Experiment set42IT015 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time9hr

200 most detrimental genes:

  gene name fitness t score description  
b2934 cmtB +1.5 1.3 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1185 dsbB +1.4 7.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4603 rseX +1.0 1.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3860 dsbA +0.9 8.0 periplasmic protein disulfide isomerase I (NCBI) compare
b2846 yqeH +0.9 1.0 orf, hypothetical protein (VIMSS) compare
b1831 proQ +0.9 3.1 putative solute/DNA competence effector (NCBI) compare
b1476 fdnI +0.8 1.5 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b0630 lipB +0.8 1.5 protein of lipoate biosynthesis (VIMSS) compare
b1765 ydjA +0.8 1.4 predicted oxidoreductase (NCBI) compare
b2616 recN +0.6 5.2 recombination and repair protein (NCBI) compare
b4480 hdfR +0.6 4.4 transcriptional regulator HdfR (NCBI) compare
b1205 ychH +0.6 2.4 predicted inner membrane protein (NCBI) compare
b1143 ymfI +0.6 2.3 orf, hypothetical protein (VIMSS) compare
b4128 yjdK +0.6 0.6 hypothetical protein (NCBI) compare
b4538 yoeF +0.6 1.8 hypothetical protein (RefSeq) compare
b3021 ygiT +0.6 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2859 ygeO +0.6 1.8 hypothetical protein (RefSeq) compare
b2156 lysP +0.6 5.0 lysine transporter (NCBI) compare
b0798 ybiA +0.6 1.9 hypothetical protein (NCBI) compare
b1357 ydaS +0.5 2.0 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0730 mngR +0.5 1.1 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3181 greA +0.5 3.6 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3764 yifE +0.5 3.5 hypothetical protein (NCBI) compare
b1085 b1085 +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b3250 mreC +0.5 2.1 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b0542 renD +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b0439 lon +0.5 2.5 DNA-binding ATP-dependent protease La (NCBI) compare
b0617 citD +0.5 1.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b3889 yiiE +0.5 2.2 predicted transcriptional regulator (RefSeq) compare
b0802 ybiJ +0.5 2.1 hypothetical protein (NCBI) compare
b4551 yheV +0.5 2.6 hypothetical protein (NCBI) compare
b1753 ynjA +0.5 2.1 hypothetical protein (NCBI) compare
b1954 dsrA +0.5 2.0 regulatory antisense RNA (NCBI) compare
b4460 araH +0.5 2.0 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b2741 rpoS +0.5 3.4 RNA polymerase sigma factor (NCBI) compare
b2217 rcsB +0.5 3.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b4042 dgkA +0.5 0.3 diacylglycerol kinase (NCBI) compare
b2935 tktA +0.5 1.1 transketolase 1, thiamin-binding (NCBI) compare
b1823 cspC +0.5 1.9 stress protein, member of the CspA-family (NCBI) compare
b0191 yaeJ +0.4 3.4 hypothetical protein (NCBI) compare
b2587 kgtP +0.4 3.2 alpha-ketoglutarate transporter (NCBI) compare
b1195 ymgE +0.4 1.8 predicted inner membrane protein (NCBI) compare
b1553 ydfP +0.4 0.8 Qin prophage; conserved protein (NCBI) compare
b2673 nrdH +0.4 2.1 glutaredoxin-like protein (NCBI) compare
b3260 dusB +0.4 3.1 tRNA-dihydrouridine synthase B (NCBI) compare
b4519 icdC +0.4 2.1 no description compare
b3504 yhiS +0.4 0.4 hypothetical protein (RefSeq) compare
b2510 b2510 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b3961 oxyR +0.4 3.5 DNA-binding transcriptional dual regulator (NCBI) compare
b4239 treC +0.4 0.8 trehalose-6-P hydrolase (NCBI) compare
b2684 mprA +0.4 2.5 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3512 GadE +0.4 2.6 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b0183 rnhB +0.4 2.2 ribonuclease HII (NCBI) compare
b4045 yjbJ +0.4 1.8 predicted stress response protein (NCBI) compare
b2208 napF +0.4 2.1 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b0933 ssuB +0.4 1.7 alkanesulfonate transporter subunit (NCBI) compare
b1502 ydeQ +0.4 1.3 predicted fimbrial-like adhesin protein (NCBI) compare
b1058 yceO +0.4 0.2 hypothetical protein (NCBI) compare
b1461 ydcE +0.4 1.6 4-oxalocrotonate tautomerase (NCBI) compare
b1724 ydiZ +0.4 0.9 hypothetical protein (NCBI) compare
b4676 yoaK +0.4 0.4 expressed protein (RefSeq) compare
b3236 mdh +0.4 3.1 malate dehydrogenase (NCBI) compare
b1144 ymfJ +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b2617 smpA +0.4 1.4 small membrane protein A (VIMSS) compare
b0623 cspE +0.4 1.2 cold shock protein E (NCBI) compare
b1427 rimL +0.3 1.5 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b3259 prmA +0.3 2.6 ribosomal protein L11 methyltransferase (NCBI) compare
b1742 b1742 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1573 ydfC +0.3 1.0 Qin prophage; predicted protein (RefSeq) compare
b0247 ykfG +0.3 1.0 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0819 ybiS +0.3 2.8 hypothetical protein (NCBI) compare
b2279 nuoK +0.3 1.2 NADH dehydrogenase subunit K (NCBI) compare
b1581 rspA +0.3 1.0 predicted dehydratase (NCBI) compare
b4389 radA +0.3 2.7 predicted repair protein (NCBI) compare
b2645 yfjZ +0.3 1.9 CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b2212 alkB +0.3 1.7 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b1421 trg +0.3 2.4 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1387 maoC +0.3 2.1 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1351 racC +0.3 0.3 Rac prophage; predicted protein (NCBI) compare
b1438 ydcQ +0.3 1.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1134 ymfB +0.3 1.4 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b1625 ydgT +0.3 2.1 predicted regulator (NCBI) compare
b2535 csiE +0.3 2.8 orf, hypothetical protein (VIMSS) compare
b0388 aroL +0.3 2.1 shikimate kinase II (NCBI) compare
b0521 ybcF +0.3 2.2 predicted carbamate kinase (NCBI) compare
b4431 rprA +0.3 1.2 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b4492 ydbA +0.3 2.5 no description compare
b1790 yeaM +0.3 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b4646 yrdE +0.3 1.4 no description compare
b1478 adhP +0.3 1.9 alcohol dehydrogenase (VIMSS) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b1462 b1462 +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b0613 citG +0.3 1.8 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b2456 cchB +0.3 1.5 predicted carboxysome structural protein, ethanolamine utilization protein (NCBI) compare
b1930 yedF +0.3 1.5 hypothetical protein (NCBI) compare
b4171 miaA +0.3 1.3 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0226 dinJ +0.3 1.6 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4234 yjgA +0.3 1.3 hypothetical protein (NCBI) compare
b1339 abgR +0.3 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0888 trxB +0.3 1.2 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1858 znuC +0.3 2.3 high-affinity zinc transporter ATPase (NCBI) compare
b4571 wbbL +0.3 1.2 no description compare
b1709 btuD +0.3 0.5 vitamin B12-transporter ATPase (NCBI) compare
b4481 rffT +0.3 2.0 4-alpha-L-fucosyltransferase (NCBI) compare
b1408 b1408 +0.3 1.8 probable enzyme (VIMSS) compare
b1077 flgF +0.3 2.4 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b1179 ycgL +0.3 1.2 conserved protein (NCBI) compare
b1848 yebG +0.3 0.8 conserved protein regulated by LexA (NCBI) compare
b1977 asnT +0.3 1.4 tRNA-Asn (NCBI) compare
b0991 ymcE +0.3 1.9 cold shock gene (NCBI) compare
b4363 yjjB +0.3 1.9 conserved inner membrane protein (RefSeq) compare
b2572 rseA +0.3 2.1 anti-sigma factor (NCBI) compare
b2782 chpA +0.3 1.5 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b0393 rdgC +0.3 2.2 recombination associated protein (NCBI) compare
b2622 intA +0.3 2.0 CP4-57 prophage; integrase (NCBI) compare
b1081 flgJ +0.3 1.9 flagellar biosynthesis protein FlgJ (NCBI) compare
b0218 yafU +0.3 1.0 predicted inner membrane protein (RefSeq) compare
b4585 sroB +0.3 0.7 Novel sRNA, function unknown (RefSeq) compare
b1217 chaB +0.3 1.0 cation transport regulator (NCBI) compare
b1238 tdk +0.3 1.7 thymidine kinase (NCBI) compare
b1233 ychJ +0.3 1.6 hypothetical protein (NCBI) compare
b3627 rfaI +0.3 2.2 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2435 amiA +0.3 1.9 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1936 intG +0.3 0.5 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b3875 ompL +0.3 1.2 predicted outer membrane porin L (NCBI) compare
b2096 gatY +0.3 1.7 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4596 yciZ +0.3 0.8 hypothetical protein (RefSeq) compare
b1113 ycfS +0.3 1.7 hypothetical protein (NCBI) compare
b3787 rffD +0.3 2.2 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3462 ftsX +0.3 1.7 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0108 ppdD +0.3 1.4 predicted major pilin subunit (NCBI) compare
b1053 mdtG +0.3 1.5 predicted drug efflux system (NCBI) compare
b1399 paaX +0.3 1.3 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b3264 envR +0.3 1.6 DNA-binding transcriptional regulator (NCBI) compare
b1811 yoaH +0.3 1.1 hypothetical protein (NCBI) compare
b4387 ytjB +0.3 1.9 hypothetical protein (NCBI) compare
b4376 osmY +0.3 1.3 periplasmic protein (NCBI) compare
b4280 yjhC +0.3 1.9 putative dehydrogenase (VIMSS) compare
b4515 ybgT +0.3 0.8 hypothetical protein (NCBI) compare
b1365 ynaK +0.3 1.9 Rac prophage; conserved protein (NCBI) compare
b1849 purT +0.2 1.6 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b2911 ssrS +0.2 0.8 6S regulatory RNA (NCBI) compare
b2792 yqcC +0.2 0.8 hypothetical protein (NCBI) compare
b4218 ytfL +0.2 1.8 predicted inner membrane protein (NCBI) compare
b2486 hyfF +0.2 1.8 NADH dehydrogenase subunit N (NCBI) compare
b3207 yrbL +0.2 1.6 hypothetical protein (NCBI) compare
b1729 ydjN +0.2 1.4 predicted transporter (NCBI) compare
b3206 npr +0.2 1.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1610 tus +0.2 1.4 DNA replication terminus site-binding protein (NCBI) compare
b0101 yacG +0.2 0.7 zinc-binding protein (NCBI) compare
b0548 ninE +0.2 1.2 DLP12 prophage; conserved protein (NCBI) compare
b4686 yshB +0.2 1.3 expressed protein (RefSeq) compare
b3786 rffE +0.2 2.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2250 b2250 +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b4388 serB +0.2 2.0 3-phosphoserine phosphatase (NCBI) compare
b3293 yhdN +0.2 1.5 hypothetical protein (NCBI) compare
b4527 b1354 +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b1961 dcm +0.2 0.5 DNA cytosine methylase (NCBI) compare
b2633 yfjQ +0.2 1.9 CP4-57 prophage; predicted protein (NCBI) compare
b2261 menC +0.2 1.7 O-succinylbenzoate synthase (NCBI) compare
b3120 yhaB +0.2 0.6 orf, hypothetical protein (VIMSS) compare
b1434 ydcN +0.2 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1763 topB +0.2 1.5 DNA topoisomerase III (NCBI) compare
b1847 yebF +0.2 0.5 predicted protein (RefSeq) compare
b1919 yedO +0.2 0.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b4532 yncN +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b1959 yedA +0.2 1.2 predicted inner membrane protein (NCBI) compare
b4692 oweE +0.2 1.1 no description compare
b4699 fnrS +0.2 0.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1784 yeaH +0.2 1.7 yeaH component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
b2993 hybD +0.2 1.4 predicted maturation element for hydrogenase 2 (NCBI) compare
b1524 yneH +0.2 0.4 predicted glutaminase (NCBI) compare
b2743 pcm +0.2 1.3 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2193 narP +0.2 1.5 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b0545 ybcL +0.2 1.8 DLP12 prophage; predicted kinase inhibitor (NCBI) compare
b1453 ansP +0.2 1.6 L-asparagine permease (VIMSS) compare
b3156 yhbS +0.2 1.9 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3037 ygiB +0.2 1.4 conserved outer membrane protein (RefSeq) compare
b1739 osmE +0.2 1.0 DNA-binding transcriptional activator (NCBI) compare
b1390 paaC +0.2 1.5 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b1035 ycdY +0.2 1.7 hypothetical protein (NCBI) compare
b1418 cybB +0.2 1.0 cytochrome b(561) (VIMSS) compare
b1414 ydcF +0.2 1.6 hypothetical protein (NCBI) compare
b2682 ygaZ +0.2 1.4 predicted transporter (NCBI) compare
b0848 ybjM +0.2 1.4 predicted inner membrane protein (NCBI) compare
b2488 hyfH +0.2 1.0 hydrogenase 4, Fe-S subunit (NCBI) compare
b2103 thiD +0.2 1.5 phosphomethylpyrimidine kinase (NCBI) compare
b4687 shoB +0.2 1.1 toxic membrane protein (RefSeq) compare
b3844 fre +0.2 1.3 NAD(P)H-flavin reductase (NCBI) compare
b4369 leuP +0.2 1.2 tRNA-Leu (NCBI) compare
b1415 aldA +0.2 1.4 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b2368 emrK +0.2 1.7 EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component (NCBI) compare
b2281 nuoI +0.2 0.9 NADH dehydrogenase subunit I (NCBI) compare
b3794 rffM +0.2 1.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3507 dctR +0.2 2.0 protein involved in metabolism of C4-dicarboxylates (Katherine Huang) compare
b0957 ompA +0.2 1.1 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b0660 ybeZ +0.2 1.5 predicted protein with nucleoside triphosphate hydrolase domain (RefSeq) compare
b4325 yjiC +0.2 1.2 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms