Experiment set42IT018 for Escherichia coli BW25113

LB + carbenicillin Time48hr

Group: stress
Media: LB + Carbenicillin disodium salt (0.0044 mg/mL), pH=7
Culturing: Keio_ML9a, 1L bottle, Anaerobic, at 37 (C), shaken=0 rpm
By: Charlotte Brannon/Becca Culver on 21-Oct-20
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 4 genes in this experiment

For stress Carbenicillin disodium salt in Escherichia coli BW25113

For stress Carbenicillin disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	1
Heat shock dnaK gene cluster extended	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Thiamine metabolism
• Folate biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UTP and CTP dephosphorylation I	7	5	2
pyrimidine deoxyribonucleotides de novo biosynthesis IV	7	6	1
pyrimidine deoxyribonucleotides biosynthesis from CTP	8	6	1
pyrimidine deoxyribonucleotides de novo biosynthesis I	9	9	1
pyrimidine deoxyribonucleotides de novo biosynthesis III	9	8	1
peptidoglycan recycling II	10	7	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	14	1
peptidoglycan recycling I	14	14	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	1