Experiment set41IT069 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time6hr

200 most detrimental genes:

  gene name fitness t score description  
b1185 dsbB +1.1 6.4 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b0730 mngR +1.1 2.4 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3860 dsbA +1.0 9.1 periplasmic protein disulfide isomerase I (NCBI) compare
b1831 proQ +0.9 2.7 putative solute/DNA competence effector (NCBI) compare
b0630 lipB +0.8 1.4 protein of lipoate biosynthesis (VIMSS) compare
b2699 recA +0.8 1.2 recombinase A (NCBI) compare
b1954 dsrA +0.8 3.6 regulatory antisense RNA (NCBI) compare
b2934 cmtB +0.7 0.6 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2156 lysP +0.7 6.4 lysine transporter (NCBI) compare
b4505 insX +0.6 1.3 predicted IS protein (RefSeq) compare
b4596 yciZ +0.6 1.6 hypothetical protein (RefSeq) compare
b4551 yheV +0.6 3.1 hypothetical protein (NCBI) compare
b1476 fdnI +0.6 1.0 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b0798 ybiA +0.6 2.6 hypothetical protein (NCBI) compare
b1357 ydaS +0.6 1.8 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4481 rffT +0.6 4.1 4-alpha-L-fucosyltransferase (NCBI) compare
b2444 yffM +0.5 1.4 CPZ-55 prophage; predicted protein (NCBI) compare
b1733 chbG +0.5 1.9 hypothetical protein (NCBI) compare
b4538 yoeF +0.5 1.4 hypothetical protein (RefSeq) compare
b0542 renD +0.5 2.1 orf, hypothetical protein (VIMSS) compare
b1195 ymgE +0.5 2.3 predicted inner membrane protein (NCBI) compare
b3790 rffC +0.5 2.8 TDP-fucosamine acetyltransferase (RefSeq) compare
b3787 rffD +0.5 4.4 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1739 osmE +0.5 2.1 DNA-binding transcriptional activator (NCBI) compare
b1573 ydfC +0.5 0.9 Qin prophage; predicted protein (RefSeq) compare
b3786 rffE +0.5 4.2 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1742 b1742 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b3784 rfe +0.5 4.3 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b3794 rffM +0.5 3.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3163 nlpI +0.5 2.4 hypothetical protein (NCBI) compare
b0933 ssuB +0.4 2.2 alkanesulfonate transporter subunit (NCBI) compare
b4480 hdfR +0.4 3.3 transcriptional regulator HdfR (NCBI) compare
b3791 rffA +0.4 3.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1038 csgF +0.4 1.4 predicted transport protein (NCBI) compare
b3764 yifE +0.4 2.1 hypothetical protein (NCBI) compare
b1753 ynjA +0.4 1.3 hypothetical protein (NCBI) compare
b3396 mrcA +0.4 4.0 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b3021 ygiT +0.4 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b4000 hupA +0.4 2.2 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1143 ymfI +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b0843 ybjH +0.4 1.0 hypothetical protein (NCBI) compare
b3624 rfaZ +0.4 1.4 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1765 ydjA +0.4 0.6 predicted oxidoreductase (NCBI) compare
b3147 yraM +0.4 3.4 hypothetical protein (NCBI) compare
b2741 rpoS +0.4 2.6 RNA polymerase sigma factor (NCBI) compare
b2432 yfeY +0.4 1.4 hypothetical protein (NCBI) compare
b2586 yfiM +0.4 1.7 predicted protein (RefSeq) compare
b4520 ymgF +0.4 0.9 hypothetical protein (NCBI) compare
b1961 dcm +0.4 0.9 DNA cytosine methylase (NCBI) compare
b4704 arrS +0.4 0.2 Antisense sRNA ArrS, function unknown (NCBI) compare
b3206 npr +0.4 2.6 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b3181 greA +0.4 2.2 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b2915 yqfE +0.4 1.2 hypothetical protein (RefSeq) compare
b0819 ybiS +0.4 2.9 hypothetical protein (NCBI) compare
b4509 ylcG +0.4 0.9 DLP12 prophage; predicted protein (NCBI) compare
b3483 yhhH +0.4 0.2 orf, hypothetical protein (VIMSS) compare
b3027 ygiZ +0.4 0.2 conserved inner membrane protein (NCBI) compare
b4376 osmY +0.4 1.7 periplasmic protein (NCBI) compare
b3259 prmA +0.3 2.5 ribosomal protein L11 methyltransferase (NCBI) compare
b1238 tdk +0.3 2.1 thymidine kinase (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b4614 sokA +0.3 1.5 no description compare
b3863 polA +0.3 0.5 DNA polymerase I (NCBI) compare
b4573 insZ +0.3 1.5 no description compare
b1847 yebF +0.3 0.7 predicted protein (RefSeq) compare
b1948 fliP +0.3 1.8 flagellar biosynthesis protein P (NCBI) compare
b2212 alkB +0.3 1.7 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b2010 dacD +0.3 2.4 penicillin binding protein 6b (VIMSS) compare
b1590 ynfH +0.3 1.8 oxidoreductase, membrane subunit (NCBI) compare
b2014 yeeF +0.3 2.3 putative amino acid/amine transport protein (VIMSS) compare
b1134 ymfB +0.3 1.5 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b4515 ybgT +0.3 1.1 hypothetical protein (NCBI) compare
b4613 dinQ +0.3 1.2 Damage inducible, function unknown (RefSeq) compare
b3961 oxyR +0.3 2.8 DNA-binding transcriptional dual regulator (NCBI) compare
b2587 kgtP +0.3 2.1 alpha-ketoglutarate transporter (NCBI) compare
b1651 gloA +0.3 1.0 glyoxalase I, Ni-dependent (NCBI) compare
b1058 yceO +0.3 0.2 hypothetical protein (NCBI) compare
b3261 fis +0.3 0.5 DNA-binding protein Fis (NCBI) compare
b1515 lsrD +0.3 1.6 AI2 transporter (NCBI) compare
b2782 chpA +0.3 2.0 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b1138 ymfE +0.3 0.9 e14 prophage; predicted inner membrane protein (NCBI) compare
b1758 b1758 +0.3 0.8 putative cytochrome oxidase (VIMSS) compare
b1558 cspF +0.3 1.3 Qin prophage; cold shock protein (NCBI) compare
b0713 ybgL +0.3 2.1 hypothetical protein (NCBI) compare
b2202 napC +0.3 2.0 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1035 ycdY +0.3 2.1 hypothetical protein (NCBI) compare
b0852 rimK +0.3 2.2 ribosomal protein S6 modification protein (NCBI) compare
b1348 lar +0.3 0.9 Rac prophage; restriction alleviation protein (NCBI) compare
b4313 fimE +0.3 2.4 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4394 yjjX +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1063 yceB +0.3 1.7 predicted lipoprotein (NCBI) compare
b1418 cybB +0.3 1.8 cytochrome b(561) (VIMSS) compare
b1789 yeaL +0.3 1.0 conserved inner membrane protein (NCBI) compare
b4277 yjgZ +0.3 2.3 KpLE2 phage-like element; predicted protein (NCBI) compare
b4363 yjjB +0.3 1.5 conserved inner membrane protein (RefSeq) compare
b2209 eco +0.3 1.0 ecotin precursor (NCBI) compare
b0617 citD +0.3 1.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1724 ydiZ +0.3 1.0 hypothetical protein (NCBI) compare
b4646 yrdE +0.3 1.0 no description compare
b1478 adhP +0.3 1.5 alcohol dehydrogenase (VIMSS) compare
b2792 yqcC +0.3 1.2 hypothetical protein (NCBI) compare
b1919 yedO +0.3 0.9 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0696 kdpC +0.3 1.6 potassium-transporting ATPase subunit C (NCBI) compare
b4158 yjeO +0.3 1.9 conserved inner membrane protein (NCBI) compare
b1096 pabC +0.3 2.0 4-amino-4-deoxychorismate lyase (NCBI) compare
b0183 rnhB +0.3 1.7 ribonuclease HII (NCBI) compare
b2565 recO +0.3 1.6 DNA repair protein RecO (NCBI) compare
b0381 ddlA +0.3 1.9 D-alanylalanine synthetase (NCBI) compare
b3686 ibpB +0.3 2.1 heat shock chaperone (NCBI) compare
b4656 yjhY +0.3 1.5 no description compare
b2004 yeeU +0.3 1.5 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b4642 yoeD +0.3 1.0 no description compare
b1566 flxA +0.3 1.1 Qin prophage; predicted protein (NCBI) compare
b4609 ryfD +0.3 1.4 Novel sRNA, function unknown (RefSeq) compare
b1184 umuC +0.3 2.2 DNA polymerase V subunit UmuC (NCBI) compare
b1085 b1085 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2535 csiE +0.3 2.4 orf, hypothetical protein (VIMSS) compare
b3250 mreC +0.3 0.8 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b2252 ais +0.3 1.6 hypothetical protein (NCBI) compare
b3236 mdh +0.3 2.2 malate dehydrogenase (NCBI) compare
b4331 yjiI +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b2486 hyfF +0.3 1.9 NADH dehydrogenase subunit N (NCBI) compare
b2250 b2250 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0388 aroL +0.3 1.7 shikimate kinase II (NCBI) compare
b2897 ygfY +0.3 1.6 hypothetical protein (NCBI) compare
b2488 hyfH +0.3 1.2 hydrogenase 4, Fe-S subunit (NCBI) compare
b4698 mgrR +0.3 1.2 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b2736 ygbJ +0.3 1.4 predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b2510 b2510 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b0210 yafE +0.3 1.3 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b2476 purC +0.3 0.8 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b3627 rfaI +0.3 2.2 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1276 acnA +0.3 1.8 aconitate hydratase (NCBI) compare
