Experiment set42IT002 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time8hr

200 most detrimental genes:

  gene name fitness t score description  
b1332 ynaJ +1.0 0.9 predicted inner membrane protein (NCBI) compare
b3860 dsbA +1.0 8.6 periplasmic protein disulfide isomerase I (NCBI) compare
b1185 dsbB +1.0 4.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1936 intG +0.9 1.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1831 proQ +0.8 3.2 putative solute/DNA competence effector (NCBI) compare
b1476 fdnI +0.8 1.5 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b3790 rffC +0.8 5.1 TDP-fucosamine acetyltransferase (RefSeq) compare
b4481 rffT +0.8 5.4 4-alpha-L-fucosyltransferase (NCBI) compare
b4538 yoeF +0.7 2.3 hypothetical protein (RefSeq) compare
b2156 lysP +0.7 6.8 lysine transporter (NCBI) compare
b1176 minC +0.6 2.2 septum formation inhibitor (NCBI) compare
b2510 b2510 +0.6 2.6 orf, hypothetical protein (VIMSS) compare
b3787 rffD +0.6 5.5 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3794 rffM +0.6 5.0 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3791 rffA +0.6 4.9 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0798 ybiA +0.6 2.3 hypothetical protein (NCBI) compare
b3786 rffE +0.6 5.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0843 ybjH +0.6 1.8 hypothetical protein (NCBI) compare
b4503 yafF +0.6 0.9 no description compare
b1954 dsrA +0.6 2.8 regulatory antisense RNA (NCBI) compare
b3784 rfe +0.6 5.1 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1195 ymgE +0.6 1.7 predicted inner membrane protein (NCBI) compare
b4410 ecnA +0.5 1.6 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b4480 hdfR +0.5 3.7 transcriptional regulator HdfR (NCBI) compare
b1830 prc +0.5 3.8 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3396 mrcA +0.5 5.2 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b4701 sokX +0.5 0.8 sok-related sRNA, function unknown (RefSeq) compare
b2444 yffM +0.5 2.1 CPZ-55 prophage; predicted protein (NCBI) compare
b1765 ydjA +0.5 0.9 predicted oxidoreductase (NCBI) compare
b1739 osmE +0.5 2.4 DNA-binding transcriptional activator (NCBI) compare
b0630 lipB +0.5 0.9 protein of lipoate biosynthesis (VIMSS) compare
b4551 yheV +0.5 2.4 hypothetical protein (NCBI) compare
b2433 b2433 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b2934 cmtB +0.5 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1382 ynbE +0.5 1.4 predicted lipoprotein (NCBI) compare
b3021 ygiT +0.4 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1183 umuD +0.4 1.5 DNA polymerase V, subunit D (NCBI) compare
b3147 yraM +0.4 3.9 hypothetical protein (NCBI) compare
b0542 renD +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b0183 rnhB +0.4 2.0 ribonuclease HII (NCBI) compare
b1448 yncA +0.4 1.3 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3250 mreC +0.4 1.6 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b2587 kgtP +0.4 2.9 alpha-ketoglutarate transporter (NCBI) compare
b1138 ymfE +0.4 1.1 e14 prophage; predicted inner membrane protein (NCBI) compare
b1758 b1758 +0.4 1.0 putative cytochrome oxidase (VIMSS) compare
b4603 rseX +0.4 0.5 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1251 yciI +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1753 ynjA +0.4 1.5 hypothetical protein (NCBI) compare
b1742 b1742 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b2741 rpoS +0.4 2.5 RNA polymerase sigma factor (NCBI) compare
b4609 ryfD +0.4 2.3 Novel sRNA, function unknown (RefSeq) compare
b1408 b1408 +0.4 2.3 probable enzyme (VIMSS) compare
b1792 yeaO +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b4573 insZ +0.4 2.4 no description compare
b3100 yqjK +0.4 2.2 hypothetical protein (NCBI) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b1434 ydcN +0.4 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2486 hyfF +0.4 2.5 NADH dehydrogenase subunit N (NCBI) compare
b4157 yjeN +0.4 1.1 hypothetical protein (NCBI) compare
b0920 ycbC +0.3 1.6 conserved inner membrane protein (NCBI) compare
b1919 yedO +0.3 1.2 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b0388 aroL +0.3 1.9 shikimate kinase II (NCBI) compare
b4000 hupA +0.3 2.0 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3483 yhhH +0.3 0.2 orf, hypothetical protein (VIMSS) compare
b2051 wcaH +0.3 0.8 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0946 ycbW +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b4529 ydbJ +0.3 0.8 hypothetical protein (NCBI) compare
b4686 yshB +0.3 1.2 expressed protein (RefSeq) compare
b1305 pspB +0.3 1.8 phage shock protein B (NCBI) compare
b0880 cspD +0.3 1.1 cold shock protein homolog (NCBI) compare
b4660 yhiL +0.3 0.2 no description compare
b4533 ynfO +0.3 1.0 Qin prophage; predicted protein (NCBI) compare
b3027 ygiZ +0.3 0.2 conserved inner membrane protein (NCBI) compare
b2014 yeeF +0.3 2.2 putative amino acid/amine transport protein (VIMSS) compare
b3504 yhiS +0.3 0.3 hypothetical protein (RefSeq) compare
b1252 tonB +0.3 1.4 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1348 lar +0.3 1.0 Rac prophage; restriction alleviation protein (NCBI) compare
b1724 ydiZ +0.3 0.9 hypothetical protein (NCBI) compare
b4431 rprA +0.3 1.2 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3961 oxyR +0.3 2.6 DNA-binding transcriptional dual regulator (NCBI) compare
b4642 yoeD +0.3 1.1 no description compare
b1227 narI +0.3 1.7 nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI) compare
b3238 yhcN +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1566 flxA +0.3 1.4 Qin prophage; predicted protein (NCBI) compare
b2209 eco +0.3 0.6 ecotin precursor (NCBI) compare
b1113 ycfS +0.3 2.2 hypothetical protein (NCBI) compare
b4376 osmY +0.3 1.9 periplasmic protein (NCBI) compare
b1421 trg +0.3 1.8 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1469 narU +0.3 1.9 nitrate/nitrite transporter (NCBI) compare
b0942 ycbU +0.3 1.9 predicted fimbrial-like adhesin protein (NCBI) compare
b0707 ybgA +0.3 0.9 hypothetical protein (NCBI) compare
b2208 napF +0.3 1.8 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b0852 rimK +0.3 2.2 ribosomal protein S6 modification protein (NCBI) compare
b1114 mfd +0.3 2.2 transcription-repair coupling factor (NCBI) compare
b2250 b2250 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b3512 GadE +0.3 1.8 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1342 ydaN +0.3 2.0 zinc transporter (NCBI) compare
b3764 yifE +0.3 1.8 hypothetical protein (NCBI) compare
b3672 ivbL +0.3 1.7 ilvB operon leader peptide (NCBI) compare
b1446 ydcY +0.3 1.3 hypothetical protein (NCBI) compare
b2082 ogrK +0.3 1.7 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b0521 ybcF +0.3 2.1 predicted carbamate kinase (NCBI) compare
b2264 menD +0.3 2.4 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b4360 yjjA +0.3 2.0 putative glycoprotein/receptor (VIMSS) compare
b4367 fhuF +0.3 2.1 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b1515 lsrD +0.3 1.5 AI2 transporter (NCBI) compare
b0191 yaeJ +0.3 2.1 hypothetical protein (NCBI) compare
b3623 rfaK +0.3 1.0 lipopolysaccharide core biosynthesis (NCBI) compare
b1238 tdk +0.3 2.0 thymidine kinase (NCBI) compare
b4380 yjjI +0.3 2.4 hypothetical protein (NCBI) compare
b4460 araH +0.3 1.0 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1731 cedA +0.3 1.1 cell division modulator (RefSeq) compare
b3844 fre +0.3 1.7 NAD(P)H-flavin reductase (NCBI) compare
b1179 ycgL +0.3 1.4 conserved protein (NCBI) compare
b0819 ybiS +0.3 2.3 hypothetical protein (NCBI) compare
b3484 yhhI +0.3 1.1 predicted transposase (NCBI) compare
b0803 ybiI +0.3 1.8 hypothetical protein (NCBI) compare
b4505 insX +0.3 0.7 predicted IS protein (RefSeq) compare
b0641 rlpB +0.3 1.2 minor lipoprotein (NCBI) compare
b0851 nfsA +0.3 1.6 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b4546 ypeB +0.3 0.6 hypothetical protein (NCBI) compare
b1669 ydhT +0.3 0.9 hypothetical protein (NCBI) compare
b1618 uidR +0.3 1.8 DNA-binding transcriptional repressor (NCBI) compare
b1733 chbG +0.3 0.9 hypothetical protein (NCBI) compare
b4388 serB +0.3 2.3 3-phosphoserine phosphatase (NCBI) compare
b1349 recT +0.3 1.5 Rac prophage; recombination and repair protein (NCBI) compare
b1656 sodB +0.3 1.0 superoxide dismutase, Fe (NCBI) compare
b4313 fimE +0.3 2.2 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1542 ydfI +0.3 0.9 predicted mannonate dehydrogenase (NCBI) compare
b1764 selD +0.3 1.6 selenophosphate synthetase (NCBI) compare
b1823 cspC +0.3 0.7 stress protein, member of the CspA-family (NCBI) compare
b2782 chpA +0.3 1.7 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b1811 yoaH +0.3 1.8 hypothetical protein (NCBI) compare
b2212 alkB +0.3 1.4 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b1013 ycdC +0.3 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2356 yfdM +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b0766 ybhA +0.3 1.8 predicted hydrolase (NCBI) compare
b1562 hokD +0.3 1.2 Qin prophage; small toxic polypeptide (NCBI) compare
b0726 sucA +0.3 1.4 alpha-ketoglutarate decarboxylase (NCBI) compare
b0226 dinJ +0.3 1.4 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1355 b1355 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1072 flgA +0.3 1.6 flagellar basal body P-ring biosynthesis protein A (NCBI) compare
b2565 recO +0.3 1.6 DNA repair protein RecO (NCBI) compare
b0617 citD +0.3 0.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1097 yceG +0.3 1.8 predicted aminodeoxychorismate lyase (NCBI) compare
b3686 ibpB +0.3 2.1 heat shock chaperone (NCBI) compare
b4513 kdpF +0.3 0.7 potassium ion accessory transporter subunit (NCBI) compare
b2586 yfiM +0.3 1.3 predicted protein (RefSeq) compare
b0102 yacF +0.3 1.4 hypothetical protein (NCBI) compare
b2645 yfjZ +0.3 1.6 CP4-57 prophage; antitoxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b1848 yebG +0.2 0.7 conserved protein regulated by LexA (NCBI) compare
b1698 ydiR +0.2 1.7 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b1070 flgN +0.2 0.7 export chaperone for FlgK and FlgL (NCBI) compare
b1590 ynfH +0.2 1.4 oxidoreductase, membrane subunit (NCBI) compare
b2488 hyfH +0.2 1.4 hydrogenase 4, Fe-S subunit (NCBI) compare
b4596 yciZ +0.2 0.6 hypothetical protein (RefSeq) compare
b3120 yhaB +0.2 0.7 orf, hypothetical protein (VIMSS) compare
b1307 pspD +0.2 0.9 peripheral inner membrane phage-shock protein (NCBI) compare
b1463 nhoA +0.2 2.0 N-hydroxyarylamine O-acetyltransferase (NCBI) compare
b2195 ccmG +0.2 0.9 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1264 trpE +0.2 1.8 anthranilate synthase component I (NCBI) compare
b4241 treR +0.2 2.2 trehalose repressor (NCBI) compare
b1462 b1462 +0.2 0.9 orf, hypothetical protein (VIMSS) compare
b1038 csgF +0.2 1.1 predicted transport protein (NCBI) compare
b4386 lplA +0.2 1.9 lipoate-protein ligase A (NCBI) compare
b3990 thiH +0.2 1.9 thiamine biosynthesis protein ThiH (NCBI) compare
b2221 atoD +0.2 1.3 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b3269 yhdX +0.2 2.0 predicted amino-acid transporter subunit (NCBI) compare
b2263 yfbB +0.2 0.9 predicted peptidase (NCBI) compare
b4385 yjjJ +0.2 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1625 ydgT +0.2 1.5 predicted regulator (NCBI) compare
b3973 birA +0.2 0.9 biotin--protein ligase (NCBI) compare
b0018 mokC +0.2 0.7 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b2535 csiE +0.2 2.1 orf, hypothetical protein (VIMSS) compare
b3236 mdh +0.2 1.8 malate dehydrogenase (NCBI) compare
b1073 flgB +0.2 1.4 flagellar basal-body rod protein B (NCBI) compare
b4381 deoC +0.2 1.6 deoxyribose-phosphate aldolase (NCBI) compare
b0807 ybiN +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b1233 ychJ +0.2 1.6 hypothetical protein (NCBI) compare
b2291 yfbR +0.2 1.5 hypothetical protein (NCBI) compare
b4042 dgkA +0.2 0.1 diacylglycerol kinase (NCBI) compare
b2617 smpA +0.2 1.3 small membrane protein A (VIMSS) compare
b0730 mngR +0.2 0.5 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1530 marR +0.2 1.0 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b2996 hybA +0.2 1.8 hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI) compare
b4358 yjjN +0.2 1.9 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq) compare
b3940 metL +0.2 2.0 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b4017 arpA +0.2 1.3 regulator of acetyl CoA synthetase (NCBI) compare
b2317 dedA +0.2 1.5 conserved inner membrane protein (NCBI) compare
b2165 yeiN +0.2 1.5 hypothetical protein (NCBI) compare
b0193 yaeF +0.2 1.8 orf, hypothetical protein (VIMSS) compare
b4108 phnA +0.2 1.3 hypothetical protein (NCBI) compare
b3698 yidB +0.2 1.3 orf, hypothetical protein (VIMSS) compare
b1789 yeaL +0.2 1.0 conserved inner membrane protein (NCBI) compare
b3752 rbsK +0.2 2.0 ribokinase (NCBI) compare
b1558 cspF +0.2 0.9 Qin prophage; cold shock protein (NCBI) compare
b1033 ycdW +0.2 1.6 putative dehydrogenase (VIMSS) compare
b0933 ssuB +0.2 1.2 alkanesulfonate transporter subunit (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms