Experiment set42IT016 for Escherichia coli BW25113

Compare to:

LB Time24hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -6.6 -16.9 alanine racemase (NCBI) compare
b4237 nrdG -5.2 -7.1 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.0 -11.6 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.0 -5.0 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -3.5 -14.0 glucose-6-phosphate isomerase (NCBI) compare
b1850 eda -2.5 -3.2 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1860 ruvB -2.4 -1.6 Holliday junction DNA helicase B (NCBI) compare
b0949 uup -2.3 -12.1 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0386 proC -2.1 -6.1 pyrroline-5-carboxylate reductase (NCBI) compare
b1334 fnr -2.0 -3.1 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2620 smpB -2.0 -2.0 SsrA-binding protein (NCBI) compare
b4402 yjjY -1.9 -1.4 hypothetical protein (NCBI) compare
b4593 ymgI -1.8 -1.9 hypothetical protein (RefSeq) compare
b0902 pflA -1.7 -7.1 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b0243 proA -1.6 -10.8 gamma-glutamyl phosphate reductase (NCBI) compare
b2830 nudH -1.4 -1.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b1123 potD -1.4 -8.1 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b3781 trxA -1.4 -6.2 thioredoxin 1 (VIMSS) compare
b0912 ihfB -1.4 -2.6 integration host factor subunit beta (NCBI) compare
b3183 obgE -1.4 -1.4 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0142 folK -1.3 -1.0 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b4202 rpsR -1.3 -2.4 30S ribosomal protein S18 (NCBI) compare
b4375 prfC -1.2 -2.9 peptide chain release factor 3 (NCBI) compare
b0741 pal -1.2 -3.7 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0242 proB -1.2 -5.4 gamma-glutamyl kinase (NCBI) compare
b1124 potC -1.2 -7.6 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2329 aroC -1.2 -5.6 chorismate synthase (NCBI) compare
b0737 tolQ -1.2 -2.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0739 tolA -1.1 -2.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2318 truA -1.1 -2.7 tRNA pseudouridine synthase A (NCBI) compare
b3916 pfkA -1.1 -3.3 6-phosphofructokinase (NCBI) compare
b1677 lpp -1.1 -2.7 murein lipoprotein (NCBI) compare
b1551 b1551 -1.0 -0.6 orf, hypothetical protein (VIMSS) compare
b0908 aroA -1.0 -6.5 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3405 ompR -1.0 -3.3 osmolarity response regulator (NCBI) compare
b1048 mdoG -1.0 -5.5 glucan biosynthesis protein, periplasmic (NCBI) compare
b4552 yrhC -1.0 -1.4 no description compare
b3811 xerC -1.0 -1.3 site-specific tyrosine recombinase XerC (NCBI) compare
b1236 galU -1.0 -4.2 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0903 pflB -0.9 -7.3 pyruvate formate lyase I (NCBI) compare
b3281 aroE -0.9 -8.5 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b1126 potA -0.9 -4.8 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b1358 ydaT -0.9 -1.0 Rac prophage; predicted protein (NCBI) compare
b1049 mdoH -0.9 -6.5 glucosyltransferase MdoH (NCBI) compare
b1861 ruvA -0.9 -0.9 Holliday junction DNA helicase motor protein (NCBI) compare
b3706 trmE -0.9 -3.1 tRNA modification GTPase (NCBI) compare
b3390 aroK -0.9 -1.9 shikimate kinase I (NCBI) compare
b1505 ydeT -0.9 -1.3 hypothetical protein (RefSeq) compare
b1370 insH-5 -0.9 -3.5 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b2912 ygfA -0.8 -2.2 putative ligase (VIMSS) compare
b3389 aroB -0.8 -6.4 3-dehydroquinate synthase (NCBI) compare
b2569 lepA -0.8 -3.7 GTP-binding protein LepA (NCBI) compare
b1347 ydaC -0.8 -0.5 Rac prophage; predicted protein (NCBI) compare
b4451 ryhB -0.8 -1.7 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b3741 gidA -0.8 -2.8 glucose-inhibited division protein A (NCBI) compare
b1286 rnb -0.8 -2.2 exoribonuclease II (NCBI) compare
b3035 tolC -0.8 -3.2 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1863 ruvC -0.8 -1.4 Holliday junction resolvase (NCBI) compare
b0437 clpP -0.8 -4.0 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2894 xerD -0.8 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b2531 iscR -0.8 -1.6 DNA-binding transcriptional repressor (NCBI) compare
b1291 sapD -0.8 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0082 mraW -0.8 -3.2 S-adenosyl-methyltransferase (NCBI) compare
b0237 pepD -0.7 -5.1 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b1865 nudB -0.7 -1.5 dATP pyrophosphohydrolase (NCBI) compare
b3162 deaD -0.7 -3.6 inducible ATP-independent RNA helicase (VIMSS) compare
b4647 mokA -0.7 -1.1 no description compare
b0890 ftsK -0.7 -2.6 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2501 ppk -0.7 -5.1 polyphosphate kinase (NCBI) compare
b2330 yfcB -0.7 -1.0 putative adenine-specific methylase (VIMSS) compare
b3229 sspA -0.7 -1.9 stringent starvation protein A (NCBI) compare
b4163 glyV -0.7 -1.9 tRNA-Gly (NCBI) compare
b4201 priB -0.7 -2.2 primosomal replication protein N (NCBI) compare
b3619 rfaD -0.7 -2.7 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0603 ybdO -0.7 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3868 glnG -0.7 -3.6 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0738 tolR -0.7 -1.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3493 pitA -0.7 -4.6 phosphate transporter, low-affinity (NCBI) compare
b3341 rpsG -0.7 -1.5 30S ribosomal protein S7 (NCBI) compare
b0688 pgm -0.7 -2.4 phosphoglucomutase (NCBI) compare
b3636 rpmG -0.7 -2.0 50S ribosomal protein L33 (NCBI) compare
b1693 aroD -0.6 -3.6 3-dehydroquinate dehydratase (NCBI) compare
b1593 b1593 -0.6 -2.6 orf, hypothetical protein (VIMSS) compare
b1125 potB -0.6 -2.7 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b4577 sgrS -0.6 -1.6 small antisense RNA (NCBI) compare
b0658 ybeX -0.6 -0.5 predicteed ion transport (NCBI) compare
b4066 yjcF -0.6 -0.6 hypothetical protein (NCBI) compare
b1882 cheY -0.6 -0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3158 yhbU -0.6 -5.1 predicted peptidase (collagenase-like) (NCBI) compare
b0438 clpX -0.6 -3.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1497 b1497 -0.6 -0.4 putative enzyme (VIMSS) compare
b1001 yccE -0.6 -0.3 hypothetical protein (NCBI) compare
b0167 glnD -0.6 -2.8 PII uridylyl-transferase (NCBI) compare
b4590 ybfK -0.6 -0.8 hypothetical protein (RefSeq) compare
b1712 ihfA -0.6 -0.7 integration host factor subunit alpha (NCBI) compare
b2024 hisA -0.6 -1.9 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b0990 cspG -0.6 -1.1 DNA-binding transcriptional regulator (NCBI) compare
b2567 rnc -0.6 -0.3 ribonuclease III (NCBI) compare
b2576 srmB -0.6 -2.4 ATP-dependent RNA helicase (NCBI) compare
b2012 yeeD -0.6 -1.9 hypothetical protein (NCBI) compare
b1754 b1754 -0.6 -2.2 orf, hypothetical protein (VIMSS) compare
b1253 yciA -0.6 -2.0 predicted hydrolase (NCBI) compare
b2055 wcaE -0.6 -0.3 predicted glycosyl transferase (NCBI) compare
b1147 ymfL -0.6 -2.6 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b0200 gmhB -0.6 -1.0 hypothetical protein (NCBI) compare
b2314 dedD -0.6 -2.2 putative lipoprotein (VIMSS) compare
b4179 vacB -0.6 -3.8 putative enzyme (VIMSS) compare
b0500 ybbD -0.6 -0.3 hypothetical protein (RefSeq) compare
b1167 ymgC -0.6 -0.3 hypothetical protein (NCBI) compare
b1182 hlyE -0.6 -0.4 hemolysin E (VIMSS) compare
b2363 yfdT -0.6 -1.8 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4191 ulaR -0.6 -2.8 DNA-binding transcriptional dual regulator (NCBI) compare
b1690 ydiM -0.6 -1.7 predicted transporter (NCBI) compare
b3609 secB -0.6 -2.7 export protein SecB (NCBI) compare
b3387 dam -0.6 -4.7 DNA adenine methylase (NCBI) compare
b0014 dnaK -0.6 -1.8 molecular chaperone DnaK (NCBI) compare
b2343 b2343 -0.5 -1.2 orf, hypothetical protein (VIMSS) compare
b0018 mokC -0.5 -1.3 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b1400 paaY -0.5 -2.3 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b2820 recB -0.5 -0.8 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3159 yhbV -0.5 -4.4 orf, hypothetical protein (VIMSS) compare
b3500 gor -0.5 -1.4 glutathione reductase (NCBI) compare
b1176 minC -0.5 -1.9 septum formation inhibitor (NCBI) compare
b4177 purA -0.5 -1.3 adenylosuccinate synthetase (NCBI) compare
b1499 ydeO -0.5 -0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1257 yciE -0.5 -0.3 hypothetical protein (NCBI) compare
b2704 srlB -0.5 -2.9 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b1108 ycfP -0.5 -1.5 orf, hypothetical protein (VIMSS) compare
b1518 lsrG -0.5 -1.3 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1576 ydfD -0.5 -0.3 Qin prophage; predicted protein (NCBI) compare
b3164 pnp -0.5 -1.9 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3631 rfaG -0.5 -4.7 glucosyltransferase I (NCBI) compare
b3205 yhbJ -0.5 -2.6 hypothetical protein (NCBI) compare
b2017 yefM -0.5 -0.6 orf, hypothetical protein (VIMSS) compare
b2479 gcvR -0.5 -3.5 transcriptional regulation of gcv operon (VIMSS) compare
b2110 yehC -0.5 -1.2 predicted periplasmic pilin chaperone (NCBI) compare
b3204 ptsN -0.5 -3.0 sugar-specific enzyme IIA component of PTS (NCBI) compare
b1235 rssB -0.5 -3.7 response regulator of RpoS (NCBI) compare
b0279 yagM -0.5 -0.4 CP4-6 prophage; predicted protein (NCBI) compare
b1292 sapC -0.5 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0118 acnB -0.5 -0.6 aconitate hydratase (NCBI) compare
b1196 ycgY -0.5 -2.2 hypothetical protein (NCBI) compare
b4294 insA-7 -0.5 -1.7 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b4131 cadA -0.5 -4.5 lysine decarboxylase 1 (NCBI) compare
b2081 yegQ -0.5 -3.6 predicted peptidase (NCBI) compare
b0013 yaaI -0.5 -2.8 hypothetical protein (NCBI) compare
b1735 chbR -0.5 -2.4 DNA-binding transcriptional dual regulator (NCBI) compare
b4443 gcvB -0.5 -0.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b3630 rfaP -0.5 -3.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b4695 ykgT -0.5 -1.7 no description compare
b4414 tff -0.5 -1.5 unknown RNA (NCBI) compare
b0149 mrcB -0.5 -4.0 penicillin-binding protein 1b (NCBI) compare
b2822 recC -0.5 -2.9 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b4413 sokC -0.5 -1.4 regulatory antisense RNA (NCBI) compare
b0685 ybfE -0.5 -1.5 orf, hypothetical protein (VIMSS) compare
b2625 yfjI -0.5 -1.2 CP4-57 prophage; predicted protein (NCBI) compare
b1667 ydhR -0.5 -1.9 hypothetical protein (NCBI) compare
b2808 gcvA -0.5 -2.0 DNA-binding transcriptional dual regulator (NCBI) compare
b1627 rsxA -0.5 -1.9 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b3842 rfaH -0.5 -2.4 transcriptional activator RfaH (NCBI) compare
b2562 yfhL -0.5 -3.7 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b1192 ldcA -0.5 -1.2 L,D-carboxypeptidase A (NCBI) compare
b1148 ymfM -0.5 -2.5 e14 prophage; predicted protein (NCBI) compare
b2659 b2659 -0.5 -1.3 orf, hypothetical protein (VIMSS) compare
b1814 sdaA -0.5 -2.5 L-serine deaminase I (NCBI) compare
b1187 fadR -0.4 -2.3 fatty acid metabolism regulator (NCBI) compare
b0948 ycbY -0.4 -2.7 predicted methyltransferase (NCBI) compare
b0728 sucC -0.4 -3.0 succinyl-CoA synthetase subunit beta (NCBI) compare
b3930 menA -0.4 -4.0 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b2553 glnB -0.4 -2.0 regulatory protein P-II for glutamine synthetase (NCBI) compare
b1628 rsxB -0.4 -2.2 electron transport complex protein RnfB (NCBI) compare
b1818 manY -0.4 -1.9 mannose-specific enzyme IIC component of PTS (NCBI) compare
b1159 mcrA -0.4 -0.9 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b4497 yeeL -0.4 -0.8 no description compare
b1836 yebV -0.4 -1.5 orf, hypothetical protein (VIMSS) compare
b2450 yffS -0.4 -2.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b2853 ygeI -0.4 -0.2 hypothetical protein (NCBI) compare
b2528 iscA -0.4 -1.2 iron-sulfur cluster assembly protein (NCBI) compare
b4132 cadB -0.4 -3.9 predicted lysine/cadaverine transporter (NCBI) compare
b2297 pta -0.4 -0.5 phosphate acetyltransferase (NCBI) compare
b0049 apaH -0.4 -1.1 diadenosinetetraphosphatase (NCBI) compare
b2754 ygbF -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b0145 dksA -0.4 -1.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4048 yjbM -0.4 -0.9 hypothetical protein (NCBI) compare
b4655 ythA -0.4 -1.1 expressed protein (RefSeq) compare
b4178 yjeB -0.4 -2.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1968 yedV -0.4 -1.8 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b3977 tyrU -0.4 -0.5 tRNA-Tyr (NCBI) compare
b1273 yciN -0.4 -1.2 hypothetical protein (NCBI) compare
b2847 yqeI -0.4 -0.3 predicted transcriptional regulator (NCBI) compare
b1171 b1171 -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b1550 b1550 -0.4 -1.1 orf, hypothetical protein (VIMSS) compare
b4559 yjdO -0.4 -2.2 hypothetical protein (NCBI) compare
b2683 ygaH -0.4 -2.5 predicted inner membrane protein (NCBI) compare
b4679 yohP -0.4 -2.0 expressed protein (RefSeq) compare
b1441 ydcT -0.4 -1.6 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1855 lpxM -0.4 -2.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b0740 tolB -0.4 -1.3 translocation protein TolB precursor (NCBI) compare
b3935 priA -0.4 -1.2 primosome assembly protein PriA (NCBI) compare
b2393 nupC -0.4 -2.6 nucleoside (except guanosine) transporter (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in lb experiments