b3836 b3836 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b0848 ybjM +0.3 1.6 predicted inner membrane protein (NCBI) compare
b3156 yhbS +0.3 2.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b3152 yraR +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b0461 ybaJ +0.3 1.8 hypothetical protein (NCBI) compare
b2738 ygbL +0.3 1.7 hypothetical protein (NCBI) compare
b4441 glmY +0.3 0.8 sRNA activator of glmS mRNA (RefSeq) compare
b2264 menD +0.3 1.8 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b4582 yoeA +0.2 1.4 no description compare
b3476 nikA +0.2 2.2 nickel transporter subunit (NCBI) compare
b3940 metL +0.2 2.1 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b2071 yegJ +0.2 0.9 hypothetical protein (NCBI) compare
b4045 yjbJ +0.2 1.3 predicted stress response protein (NCBI) compare
b1427 rimL +0.2 1.0 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b2452 eutH +0.2 1.7 predicted inner membrane protein (NCBI) compare
b3393 yrfB +0.2 1.8 hypothetical protein (NCBI) compare
b1511 ydeV +0.2 1.3 putative kinase (VIMSS) compare
b2804 fucU +0.2 1.1 L-fucose mutarotase (NCBI) compare
b1776 ydjL +0.2 1.5 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI) compare
b1930 yedF +0.2 1.4 hypothetical protein (NCBI) compare
b1837 yebW +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b1419 ydcA +0.2 1.4 hypothetical protein (NCBI) compare
b1361 ydaW +0.2 0.2 orf, hypothetical protein (VIMSS) compare
b1305 pspB +0.2 1.1 phage shock protein B (NCBI) compare
b2261 menC +0.2 1.7 O-succinylbenzoate synthase (NCBI) compare
b1963 b1963 +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b4450 arcZ +0.2 1.5 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1725 yniA +0.2 1.5 predicted phosphotransferase/kinase (NCBI) compare
b0393 rdgC +0.2 2.0 recombination associated protein (NCBI) compare
b2968 yghD +0.2 1.6 predicted secretion pathway M-type protein, membrane anchored (NCBI) compare
b1572 ydfB +0.2 0.8 orf, hypothetical protein (VIMSS) compare
b1349 recT +0.2 1.6 Rac prophage; recombination and repair protein (NCBI) compare
b2471 yffB +0.2 1.1 hypothetical protein (NCBI) compare
b1907 tyrP +0.2 1.2 tyrosine transporter (NCBI) compare
b3032 cpdA +0.2 1.7 cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI) compare
b0545 ybcL +0.2 1.9 DLP12 prophage; predicted kinase inhibitor (NCBI) compare
b3406 greB +0.2 1.6 transcription elongation factor GreB (NCBI) compare
b4128 yjdK +0.2 0.2 hypothetical protein (NCBI) compare
b0407 yajC +0.2 0.5 preprotein translocase subunit YajC (NCBI) compare
b4099 phnI +0.2 1.6 carbon-phosphorus lyase complex subunit (NCBI) compare
b2665 ygaU +0.2 1.6 hypothetical protein (NCBI) compare
b4367 fhuF +0.2 1.9 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b4017 arpA +0.2 1.2 regulator of acetyl CoA synthetase (NCBI) compare
b4365 yjjQ +0.2 2.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2165 yeiN +0.2 1.5 hypothetical protein (NCBI) compare
b2700 ygaD +0.2 0.9 competence damage-inducible protein A (NCBI) compare
b0682 ybfN +0.2 1.5 predicted lipoprotein (NCBI) compare
b1068 mviM +0.2 1.7 predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b3446 yrhB +0.2 1.5 hypothetical protein (NCBI) compare
b0864 artP +0.2 1.0 arginine transporter subunit (NCBI) compare
b2644 yfjY +0.2 1.1 CP4-57 prophage; predicted DNA repair protein (NCBI) compare
b0521 ybcF +0.2 1.1 predicted carbamate kinase (NCBI) compare
b3623 rfaK +0.2 0.7 lipopolysaccharide core biosynthesis (NCBI) compare
b3260 dusB +0.2 1.6 tRNA-dihydrouridine synthase B (NCBI) compare
b0765 modC +0.2 1.4 molybdate transporter subunit (NCBI) compare
b1150 ymfR +0.2 0.5 e14 prophage; predicted protein (NCBI) compare
b4675 yoaJ +0.2 0.7 expressed protein (RefSeq) compare
b2213 ada +0.2 1.2 fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI) compare
b0340 cynS +0.2 1.3 cyanate hydratase (NCBI) compare
b1074 flgC +0.2 1.0 flagellar basal-body rod protein C (NCBI) compare
b2445 yffN +0.2 0.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b4444 rygA +0.2 1.1 unknown RNA (NCBI) compare
b1967 hchA +0.2 1.7 chaperone protein HchA (NCBI) compare
b0859 rumB +0.2 1.8 23S rRNA m(5)U747-methyltransferase (NCBI) compare
b1287 yciW +0.2 0.9 putative oxidoreductase (VIMSS) compare
b1792 yeaO +0.2 1.0 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms