Experiment set42IT051 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time9hr

200 most detrimental genes:

  gene name fitness t score description  
b3860 dsbA +1.0 8.8 periplasmic protein disulfide isomerase I (NCBI) compare
b1361 ydaW +1.0 0.8 orf, hypothetical protein (VIMSS) compare
b1185 dsbB +1.0 5.2 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1831 proQ +0.6 2.6 putative solute/DNA competence effector (NCBI) compare
b0798 ybiA +0.6 2.0 hypothetical protein (NCBI) compare
b1195 ymgE +0.6 2.0 predicted inner membrane protein (NCBI) compare
b4481 rffT +0.6 4.4 4-alpha-L-fucosyltransferase (NCBI) compare
b1305 pspB +0.6 2.3 phage shock protein B (NCBI) compare
b2156 lysP +0.6 5.3 lysine transporter (NCBI) compare
b3790 rffC +0.6 4.3 TDP-fucosamine acetyltransferase (RefSeq) compare
b1348 lar +0.6 1.9 Rac prophage; restriction alleviation protein (NCBI) compare
b1480 sra +0.6 1.9 30S ribosomal subunit protein S22 (NCBI) compare
b3021 ygiT +0.6 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b4505 insX +0.6 1.3 predicted IS protein (RefSeq) compare
b2934 cmtB +0.5 0.5 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1882 cheY +0.5 0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b0542 renD +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b4596 yciZ +0.5 1.2 hypothetical protein (RefSeq) compare
b4551 yheV +0.5 2.2 hypothetical protein (NCBI) compare
b1742 b1742 +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b3786 rffE +0.5 4.3 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b4345 mcrC +0.5 1.0 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1765 ydjA +0.5 0.8 predicted oxidoreductase (NCBI) compare
b3623 rfaK +0.5 1.7 lipopolysaccharide core biosynthesis (NCBI) compare
b0183 rnhB +0.5 2.8 ribonuclease HII (NCBI) compare
b4128 yjdK +0.5 0.4 hypothetical protein (NCBI) compare
b1823 cspC +0.5 1.5 stress protein, member of the CspA-family (NCBI) compare
b2208 napF +0.4 2.2 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b0998 torD +0.4 1.0 chaperone protein TorD (NCBI) compare
b1476 fdnI +0.4 0.7 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1954 dsrA +0.4 2.0 regulatory antisense RNA (NCBI) compare
b3787 rffD +0.4 3.7 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1724 ydiZ +0.4 1.4 hypothetical protein (NCBI) compare
b1558 cspF +0.4 1.8 Qin prophage; cold shock protein (NCBI) compare
b2187 yejL +0.4 1.9 hypothetical protein (NCBI) compare
b3624 rfaZ +0.4 1.7 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2481 hyfA +0.4 2.9 hydrogenase 4 Fe-S subunit (VIMSS) compare
b3784 rfe +0.4 3.6 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1566 flxA +0.4 2.1 Qin prophage; predicted protein (NCBI) compare
b3961 oxyR +0.4 3.4 DNA-binding transcriptional dual regulator (NCBI) compare
b1533 ydeD +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1346 ydaQ +0.4 0.7 orf, hypothetical protein (VIMSS) compare
b2433 b2433 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b0802 ybiJ +0.4 1.7 hypothetical protein (NCBI) compare
b0218 yafU +0.4 1.5 predicted inner membrane protein (RefSeq) compare
b3396 mrcA +0.4 3.6 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b2504 yfgG +0.4 0.8 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b1183 umuD +0.4 1.3 DNA polymerase V, subunit D (NCBI) compare
b1144 ymfJ +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1273 yciN +0.4 1.2 hypothetical protein (NCBI) compare
b3794 rffM +0.4 2.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1758 b1758 +0.4 0.9 putative cytochrome oxidase (VIMSS) compare
b0730 mngR +0.4 0.7 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4642 yoeD +0.3 1.1 no description compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.3 0.8 hypothetical protein (NCBI) compare
b0946 ycbW +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2809 b2809 +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b2419 yfeK +0.3 1.5 hypothetical protein (NCBI) compare
b3791 rffA +0.3 2.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2743 pcm +0.3 1.8 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b1252 tonB +0.3 1.5 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1919 yedO +0.3 1.2 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b4277 yjgZ +0.3 2.6 KpLE2 phage-like element; predicted protein (NCBI) compare
b3163 nlpI +0.3 2.0 hypothetical protein (NCBI) compare
b2193 narP +0.3 2.3 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b1485 ddpC +0.3 1.8 D-ala-D-ala transporter subunit (NCBI) compare
b0114 aceE +0.3 0.8 pyruvate dehydrogenase subunit E1 (NCBI) compare
b3147 yraM +0.3 3.0 hypothetical protein (NCBI) compare
b1686 ydiI +0.3 0.7 hypothetical protein (NCBI) compare
b4538 yoeF +0.3 0.9 hypothetical protein (RefSeq) compare
b2510 b2510 +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b4157 yjeN +0.3 0.9 hypothetical protein (NCBI) compare
b1357 ydaS +0.3 1.3 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4042 dgkA +0.3 0.2 diacylglycerol kinase (NCBI) compare
b3238 yhcN +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1332 ynaJ +0.3 0.3 predicted inner membrane protein (NCBI) compare
b2532 yfhQ +0.3 2.0 predicted methyltransferase (NCBI) compare
b2792 yqcC +0.3 1.0 hypothetical protein (NCBI) compare
b0429 cyoD +0.3 1.5 cytochrome o ubiquinol oxidase subunit IV (NCBI) compare
b3027 ygiZ +0.3 0.2 conserved inner membrane protein (NCBI) compare
b2399 yfeD +0.3 1.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1930 yedF +0.3 1.9 hypothetical protein (NCBI) compare
b4441 glmY +0.3 1.0 sRNA activator of glmS mRNA (RefSeq) compare
b4520 ymgF +0.3 0.9 hypothetical protein (NCBI) compare
b1007 b1007 +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b0101 yacG +0.3 1.6 zinc-binding protein (NCBI) compare
b1085 b1085 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b4519 icdC +0.3 1.7 no description compare
b1233 ychJ +0.3 2.1 hypothetical protein (NCBI) compare
b1955 yedP +0.3 1.5 hypothetical protein (NCBI) compare
b4603 rseX +0.3 0.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1205 ychH +0.3 1.0 predicted inner membrane protein (NCBI) compare
b0210 yafE +0.3 1.2 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2409 yfeR +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0852 rimK +0.3 2.1 ribosomal protein S6 modification protein (NCBI) compare
b0580 ybdJ +0.3 1.0 predicted inner membrane protein (NCBI) compare
b4480 hdfR +0.3 1.8 transcriptional regulator HdfR (NCBI) compare
b1227 narI +0.3 1.5 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b3037 ygiB +0.3 1.8 conserved outer membrane protein (RefSeq) compare
b1976 b1976 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b2579 yfiD +0.3 0.9 pyruvate formate lyase subunit (NCBI) compare
b1557 cspB +0.3 0.6 Qin prophage; cold shock protein (NCBI) compare
b2261 menC +0.3 2.0 O-succinylbenzoate synthase (NCBI) compare
b1418 cybB +0.3 1.4 cytochrome b(561) (VIMSS) compare
b2023 hisH +0.3 1.8 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b0641 rlpB +0.3 1.0 minor lipoprotein (NCBI) compare
b4573 insZ +0.3 1.6 no description compare
b0819 ybiS +0.3 2.1 hypothetical protein (NCBI) compare
b4692 oweE +0.3 1.3 no description compare
b3512 GadE +0.3 1.7 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1847 yebF +0.3 0.6 predicted protein (RefSeq) compare
b4699 fnrS +0.3 0.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1342 ydaN +0.3 1.7 zinc transporter (NCBI) compare
b2488 hyfH +0.3 1.0 hydrogenase 4, Fe-S subunit (NCBI) compare
b1249 cls +0.3 1.8 cardiolipin synthetase (NCBI) compare
b0209 yafD +0.3 1.8 hypothetical protein (NCBI) compare
b4701 sokX +0.3 0.4 sok-related sRNA, function unknown (RefSeq) compare
b1830 prc +0.3 1.8 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1672 ydhW +0.3 1.3 hypothetical protein (NCBI) compare
b2500 purN +0.3 1.7 phosphoribosylglycinamide formyltransferase (NCBI) compare
b0393 rdgC +0.3 2.1 recombination associated protein (NCBI) compare
b2998 yghW +0.3 1.6 hypothetical protein (NCBI) compare
b0388 aroL +0.3 1.7 shikimate kinase II (NCBI) compare
b1562 hokD +0.3 1.2 Qin prophage; small toxic polypeptide (NCBI) compare
b0803 ybiI +0.3 1.4 hypothetical protein (NCBI) compare
b0247 ykfG +0.3 0.7 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b3206 npr +0.3 1.5 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4376 osmY +0.3 1.4 periplasmic protein (NCBI) compare
b0714 nei +0.3 1.5 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b1625 ydgT +0.3 1.7 predicted regulator (NCBI) compare
b1448 yncA +0.2 1.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3152 yraR +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b4418 sraB +0.2 0.4 unknown RNA (NCBI) compare
b3723 bglG +0.2 1.7 transcriptional antiterminator of the bgl operon (NCBI) compare
b2096 gatY +0.2 1.5 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1074 flgC +0.2 1.1 flagellar basal-body rod protein C (NCBI) compare
b1469 narU +0.2 1.3 nitrate/nitrite transporter (NCBI) compare
b2962 yggX +0.2 1.5 hypothetical protein (NCBI) compare
b1961 dcm +0.2 0.5 DNA cytosine methylase (NCBI) compare
b1421 trg +0.2 1.5 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1600 mdtJ +0.2 0.7 multidrug efflux system transporter (NCBI) compare
b1153 ymfQ +0.2 0.9 e14 prophage; conserved protein (NCBI) compare
b1113 ycfS +0.2 1.7 hypothetical protein (NCBI) compare
b3775 ppiC +0.2 1.3 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b1660 ydhC +0.2 1.3 predicted transporter (NCBI) compare
b0942 ycbU +0.2 1.6 predicted fimbrial-like adhesin protein (NCBI) compare
b2199 ccmC +0.2 1.5 heme exporter subunit (NCBI) compare
b0521 ybcF +0.2 1.4 predicted carbamate kinase (NCBI) compare
b1586 b1586 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b0456 ybaA +0.2 1.0 hypothetical protein (NCBI) compare
b2486 hyfF +0.2 1.7 NADH dehydrogenase subunit N (NCBI) compare
b3476 nikA +0.2 2.0 nickel transporter subunit (NCBI) compare
b1251 yciI +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b2587 kgtP +0.2 1.7 alpha-ketoglutarate transporter (NCBI) compare
b0944 ycbF +0.2 1.6 putative chaperone (VIMSS) compare
b3622 rfaL +0.2 1.3 O-antigen ligase (NCBI) compare
b4367 fhuF +0.2 1.8 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b2741 rpoS +0.2 1.6 RNA polymerase sigma factor (NCBI) compare
b4592 yccB +0.2 0.8 hypothetical protein (RefSeq) compare
b2388 glk +0.2 1.6 glucokinase (NCBI) compare
b2368 emrK +0.2 1.8 EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component (NCBI) compare
b1238 tdk +0.2 1.5 thymidine kinase (NCBI) compare
b1663 mdtK +0.2 1.6 multidrug efflux protein NorM (NCBI) compare
b4360 yjjA +0.2 1.3 putative glycoprotein/receptor (VIMSS) compare
b2302 yfcG +0.2 1.7 predicted glutathione S-transferase (NCBI) compare
b1325 ycjG +0.2 1.0 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b0245 ykfI +0.2 1.8 CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b2535 csiE +0.2 2.1 orf, hypothetical protein (VIMSS) compare
b1299 puuR +0.2 1.4 DNA-binding transcriptional repressor (NCBI) compare
b1399 paaX +0.2 1.4 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b4686 yshB +0.2 0.9 expressed protein (RefSeq) compare
b1248 b1248 +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b4698 mgrR +0.2 1.2 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b2804 fucU +0.2 1.2 L-fucose mutarotase (NCBI) compare
b3626 rfaJ +0.2 1.5 UDP-D-glucose:(galactosyl)lipopolysaccharide glucosyltransferase (NCBI) compare
b0547 ybcN +0.2 1.5 DLP12 prophage; predicted protein (NCBI) compare
b1768 ydjB +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b1776 ydjL +0.2 1.4 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b4511 ybdZ +0.2 1.5 hypothetical protein (NCBI) compare
b2996 hybA +0.2 1.9 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) compare
b4532 yncN +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b1511 ydeV +0.2 1.2 putative kinase (VIMSS) compare
b1848 yebG +0.2 0.6 conserved protein regulated by LexA (NCBI) compare
b2014 yeeF +0.2 1.4 putative amino acid/amine transport protein (VIMSS) compare
b4000 hupA +0.2 1.5 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b4656 yjhY +0.2 1.3 no description compare
b2154 yeiG +0.2 1.2 predicted esterase (NCBI) compare
b2264 menD +0.2 1.7 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1698 ydiR +0.2 1.4 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b2284 nuoF +0.2 1.4 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b3698 yidB +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b1339 abgR +0.2 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1733 chbG +0.2 0.9 hypothetical protein (NCBI) compare
b0909 ycaL +0.2 1.2 putative heat shock protein (VIMSS) compare
b1575 dicB +0.2 0.6 Qin prophage; cell division inhibition protein (NCBI) compare
b1349 recT +0.2 1.4 Rac prophage; recombination and repair protein (NCBI) compare
b2209 eco +0.2 0.5 ecotin precursor (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms