Experiment set42IT006 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time48hr

200 most detrimental genes:

  gene name fitness t score description  
b3979 thrT +1.5 0.5 tRNA-Thr (NCBI) compare
b1185 dsbB +1.3 8.1 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1361 ydaW +1.2 1.1 orf, hypothetical protein (VIMSS) compare
b3860 dsbA +1.1 8.9 periplasmic protein disulfide isomerase I (NCBI) compare
b4699 fnrS +0.9 1.5 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b0630 lipB +0.9 1.6 protein of lipoate biosynthesis (VIMSS) compare
b1831 proQ +0.8 3.4 putative solute/DNA competence effector (NCBI) compare
b0798 ybiA +0.8 2.4 hypothetical protein (NCBI) compare
b1332 ynaJ +0.8 0.6 predicted inner membrane protein (NCBI) compare
b1205 ychH +0.7 1.9 predicted inner membrane protein (NCBI) compare
b0730 mngR +0.7 1.5 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4538 yoeF +0.7 2.1 hypothetical protein (RefSeq) compare
b1195 ymgE +0.6 2.4 predicted inner membrane protein (NCBI) compare
b4505 insX +0.6 1.9 predicted IS protein (RefSeq) compare
b3181 greA +0.6 4.0 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1357 ydaS +0.6 2.2 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1742 b1742 +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b1085 b1085 +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b4642 yoeD +0.5 1.3 no description compare
b0929 ompF +0.5 3.6 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b0542 renD +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b1418 cybB +0.5 1.3 cytochrome b(561) (VIMSS) compare
b3027 ygiZ +0.5 0.3 conserved inner membrane protein (NCBI) compare
b2156 lysP +0.5 4.2 lysine transporter (NCBI) compare
b1480 sra +0.5 1.4 30S ribosomal subunit protein S22 (NCBI) compare
b4218 ytfL +0.5 3.7 predicted inner membrane protein (NCBI) compare
b1408 b1408 +0.4 3.0 probable enzyme (VIMSS) compare
b3512 GadE +0.4 2.9 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1709 btuD +0.4 0.9 vitamin B12-transporter ATPase (NCBI) compare
b2051 wcaH +0.4 1.7 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0464 acrR +0.4 3.9 DNA-binding transcriptional repressor (NCBI) compare
b1238 tdk +0.4 3.1 thymidine kinase (NCBI) compare
b1476 fdnI +0.4 0.7 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b3961 oxyR +0.4 3.7 DNA-binding transcriptional dual regulator (NCBI) compare
b4596 yciZ +0.4 1.3 hypothetical protein (RefSeq) compare
b1179 ycgL +0.4 1.7 conserved protein (NCBI) compare
b4603 rseX +0.4 0.5 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b4345 mcrC +0.4 0.8 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1152 ymfP +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b0388 aroL +0.4 2.4 shikimate kinase II (NCBI) compare
b0218 yafU +0.4 1.9 predicted inner membrane protein (RefSeq) compare
b1448 yncA +0.4 1.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1462 b1462 +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b1382 ynbE +0.4 1.2 predicted lipoprotein (NCBI) compare
b2433 b2433 +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b2435 amiA +0.4 3.1 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1461 ydcE +0.4 2.0 4-oxalocrotonate tautomerase (NCBI) compare
b0521 ybcF +0.4 1.9 predicted carbamate kinase (NCBI) compare
b4387 ytjB +0.4 2.4 hypothetical protein (NCBI) compare
b3120 yhaB +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b0852 rimK +0.4 2.3 ribosomal protein S6 modification protein (NCBI) compare
b1882 cheY +0.4 0.4 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1610 tus +0.4 1.8 DNA replication terminus site-binding protein (NCBI) compare
b1753 ynjA +0.4 1.6 hypothetical protein (NCBI) compare
b1442 ydcU +0.4 2.2 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1342 ydaN +0.4 2.3 zinc transporter (NCBI) compare
b2708 gutQ +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b1387 maoC +0.4 2.2 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b0191 yaeJ +0.4 2.6 hypothetical protein (NCBI) compare
b4551 yheV +0.4 1.8 hypothetical protein (NCBI) compare
b3129 sohA +0.3 1.7 predicted regulator (NCBI) compare
b1954 dsrA +0.3 0.9 regulatory antisense RNA (NCBI) compare
b4640 yoeG +0.3 1.3 no description compare
b2935 tktA +0.3 0.8 transketolase 1, thiamin-binding (NCBI) compare
b2217 rcsB +0.3 2.5 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b1151 ymfO +0.3 1.7 e14 prophage; conserved protein (RefSeq) compare
b3889 yiiE +0.3 1.4 predicted transcriptional regulator (RefSeq) compare
b3026 qseC +0.3 1.4 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b1811 yoaH +0.3 1.7 hypothetical protein (NCBI) compare
b0183 rnhB +0.3 2.3 ribonuclease HII (NCBI) compare
b2915 yqfE +0.3 2.2 hypothetical protein (RefSeq) compare
b1421 trg +0.3 2.4 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1739 osmE +0.3 1.4 DNA-binding transcriptional activator (NCBI) compare
b2504 yfgG +0.3 0.7 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b0418 pgpA +0.3 2.4 phosphatidylglycerophosphatase A (NCBI) compare
b1858 znuC +0.3 2.5 high-affinity zinc transporter ATPase (NCBI) compare
b4492 ydbA +0.3 2.7 no description compare
b1440 ydcS +0.3 2.5 predicted spermidine/putrescine transporter subunit (NCBI) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b1153 ymfQ +0.3 2.0 e14 prophage; conserved protein (NCBI) compare
b0803 ybiI +0.3 1.6 hypothetical protein (NCBI) compare
b1415 aldA +0.3 2.1 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b0393 rdgC +0.3 2.3 recombination associated protein (NCBI) compare
b1530 marR +0.3 1.1 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b2399 yfeD +0.3 1.8 predicted DNA-binding transcriptional regulator (RefSeq) compare
b4381 deoC +0.3 2.0 deoxyribose-phosphate aldolase (NCBI) compare
b0645 ybeR +0.3 2.4 hypothetical protein (NCBI) compare
b1161 ycgX +0.3 0.6 hypothetical protein (NCBI) compare
b1380 ldhA +0.3 1.6 D-lactate dehydrogenase (NCBI) compare
b0848 ybjM +0.3 1.8 predicted inner membrane protein (NCBI) compare
b1410 ynbC +0.3 2.2 predicted hydrolase (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b4686 yshB +0.3 1.2 expressed protein (RefSeq) compare
b1426 ydcH +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b1388 paaA +0.3 2.1 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b0821 ybiU +0.3 2.1 hypothetical protein (NCBI) compare
b1467 narY +0.3 1.8 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b2250 b2250 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b0696 kdpC +0.3 1.0 potassium-transporting ATPase subunit C (NCBI) compare
b1453 ansP +0.3 1.7 L-asparagine permease (VIMSS) compare
b1411 ynbD +0.3 2.2 predicted phosphatase, inner membrane protein (NCBI) compare
b2445 yffN +0.3 1.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b2684 mprA +0.3 1.7 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b3082 ygjM +0.3 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1463 nhoA +0.3 2.2 N-hydroxyarylamine O-acetyltransferase (NCBI) compare
b1143 ymfI +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b2096 gatY +0.3 2.1 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b4146 yjeK +0.3 0.3 predicted lysine aminomutase (NCBI) compare
b2587 kgtP +0.3 1.8 alpha-ketoglutarate transporter (NCBI) compare
b1427 rimL +0.3 1.0 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b0209 yafD +0.3 2.0 hypothetical protein (NCBI) compare
b3555 yiaG +0.3 1.3 predicted transcriptional regulator (NCBI) compare
b2846 yqeH +0.3 0.3 orf, hypothetical protein (VIMSS) compare
b1930 yedF +0.3 2.0 hypothetical protein (NCBI) compare
b1439 ydcR +0.3 1.7 fused predicted DNA-binding transcriptional regulator/predicted amino transferase (NCBI) compare
b1625 ydgT +0.3 1.9 predicted regulator (NCBI) compare
b4376 osmY +0.3 1.9 periplasmic protein (NCBI) compare
b1764 selD +0.3 1.6 selenophosphate synthetase (NCBI) compare
b2010 dacD +0.3 1.9 penicillin binding protein 6b (VIMSS) compare
b2356 yfdM +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b2859 ygeO +0.3 0.7 hypothetical protein (RefSeq) compare
b0846 ybjK +0.3 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b3875 ompL +0.3 1.2 predicted outer membrane porin L (NCBI) compare
b2672 ygaM +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b4484 cpxP +0.3 2.1 periplasmic protein combats stress (NCBI) compare
b1824 yobF +0.3 1.0 hypothetical protein (NCBI) compare
b3100 yqjK +0.3 1.4 hypothetical protein (NCBI) compare
b4701 sokX +0.3 0.3 sok-related sRNA, function unknown (RefSeq) compare
b3037 ygiB +0.3 1.8 conserved outer membrane protein (RefSeq) compare
b3630 rfaP +0.3 2.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2258 b2258 +0.3 0.7 putative transport/receptor protein (VIMSS) compare
b1154 ycfK +0.3 2.0 e14 prophage; predicted protein (NCBI) compare
b0600 ybdL +0.3 2.0 putative aminotransferase (NCBI) compare
b2622 intA +0.3 1.8 CP4-57 prophage; integrase (NCBI) compare
b1768 ydjB +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2682 ygaZ +0.2 1.5 predicted transporter (NCBI) compare
b1515 lsrD +0.2 1.2 AI2 transporter (NCBI) compare
b2023 hisH +0.2 1.6 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b4363 yjjB +0.2 1.4 conserved inner membrane protein (RefSeq) compare
b2052 fcl +0.2 1.5 bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI) compare
b1977 asnT +0.2 1.0 tRNA-Asn (NCBI) compare
b2398 yfeC +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b2208 napF +0.2 1.3 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b2209 eco +0.2 0.8 ecotin precursor (NCBI) compare
b1399 paaX +0.2 1.7 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b1038 csgF +0.2 1.0 predicted transport protein (NCBI) compare
b4529 ydbJ +0.2 0.5 hypothetical protein (NCBI) compare
b3236 mdh +0.2 2.0 malate dehydrogenase (NCBI) compare
b2940 yqgC +0.2 1.7 hypothetical protein (NCBI) compare
b1838 pphA +0.2 1.7 protein phosphatase 1 modulates phosphoproteins, signals protein misfolding (VIMSS) compare
b0617 citD +0.2 0.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1414 ydcF +0.2 1.7 hypothetical protein (NCBI) compare
b0269 yagF +0.2 1.8 CP4-6 prophage; predicted dehydratase (NCBI) compare
b2741 rpoS +0.2 1.5 RNA polymerase sigma factor (NCBI) compare
b1349 recT +0.2 1.9 Rac prophage; recombination and repair protein (NCBI) compare
b1917 yecC +0.2 1.5 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2743 pcm +0.2 1.6 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2419 yfeK +0.2 1.0 hypothetical protein (NCBI) compare
b1394 paaG +0.2 1.0 enoyl-CoA hydratase (NCBI) compare
b0407 yajC +0.2 0.6 preprotein translocase subunit YajC (NCBI) compare
b1487 ddpA +0.2 1.7 D-ala-D-a la transporter subunit (NCBI) compare
b2294 b2294 +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b3481 nikR +0.2 1.6 nickel responsive regulator (NCBI) compare
b3775 ppiC +0.2 1.6 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b1434 ydcN +0.2 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b2947 gshB +0.2 1.3 glutathione synthetase (NCBI) compare
b2221 atoD +0.2 1.3 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b3625 rfaY +0.2 1.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1731 cedA +0.2 1.3 cell division modulator (RefSeq) compare
b1115 ycfT +0.2 1.7 predicted inner membrane protein (NCBI) compare
b1044 ymdA +0.2 1.3 hypothetical protein (NCBI) compare
b2264 menD +0.2 1.9 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1604 ydgH +0.2 1.2 hypothetical protein (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b4573 insZ +0.2 1.3 no description compare
b3248 yhdE +0.2 1.8 Maf-like protein (NCBI) compare
b3021 ygiT +0.2 0.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1444 ydcW +0.2 1.5 medium chain aldehyde dehydrogenase (NCBI) compare
b1407 ydbD +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b4158 yjeO +0.2 1.5 conserved inner membrane protein (NCBI) compare
b4157 yjeN +0.2 0.6 hypothetical protein (NCBI) compare
b0933 ssuB +0.2 1.3 alkanesulfonate transporter subunit (NCBI) compare
b3263 yhdU +0.2 1.7 predicted membrane protein (NCBI) compare
b4325 yjiC +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b4431 rprA +0.2 0.8 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b2876 b2876 +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b1676 pykF +0.2 1.1 pyruvate kinase (NCBI) compare
b3631 rfaG +0.2 2.0 glucosyltransferase I (NCBI) compare
b2782 chpA +0.2 1.5 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b1707 ydiV +0.2 1.1 hypothetical protein (NCBI) compare
b1469 narU +0.2 1.4 nitrate/nitrite transporter (NCBI) compare
b1524 yneH +0.2 0.4 predicted glutaminase (NCBI) compare
b1706 ydiU +0.2 1.3 hypothetical protein (NCBI) compare
b3698 yidB +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b1659 ydhB +0.2 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1138 ymfE +0.2 0.6 e14 prophage; predicted inner membrane protein (NCBI) compare
b3727 pstC +0.2 1.6 phosphate transporter subunit (NCBI) compare
b4693 aaaE +0.2 1.5 no description compare
b1520 b1520 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b1424 mdoD +0.2 1.5 glucan biosynthesis protein, periplasmic (RefSeq) compare


Specific Phenotypes

For 2 genes in this experiment

For stress Carbenicillin disodium salt in Escherichia coli BW25113

For stress Carbenicillin disodium salt across organisms