Experiment set42IT007 for Escherichia coli BW25113

Compare to:

LB regrowth after 48hrs

200 most important genes:

  gene name fitness t score description  
b4053 alr -8.4 -5.8 alanine racemase (NCBI) compare
b4237 nrdG -6.0 -4.1 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b0949 uup -5.7 -16.9 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1850 eda -5.7 -3.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b4238 nrdD -5.6 -3.8 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.9 -4.6 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -4.9 -13.9 glucose-6-phosphate isomerase (NCBI) compare
b0386 proC -3.4 -7.1 pyrroline-5-carboxylate reductase (NCBI) compare
b1334 fnr -3.4 -3.2 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b0737 tolQ -3.1 -2.1 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3781 trxA -3.1 -6.8 thioredoxin 1 (VIMSS) compare
b0688 pgm -2.9 -5.6 phosphoglucomutase (NCBI) compare
b1123 potD -2.8 -12.2 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b4402 yjjY -2.8 -1.6 hypothetical protein (NCBI) compare
b2620 smpB -2.8 -2.5 SsrA-binding protein (NCBI) compare
b3052 rfaE -2.8 -8.4 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1124 potC -2.7 -13.3 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b0145 dksA -2.7 -5.7 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1126 potA -2.6 -14.9 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b2912 ygfA -2.6 -4.7 putative ligase (VIMSS) compare
b1125 potB -2.6 -7.1 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3977 tyrU -2.6 -1.8 tRNA-Tyr (NCBI) compare
b0902 pflA -2.5 -11.3 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b0243 proA -2.4 -15.8 gamma-glutamyl phosphate reductase (NCBI) compare
b3390 aroK -2.4 -4.4 shikimate kinase I (NCBI) compare
b4218 ytfL -2.4 -15.2 predicted inner membrane protein (NCBI) compare
b3620 rfaF -2.4 -9.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3619 rfaD -2.4 -5.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0739 tolA -2.3 -3.4 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2576 srmB -2.3 -6.1 ATP-dependent RNA helicase (NCBI) compare
b1236 galU -2.2 -7.4 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3621 rfaC -2.2 -6.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0222 lpcA -2.2 -4.5 phosphoheptose isomerase (NCBI) compare
b0053 surA -2.1 -11.0 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0912 ihfB -2.1 -4.5 integration host factor subunit beta (NCBI) compare
b3839 tatC -2.1 -8.4 TatABCE protein translocation system subunit (NCBI) compare
b3229 sspA -2.0 -3.6 stringent starvation protein A (NCBI) compare
b3930 menA -2.0 -16.7 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1292 sapC -2.0 -4.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0242 proB -1.9 -7.9 gamma-glutamyl kinase (NCBI) compare
b0142 folK -1.9 -1.3 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b3916 pfkA -1.9 -4.3 6-phosphofructokinase (NCBI) compare
b0741 pal -1.9 -4.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2330 yfcB -1.9 -2.0 putative adenine-specific methylase (VIMSS) compare
b2017 yefM -1.8 -1.6 orf, hypothetical protein (VIMSS) compare
b3162 deaD -1.8 -6.8 inducible ATP-independent RNA helicase (VIMSS) compare
b2329 aroC -1.7 -7.8 chorismate synthase (NCBI) compare
b3842 rfaH -1.6 -8.3 transcriptional activator RfaH (NCBI) compare
b0200 gmhB -1.6 -2.1 hypothetical protein (NCBI) compare
b0908 aroA -1.6 -11.0 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3405 ompR -1.6 -6.1 osmolarity response regulator (NCBI) compare
b3493 pitA -1.6 -10.0 phosphate transporter, low-affinity (NCBI) compare
b3183 obgE -1.6 -2.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0167 glnD -1.6 -6.8 PII uridylyl-transferase (NCBI) compare
b0903 pflB -1.6 -11.6 pyruvate formate lyase I (NCBI) compare
b2528 iscA -1.5 -2.6 iron-sulfur cluster assembly protein (NCBI) compare
b1291 sapD -1.5 -2.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1049 mdoH -1.5 -10.4 glucosyltransferase MdoH (NCBI) compare
b1677 lpp -1.5 -4.0 murein lipoprotein (NCBI) compare
b2830 nudH -1.5 -2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b0237 pepD -1.5 -10.5 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b4593 ymgI -1.5 -1.4 hypothetical protein (RefSeq) compare
b3281 aroE -1.5 -12.8 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3389 aroB -1.5 -11.4 3-dehydroquinate synthase (NCBI) compare
b3868 glnG -1.4 -8.7 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0740 tolB -1.4 -3.6 translocation protein TolB precursor (NCBI) compare
b1290 sapF -1.4 -2.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3706 trmE -1.4 -5.2 tRNA modification GTPase (NCBI) compare
b2318 truA -1.3 -4.0 tRNA pseudouridine synthase A (NCBI) compare
b0232 yafN -1.3 -1.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0728 sucC -1.3 -8.6 succinyl-CoA synthetase subunit beta (NCBI) compare
b0051 ksgA -1.3 -6.2 dimethyladenosine transferase (NCBI) compare
b0683 fur -1.3 -1.9 ferric uptake regulator (NCBI) compare
b0437 clpP -1.3 -7.0 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b4177 purA -1.3 -2.5 adenylosuccinate synthetase (NCBI) compare
b1048 mdoG -1.3 -7.4 glucan biosynthesis protein, periplasmic (NCBI) compare
b1861 ruvA -1.3 -1.3 Holliday junction DNA helicase motor protein (NCBI) compare
b2569 lepA -1.2 -5.5 GTP-binding protein LepA (NCBI) compare
b3741 gidA -1.2 -2.8 glucose-inhibited division protein A (NCBI) compare
b1693 aroD -1.2 -6.1 3-dehydroquinate dehydratase (NCBI) compare
b1804 rnd -1.2 -6.0 ribonuclease D (NCBI) compare
b0729 sucD -1.2 -4.8 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2659 b2659 -1.2 -2.1 orf, hypothetical protein (VIMSS) compare
b1865 nudB -1.2 -2.5 dATP pyrophosphohydrolase (NCBI) compare
b3631 rfaG -1.2 -10.3 glucosyltransferase I (NCBI) compare
b0948 ycbY -1.2 -4.2 predicted methyltransferase (NCBI) compare
b0438 clpX -1.1 -6.3 ATP-dependent protease ATP-binding subunit (NCBI) compare
b1863 ruvC -1.1 -2.0 Holliday junction resolvase (NCBI) compare
b3158 yhbU -1.1 -8.3 predicted peptidase (collagenase-like) (NCBI) compare
b4375 prfC -1.1 -2.4 peptide chain release factor 3 (NCBI) compare
b4131 cadA -1.1 -10.1 lysine decarboxylase 1 (NCBI) compare
b0014 dnaK -1.1 -3.7 molecular chaperone DnaK (NCBI) compare
b3630 rfaP -1.1 -8.6 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b4179 vacB -1.1 -6.7 putative enzyme (VIMSS) compare
b4202 rpsR -1.1 -2.1 30S ribosomal protein S18 (NCBI) compare
b0439 lon -1.1 -4.1 DNA-binding ATP-dependent protease La (NCBI) compare
b1286 rnb -1.1 -2.6 exoribonuclease II (NCBI) compare
b1187 fadR -1.0 -2.8 fatty acid metabolism regulator (NCBI) compare
b4414 tff -1.0 -2.1 unknown RNA (NCBI) compare
b1258 yciF -1.0 -1.1 hypothetical protein (NCBI) compare
b2808 gcvA -1.0 -3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b4191 ulaR -1.0 -5.3 DNA-binding transcriptional dual regulator (NCBI) compare
b2947 gshB -1.0 -5.5 glutathione synthetase (NCBI) compare
b3164 pnp -1.0 -3.4 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4132 cadB -1.0 -8.9 predicted lysine/cadaverine transporter (NCBI) compare
b0990 cspG -1.0 -1.5 DNA-binding transcriptional regulator (NCBI) compare
b2501 ppk -1.0 -7.4 polyphosphate kinase (NCBI) compare
b0890 ftsK -1.0 -3.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3636 rpmG -1.0 -2.4 50S ribosomal protein L33 (NCBI) compare
b3159 yhbV -1.0 -8.6 orf, hypothetical protein (VIMSS) compare
b2297 pta -1.0 -0.8 phosphate acetyltransferase (NCBI) compare
b3341 rpsG -1.0 -1.4 30S ribosomal protein S7 (NCBI) compare
b3613 yibP -1.0 -1.5 putative membrane protein (VIMSS) compare
b1860 ruvB -1.0 -1.3 Holliday junction DNA helicase B (NCBI) compare
b3387 dam -1.0 -7.6 DNA adenine methylase (NCBI) compare
b0082 mraW -1.0 -3.2 S-adenosyl-methyltransferase (NCBI) compare
b3261 fis -1.0 -0.6 DNA-binding protein Fis (NCBI) compare
b0738 tolR -0.9 -1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3500 gor -0.9 -2.1 glutathione reductase (NCBI) compare
b4171 miaA -0.9 -3.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4178 yjeB -0.9 -5.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b0080 fruR -0.9 -5.3 DNA-binding transcriptional dual regulator (NCBI) compare
b1192 ldcA -0.9 -2.2 L,D-carboxypeptidase A (NCBI) compare
b1347 ydaC -0.9 -0.5 Rac prophage; predicted protein (NCBI) compare
b4685 yrbN -0.9 -2.6 expressed protein (RefSeq) compare
b2393 nupC -0.9 -5.9 nucleoside (except guanosine) transporter (NCBI) compare
b3247 cafA -0.9 -7.5 bundles of cytoplasmic filaments (VIMSS) compare
b2820 recB -0.9 -1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b4153 frdB -0.9 -6.6 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b2512 yfgL -0.9 -4.0 protein assembly complex, lipoprotein component (NCBI) compare
b2363 yfdT -0.9 -3.3 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b3035 tolC -0.9 -3.6 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4154 frdA -0.9 -7.7 fumarate reductase (NCBI) compare
b1855 lpxM -0.9 -3.3 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b3806 cyaA -0.9 -5.5 adenylate cyclase (NCBI) compare
b1182 hlyE -0.8 -0.5 hemolysin E (VIMSS) compare
b3180 yhbY -0.8 -3.6 predicted RNA-binding protein (NCBI) compare
b4163 glyV -0.8 -2.2 tRNA-Gly (NCBI) compare
b1235 rssB -0.8 -5.2 response regulator of RpoS (NCBI) compare
b2894 xerD -0.8 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b1505 ydeT -0.8 -1.4 hypothetical protein (RefSeq) compare
b1088 yceD -0.8 -4.0 hypothetical protein (NCBI) compare
b2498 upp -0.8 -2.2 uracil phosphoribosyltransferase (VIMSS) compare
b2642 yfjW -0.8 -1.6 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b0149 mrcB -0.8 -6.7 penicillin-binding protein 1b (NCBI) compare
b3792 wzxE -0.8 -5.0 O-antigen translocase (NCBI) compare
b3157 yhbT -0.8 -3.5 predicted lipid carrier protein (NCBI) compare
b1551 b1551 -0.8 -0.6 orf, hypothetical protein (VIMSS) compare
b0603 ybdO -0.8 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1712 ihfA -0.8 -0.8 integration host factor subunit alpha (NCBI) compare
b1658 purR -0.8 -4.1 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1253 yciA -0.8 -2.9 predicted hydrolase (NCBI) compare
b2168 fruK -0.7 -0.8 1-phosphofructokinase (NCBI) compare
b4201 priB -0.7 -1.8 primosomal replication protein N (NCBI) compare
b4462 ygaQ -0.7 -1.9 hypothetical protein (RefSeq) compare
b3169 nusA -0.7 -1.9 transcription elongation factor NusA (NCBI) compare
b1161 ycgX -0.7 -1.1 hypothetical protein (NCBI) compare
b3204 ptsN -0.7 -3.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2625 yfjI -0.7 -1.6 CP4-57 prophage; predicted protein (NCBI) compare
b4552 yrhC -0.7 -1.2 no description compare
b2081 yegQ -0.7 -5.3 predicted peptidase (NCBI) compare
b0436 tig -0.7 -5.2 trigger factor (NCBI) compare
b1497 b1497 -0.7 -0.4 putative enzyme (VIMSS) compare
b3863 polA -0.7 -0.8 DNA polymerase I (NCBI) compare
b2572 rseA -0.7 -2.5 anti-sigma factor (NCBI) compare
b2055 wcaE -0.7 -0.3 predicted glycosyl transferase (NCBI) compare
b3253 yhdH -0.7 -5.3 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b4702 mgtL -0.7 -1.9 regulatory leader peptide for mgtA (NCBI) compare
b3911 cpxA -0.7 -3.4 two-component sensor protein (NCBI) compare
b4066 yjcF -0.7 -0.6 hypothetical protein (NCBI) compare
b2553 glnB -0.7 -2.9 regulatory protein P-II for glutamine synthetase (NCBI) compare
b1001 yccE -0.7 -0.3 hypothetical protein (NCBI) compare
b2683 ygaH -0.7 -3.2 predicted inner membrane protein (NCBI) compare
b2562 yfhL -0.7 -5.3 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b4384 deoD -0.7 -5.4 purine nucleoside phosphorylase (NCBI) compare
b2479 gcvR -0.7 -4.1 transcriptional regulation of gcv operon (VIMSS) compare
b4413 sokC -0.7 -1.9 regulatory antisense RNA (NCBI) compare
b3404 envZ -0.7 -4.7 osmolarity sensor protein (NCBI) compare
b3871 bipA -0.6 -5.6 GTP-binding protein (NCBI) compare
b3375 yhfR -0.6 -4.7 putative transcriptional regulator (VIMSS) compare
b4133 cadC -0.6 -5.9 DNA-binding transcriptional activator (NCBI) compare
b0500 ybbD -0.6 -0.3 hypothetical protein (RefSeq) compare
b3257 yhdT -0.6 -4.5 conserved inner membrane protein (NCBI) compare
b3728 pstS -0.6 -2.9 phosphate transporter subunit (NCBI) compare
b1628 rsxB -0.6 -2.2 electron transport complex protein RnfB (NCBI) compare
b1167 ymgC -0.6 -0.3 hypothetical protein (NCBI) compare
b3408 feoA -0.6 -5.3 ferrous iron transport protein A (NCBI) compare
b3131 agaR -0.6 -3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3115 tdcD -0.6 -5.8 propionate kinase/acetate kinase C, anaerobic (RefSeq) compare
b2083 yegZ -0.6 -2.0 orf, hypothetical protein (VIMSS) compare
b2343 b2343 -0.6 -1.9 orf, hypothetical protein (VIMSS) compare
b1853 yebK -0.6 -2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b3237 argR -0.6 -3.2 arginine repressor (NCBI) compare
b3465 yhhF -0.6 -4.5 predicted methyltransferase (NCBI) compare
b1499 ydeO -0.6 -0.3 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1576 ydfD -0.6 -0.3 Qin prophage; predicted protein (NCBI) compare
b0357 yaiN -0.6 -1.7 putative alpha helix chain (VIMSS) compare
b1241 adhE -0.6 -4.3 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b2352 yfdI -0.6 -0.8 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b0226 dinJ -0.6 -2.9 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment