Experiment set42IT051 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time9hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -7.3 -15.6 alanine racemase (NCBI) compare
b4237 nrdG -5.4 -7.4 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.3 -11.8 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.8 -5.0 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -3.7 -13.0 glucose-6-phosphate isomerase (NCBI) compare
b1334 fnr -2.9 -3.7 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1850 eda -2.7 -3.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0386 proC -2.4 -7.1 pyrroline-5-carboxylate reductase (NCBI) compare
b0142 folK -2.0 -1.3 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2620 smpB -1.8 -1.9 SsrA-binding protein (NCBI) compare
b0949 uup -1.7 -12.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0243 proA -1.6 -10.2 gamma-glutamyl phosphate reductase (NCBI) compare
b0902 pflA -1.6 -9.3 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b2912 ygfA -1.5 -3.9 putative ligase (VIMSS) compare
b2846 yqeH -1.4 -1.1 orf, hypothetical protein (VIMSS) compare
b3916 pfkA -1.4 -4.0 6-phosphofructokinase (NCBI) compare
b1863 ruvC -1.4 -2.2 Holliday junction resolvase (NCBI) compare
b4402 yjjY -1.4 -1.5 hypothetical protein (NCBI) compare
b0242 proB -1.4 -6.3 gamma-glutamyl kinase (NCBI) compare
b1860 ruvB -1.4 -1.5 Holliday junction DNA helicase B (NCBI) compare
b1124 potC -1.3 -8.6 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2329 aroC -1.3 -6.8 chorismate synthase (NCBI) compare
b0688 pgm -1.2 -4.3 phosphoglucomutase (NCBI) compare
b4593 ymgI -1.2 -1.6 hypothetical protein (RefSeq) compare
b1236 galU -1.2 -5.4 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0200 gmhB -1.2 -1.9 hypothetical protein (NCBI) compare
b3781 trxA -1.2 -4.3 thioredoxin 1 (VIMSS) compare
b0303 ykgI -1.2 -1.0 orf, hypothetical protein (VIMSS) compare
b3390 aroK -1.1 -2.2 shikimate kinase I (NCBI) compare
b1126 potA -1.1 -6.6 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b2297 pta -1.1 -0.7 phosphate acetyltransferase (NCBI) compare
b1292 sapC -1.1 -2.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1291 sapD -1.1 -1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1551 b1551 -1.1 -0.7 orf, hypothetical protein (VIMSS) compare
b0739 tolA -1.1 -2.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2830 nudH -1.1 -1.6 dinucleoside polyphosphate hydrolase (NCBI) compare
b1123 potD -1.1 -5.9 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b1125 potB -1.1 -6.0 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0738 tolR -1.0 -1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0728 sucC -1.0 -5.4 succinyl-CoA synthetase subunit beta (NCBI) compare
b3405 ompR -1.0 -4.6 osmolarity response regulator (NCBI) compare
b3868 glnG -1.0 -6.1 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0903 pflB -1.0 -7.9 pyruvate formate lyase I (NCBI) compare
b0729 sucD -1.0 -3.3 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2820 recB -1.0 -0.9 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3229 sspA -1.0 -2.6 stringent starvation protein A (NCBI) compare
b3162 deaD -1.0 -4.7 inducible ATP-independent RNA helicase (VIMSS) compare
b0908 aroA -1.0 -6.3 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b1048 mdoG -1.0 -5.7 glucan biosynthesis protein, periplasmic (NCBI) compare
b3052 rfaE -0.9 -4.7 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2659 b2659 -0.9 -2.4 orf, hypothetical protein (VIMSS) compare
b3620 rfaF -0.9 -5.4 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1593 b1593 -0.9 -4.1 orf, hypothetical protein (VIMSS) compare
b1984 asnW -0.9 -1.8 tRNA-Asn (NCBI) compare
b0912 ihfB -0.9 -1.9 integration host factor subunit beta (NCBI) compare
b2528 iscA -0.9 -1.5 iron-sulfur cluster assembly protein (NCBI) compare
b1347 ydaC -0.9 -0.5 Rac prophage; predicted protein (NCBI) compare
b0737 tolQ -0.9 -2.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0990 cspG -0.9 -1.8 DNA-binding transcriptional regulator (NCBI) compare
b2450 yffS -0.9 -2.6 CPZ-55 prophage; predicted protein (RefSeq) compare
b2330 yfcB -0.9 -1.3 putative adenine-specific methylase (VIMSS) compare
b1231 tyrT -0.9 -1.6 tRNA-Tyr (NCBI) compare
b1286 rnb -0.9 -2.6 exoribonuclease II (NCBI) compare
b1049 mdoH -0.9 -6.9 glucosyltransferase MdoH (NCBI) compare
b0740 tolB -0.9 -2.5 translocation protein TolB precursor (NCBI) compare
b3842 rfaH -0.8 -5.0 transcriptional activator RfaH (NCBI) compare
b2894 xerD -0.8 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b0053 surA -0.8 -5.3 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3281 aroE -0.8 -7.7 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b4375 prfC -0.8 -2.6 peptide chain release factor 3 (NCBI) compare
b1861 ruvA -0.8 -1.1 Holliday junction DNA helicase motor protein (NCBI) compare
b0232 yafN -0.8 -1.1 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3164 pnp -0.8 -2.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3389 aroB -0.8 -6.6 3-dehydroquinate synthase (NCBI) compare
b4535 yniD -0.8 -1.7 predicted protein (RefSeq) compare
b3493 pitA -0.8 -5.5 phosphate transporter, low-affinity (NCBI) compare
b3930 menA -0.8 -6.8 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1370 insH-5 -0.8 -2.9 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b3631 rfaG -0.8 -7.0 glucosyltransferase I (NCBI) compare
b3500 gor -0.8 -2.0 glutathione reductase (NCBI) compare
b0603 ybdO -0.8 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b0890 ftsK -0.8 -2.9 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b4685 yrbN -0.8 -2.3 expressed protein (RefSeq) compare
b1192 ldcA -0.7 -1.7 L,D-carboxypeptidase A (NCBI) compare
b1536 ydeI -0.7 -1.0 hypothetical protein (NCBI) compare
b2501 ppk -0.7 -5.5 polyphosphate kinase (NCBI) compare
b2110 yehC -0.7 -1.6 predicted periplasmic pilin chaperone (NCBI) compare
b1259 yciG -0.7 -2.4 orf, hypothetical protein (VIMSS) compare
b1865 nudB -0.7 -1.3 dATP pyrophosphohydrolase (NCBI) compare
b1855 lpxM -0.7 -3.4 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3204 ptsN -0.7 -4.1 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3035 tolC -0.7 -3.1 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2569 lepA -0.7 -3.4 GTP-binding protein LepA (NCBI) compare
b3630 rfaP -0.7 -5.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0437 clpP -0.7 -3.3 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1497 b1497 -0.7 -0.4 putative enzyme (VIMSS) compare
b1001 yccE -0.7 -0.3 hypothetical protein (NCBI) compare
b4552 yrhC -0.7 -1.2 no description compare
b1182 hlyE -0.6 -0.5 hemolysin E (VIMSS) compare
b4179 vacB -0.6 -4.4 putative enzyme (VIMSS) compare
b4503 yafF -0.6 -0.9 no description compare
b0500 ybbD -0.6 -0.3 hypothetical protein (RefSeq) compare
b1165 ymgA -0.6 -0.3 hypothetical protein (NCBI) compare
b1167 ymgC -0.6 -0.3 hypothetical protein (NCBI) compare
b2567 rnc -0.6 -0.3 ribonuclease III (NCBI) compare
b2055 wcaE -0.6 -0.3 predicted glycosyl transferase (NCBI) compare
b2652 ileY -0.6 -0.8 tRNA-Ile (NCBI) compare
b3619 rfaD -0.6 -2.5 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2343 b2343 -0.6 -2.3 orf, hypothetical protein (VIMSS) compare
b2576 srmB -0.6 -2.0 ATP-dependent RNA helicase (NCBI) compare
b4647 mokA -0.6 -1.0 no description compare
b2814 metZ -0.6 -1.6 tRNA-Met (NCBI) compare
b0051 ksgA -0.6 -3.0 dimethyladenosine transferase (NCBI) compare
b0237 pepD -0.6 -4.6 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b2318 truA -0.6 -1.9 tRNA pseudouridine synthase A (NCBI) compare
b0438 clpX -0.6 -3.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0705 ybfL -0.6 -0.4 putative receptor protein (VIMSS) compare
b0118 acnB -0.6 -0.7 aconitate hydratase (NCBI) compare
b0149 mrcB -0.6 -5.0 penicillin-binding protein 1b (NCBI) compare
b1147 ymfL -0.6 -2.8 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b4201 priB -0.6 -1.4 primosomal replication protein N (NCBI) compare
b4131 cadA -0.6 -5.3 lysine decarboxylase 1 (NCBI) compare
b1235 rssB -0.6 -3.7 response regulator of RpoS (NCBI) compare
b1108 ycfP -0.6 -1.4 orf, hypothetical protein (VIMSS) compare
b0049 apaH -0.6 -1.5 diadenosinetetraphosphatase (NCBI) compare
b1842 holE -0.6 -1.1 DNA polymerase III, theta subunit (NCBI) compare
b0014 dnaK -0.6 -2.0 molecular chaperone DnaK (NCBI) compare
b1499 ydeO -0.6 -0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1576 ydfD -0.6 -0.3 Qin prophage; predicted protein (NCBI) compare
b0741 pal -0.6 -2.0 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2081 yegQ -0.6 -4.8 predicted peptidase (NCBI) compare
b0957 ompA -0.6 -2.4 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b1648 b1648 -0.6 -1.7 orf, hypothetical protein (VIMSS) compare
b1363 trkG -0.5 -0.4 Rac prophage; potassium transporter subunit (NCBI) compare
b1804 rnd -0.5 -2.6 ribonuclease D (NCBI) compare
b4462 ygaQ -0.5 -1.6 hypothetical protein (RefSeq) compare
b2017 yefM -0.5 -0.7 orf, hypothetical protein (VIMSS) compare
b2259 pmrD -0.5 -1.2 polymyxin resistance protein B (VIMSS) compare
b3806 cyaA -0.5 -3.3 adenylate cyclase (NCBI) compare
b3119 tdcR -0.5 -2.7 threonine dehydratase operon activator protein (VIMSS) compare
b1351 racC -0.5 -0.4 Rac prophage; predicted protein (NCBI) compare
b0167 glnD -0.5 -2.1 PII uridylyl-transferase (NCBI) compare
b3075 ebgR -0.5 -3.0 DNA-binding transcriptional repressor (NCBI) compare
b0881 clpS -0.5 -1.5 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b0222 lpcA -0.5 -1.7 phosphoheptose isomerase (NCBI) compare
b3977 tyrU -0.5 -0.7 tRNA-Tyr (NCBI) compare
b1693 aroD -0.5 -3.3 3-dehydroquinate dehydratase (NCBI) compare
b4066 yjcF -0.5 -0.5 hypothetical protein (NCBI) compare
b1677 lpp -0.5 -1.3 murein lipoprotein (NCBI) compare
b4443 gcvB -0.5 -0.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b1712 ihfA -0.5 -0.6 integration host factor subunit alpha (NCBI) compare
b4178 yjeB -0.5 -3.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1918 yecS -0.5 -2.3 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4413 sokC -0.5 -1.5 regulatory antisense RNA (NCBI) compare
b0658 ybeX -0.5 -0.5 predicteed ion transport (NCBI) compare
b3728 pstS -0.5 -2.3 phosphate transporter subunit (NCBI) compare
b4202 rpsR -0.5 -1.1 30S ribosomal protein S18 (NCBI) compare
b1309 ycjM -0.5 -2.8 putative polysaccharide hydrolase (VIMSS) compare
b3158 yhbU -0.5 -3.8 predicted peptidase (collagenase-like) (NCBI) compare
b4132 cadB -0.5 -4.2 predicted lysine/cadaverine transporter (NCBI) compare
b2105 yohL -0.5 -2.1 hypothetical protein (NCBI) compare
b1579 intQ -0.5 -2.1 Qin prophage; predicted defective integrase (RefSeq) compare
b3159 yhbV -0.5 -3.9 orf, hypothetical protein (VIMSS) compare
b3741 gidA -0.5 -1.7 glucose-inhibited division protein A (NCBI) compare
b2847 yqeI -0.5 -0.3 predicted transcriptional regulator (NCBI) compare
b0234 yafP -0.4 -1.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0080 fruR -0.4 -2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b1290 sapF -0.4 -1.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3621 rfaC -0.4 -2.3 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2853 ygeI -0.4 -0.2 hypothetical protein (NCBI) compare
b2683 ygaH -0.4 -2.7 predicted inner membrane protein (NCBI) compare
b0145 dksA -0.4 -1.8 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3706 trmE -0.4 -1.9 tRNA modification GTPase (NCBI) compare
b2948 yqgE -0.4 -1.3 orf, hypothetical protein (VIMSS) compare
b3387 dam -0.4 -3.4 DNA adenine methylase (NCBI) compare
b1241 adhE -0.4 -3.3 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b4163 glyV -0.4 -1.0 tRNA-Gly (NCBI) compare
b0549 ybcO -0.4 -0.7 DLP12 prophage; predicted protein (NCBI) compare
b4151 frdD -0.4 -1.8 fumarate reductase subunit D (NCBI) compare
b1058 yceO -0.4 -0.2 hypothetical protein (NCBI) compare
b2562 yfhL -0.4 -3.5 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b1258 yciF -0.4 -0.6 hypothetical protein (NCBI) compare
b3839 tatC -0.4 -2.4 TatABCE protein translocation system subunit (NCBI) compare
b0564 appY -0.4 -0.9 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1853 yebK -0.4 -2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2024 hisA -0.4 -1.8 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2479 gcvR -0.4 -2.3 transcriptional regulation of gcv operon (VIMSS) compare
b2082 ogrK -0.4 -1.1 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b2111 yehD -0.4 -2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b0948 ycbY -0.4 -2.8 predicted methyltransferase (NCBI) compare
b2393 nupC -0.4 -2.9 nucleoside (except guanosine) transporter (NCBI) compare
b4133 cadC -0.4 -3.8 DNA-binding transcriptional activator (NCBI) compare
b0975 hyaD -0.4 -2.6 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b0125 hpt -0.4 -1.8 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b1130 phoP -0.4 -0.8 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b3545 proK -0.4 -1.0 tRNA-Pro (NCBI) compare
b4545 ypdJ -0.4 -1.4 no description compare
b1627 rsxA -0.4 -1.8 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1012 b1012 -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b2498 upp -0.4 -1.2 uracil phosphoribosyltransferase (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms