Experiment set42IT018 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time48hr

200 most detrimental genes:

  gene name fitness t score description  
b1185 dsbB +1.5 8.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1361 ydaW +1.4 1.3 orf, hypothetical protein (VIMSS) compare
b3860 dsbA +1.1 10.0 periplasmic protein disulfide isomerase I (NCBI) compare
b0630 lipB +1.0 1.9 protein of lipoate biosynthesis (VIMSS) compare
b1831 proQ +1.0 3.8 putative solute/DNA competence effector (NCBI) compare
b1882 cheY +0.9 1.1 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b2934 cmtB +0.8 0.7 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2859 ygeO +0.8 2.0 hypothetical protein (RefSeq) compare
b0798 ybiA +0.7 2.1 hypothetical protein (NCBI) compare
b1205 ychH +0.6 1.4 predicted inner membrane protein (NCBI) compare
b1195 ymgE +0.6 1.8 predicted inner membrane protein (NCBI) compare
b4699 fnrS +0.6 0.9 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1357 ydaS +0.5 2.1 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b2911 ssrS +0.5 1.9 6S regulatory RNA (NCBI) compare
b4701 sokX +0.5 0.7 sok-related sRNA, function unknown (RefSeq) compare
b3120 yhaB +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b1530 marR +0.5 2.2 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b4642 yoeD +0.5 1.9 no description compare
b2208 napF +0.5 2.5 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b4363 yjjB +0.5 3.3 conserved inner membrane protein (RefSeq) compare
b1478 adhP +0.5 2.3 alcohol dehydrogenase (VIMSS) compare
b1724 ydiZ +0.5 1.6 hypothetical protein (NCBI) compare
b0464 acrR +0.5 4.1 DNA-binding transcriptional repressor (NCBI) compare
b1742 b1742 +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b1434 ydcN +0.5 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1919 yedO +0.5 1.6 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1739 osmE +0.5 2.1 DNA-binding transcriptional activator (NCBI) compare
b4538 yoeF +0.5 1.1 hypothetical protein (RefSeq) compare
b4660 yhiL +0.4 0.3 no description compare
b2096 gatY +0.4 2.8 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2433 b2433 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1558 cspF +0.4 1.9 Qin prophage; cold shock protein (NCBI) compare
b0802 ybiJ +0.4 1.6 hypothetical protein (NCBI) compare
b3961 oxyR +0.4 3.8 DNA-binding transcriptional dual regulator (NCBI) compare
b3624 rfaZ +0.4 1.7 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1399 paaX +0.4 2.9 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b2217 rcsB +0.4 3.4 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1954 dsrA +0.4 2.0 regulatory antisense RNA (NCBI) compare
b2263 yfbB +0.4 1.4 predicted peptidase (NCBI) compare
b1348 lar +0.4 1.4 Rac prophage; restriction alleviation protein (NCBI) compare
b2156 lysP +0.4 3.7 lysine transporter (NCBI) compare
b1476 fdnI +0.4 0.7 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b4157 yjeN +0.4 1.0 hypothetical protein (NCBI) compare
b1847 yebF +0.4 0.8 predicted protein (RefSeq) compare
b1753 ynjA +0.4 1.3 hypothetical protein (NCBI) compare
b3512 GadE +0.4 2.6 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0726 sucA +0.4 2.0 alpha-ketoglutarate decarboxylase (NCBI) compare
b0929 ompF +0.4 2.4 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b0183 rnhB +0.4 2.5 ribonuclease HII (NCBI) compare
b1238 tdk +0.4 2.6 thymidine kinase (NCBI) compare
b1950 fliR +0.4 1.7 flagellar biosynthesis protein R (NCBI) compare
b4431 rprA +0.4 1.3 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1134 ymfB +0.4 1.5 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b0191 yaeJ +0.4 2.8 hypothetical protein (NCBI) compare
b3181 greA +0.4 2.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2248 yfaX +0.4 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b4693 aaaE +0.4 2.5 no description compare
b1625 ydgT +0.4 2.1 predicted regulator (NCBI) compare
b1414 ydcF +0.4 2.2 hypothetical protein (NCBI) compare
b1977 asnT +0.4 1.6 tRNA-Asn (NCBI) compare
b1756 ynjD +0.4 1.8 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0535 fimZ +0.4 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b4042 dgkA +0.4 0.2 diacylglycerol kinase (NCBI) compare
b1419 ydcA +0.4 2.4 hypothetical protein (NCBI) compare
b4551 yheV +0.3 1.5 hypothetical protein (NCBI) compare
b2481 hyfA +0.3 1.8 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1438 ydcQ +0.3 1.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0571 cusR +0.3 2.2 DNA-binding response regulator in two-component regulatory system with CusS (NCBI) compare
b4570 lomR +0.3 1.3 no description compare
b1058 yceO +0.3 0.2 hypothetical protein (NCBI) compare
b4451 ryhB +0.3 0.7 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b4218 ytfL +0.3 2.6 predicted inner membrane protein (NCBI) compare
b1463 nhoA +0.3 2.5 N-hydroxyarylamine O-acetyltransferase (NCBI) compare
b1408 b1408 +0.3 2.3 probable enzyme (VIMSS) compare
b1822 rrmA +0.3 1.3 23S rRNA m1G745 methyltransferase (NCBI) compare
b1113 ycfS +0.3 2.4 hypothetical protein (NCBI) compare
b1151 ymfO +0.3 2.0 e14 prophage; conserved protein (RefSeq) compare
b1418 cybB +0.3 1.1 cytochrome b(561) (VIMSS) compare
b1410 ynbC +0.3 2.6 predicted hydrolase (NCBI) compare
b2250 b2250 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1698 ydiR +0.3 1.0 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b0880 cspD +0.3 1.3 cold shock protein homolog (NCBI) compare
b1442 ydcU +0.3 1.7 predicted spermidine/putrescine transporter subunit (NCBI) compare
b4609 ryfD +0.3 1.4 Novel sRNA, function unknown (RefSeq) compare
b1153 ymfQ +0.3 1.6 e14 prophage; conserved protein (NCBI) compare
b3129 sohA +0.3 2.1 predicted regulator (NCBI) compare
b1610 tus +0.3 2.0 DNA replication terminus site-binding protein (NCBI) compare
b1415 aldA +0.3 2.4 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b0418 pgpA +0.3 2.1 phosphatidylglycerophosphatase A (NCBI) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b2506 yfgI +0.3 1.5 hypothetical protein (NCBI) compare
b3068 ygjF +0.3 1.8 G/U mismatch-specific DNA glycosylase (NCBI) compare
b2010 dacD +0.3 2.1 penicillin binding protein 6b (VIMSS) compare
b1930 yedF +0.3 2.5 hypothetical protein (NCBI) compare
b4387 ytjB +0.3 1.9 hypothetical protein (NCBI) compare
b3009 yghB +0.3 2.5 conserved inner membrane protein (NCBI) compare
b3844 fre +0.3 2.2 NAD(P)H-flavin reductase (NCBI) compare
b1963 b1963 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b0388 aroL +0.3 1.9 shikimate kinase II (NCBI) compare
b1144 ymfJ +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b0998 torD +0.3 0.7 chaperone protein TorD (NCBI) compare
b1573 ydfC +0.3 0.6 Qin prophage; predicted protein (RefSeq) compare
b2743 pcm +0.3 1.9 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b3630 rfaP +0.3 2.5 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0937 ssuE +0.3 1.3 NAD(P)H-dependent FMN reductase (NCBI) compare
b1161 ycgX +0.3 0.5 hypothetical protein (NCBI) compare
b2684 mprA +0.3 1.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1063 yceB +0.3 1.7 predicted lipoprotein (NCBI) compare
b2765 ygcM +0.3 1.2 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b0840 deoR +0.3 2.0 DNA-binding transcriptional repressor (NCBI) compare
b0447 ybaO +0.3 2.1 putative LRP-like transcriptional regulator (VIMSS) compare
b0942 ycbU +0.3 2.2 predicted fimbrial-like adhesin protein (NCBI) compare
b2095 gatZ +0.3 2.1 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b1138 ymfE +0.3 0.8 e14 prophage; predicted inner membrane protein (NCBI) compare
b0624 crcB +0.3 1.8 camphor resistance protein CrcB (NCBI) compare
b2953 yggU +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0209 yafD +0.3 2.2 hypothetical protein (NCBI) compare
b0730 mngR +0.3 0.6 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b1179 ycgL +0.3 0.9 conserved protein (NCBI) compare
b1553 ydfP +0.3 0.5 Qin prophage; conserved protein (NCBI) compare
b1448 yncA +0.3 1.0 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1684 sufA +0.3 1.5 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1955 yedP +0.3 1.2 hypothetical protein (NCBI) compare
b4492 ydbA +0.3 2.3 no description compare
b0754 aroG +0.3 2.0 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b1164 ycgZ +0.3 0.7 hypothetical protein (NCBI) compare
b0821 ybiU +0.3 1.9 hypothetical protein (NCBI) compare
b2488 hyfH +0.3 1.2 hydrogenase 4, Fe-S subunit (NCBI) compare
b1823 cspC +0.3 0.9 stress protein, member of the CspA-family (NCBI) compare
b1515 lsrD +0.3 1.9 AI2 transporter (NCBI) compare
b1395 paaH +0.3 1.9 3-hydroxybutyryl-CoA dehydrogenase (NCBI) compare
b2284 nuoF +0.3 1.6 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2741 rpoS +0.3 1.7 RNA polymerase sigma factor (NCBI) compare
b3037 ygiB +0.3 1.7 conserved outer membrane protein (RefSeq) compare
b1462 b1462 +0.3 0.6 orf, hypothetical protein (VIMSS) compare
b1768 ydjB +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b2261 menC +0.3 1.9 O-succinylbenzoate synthase (NCBI) compare
b0613 citG +0.3 1.6 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b1936 intG +0.3 0.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3021 ygiT +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b0114 aceE +0.3 0.6 pyruvate dehydrogenase subunit E1 (NCBI) compare
b1362 rzpR +0.3 1.1 putative Rac prophage endopeptidase (VIMSS) compare
b2264 menD +0.3 2.0 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b2435 amiA +0.3 1.9 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1427 rimL +0.3 1.1 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b0193 yaeF +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b1053 mdtG +0.3 1.5 predicted drug efflux system (NCBI) compare
b4603 rseX +0.3 0.3 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b0713 ybgL +0.3 1.9 hypothetical protein (NCBI) compare
b1345 intR +0.3 2.1 Rac prophage; integrase (NCBI) compare
b2708 gutQ +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b0803 ybiI +0.3 1.2 hypothetical protein (NCBI) compare
b4063 soxR +0.3 1.5 DNA-binding transcriptional dual regulator, Fe-S center for redox-sensing (NCBI) compare
b0393 rdgC +0.2 2.0 recombination associated protein (NCBI) compare
b2959 yggL +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b0848 ybjM +0.2 1.7 predicted inner membrane protein (NCBI) compare
b1387 maoC +0.2 1.8 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1443 ydcV +0.2 1.3 predicted spermidine/putrescine transporter subunit (NCBI) compare
b0853 ybjN +0.2 1.5 predicted oxidoreductase (NCBI) compare
b1575 dicB +0.2 0.7 Qin prophage; cell division inhibition protein (NCBI) compare
b4596 yciZ +0.2 0.5 hypothetical protein (RefSeq) compare
b1765 ydjA +0.2 0.4 predicted oxidoreductase (NCBI) compare
b2193 narP +0.2 1.7 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b2587 kgtP +0.2 1.5 alpha-ketoglutarate transporter (NCBI) compare
b3136 agaS +0.2 1.5 tagatose-6-phosphate ketose/aldose isomerase (NCBI) compare
b2510 b2510 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b2565 recO +0.2 1.3 DNA repair protein RecO (NCBI) compare
b1527 yneK +0.2 0.9 hypothetical protein (NCBI) compare
b4002 yjaI +0.2 1.8 orf, hypothetical protein (VIMSS) compare
b2876 b2876 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b1411 ynbD +0.2 1.3 predicted phosphatase, inner membrane protein (NCBI) compare
b1000 cbpA +0.2 1.7 curved DNA-binding protein, DnaJ homologue that functions as a co-chaperone of DnaK (NCBI) compare
b2500 purN +0.2 1.8 phosphoribosylglycinamide formyltransferase (NCBI) compare
b3100 yqjK +0.2 1.4 hypothetical protein (NCBI) compare
b3623 rfaK +0.2 0.8 lipopolysaccharide core biosynthesis (NCBI) compare
b1421 trg +0.2 1.2 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b4367 fhuF +0.2 1.8 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b3752 rbsK +0.2 2.1 ribokinase (NCBI) compare
b2200 ccmB +0.2 1.4 heme exporter subunit (NCBI) compare
b1841 yobA +0.2 0.7 hypothetical protein (NCBI) compare
b4550 yhdL +0.2 1.4 hypothetical protein (NCBI) compare
b3761 trpT +0.2 0.8 tRNA-Trp (NCBI) compare
b0618 citC +0.2 1.8 citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS) compare
b0228 yafM +0.2 1.4 hypothetical protein (NCBI) compare
b0481 ybaK +0.2 1.1 hypothetical protein (NCBI) compare
b4618 tisB +0.2 1.3 lexA-regulated toxic peptide (RefSeq) compare
b0323 yahI +0.2 1.4 predicted carbamate kinase-like protein (NCBI) compare
b2555 yfhG +0.2 1.5 hypothetical protein (NCBI) compare
b3153 yhbO +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b4543 ypaA +0.2 0.9 predicted protein (NCBI) compare
b2759 ygcK +0.2 1.6 hypothetical protein (NCBI) compare
b4411 ecnB +0.2 1.4 entericidin B membrane lipoprotein (NCBI) compare
b0456 ybaA +0.2 0.9 hypothetical protein (NCBI) compare
b3361 fic +0.2 1.3 stationary-phase protein, cell division (NCBI) compare
b0442 ybaV +0.2 1.7 hypothetical protein (NCBI) compare
b2094 gatA +0.2 1.7 galactitol-specific enzyme IIA component of PTS (NCBI) compare
b0933 ssuB +0.2 1.4 alkanesulfonate transporter subunit (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment

For stress Carbenicillin disodium salt in Escherichia coli BW25113

For stress Carbenicillin disodium salt across organisms