Experiment set42IT005 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time24hr

200 most detrimental genes:

  gene name fitness t score description  
b3979 thrT +1.5 0.5 tRNA-Thr (NCBI) compare
b2934 cmtB +1.1 1.0 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1185 dsbB +1.1 6.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA +1.0 8.5 periplasmic protein disulfide isomerase I (NCBI) compare
b0630 lipB +0.9 1.6 protein of lipoate biosynthesis (VIMSS) compare
b4596 yciZ +0.8 1.6 hypothetical protein (RefSeq) compare
b1709 btuD +0.8 1.7 vitamin B12-transporter ATPase (NCBI) compare
b1765 ydjA +0.8 1.3 predicted oxidoreductase (NCBI) compare
b1476 fdnI +0.7 1.3 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b0798 ybiA +0.7 2.4 hypothetical protein (NCBI) compare
b0998 torD +0.7 1.7 chaperone protein TorD (NCBI) compare
b1831 proQ +0.7 2.7 putative solute/DNA competence effector (NCBI) compare
b2156 lysP +0.7 6.3 lysine transporter (NCBI) compare
b0730 mngR +0.7 1.5 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4603 rseX +0.7 0.9 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b4538 yoeF +0.7 1.9 hypothetical protein (RefSeq) compare
b3790 rffC +0.7 5.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b3163 nlpI +0.7 4.1 hypothetical protein (NCBI) compare
b3791 rffA +0.6 5.0 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1160 elbA +0.6 1.1 hypothetical protein (NCBI) compare
b1954 dsrA +0.6 2.6 regulatory antisense RNA (NCBI) compare
b1085 b1085 +0.6 2.1 orf, hypothetical protein (VIMSS) compare
b3794 rffM +0.6 4.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1830 prc +0.6 3.7 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1195 ymgE +0.6 1.8 predicted inner membrane protein (NCBI) compare
b4481 rffT +0.6 4.1 4-alpha-L-fucosyltransferase (NCBI) compare
b0114 aceE +0.5 1.2 pyruvate dehydrogenase subunit E1 (NCBI) compare
b3786 rffE +0.5 4.7 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3787 rffD +0.5 4.6 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b4480 hdfR +0.5 3.6 transcriptional regulator HdfR (NCBI) compare
b3396 mrcA +0.5 5.0 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b3784 rfe +0.5 4.7 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1573 ydfC +0.5 0.7 Qin prophage; predicted protein (RefSeq) compare
b0542 renD +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b1305 pspB +0.5 2.1 phage shock protein B (NCBI) compare
b1348 lar +0.5 1.2 Rac prophage; restriction alleviation protein (NCBI) compare
b2587 kgtP +0.5 2.9 alpha-ketoglutarate transporter (NCBI) compare
b3147 yraM +0.4 3.9 hypothetical protein (NCBI) compare
b4573 insZ +0.4 2.9 no description compare
b0843 ybjH +0.4 1.0 hypothetical protein (NCBI) compare
b1179 ycgL +0.4 2.2 conserved protein (NCBI) compare
b4000 hupA +0.4 2.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1261 trpB +0.4 2.4 tryptophan synthase subunit beta (NCBI) compare
b4551 yheV +0.4 2.1 hypothetical protein (NCBI) compare
b2741 rpoS +0.4 3.0 RNA polymerase sigma factor (NCBI) compare
b4676 yoaK +0.4 0.3 expressed protein (RefSeq) compare
b0933 ssuB +0.4 1.9 alkanesulfonate transporter subunit (NCBI) compare
b4609 ryfD +0.4 1.8 Novel sRNA, function unknown (RefSeq) compare
b2208 napF +0.4 2.2 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b0183 rnhB +0.4 2.7 ribonuclease HII (NCBI) compare
b2250 b2250 +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b4369 leuP +0.4 1.5 tRNA-Leu (NCBI) compare
b2209 eco +0.4 1.1 ecotin precursor (NCBI) compare
b1233 ychJ +0.4 2.3 hypothetical protein (NCBI) compare
b1530 marR +0.4 1.8 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b4218 ytfL +0.4 2.9 predicted inner membrane protein (NCBI) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b1790 yeaM +0.4 2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b3961 oxyR +0.4 3.1 DNA-binding transcriptional dual regulator (NCBI) compare
b4699 fnrS +0.4 0.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1951 rcsA +0.4 0.9 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b1524 yneH +0.4 0.7 predicted glutaminase (NCBI) compare
b2510 b2510 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1644 b1644 +0.4 1.8 putative membrane protein (VIMSS) compare
b3021 ygiT +0.4 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1822 rrmA +0.4 1.5 23S rRNA m1G745 methyltransferase (NCBI) compare
b2212 alkB +0.4 1.9 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b0388 aroL +0.4 2.6 shikimate kinase II (NCBI) compare
b1823 cspC +0.4 1.3 stress protein, member of the CspA-family (NCBI) compare
b3483 yhhH +0.4 0.2 orf, hypothetical protein (VIMSS) compare
b4157 yjeN +0.3 1.0 hypothetical protein (NCBI) compare
b0191 yaeJ +0.3 2.6 hypothetical protein (NCBI) compare
b1724 ydiZ +0.3 1.5 hypothetical protein (NCBI) compare
b0218 yafU +0.3 1.6 predicted inner membrane protein (RefSeq) compare
b1961 dcm +0.3 1.1 DNA cytosine methylase (NCBI) compare
b4045 yjbJ +0.3 1.5 predicted stress response protein (NCBI) compare
b1480 sra +0.3 1.1 30S ribosomal subunit protein S22 (NCBI) compare
b2291 yfbR +0.3 1.4 hypothetical protein (NCBI) compare
b4360 yjjA +0.3 1.7 putative glycoprotein/receptor (VIMSS) compare
b1663 mdtK +0.3 2.3 multidrug efflux protein NorM (NCBI) compare
b1753 ynjA +0.3 1.4 hypothetical protein (NCBI) compare
b4460 araH +0.3 1.6 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1726 yniB +0.3 1.4 predicted inner membrane protein (NCBI) compare
b0942 ycbU +0.3 2.6 predicted fimbrial-like adhesin protein (NCBI) compare
b3027 ygiZ +0.3 0.2 conserved inner membrane protein (NCBI) compare
b3100 yqjK +0.3 1.7 hypothetical protein (NCBI) compare
b1566 flxA +0.3 1.1 Qin prophage; predicted protein (NCBI) compare
b0683 fur +0.3 0.6 ferric uptake regulator (NCBI) compare
b4387 ytjB +0.3 2.1 hypothetical protein (NCBI) compare
b2264 menD +0.3 2.7 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b2916 argP +0.3 2.4 chromosome replication initiation inhibitor protein (NCBI) compare
b1768 ydjB +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b1792 yeaO +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2853 ygeI +0.3 0.2 hypothetical protein (NCBI) compare
b4642 yoeD +0.3 1.2 no description compare
b1138 ymfE +0.3 0.8 e14 prophage; predicted inner membrane protein (NCBI) compare
b1332 ynaJ +0.3 0.3 predicted inner membrane protein (NCBI) compare
b2572 rseA +0.3 2.0 anti-sigma factor (NCBI) compare
b1930 yedF +0.3 2.2 hypothetical protein (NCBI) compare
b3911 cpxA +0.3 2.7 two-component sensor protein (NCBI) compare
b1506 yneL +0.3 0.7 predicted transcriptional regulator (NCBI) compare
b2051 wcaH +0.3 0.7 GDP-mannose mannosyl hydrolase (VIMSS) compare
b3973 birA +0.3 1.2 biotin--protein ligase (NCBI) compare
b2809 b2809 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b0696 kdpC +0.3 1.0 potassium-transporting ATPase subunit C (NCBI) compare
b4513 kdpF +0.3 0.5 potassium ion accessory transporter subunit (NCBI) compare
b1357 ydaS +0.3 1.1 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1344 ydaO +0.3 1.2 predicted C32 tRNA thiolase (NCBI) compare
b1919 yedO +0.3 1.1 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b4686 yshB +0.3 1.8 expressed protein (RefSeq) compare
b4411 ecnB +0.3 1.8 entericidin B membrane lipoprotein (NCBI) compare
b1143 ymfI +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b4519 icdC +0.3 1.5 no description compare
b4614 sokA +0.3 1.3 no description compare
b2362 yfdS +0.3 1.1 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2433 b2433 +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b3555 yiaG +0.3 1.7 predicted transcriptional regulator (NCBI) compare
b3962 udhA +0.3 2.3 putative oxidoreductase (VIMSS) compare
b1252 tonB +0.3 1.1 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1949 fliQ +0.3 1.2 flagellar biosynthesis protein Q (NCBI) compare
b0802 ybiJ +0.3 1.4 hypothetical protein (NCBI) compare
b2096 gatY +0.3 1.8 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2778 ygcG +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b4618 tisB +0.3 1.7 lexA-regulated toxic peptide (RefSeq) compare
b1959 yedA +0.3 1.4 predicted inner membrane protein (NCBI) compare
b1408 b1408 +0.3 1.7 probable enzyme (VIMSS) compare
b1238 tdk +0.3 1.8 thymidine kinase (NCBI) compare
b1113 ycfS +0.3 1.6 hypothetical protein (NCBI) compare
b1299 puuR +0.3 1.7 DNA-binding transcriptional repressor (NCBI) compare
b0852 rimK +0.3 1.9 ribosomal protein S6 modification protein (NCBI) compare
b2500 purN +0.3 2.0 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2782 chpA +0.3 1.8 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b3481 nikR +0.3 2.0 nickel responsive regulator (NCBI) compare
b4381 deoC +0.3 1.8 deoxyribose-phosphate aldolase (NCBI) compare
b1114 mfd +0.3 2.0 transcription-repair coupling factor (NCBI) compare
b2014 yeeF +0.3 2.0 putative amino acid/amine transport protein (VIMSS) compare
b2486 hyfF +0.3 1.8 NADH dehydrogenase subunit N (NCBI) compare
b2261 menC +0.3 1.9 O-succinylbenzoate synthase (NCBI) compare
b2071 yegJ +0.3 0.9 hypothetical protein (NCBI) compare
b1904 yecR +0.3 1.6 hypothetical protein (NCBI) compare
b2195 ccmG +0.3 1.0 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b0571 cusR +0.3 2.0 DNA-binding response regulator in two-component regulatory system with CusS (NCBI) compare
b1249 cls +0.3 1.7 cardiolipin synthetase (NCBI) compare
b3443 yrhA +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b0521 ybcF +0.3 1.5 predicted carbamate kinase (NCBI) compare
b1739 osmE +0.3 0.8 DNA-binding transcriptional activator (NCBI) compare
b4522 yciX_1 +0.3 0.8 hypothetical protein (NCBI) compare
b3006 exbB +0.3 2.1 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2103 thiD +0.2 2.1 phosphomethylpyrimidine kinase (NCBI) compare
b4363 yjjB +0.2 1.5 conserved inner membrane protein (RefSeq) compare
b3250 mreC +0.2 0.8 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b4234 yjgA +0.2 1.3 hypothetical protein (NCBI) compare
b0645 ybeR +0.2 1.9 hypothetical protein (NCBI) compare
b2266 elaB +0.2 0.9 hypothetical protein (NCBI) compare
b1611 fumC +0.2 1.6 fumarate hydratase (NCBI) compare
b1874 cutC +0.2 0.8 copper homeostasis protein (NCBI) compare
b3775 ppiC +0.2 1.4 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b3099 yqjE +0.2 1.5 conserved inner membrane protein (NCBI) compare
b1656 sodB +0.2 0.8 superoxide dismutase, Fe (NCBI) compare
b1698 ydiR +0.2 1.5 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b4646 yrdE +0.2 1.1 no description compare
b1686 ydiI +0.2 0.7 hypothetical protein (NCBI) compare
b4388 serB +0.2 1.8 3-phosphoserine phosphatase (NCBI) compare
b4017 arpA +0.2 1.4 regulator of acetyl CoA synthetase (NCBI) compare
b0946 ycbW +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b2481 hyfA +0.2 1.4 hydrogenase 4 Fe-S subunit (VIMSS) compare
b2217 rcsB +0.2 1.9 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3476 nikA +0.2 2.0 nickel transporter subunit (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b0208 yafC +0.2 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1339 abgR +0.2 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1058 yceO +0.2 0.1 hypothetical protein (NCBI) compare
b3764 yifE +0.2 1.4 hypothetical protein (NCBI) compare
b2199 ccmC +0.2 1.4 heme exporter subunit (NCBI) compare
b0393 rdgC +0.2 2.0 recombination associated protein (NCBI) compare
b1907 tyrP +0.2 1.1 tyrosine transporter (NCBI) compare
b3362 yhfG +0.2 1.6 hypothetical protein (NCBI) compare
b3478 nikC +0.2 2.1 nickel transporter subunit (NCBI) compare
b3236 mdh +0.2 1.7 malate dehydrogenase (NCBI) compare
b0209 yafD +0.2 1.9 hypothetical protein (NCBI) compare
b4377 yjjU +0.2 1.9 predicted esterase (NCBI) compare
b0996 torC +0.2 1.7 trimethylamine N-oxide (TMAO) reductase I, cytochrome c-type subunit (NCBI) compare
b1205 ychH +0.2 0.8 predicted inner membrane protein (NCBI) compare
b1553 ydfP +0.2 0.5 Qin prophage; conserved protein (NCBI) compare
b2399 yfeD +0.2 1.4 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1035 ycdY +0.2 1.5 hypothetical protein (NCBI) compare
b2997 hybO +0.2 1.8 hydrogenase 2, small subunit (NCBI) compare
b4541 yehK +0.2 1.1 hypothetical protein (NCBI) compare
b0193 yaeF +0.2 1.8 orf, hypothetical protein (VIMSS) compare
b2294 b2294 +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b0836 yliH +0.2 1.0 hypothetical protein (NCBI) compare
b1045 ymdB +0.2 1.4 hypothetical protein (NCBI) compare
b0447 ybaO +0.2 1.7 putative LRP-like transcriptional regulator (VIMSS) compare
b3752 rbsK +0.2 1.9 ribokinase (NCBI) compare
b1729 ydjN +0.2 1.2 predicted transporter (NCBI) compare
b1260 trpA +0.2 1.7 tryptophan synthase subunit alpha (NCBI) compare
b2292 yfbS +0.2 1.8 predicted transporter (NCBI) compare
b3672 ivbL +0.2 1.2 ilvB operon leader peptide (NCBI) compare
b1335 ogt +0.2 1.2 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b0226 dinJ +0.2 0.7 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms