Experiment set42IT019 for Escherichia coli BW25113

Compare to:

LB regrowth after 48hrs

200 most important genes:

  gene name fitness t score description  
b4053 alr -8.4 -5.8 alanine racemase (NCBI) compare
b0949 uup -6.0 -16.8 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4237 nrdG -5.9 -4.1 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.5 -3.8 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -5.2 -4.5 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -4.8 -13.5 glucose-6-phosphate isomerase (NCBI) compare
b1850 eda -4.4 -5.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0386 proC -4.0 -4.7 pyrroline-5-carboxylate reductase (NCBI) compare
b1334 fnr -3.9 -2.7 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b3183 obgE -3.3 -2.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0145 dksA -3.2 -4.4 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3390 aroK -3.2 -4.2 shikimate kinase I (NCBI) compare
b1123 potD -3.2 -11.5 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b3052 rfaE -3.1 -8.1 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1124 potC -3.0 -10.5 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b4218 ytfL -3.0 -17.4 predicted inner membrane protein (NCBI) compare
b2330 yfcB -3.0 -2.0 putative adenine-specific methylase (VIMSS) compare
b0688 pgm -2.8 -5.8 phosphoglucomutase (NCBI) compare
b0222 lpcA -2.8 -4.8 phosphoheptose isomerase (NCBI) compare
b3781 trxA -2.8 -7.1 thioredoxin 1 (VIMSS) compare
b4402 yjjY -2.8 -1.6 hypothetical protein (NCBI) compare
b1125 potB -2.8 -10.6 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1126 potA -2.7 -15.2 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b0902 pflA -2.7 -12.4 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b0243 proA -2.6 -16.4 gamma-glutamyl phosphate reductase (NCBI) compare
b0737 tolQ -2.6 -3.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2912 ygfA -2.5 -4.0 putative ligase (VIMSS) compare
b3621 rfaC -2.5 -6.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2576 srmB -2.4 -6.3 ATP-dependent RNA helicase (NCBI) compare
b3405 ompR -2.4 -7.1 osmolarity response regulator (NCBI) compare
b1236 galU -2.4 -7.2 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0741 pal -2.3 -5.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0242 proB -2.2 -8.6 gamma-glutamyl kinase (NCBI) compare
b3619 rfaD -2.2 -4.6 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0053 surA -2.2 -10.7 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1677 lpp -2.1 -4.7 murein lipoprotein (NCBI) compare
b2620 smpB -2.1 -2.3 SsrA-binding protein (NCBI) compare
b0739 tolA -2.1 -3.5 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3930 menA -2.1 -17.1 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b3842 rfaH -2.1 -8.0 transcriptional activator RfaH (NCBI) compare
b3839 tatC -2.1 -8.4 TatABCE protein translocation system subunit (NCBI) compare
b4202 rpsR -2.1 -3.0 30S ribosomal protein S18 (NCBI) compare
b3500 gor -2.0 -3.6 glutathione reductase (NCBI) compare
b3620 rfaF -1.9 -8.1 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0014 dnaK -1.9 -4.3 molecular chaperone DnaK (NCBI) compare
b3229 sspA -1.9 -3.8 stringent starvation protein A (NCBI) compare
b3162 deaD -1.9 -6.9 inducible ATP-independent RNA helicase (VIMSS) compare
b0740 tolB -1.9 -4.0 translocation protein TolB precursor (NCBI) compare
b0142 folK -1.8 -1.3 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2017 yefM -1.8 -1.6 orf, hypothetical protein (VIMSS) compare
b3868 glnG -1.8 -8.2 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b2329 aroC -1.8 -9.9 chorismate synthase (NCBI) compare
b1860 ruvB -1.8 -1.9 Holliday junction DNA helicase B (NCBI) compare
b4375 prfC -1.8 -2.4 peptide chain release factor 3 (NCBI) compare
b3035 tolC -1.8 -5.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2830 nudH -1.7 -2.2 dinucleoside polyphosphate hydrolase (NCBI) compare
b0167 glnD -1.7 -5.6 PII uridylyl-transferase (NCBI) compare
b3977 tyrU -1.7 -1.6 tRNA-Tyr (NCBI) compare
b1804 rnd -1.7 -7.5 ribonuclease D (NCBI) compare
b0903 pflB -1.7 -12.4 pyruvate formate lyase I (NCBI) compare
b0200 gmhB -1.7 -1.9 hypothetical protein (NCBI) compare
b0237 pepD -1.6 -10.9 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b0082 mraW -1.6 -5.1 S-adenosyl-methyltransferase (NCBI) compare
b0908 aroA -1.6 -10.1 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3493 pitA -1.5 -9.5 phosphate transporter, low-affinity (NCBI) compare
b4685 yrbN -1.5 -5.1 expressed protein (RefSeq) compare
b3916 pfkA -1.5 -3.9 6-phosphofructokinase (NCBI) compare
b4414 tff -1.5 -3.8 unknown RNA (NCBI) compare
b3389 aroB -1.5 -11.4 3-dehydroquinate synthase (NCBI) compare
b1049 mdoH -1.5 -10.9 glucosyltransferase MdoH (NCBI) compare
b0912 ihfB -1.5 -3.1 integration host factor subunit beta (NCBI) compare
b2808 gcvA -1.4 -5.5 DNA-binding transcriptional dual regulator (NCBI) compare
b4593 ymgI -1.4 -1.8 hypothetical protein (RefSeq) compare
b3281 aroE -1.3 -11.6 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b2501 ppk -1.3 -9.7 polyphosphate kinase (NCBI) compare
b3164 pnp -1.3 -4.0 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1048 mdoG -1.3 -7.1 glucan biosynthesis protein, periplasmic (NCBI) compare
b0438 clpX -1.3 -7.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b4171 miaA -1.2 -4.1 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b3630 rfaP -1.2 -9.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2297 pta -1.2 -0.8 phosphate acetyltransferase (NCBI) compare
b2528 iscA -1.2 -2.1 iron-sulfur cluster assembly protein (NCBI) compare
b2597 yfiA -1.2 -3.0 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b2318 truA -1.2 -3.6 tRNA pseudouridine synthase A (NCBI) compare
b4131 cadA -1.2 -10.7 lysine decarboxylase 1 (NCBI) compare
b1551 b1551 -1.2 -0.7 orf, hypothetical protein (VIMSS) compare
b0439 lon -1.2 -4.4 DNA-binding ATP-dependent protease La (NCBI) compare
b3706 trmE -1.2 -3.9 tRNA modification GTPase (NCBI) compare
b3741 gidA -1.2 -2.8 glucose-inhibited division protein A (NCBI) compare
b4201 priB -1.2 -3.2 primosomal replication protein N (NCBI) compare
b0728 sucC -1.1 -6.5 succinyl-CoA synthetase subunit beta (NCBI) compare
b2894 xerD -1.1 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b2659 b2659 -1.1 -2.2 orf, hypothetical protein (VIMSS) compare
b3636 rpmG -1.1 -2.1 50S ribosomal protein L33 (NCBI) compare
b4177 purA -1.1 -2.1 adenylosuccinate synthetase (NCBI) compare
b3387 dam -1.1 -8.7 DNA adenine methylase (NCBI) compare
b3631 rfaG -1.1 -9.7 glucosyltransferase I (NCBI) compare
b1693 aroD -1.1 -5.6 3-dehydroquinate dehydratase (NCBI) compare
b1292 sapC -1.1 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0948 ycbY -1.1 -3.8 predicted methyltransferase (NCBI) compare
b3158 yhbU -1.1 -8.6 predicted peptidase (collagenase-like) (NCBI) compare
b0738 tolR -1.1 -1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4179 vacB -1.0 -6.5 putative enzyme (VIMSS) compare
b1187 fadR -1.0 -3.1 fatty acid metabolism regulator (NCBI) compare
b0232 yafN -1.0 -1.3 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3375 yhfR -1.0 -7.9 putative transcriptional regulator (VIMSS) compare
b0729 sucD -1.0 -4.7 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1855 lpxM -1.0 -4.4 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b2820 recB -1.0 -0.9 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b4132 cadB -1.0 -8.9 predicted lysine/cadaverine transporter (NCBI) compare
b2569 lepA -1.0 -3.7 GTP-binding protein LepA (NCBI) compare
b3159 yhbV -1.0 -8.1 orf, hypothetical protein (VIMSS) compare
b1658 purR -1.0 -5.0 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1332 ynaJ -1.0 -0.9 predicted inner membrane protein (NCBI) compare
b3180 yhbY -1.0 -3.7 predicted RNA-binding protein (NCBI) compare
b1192 ldcA -1.0 -2.4 L,D-carboxypeptidase A (NCBI) compare
b3247 cafA -1.0 -8.1 bundles of cytoplasmic filaments (VIMSS) compare
b3806 cyaA -1.0 -5.4 adenylate cyclase (NCBI) compare
b1347 ydaC -1.0 -0.5 Rac prophage; predicted protein (NCBI) compare
b2393 nupC -0.9 -6.6 nucleoside (except guanosine) transporter (NCBI) compare
b0890 ftsK -0.9 -3.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2512 yfgL -0.9 -4.8 protein assembly complex, lipoprotein component (NCBI) compare
b1088 yceD -0.9 -4.7 hypothetical protein (NCBI) compare
b1863 ruvC -0.9 -1.5 Holliday junction resolvase (NCBI) compare
b1712 ihfA -0.9 -0.9 integration host factor subunit alpha (NCBI) compare
b1182 hlyE -0.9 -0.5 hemolysin E (VIMSS) compare
b3884 yihW -0.9 -5.4 putative DEOR-type transcriptional regulator (VIMSS) compare
b1293 sapB -0.9 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0437 clpP -0.9 -4.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b4154 frdA -0.9 -7.5 fumarate reductase (NCBI) compare
b0049 apaH -0.9 -2.0 diadenosinetetraphosphatase (NCBI) compare
b0051 ksgA -0.8 -4.4 dimethyladenosine transferase (NCBI) compare
b1613 manA -0.8 -2.0 mannose-6-phosphate isomerase (VIMSS) compare
b2363 yfdT -0.8 -3.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4178 yjeB -0.8 -5.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2479 gcvR -0.8 -4.4 transcriptional regulation of gcv operon (VIMSS) compare
b4163 glyV -0.8 -2.2 tRNA-Gly (NCBI) compare
b3911 cpxA -0.8 -4.0 two-component sensor protein (NCBI) compare
b1235 rssB -0.8 -5.9 response regulator of RpoS (NCBI) compare
b2343 b2343 -0.8 -2.2 orf, hypothetical protein (VIMSS) compare
b3157 yhbT -0.8 -3.7 predicted lipid carrier protein (NCBI) compare
b2024 hisA -0.8 -2.4 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b1286 rnb -0.8 -1.8 exoribonuclease II (NCBI) compare
b4647 mokA -0.8 -1.1 no description compare
b3404 envZ -0.8 -4.9 osmolarity sensor protein (NCBI) compare
b0303 ykgI -0.8 -0.9 orf, hypothetical protein (VIMSS) compare
b4384 deoD -0.8 -6.5 purine nucleoside phosphorylase (NCBI) compare
b3792 wzxE -0.8 -4.5 O-antigen translocase (NCBI) compare
b1291 sapD -0.8 -1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0990 cspG -0.8 -1.1 DNA-binding transcriptional regulator (NCBI) compare
b0234 yafP -0.8 -1.8 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3871 bipA -0.8 -6.6 GTP-binding protein (NCBI) compare
b2531 iscR -0.8 -1.9 DNA-binding transcriptional repressor (NCBI) compare
b0080 fruR -0.8 -4.4 DNA-binding transcriptional dual regulator (NCBI) compare
b0149 mrcB -0.8 -6.5 penicillin-binding protein 1b (NCBI) compare
b3613 yibP -0.8 -1.0 putative membrane protein (VIMSS) compare
b3609 secB -0.8 -3.2 export protein SecB (NCBI) compare
b3253 yhdH -0.8 -5.8 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b2055 wcaE -0.7 -0.4 predicted glycosyl transferase (NCBI) compare
b2625 yfjI -0.7 -1.7 CP4-57 prophage; predicted protein (NCBI) compare
b3205 yhbJ -0.7 -3.2 hypothetical protein (NCBI) compare
b1001 yccE -0.7 -0.4 hypothetical protein (NCBI) compare
b3761 trpT -0.7 -1.8 tRNA-Trp (NCBI) compare
b1258 yciF -0.7 -0.9 hypothetical protein (NCBI) compare
b4153 frdB -0.7 -5.2 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b2553 glnB -0.7 -3.8 regulatory protein P-II for glutamine synthetase (NCBI) compare
b2498 upp -0.7 -2.1 uracil phosphoribosyltransferase (VIMSS) compare
b4191 ulaR -0.7 -3.5 DNA-binding transcriptional dual regulator (NCBI) compare
b3728 pstS -0.7 -3.1 phosphate transporter subunit (NCBI) compare
b0226 dinJ -0.7 -2.7 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1253 yciA -0.7 -3.4 predicted hydrolase (NCBI) compare
b3838 tatB -0.7 -3.3 sec-independent translocase (NCBI) compare
b0500 ybbD -0.7 -0.3 hypothetical protein (RefSeq) compare
b3210 arcB -0.7 -3.5 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b4493 gapC -0.7 -1.5 no description compare
b1594 dgsA -0.7 -3.8 DNA-binding transcriptional repressor (NCBI) compare
b4654 yjdQ -0.7 -4.3 no description compare
b1593 b1593 -0.7 -3.8 orf, hypothetical protein (VIMSS) compare
b1159 mcrA -0.7 -1.4 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2683 ygaH -0.7 -3.2 predicted inner membrane protein (NCBI) compare
b2652 ileY -0.7 -0.8 tRNA-Ile (NCBI) compare
b1104 ycfL -0.7 -1.3 hypothetical protein (NCBI) compare
b1167 ymgC -0.7 -0.3 hypothetical protein (NCBI) compare
b0436 tig -0.7 -4.7 trigger factor (NCBI) compare
b3545 proK -0.7 -1.5 tRNA-Pro (NCBI) compare
b2081 yegQ -0.7 -4.7 predicted peptidase (NCBI) compare
b1499 ydeO -0.7 -0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1576 ydfD -0.7 -0.3 Qin prophage; predicted protein (NCBI) compare
b1290 sapF -0.6 -1.6 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4497 yeeL -0.6 -1.1 no description compare
b2418 pdxK -0.6 -4.4 pyridoxine kinase (NCBI) compare
b0603 ybdO -0.6 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3408 feoA -0.6 -5.3 ferrous iron transport protein A (NCBI) compare
b4138 dcuA -0.6 -5.5 C4-dicarboxylate antiporter (NCBI) compare
b0953 rmf -0.6 -1.6 ribosome modulation factor (NCBI) compare
b3204 ptsN -0.6 -3.3 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2567 rnc -0.6 -0.3 ribonuclease III (NCBI) compare
b3612 gpmI -0.6 -5.9 phosphoglyceromutase (NCBI) compare
b4133 cadC -0.6 -5.8 DNA-binding transcriptional activator (NCBI) compare
b1346 ydaQ -0.6 -1.4 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 4 genes in this experiment