Experiment set42IT016 for Escherichia coli BW25113

Compare to:

LB Time24hr

200 most detrimental genes:

  gene name fitness t score description  
b1185 dsbB +1.2 5.2 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b2934 cmtB +1.1 1.0 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1332 ynaJ +1.1 1.4 predicted inner membrane protein (NCBI) compare
b4538 yoeF +1.0 3.2 hypothetical protein (RefSeq) compare
b3860 dsbA +0.8 7.4 periplasmic protein disulfide isomerase I (NCBI) compare
b2156 lysP +0.8 7.1 lysine transporter (NCBI) compare
b0730 mngR +0.8 1.6 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4603 rseX +0.8 1.0 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1954 dsrA +0.7 3.5 regulatory antisense RNA (NCBI) compare
b1830 prc +0.7 4.9 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2859 ygeO +0.7 2.3 hypothetical protein (RefSeq) compare
b1058 yceO +0.7 0.3 hypothetical protein (NCBI) compare
b2051 wcaH +0.7 1.6 GDP-mannose mannosyl hydrolase (VIMSS) compare
b1831 proQ +0.7 2.2 putative solute/DNA competence effector (NCBI) compare
b4480 hdfR +0.7 4.6 transcriptional regulator HdfR (NCBI) compare
b1573 ydfC +0.6 1.3 Qin prophage; predicted protein (RefSeq) compare
b4676 yoaK +0.6 0.6 expressed protein (RefSeq) compare
b4481 rffT +0.6 4.4 4-alpha-L-fucosyltransferase (NCBI) compare
b1195 ymgE +0.6 2.2 predicted inner membrane protein (NCBI) compare
b1183 umuD +0.6 1.8 DNA polymerase V, subunit D (NCBI) compare
b0630 lipB +0.6 1.0 protein of lipoate biosynthesis (VIMSS) compare
b1739 osmE +0.6 2.7 DNA-binding transcriptional activator (NCBI) compare
b3790 rffC +0.6 3.9 TDP-fucosamine acetyltransferase (RefSeq) compare
b1357 ydaS +0.6 1.5 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1686 ydiI +0.6 1.5 hypothetical protein (NCBI) compare
b0542 renD +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b3396 mrcA +0.5 5.2 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b4596 yciZ +0.5 0.9 hypothetical protein (RefSeq) compare
b1709 btuD +0.5 1.0 vitamin B12-transporter ATPase (NCBI) compare
b3786 rffE +0.5 4.6 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3764 yifE +0.5 2.9 hypothetical protein (NCBI) compare
b1742 b1742 +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b1348 lar +0.5 1.6 Rac prophage; restriction alleviation protein (NCBI) compare
b3794 rffM +0.5 3.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3784 rfe +0.5 4.4 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3961 oxyR +0.5 4.4 DNA-binding transcriptional dual regulator (NCBI) compare
b3787 rffD +0.5 4.1 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4000 hupA +0.5 2.9 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1251 yciI +0.5 2.2 orf, hypothetical protein (VIMSS) compare
b3163 nlpI +0.5 2.4 hypothetical protein (NCBI) compare
b4698 mgrR +0.4 2.6 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b4042 dgkA +0.4 0.3 diacylglycerol kinase (NCBI) compare
b0802 ybiJ +0.4 1.6 hypothetical protein (NCBI) compare
b4152 frdC +0.4 2.5 fumarate reductase subunit C (NCBI) compare
b2208 napF +0.4 2.4 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b1930 yedF +0.4 3.2 hypothetical protein (NCBI) compare
b2209 eco +0.4 0.9 ecotin precursor (NCBI) compare
b4218 ytfL +0.4 3.5 predicted inner membrane protein (NCBI) compare
b3791 rffA +0.4 3.4 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2741 rpoS +0.4 2.8 RNA polymerase sigma factor (NCBI) compare
b1233 ychJ +0.4 2.9 hypothetical protein (NCBI) compare
b4651 yibW +0.4 0.3 no description compare
b1764 selD +0.4 2.3 selenophosphate synthetase (NCBI) compare
b3623 rfaK +0.4 1.4 lipopolysaccharide core biosynthesis (NCBI) compare
b1765 ydjA +0.4 0.6 predicted oxidoreductase (NCBI) compare
b2572 rseA +0.4 2.4 anti-sigma factor (NCBI) compare
b4543 ypaA +0.4 2.4 predicted protein (NCBI) compare
b4376 osmY +0.4 1.9 periplasmic protein (NCBI) compare
b1520 b1520 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1724 ydiZ +0.4 1.4 hypothetical protein (NCBI) compare
b4551 yheV +0.4 1.9 hypothetical protein (NCBI) compare
b4631 ykgQ +0.4 1.4 no description compare
b1548 nohA +0.4 1.0 Qin prophage; predicted packaging protein (RefSeq) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b2004 yeeU +0.4 2.3 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b3147 yraM +0.4 3.3 hypothetical protein (NCBI) compare
b2699 recA +0.4 0.5 recombinase A (NCBI) compare
b1919 yedO +0.4 1.1 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b4157 yjeN +0.4 1.2 hypothetical protein (NCBI) compare
b4642 yoeD +0.4 1.2 no description compare
b0101 yacG +0.4 2.0 zinc-binding protein (NCBI) compare
b1305 pspB +0.4 1.7 phage shock protein B (NCBI) compare
b3181 greA +0.4 2.4 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3027 ygiZ +0.3 0.2 conserved inner membrane protein (NCBI) compare
b4609 ryfD +0.3 2.0 Novel sRNA, function unknown (RefSeq) compare
b2481 hyfA +0.3 2.4 hydrogenase 4 Fe-S subunit (VIMSS) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b1792 yeaO +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b1656 sodB +0.3 1.2 superoxide dismutase, Fe (NCBI) compare
b1152 ymfP +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b4460 araH +0.3 1.6 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1625 ydgT +0.3 2.1 predicted regulator (NCBI) compare
b0191 yaeJ +0.3 2.4 hypothetical protein (NCBI) compare
b4533 ynfO +0.3 1.2 Qin prophage; predicted protein (NCBI) compare
b1227 narI +0.3 1.9 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b1506 yneL +0.3 0.5 predicted transcriptional regulator (NCBI) compare
b4458 oxyS +0.3 0.5 global regulatory RNA (NCBI) compare
b2792 yqcC +0.3 1.1 hypothetical protein (NCBI) compare
b1462 b1462 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0183 rnhB +0.3 1.8 ribonuclease HII (NCBI) compare
b4614 sokA +0.3 1.3 no description compare
b3446 yrhB +0.3 2.2 hypothetical protein (NCBI) compare
b2279 nuoK +0.3 0.7 NADH dehydrogenase subunit K (NCBI) compare
b4571 wbbL +0.3 0.9 no description compare
b0209 yafD +0.3 2.3 hypothetical protein (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b3911 cpxA +0.3 2.6 two-component sensor protein (NCBI) compare
b4415 hokE +0.3 1.3 toxic polypeptide, small (NCBI) compare
b2587 kgtP +0.3 2.1 alpha-ketoglutarate transporter (NCBI) compare
b4418 sraB +0.3 0.6 unknown RNA (NCBI) compare
b2095 gatZ +0.3 1.9 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b0178 hlpA +0.3 1.5 periplasmic chaperone (NCBI) compare
b1684 sufA +0.3 1.3 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1503 ydeR +0.3 1.3 predicted fimbrial-like adhesin protein (NCBI) compare
b0713 ybgL +0.3 2.2 hypothetical protein (NCBI) compare
b1250 kch +0.3 2.0 voltage-gated potassium channel (NCBI) compare
b0798 ybiA +0.3 0.8 hypothetical protein (NCBI) compare
b0193 yaeF +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b1495 yddB +0.3 2.3 predicted porin protein (NCBI) compare
b1753 ynjA +0.3 1.1 hypothetical protein (NCBI) compare
b0849 grxA +0.3 1.2 glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI) compare
b1556 b1556 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b4558 yifL +0.3 1.8 predicted lipoprotein (NCBI) compare
b4441 glmY +0.3 0.9 sRNA activator of glmS mRNA (RefSeq) compare
b2997 hybO +0.3 2.1 hydrogenase 2, small subunit (NCBI) compare
b1511 ydeV +0.3 1.7 putative kinase (VIMSS) compare
b4573 insZ +0.3 1.4 no description compare
b0846 ybjK +0.3 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0323 yahI +0.3 1.7 predicted carbamate kinase-like protein (NCBI) compare
b3152 yraR +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b4585 sroB +0.3 0.6 Novel sRNA, function unknown (RefSeq) compare
b2916 argP +0.3 1.9 chromosome replication initiation inhibitor protein (NCBI) compare
b1768 ydjB +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b3291 mscL +0.3 2.0 large-conductance mechanosensitive channel (NCBI) compare
b1777 b1777 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b3613 yibP +0.3 0.8 putative membrane protein (VIMSS) compare
b4640 yoeG +0.3 1.0 no description compare
b2250 b2250 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b2586 yfiM +0.3 1.3 predicted protein (RefSeq) compare
b4656 yjhY +0.3 1.3 no description compare
b2264 menD +0.3 2.1 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1081 flgJ +0.3 1.9 flagellar biosynthesis protein FlgJ (NCBI) compare
b1287 yciW +0.3 1.4 putative oxidoreductase (VIMSS) compare
b1733 chbG +0.3 1.1 hypothetical protein (NCBI) compare
b2010 dacD +0.3 2.0 penicillin binding protein 6b (VIMSS) compare
b1948 fliP +0.3 1.6 flagellar biosynthesis protein P (NCBI) compare
b3236 mdh +0.3 2.0 malate dehydrogenase (NCBI) compare
b1977 asnT +0.3 1.2 tRNA-Asn (NCBI) compare
b3725 pstB +0.3 0.1 phosphate transporter subunit (NCBI) compare
b0933 ssuB +0.3 1.6 alkanesulfonate transporter subunit (NCBI) compare
b2535 csiE +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b0803 ybiI +0.3 1.6 hypothetical protein (NCBI) compare
b2544 yphB +0.3 1.7 hypothetical protein (NCBI) compare
b2014 yeeF +0.3 1.7 putative amino acid/amine transport protein (VIMSS) compare
b2165 yeiN +0.3 1.7 hypothetical protein (NCBI) compare
b3844 fre +0.3 1.6 NAD(P)H-flavin reductase (NCBI) compare
b0714 nei +0.3 1.5 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b4505 insX +0.3 0.4 predicted IS protein (RefSeq) compare
b2193 narP +0.3 1.7 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b3021 ygiT +0.2 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0226 dinJ +0.2 1.1 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1950 fliR +0.2 1.1 flagellar biosynthesis protein R (NCBI) compare
b0765 modC +0.2 1.7 molybdate transporter subunit (NCBI) compare
b3973 birA +0.2 0.9 biotin--protein ligase (NCBI) compare
b1789 yeaL +0.2 1.1 conserved inner membrane protein (NCBI) compare
b1847 yebF +0.2 0.5 predicted protein (RefSeq) compare
b0641 rlpB +0.2 1.1 minor lipoprotein (NCBI) compare
b4686 yshB +0.2 1.5 expressed protein (RefSeq) compare
b1758 b1758 +0.2 0.6 putative cytochrome oxidase (VIMSS) compare
b2096 gatY +0.2 1.8 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b3622 rfaL +0.2 1.3 O-antigen ligase (NCBI) compare
b1848 yebG +0.2 0.7 conserved protein regulated by LexA (NCBI) compare
b1151 ymfO +0.2 1.2 e14 prophage; conserved protein (RefSeq) compare
b3563 yiaB +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b2202 napC +0.2 1.4 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1663 mdtK +0.2 1.8 multidrug efflux protein NorM (NCBI) compare
b0920 ycbC +0.2 1.6 conserved inner membrane protein (NCBI) compare
b2730 hypE +0.2 1.8 carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq) compare
b3481 nikR +0.2 1.7 nickel responsive regulator (NCBI) compare
b2199 ccmC +0.2 1.7 heme exporter subunit (NCBI) compare
b0613 citG +0.2 1.7 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b4001 yjaH +0.2 1.5 hypothetical protein (NCBI) compare
b1557 cspB +0.2 1.0 Qin prophage; cold shock protein (NCBI) compare
b2700 ygaD +0.2 1.4 competence damage-inducible protein A (NCBI) compare
b2074 b2074 +0.2 1.3 putative membrane protein (VIMSS) compare
b2301 yfcF +0.2 1.3 predicted enzyme (NCBI) compare
b0564 appY +0.2 0.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3074 ygjH +0.2 1.6 hypothetical protein (NCBI) compare
b2645 yfjZ +0.2 1.4 CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b2200 ccmB +0.2 1.4 heme exporter subunit (NCBI) compare
b2900 yqfB +0.2 1.1 hypothetical protein (NCBI) compare
b1255 yciC +0.2 1.2 hypothetical protein (NCBI) compare
b1097 yceG +0.2 1.5 predicted aminodeoxychorismate lyase (NCBI) compare
b0207 dkgB +0.2 1.7 2,5-diketo-D-gluconate reductase B (NCBI) compare
b2486 hyfF +0.2 1.6 NADH dehydrogenase subunit N (NCBI) compare
b4312 fimB +0.2 1.9 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b0476 aes +0.2 1.7 acetyl esterase (NCBI) compare
b1225 narH +0.2 1.6 nitrate reductase 1, beta (Fe-S) subunit (NCBI) compare
b2426 ucpA +0.2 0.9 putative oxidoreductase (VIMSS) compare
b0427 yajR +0.2 2.0 putative transport protein (VIMSS) compare
b1342 ydaN +0.2 1.5 zinc transporter (NCBI) compare
b0447 ybaO +0.2 1.6 putative LRP-like transcriptional regulator (VIMSS) compare
b2809 b2809 +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b1035 ycdY +0.2 1.6 hypothetical protein (NCBI) compare
b0393 rdgC +0.2 1.9 recombination associated protein (NCBI) compare
b0192 nlpE +0.2 1.7 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b1823 cspC +0.2 0.9 stress protein, member of the CspA-family (NCBI) compare
b1248 b1248 +0.2 0.8 orf, hypothetical protein (VIMSS) compare
b1976 b1976 +0.2 0.9 orf, hypothetical protein (VIMSS) compare
b1461 ydcE +0.2 1.1 4-oxalocrotonate tautomerase (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in lb experiments