Experiment set42IT002 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time8hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -6.4 -16.6 alanine racemase (NCBI) compare
b4238 nrdD -4.9 -12.4 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4237 nrdG -4.8 -8.6 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4041 plsB -4.4 -5.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -3.1 -14.5 glucose-6-phosphate isomerase (NCBI) compare
b1334 fnr -2.9 -3.3 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1850 eda -2.0 -4.5 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0386 proC -1.9 -6.3 pyrroline-5-carboxylate reductase (NCBI) compare
b0949 uup -1.8 -11.6 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0243 proA -1.7 -11.0 gamma-glutamyl phosphate reductase (NCBI) compare
b2330 yfcB -1.7 -1.5 putative adenine-specific methylase (VIMSS) compare
b0739 tolA -1.7 -3.5 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0741 pal -1.6 -4.5 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2830 nudH -1.6 -2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b2168 fruK -1.6 -1.5 1-phosphofructokinase (NCBI) compare
b0688 pgm -1.5 -4.6 phosphoglucomutase (NCBI) compare
b0902 pflA -1.4 -7.8 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b4552 yrhC -1.4 -1.6 no description compare
b1361 ydaW -1.4 -0.8 orf, hypothetical protein (VIMSS) compare
b0908 aroA -1.4 -9.4 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b1125 potB -1.4 -6.0 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3781 trxA -1.3 -6.7 thioredoxin 1 (VIMSS) compare
b0737 tolQ -1.3 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1123 potD -1.3 -7.1 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b2528 iscA -1.3 -3.5 iron-sulfur cluster assembly protein (NCBI) compare
b4202 rpsR -1.3 -2.4 30S ribosomal protein S18 (NCBI) compare
b3389 aroB -1.3 -10.4 3-dehydroquinate synthase (NCBI) compare
b2620 smpB -1.3 -1.7 SsrA-binding protein (NCBI) compare
b1236 galU -1.3 -5.2 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1049 mdoH -1.3 -9.7 glucosyltransferase MdoH (NCBI) compare
b0242 proB -1.2 -6.4 gamma-glutamyl kinase (NCBI) compare
b1124 potC -1.2 -5.8 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1292 sapC -1.2 -3.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4402 yjjY -1.2 -1.2 hypothetical protein (NCBI) compare
b2329 aroC -1.2 -6.0 chorismate synthase (NCBI) compare
b3281 aroE -1.1 -10.1 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b1860 ruvB -1.1 -1.6 Holliday junction DNA helicase B (NCBI) compare
b1048 mdoG -1.1 -6.6 glucan biosynthesis protein, periplasmic (NCBI) compare
b2297 pta -1.1 -0.7 phosphate acetyltransferase (NCBI) compare
b3390 aroK -1.1 -2.4 shikimate kinase I (NCBI) compare
b3162 deaD -1.1 -5.0 inducible ATP-independent RNA helicase (VIMSS) compare
b0200 gmhB -1.1 -1.5 hypothetical protein (NCBI) compare
b3868 glnG -1.1 -6.8 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b1126 potA -1.1 -4.5 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b1551 b1551 -1.0 -0.6 orf, hypothetical protein (VIMSS) compare
b0903 pflB -1.0 -7.7 pyruvate formate lyase I (NCBI) compare
b3842 rfaH -1.0 -6.0 transcriptional activator RfaH (NCBI) compare
b2912 ygfA -1.0 -2.7 putative ligase (VIMSS) compare
b1291 sapD -0.9 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3493 pitA -0.9 -6.3 phosphate transporter, low-affinity (NCBI) compare
b2318 truA -0.9 -2.4 tRNA pseudouridine synthase A (NCBI) compare
b1693 aroD -0.9 -4.0 3-dehydroquinate dehydratase (NCBI) compare
b3052 rfaE -0.8 -4.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3405 ompR -0.8 -3.7 osmolarity response regulator (NCBI) compare
b3183 obgE -0.8 -1.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3619 rfaD -0.8 -3.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3035 tolC -0.8 -3.6 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1677 lpp -0.8 -2.4 murein lipoprotein (NCBI) compare
b0728 sucC -0.8 -5.2 succinyl-CoA synthetase subunit beta (NCBI) compare
b2846 yqeH -0.8 -0.7 orf, hypothetical protein (VIMSS) compare
b0990 cspG -0.8 -1.5 DNA-binding transcriptional regulator (NCBI) compare
b4414 tff -0.8 -2.6 unknown RNA (NCBI) compare
b0167 glnD -0.8 -4.2 PII uridylyl-transferase (NCBI) compare
b3631 rfaG -0.8 -7.2 glucosyltransferase I (NCBI) compare
b2894 xerD -0.8 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b0237 pepD -0.8 -5.6 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b0729 sucD -0.8 -4.3 succinyl-CoA synthetase subunit alpha (NCBI) compare
b2501 ppk -0.8 -5.9 polyphosphate kinase (NCBI) compare
b1863 ruvC -0.8 -1.3 Holliday junction resolvase (NCBI) compare
b4375 prfC -0.8 -2.3 peptide chain release factor 3 (NCBI) compare
b0912 ihfB -0.8 -1.6 integration host factor subunit beta (NCBI) compare
b4593 ymgI -0.8 -0.9 hypothetical protein (RefSeq) compare
b0438 clpX -0.7 -4.4 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0222 lpcA -0.7 -2.3 phosphoheptose isomerase (NCBI) compare
b0053 surA -0.7 -4.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0232 yafN -0.7 -0.7 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2659 b2659 -0.7 -2.7 orf, hypothetical protein (VIMSS) compare
b0303 ykgI -0.7 -0.8 orf, hypothetical protein (VIMSS) compare
b0603 ybdO -0.7 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1882 cheY -0.7 -0.6 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3806 cyaA -0.7 -3.9 adenylate cyclase (NCBI) compare
b2820 recB -0.7 -1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3159 yhbV -0.7 -6.2 orf, hypothetical protein (VIMSS) compare
b0740 tolB -0.7 -2.1 translocation protein TolB precursor (NCBI) compare
b0560 nohB -0.7 -3.3 DLP12 prophage; DNA packaging protein (NCBI) compare
b0234 yafP -0.7 -1.7 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4462 ygaQ -0.7 -1.8 hypothetical protein (RefSeq) compare
b0014 dnaK -0.7 -2.7 molecular chaperone DnaK (NCBI) compare
b4179 vacB -0.7 -4.6 putative enzyme (VIMSS) compare
b4535 yniD -0.7 -1.3 predicted protein (RefSeq) compare
b3204 ptsN -0.7 -3.9 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3728 pstS -0.7 -3.0 phosphate transporter subunit (NCBI) compare
b0738 tolR -0.6 -1.2 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2369 evgA -0.6 -3.1 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b2652 ileY -0.6 -0.8 tRNA-Ile (NCBI) compare
b3630 rfaP -0.6 -5.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1712 ihfA -0.6 -0.7 integration host factor subunit alpha (NCBI) compare
b1235 rssB -0.6 -4.8 response regulator of RpoS (NCBI) compare
b3158 yhbU -0.6 -4.8 predicted peptidase (collagenase-like) (NCBI) compare
b2081 yegQ -0.6 -4.9 predicted peptidase (NCBI) compare
b1429 tehA -0.6 -0.8 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b0500 ybbD -0.6 -0.3 hypothetical protein (RefSeq) compare
b3811 xerC -0.6 -0.7 site-specific tyrosine recombinase XerC (NCBI) compare
b2055 wcaE -0.6 -0.3 predicted glycosyl transferase (NCBI) compare
b2576 srmB -0.6 -2.3 ATP-dependent RNA helicase (NCBI) compare
b1001 yccE -0.6 -0.3 hypothetical protein (NCBI) compare
b4131 cadA -0.6 -5.5 lysine decarboxylase 1 (NCBI) compare
b2567 rnc -0.6 -0.3 ribonuclease III (NCBI) compare
b0145 dksA -0.6 -1.5 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1497 b1497 -0.6 -0.3 putative enzyme (VIMSS) compare
b2822 recC -0.6 -3.5 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b4132 cadB -0.6 -5.2 predicted lysine/cadaverine transporter (NCBI) compare
b0890 ftsK -0.6 -2.2 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b2450 yffS -0.6 -1.4 CPZ-55 prophage; predicted protein (RefSeq) compare
b3930 menA -0.6 -5.0 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b2024 hisA -0.6 -2.1 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b3620 rfaF -0.6 -3.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1861 ruvA -0.5 -0.7 Holliday junction DNA helicase motor protein (NCBI) compare
b4685 yrbN -0.5 -1.8 expressed protein (RefSeq) compare
b1165 ymgA -0.5 -0.3 hypothetical protein (NCBI) compare
b1171 b1171 -0.5 -1.8 orf, hypothetical protein (VIMSS) compare
b1167 ymgC -0.5 -0.3 hypothetical protein (NCBI) compare
b2625 yfjI -0.5 -1.3 CP4-57 prophage; predicted protein (NCBI) compare
b1358 ydaT -0.5 -1.4 Rac prophage; predicted protein (NCBI) compare
b3115 tdcD -0.5 -5.0 propionate kinase/acetate kinase C, anaerobic (RefSeq) compare
b1865 nudB -0.5 -1.1 dATP pyrophosphohydrolase (NCBI) compare
b0051 ksgA -0.5 -2.9 dimethyladenosine transferase (NCBI) compare
b4497 yeeL -0.5 -0.9 no description compare
b3465 yhhF -0.5 -3.7 predicted methyltransferase (NCBI) compare
b1257 yciE -0.5 -0.3 hypothetical protein (NCBI) compare
b1804 rnd -0.5 -3.0 ribonuclease D (NCBI) compare
b4702 mgtL -0.5 -1.7 regulatory leader peptide for mgtA (NCBI) compare
b3157 yhbT -0.5 -2.2 predicted lipid carrier protein (NCBI) compare
b3210 arcB -0.5 -3.1 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b2814 metZ -0.5 -1.3 tRNA-Met (NCBI) compare
b1576 ydfD -0.5 -0.2 Qin prophage; predicted protein (NCBI) compare
b3229 sspA -0.5 -1.4 stringent starvation protein A (NCBI) compare
b3761 trpT -0.5 -1.4 tRNA-Trp (NCBI) compare
b2553 glnB -0.5 -2.3 regulatory protein P-II for glutamine synthetase (NCBI) compare
b0437 clpP -0.5 -2.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b0118 acnB -0.5 -0.7 aconitate hydratase (NCBI) compare
b3636 rpmG -0.5 -1.3 50S ribosomal protein L33 (NCBI) compare
b3180 yhbY -0.5 -2.7 predicted RNA-binding protein (NCBI) compare
b2285 nuoE -0.5 -1.5 NADH dehydrogenase subunit E (NCBI) compare
b4048 yjbM -0.5 -1.1 hypothetical protein (NCBI) compare
b1853 yebK -0.5 -2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2319 usg -0.5 -2.4 hypothetical protein (NCBI) compare
b1129 phoQ -0.5 -1.1 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1187 fadR -0.5 -2.1 fatty acid metabolism regulator (NCBI) compare
b0279 yagM -0.5 -0.4 CP4-6 prophage; predicted protein (NCBI) compare
b1499 ydeO -0.5 -0.2 predicted DNA-binding transcriptional acfivator (NCBI) compare
b0658 ybeX -0.5 -0.5 predicteed ion transport (NCBI) compare
b4443 gcvB -0.5 -0.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b4699 fnrS -0.5 -0.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b0114 aceE -0.5 -0.9 pyruvate dehydrogenase subunit E1 (NCBI) compare
b2479 gcvR -0.5 -2.8 transcriptional regulation of gcv operon (VIMSS) compare
b0082 mraW -0.5 -2.3 S-adenosyl-methyltransferase (NCBI) compare
b2363 yfdT -0.5 -1.9 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b1147 ymfL -0.5 -2.0 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b0881 clpS -0.5 -1.4 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b4191 ulaR -0.5 -2.0 DNA-binding transcriptional dual regulator (NCBI) compare
b2683 ygaH -0.5 -2.6 predicted inner membrane protein (NCBI) compare
b1632 rsxE -0.5 -2.9 NADH-ubiquinone oxidoreductase (NCBI) compare
b2847 yqeI -0.5 -0.3 predicted transcriptional regulator (NCBI) compare
b0149 mrcB -0.4 -3.8 penicillin-binding protein 1b (NCBI) compare
b2853 ygeI -0.4 -0.2 hypothetical protein (NCBI) compare
b1685 ydiH -0.4 -1.3 predicted protein (RefSeq) compare
b3387 dam -0.4 -3.6 DNA adenine methylase (NCBI) compare
b4453 ldrD -0.4 -1.8 toxic polypeptide, small (NCBI) compare
b1921 fliZ -0.4 -1.6 orf, hypothetical protein (VIMSS) compare
b3609 secB -0.4 -2.0 export protein SecB (NCBI) compare
b1278 pgpB -0.4 -1.9 phosphatidylglycerophosphatase B (NCBI) compare
b1593 b1593 -0.4 -2.4 orf, hypothetical protein (VIMSS) compare
b3119 tdcR -0.4 -2.1 threonine dehydratase operon activator protein (VIMSS) compare
b0357 yaiN -0.4 -0.9 putative alpha helix chain (VIMSS) compare
b3612 gpmI -0.4 -3.9 phosphoglyceromutase (NCBI) compare
b2343 b2343 -0.4 -1.5 orf, hypothetical protein (VIMSS) compare
b1778 yeaA -0.4 -1.8 methionine sulfoxide reductase B (NCBI) compare
b1104 ycfL -0.4 -1.1 hypothetical protein (NCBI) compare
b1150 ymfR -0.4 -0.8 e14 prophage; predicted protein (NCBI) compare
b2393 nupC -0.4 -3.1 nucleoside (except guanosine) transporter (NCBI) compare
b4426 isrA -0.4 -2.0 unknown RNA (NCBI) compare
b4201 priB -0.4 -1.3 primosomal replication protein N (NCBI) compare
b2562 yfhL -0.4 -3.4 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b2948 yqgE -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b2498 upp -0.4 -1.3 uracil phosphoribosyltransferase (VIMSS) compare
b1253 yciA -0.4 -2.5 predicted hydrolase (NCBI) compare
b2111 yehD -0.4 -2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b3239 yhcO -0.4 -2.2 predicted barnase inhibitor (NCBI) compare
b4133 cadC -0.4 -3.7 DNA-binding transcriptional activator (NCBI) compare
b3247 cafA -0.4 -3.5 bundles of cytoplasmic filaments (VIMSS) compare
b1968 yedV -0.4 -1.8 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b1594 dgsA -0.4 -1.7 DNA-binding transcriptional repressor (NCBI) compare
b2808 gcvA -0.4 -1.7 DNA-binding transcriptional dual regulator (NCBI) compare
b2299 yfcD -0.4 -2.1 predicted NUDIX hydrolase (NCBI) compare
b4590 ybfK -0.4 -0.6 hypothetical protein (RefSeq) compare
b2569 lepA -0.4 -1.9 GTP-binding protein LepA (NCBI) compare
b1351 racC -0.4 -0.3 Rac prophage; predicted protein (NCBI) compare
b1630 rsxD -0.4 -2.3 electron transport complex protein RnfD (NCBI) compare
b1690 ydiM -0.4 -1.3 predicted transporter (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms