCBI) compare
b2398 yfeC +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1143 ymfI +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b4585 sroB +0.4 0.9 Novel sRNA, function unknown (RefSeq) compare
b1387 maoC +0.4 2.4 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b4492 ydbA +0.4 2.9 no description compare
b0183 rnhB +0.4 2.5 ribonuclease HII (NCBI) compare
b0521 ybcF +0.3 2.5 predicted carbamate kinase (NCBI) compare
b1463 nhoA +0.3 2.5 N-hydroxyarylamine O-acetyltransferase (NCBI) compare
b1414 ydcF +0.3 2.4 hypothetical protein (NCBI) compare
b1350 recE +0.3 2.4 Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI) compare
b2163 yeiL +0.3 2.2 putative transcriptional regulator (VIMSS) compare
b1152 ymfP +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b0880 cspD +0.3 1.4 cold shock protein homolog (NCBI) compare
b1930 yedF +0.3 2.5 hypothetical protein (NCBI) compare
b4218 ytfL +0.3 2.5 predicted inner membrane protein (NCBI) compare
b1487 ddpA +0.3 2.0 D-ala-D-a la transporter subunit (NCBI) compare
b0464 acrR +0.3 3.0 DNA-binding transcriptional repressor (NCBI) compare
b2264 menD +0.3 2.7 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1613 manA +0.3 1.1 mannose-6-phosphate isomerase (VIMSS) compare
b1882 cheY +0.3 0.4 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1063 yceB +0.3 1.8 predicted lipoprotein (NCBI) compare
b4394 yjjX +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b1344 ydaO +0.3 1.2 predicted C32 tRNA thiolase (NCBI) compare
b1408 b1408 +0.3 1.9 probable enzyme (VIMSS) compare
b1418 cybB +0.3 1.3 cytochrome b(561) (VIMSS) compare
b4693 aaaE +0.3 2.2 no description compare
b4551 yheV +0.3 1.4 hypothetical protein (NCBI) compare
b1764 selD +0.3 2.4 selenophosphate synthetase (NCBI) compare
b4660 yhiL +0.3 0.2 no description compare
b0846 ybjK +0.3 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b4642 yoeD +0.3 1.4 no description compare
b3021 ygiT +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1411 ynbD +0.3 2.1 predicted phosphatase, inner membrane protein (NCBI) compare
b4686 yshB +0.3 1.5 expressed protein (RefSeq) compare
b3627 rfaI +0.3 2.7 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b2435 amiA +0.3 1.8 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b4515 ybgT +0.3 1.0 hypothetical protein (NCBI) compare
b1419 ydcA +0.3 2.1 hypothetical protein (NCBI) compare
b3858 yihD +0.3 2.0 hypothetical protein (NCBI) compare
b1936 intG +0.3 0.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0929 ompF +0.3 2.0 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b1421 trg +0.3 2.2 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1233 ychJ +0.3 1.6 hypothetical protein (NCBI) compare
b1753 ynjA +0.3 1.4 hypothetical protein (NCBI) compare
b0623 cspE +0.3 1.1 cold shock protein E (NCBI) compare
b2051 wcaH +0.3 0.9 GDP-mannose mannosyl hydrolase (VIMSS) compare
b1633 nth +0.3 1.8 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b1530 marR +0.3 1.4 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1469 narU +0.3 1.9 nitrate/nitrite transporter (NCBI) compare
b3504 yhiS +0.3 0.2 hypothetical protein (RefSeq) compare
b1744 astE +0.3 2.2 succinylglutamate desuccinylase (NCBI) compare
b2911 ssrS +0.3 1.0 6S regulatory RNA (NCBI) compare
b1686 ydiI +0.3 0.6 hypothetical protein (NCBI) compare
b3100 yqjK +0.3 1.6 hypothetical protein (NCBI) compare
b2532 yfhQ +0.3 2.0 predicted methyltransferase (NCBI) compare
b2572 rseA +0.3 1.9 anti-sigma factor (NCBI) compare
b0613 citG +0.3 2.1 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b3790 rffC +0.3 1.9 TDP-fucosamine acetyltransferase (RefSeq) compare
b0933 ssuB +0.3 1.6 alkanesulfonate transporter subunit (NCBI) compare
b2195 ccmG +0.3 1.3 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1527 yneK +0.3 1.1 hypothetical protein (NCBI) compare
b4529 ydbJ +0.3 0.6 hypothetical protein (NCBI) compare
b2356 yfdM +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1070 flgN +0.3 0.8 export chaperone for FlgK and FlgL (NCBI) compare
b3623 rfaK +0.3 1.0 lipopolysaccharide core biosynthesis (NCBI) compare
b3870 glnA +0.3 1.4 glutamine synthetase (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b3120 yhaB +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b1342 ydaN +0.3 1.9 zinc transporter (NCBI) compare
b4450 arcZ +0.3 1.8 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b2672 ygaM +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b2433 b2433 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b1961 dcm +0.3 0.6 DNA cytosine methylase (NCBI) compare
b2261 menC +0.3 1.7 O-succinylbenzoate synthase (NCBI) compare
b0726 sucA +0.3 1.2 alpha-ketoglutarate decarboxylase (NCBI) compare
b2010 dacD +0.3 2.0 penicillin binding protein 6b (VIMSS) compare
b2618 b2618 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b2586 yfiM +0.3 0.9 predicted protein (RefSeq) compare
b2419 yfeK +0.3 1.1 hypothetical protein (NCBI) compare
b1976 b1976 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1467 narY +0.3 2.0 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b2209 eco +0.3 0.8 ecotin precursor (NCBI) compare
b2096 gatY +0.3 1.8 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1238 tdk +0.3 1.7 thymidine kinase (NCBI) compare
b2248 yfaX +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2708 gutQ +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1384 feaR +0.3 1.8 DNA-binding transcriptional dual regulator (NCBI) compare
b0803 ybiI +0.3 1.5 hypothetical protein (NCBI) compare
b0393 rdgC +0.3 2.1 recombination associated protein (NCBI) compare
b0209 yafD +0.3 2.1 hypothetical protein (NCBI) compare
b1795 yeaQ +0.3 1.7 conserved inner membrane protein (NCBI) compare
b3248 yhdE +0.3 2.1 Maf-like protein (NCBI) compare
b0192 nlpE +0.3 1.9 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b1429 tehA +0.3 0.4 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b4609 ryfD +0.3 1.7 Novel sRNA, function unknown (RefSeq) compare
b4363 yjjB +0.3 1.3 conserved inner membrane protein (RefSeq) compare
b0447 ybaO +0.3 2.1 putative LRP-like transcriptional regulator (VIMSS) compare
b4369 leuP +0.3 1.4 tRNA-Leu (NCBI) compare
b1625 ydgT +0.3 1.7 predicted regulator (NCBI) compare
b1790 yeaM +0.3 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1558 cspF +0.3 1.1 Qin prophage; cold shock protein (NCBI) compare
b2481 hyfA +0.3 1.7 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1172 ymgG +0.3 1.7 conserved protein, UPF0757 family (RefSeq) compare
b1383 ydbL +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b1074 flgC +0.3 1.1 flagellar basal-body rod protein C (NCBI) compare
b1768 ydjB +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1462 b1462 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b3889 yiiE +0.3 1.1 predicted transcriptional regulator (RefSeq) compare
b0178 hlpA +0.2 1.3 periplasmic chaperone (NCBI) compare
b2682 ygaZ +0.2 1.5 predicted transporter (NCBI) compare
b0296 ykgM +0.2 1.6 50S ribosomal protein L31 (NCBI) compare
b4675 yoaJ +0.2 1.0 expressed protein (RefSeq) compare
b2362 yfdS +0.2 1.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1368 ynaA +0.2 1.2 Rac prophage; predicted tail protein (RefSeq) compare
b1481 b1481 +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b3507 dctR +0.2 2.3 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b4410 ecnA +0.2 0.7 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1424 mdoD +0.2 1.6 glucan biosynthesis protein, periplasmic (RefSeq) compare
b0388 aroL +0.2 1.8 shikimate kinase II (NCBI) compare
b0018 mokC +0.2 0.7 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4573 insZ +0.2 1.6 no description compare
b2736 ygbJ +0.2 1.4 predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b1485 ddpC +0.2 1.6 D-ala-D-ala transporter subunit (NCBI) compare
b2058 wcaB +0.2 0.6 predicted acyl transferase (NCBI) compare
b1490 yddV +0.2 1.4 predicted diguanylate cyclase (RefSeq) compare
b3006 exbB +0.2 1.8 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3280 yrdB +0.2 1.6 hypothetical protein (NCBI) compare
b1072 flgA +0.2 1.0 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b1948 fliP +0.2 1.6 flagellar biosynthesis protein P (NCBI) compare
b0736 ybgC +0.2 1.0 predicted acyl-CoA thioesterase (NCBI) compare
b1858 znuC +0.2 1.6 high-affinity zinc transporter ATPase (NCBI) compare
b1892 flhD +0.2 1.6 DNA-binding transcriptional dual regulator with FlhC (RefSeq) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms