Experiment set41IT067 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time5hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -7.5 -12.5 alanine racemase (NCBI) compare
b0462 acrB -7.1 -30.3 multidrug efflux system protein (NCBI) compare
b0463 acrA -6.9 -21.3 multidrug efflux system (NCBI) compare
b4237 nrdG -5.6 -6.6 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.2 -8.7 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.1 -5.1 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4201 priB -3.6 -6.0 primosomal replication protein N (NCBI) compare
b1334 fnr -2.7 -2.5 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b0386 proC -2.3 -6.0 pyrroline-5-carboxylate reductase (NCBI) compare
b3035 tolC -2.2 -1.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b1850 eda -2.0 -2.6 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b4202 rpsR -1.9 -2.8 30S ribosomal protein S18 (NCBI) compare
b0243 proA -1.8 -10.8 gamma-glutamyl phosphate reductase (NCBI) compare
b4025 pgi -1.8 -11.2 glucose-6-phosphate isomerase (NCBI) compare
b2620 smpB -1.7 -1.8 SsrA-binding protein (NCBI) compare
b1192 ldcA -1.7 -2.6 L,D-carboxypeptidase A (NCBI) compare
b2912 ygfA -1.7 -3.7 putative ligase (VIMSS) compare
b0902 pflA -1.7 -8.4 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b1187 fadR -1.6 -3.3 fatty acid metabolism regulator (NCBI) compare
b0200 gmhB -1.6 -1.8 hypothetical protein (NCBI) compare
b0949 uup -1.5 -10.0 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0912 ihfB -1.4 -2.7 integration host factor subunit beta (NCBI) compare
b0242 proB -1.4 -6.1 gamma-glutamyl kinase (NCBI) compare
b1804 rnd -1.4 -6.8 ribonuclease D (NCBI) compare
b1860 ruvB -1.3 -1.4 Holliday junction DNA helicase B (NCBI) compare
b2820 recB -1.2 -0.8 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1290 sapF -1.2 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1855 lpxM -1.2 -4.6 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1236 galU -1.1 -4.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3868 glnG -1.1 -6.4 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b3500 gor -1.1 -2.6 glutathione reductase (NCBI) compare
b4593 ymgI -1.1 -1.3 hypothetical protein (RefSeq) compare
b3390 aroK -1.1 -2.6 shikimate kinase I (NCBI) compare
b3781 trxA -1.1 -4.9 thioredoxin 1 (VIMSS) compare
b3741 gidA -1.1 -2.9 glucose-inhibited division protein A (NCBI) compare
b0149 mrcB -1.0 -8.7 penicillin-binding protein 1b (NCBI) compare
b0741 pal -1.0 -3.2 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0688 pgm -1.0 -3.2 phosphoglucomutase (NCBI) compare
b1863 ruvC -1.0 -1.5 Holliday junction resolvase (NCBI) compare
b0303 ykgI -1.0 -0.9 orf, hypothetical protein (VIMSS) compare
b2659 b2659 -1.0 -3.3 orf, hypothetical protein (VIMSS) compare
b0728 sucC -0.9 -5.8 succinyl-CoA synthetase subunit beta (NCBI) compare
b4066 yjcF -0.9 -0.6 hypothetical protein (NCBI) compare
b2576 srmB -0.9 -3.2 ATP-dependent RNA helicase (NCBI) compare
b2501 ppk -0.9 -6.3 polyphosphate kinase (NCBI) compare
b0903 pflB -0.9 -6.4 pyruvate formate lyase I (NCBI) compare
b2528 iscA -0.9 -1.4 iron-sulfur cluster assembly protein (NCBI) compare
b0167 glnD -0.9 -3.8 PII uridylyl-transferase (NCBI) compare
b1123 potD -0.9 -5.4 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b2814 metZ -0.9 -2.0 tRNA-Met (NCBI) compare
b0739 tolA -0.8 -2.1 cell envelope integrity inner membrane protein TolA (NCBI) compare
b4701 sokX -0.8 -1.0 sok-related sRNA, function unknown (RefSeq) compare
b2699 recA -0.8 -0.5 recombinase A (NCBI) compare
b0908 aroA -0.8 -5.6 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3493 pitA -0.8 -5.5 phosphate transporter, low-affinity (NCBI) compare
b2704 srlB -0.8 -4.3 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b4255 yjgD -0.8 -4.8 hypothetical protein (NCBI) compare
b3930 menA -0.8 -7.0 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b2329 aroC -0.8 -4.2 chorismate synthase (NCBI) compare
b1712 ihfA -0.8 -0.5 integration host factor subunit alpha (NCBI) compare
b1124 potC -0.8 -4.5 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3935 priA -0.8 -2.8 primosome assembly protein PriA (NCBI) compare
b4451 ryhB -0.8 -2.6 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2110 yehC -0.8 -1.4 predicted periplasmic pilin chaperone (NCBI) compare
b3842 rfaH -0.8 -4.1 transcriptional activator RfaH (NCBI) compare
b3728 pstS -0.8 -3.2 phosphate transporter subunit (NCBI) compare
b1104 ycfL -0.7 -2.5 hypothetical protein (NCBI) compare
b0014 dnaK -0.7 -2.7 molecular chaperone DnaK (NCBI) compare
b2318 truA -0.7 -2.5 tRNA pseudouridine synthase A (NCBI) compare
b1253 yciA -0.7 -2.8 predicted hydrolase (NCBI) compare
b1304 pspA -0.7 -3.4 regulatory protein for phage-shock-protein operon (NCBI) compare
b3162 deaD -0.7 -3.5 inducible ATP-independent RNA helicase (VIMSS) compare
b1259 yciG -0.7 -1.9 orf, hypothetical protein (VIMSS) compare
b4545 ypdJ -0.7 -2.5 no description compare
b1628 rsxB -0.7 -3.1 electron transport complex protein RnfB (NCBI) compare
b0222 lpcA -0.7 -2.0 phosphoheptose isomerase (NCBI) compare
b2531 iscR -0.7 -1.4 DNA-binding transcriptional repressor (NCBI) compare
b0740 tolB -0.7 -1.8 translocation protein TolB precursor (NCBI) compare
b2822 recC -0.7 -3.8 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b3183 obgE -0.7 -1.0 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1589 ynfG -0.7 -1.7 oxidoreductase, Fe-S subunit (NCBI) compare
b0737 tolQ -0.7 -1.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4402 yjjY -0.7 -0.8 hypothetical protein (NCBI) compare
b3706 trmE -0.7 -2.6 tRNA modification GTPase (NCBI) compare
b1126 potA -0.6 -3.7 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b3619 rfaD -0.6 -2.4 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1293 sapB -0.6 -1.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2297 pta -0.6 -0.5 phosphate acetyltransferase (NCBI) compare
b2625 yfjI -0.6 -1.3 CP4-57 prophage; predicted protein (NCBI) compare
b0729 sucD -0.6 -3.3 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3977 tyrU -0.6 -0.8 tRNA-Tyr (NCBI) compare
b0560 nohB -0.6 -2.9 DLP12 prophage; DNA packaging protein (NCBI) compare
b1217 chaB -0.6 -1.7 cation transport regulator (NCBI) compare
b3806 cyaA -0.6 -3.9 adenylate cyclase (NCBI) compare
b4375 prfC -0.6 -1.8 peptide chain release factor 3 (NCBI) compare
b1271 yciK -0.6 -2.2 short chain dehydrogenase (NCBI) compare
b2830 nudH -0.6 -0.9 dinucleoside polyphosphate hydrolase (NCBI) compare
b2352 yfdI -0.6 -0.6 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b1292 sapC -0.6 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2553 glnB -0.6 -2.5 regulatory protein P-II for glutamine synthetase (NCBI) compare
b3119 tdcR -0.6 -2.0 threonine dehydratase operon activator protein (VIMSS) compare
b2498 upp -0.6 -1.6 uracil phosphoribosyltransferase (VIMSS) compare
b1125 potB -0.6 -2.0 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2330 yfcB -0.6 -0.8 putative adenine-specific methylase (VIMSS) compare
b1429 tehA -0.6 -1.1 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1286 rnb -0.6 -1.3 exoribonuclease II (NCBI) compare
b1291 sapD -0.6 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1518 lsrG -0.6 -1.1 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1347 ydaC -0.5 -0.3 Rac prophage; predicted protein (NCBI) compare
b3115 tdcD -0.5 -5.1 propionate kinase/acetate kinase C, anaerobic (RefSeq) compare
b4191 ulaR -0.5 -2.4 DNA-binding transcriptional dual regulator (NCBI) compare
b1268 yciQ -0.5 -3.8 predicted inner membrane protein (NCBI) compare
b2024 hisA -0.5 -2.0 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b0970 yccA -0.5 -2.8 inner membrane protein (NCBI) compare
b0705 ybfL -0.5 -0.3 putative receptor protein (VIMSS) compare
b3792 wzxE -0.5 -3.7 O-antigen translocase (NCBI) compare
b0975 hyaD -0.5 -2.9 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b3341 rpsG -0.5 -1.1 30S ribosomal protein S7 (NCBI) compare
b0053 surA -0.5 -3.4 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3281 aroE -0.5 -4.7 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b1595 ynfL -0.5 -2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1527 yneK -0.5 -1.6 hypothetical protein (NCBI) compare
b1540 ydfH -0.5 -1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1505 ydeT -0.5 -0.8 hypothetical protein (RefSeq) compare
b0237 pepD -0.5 -3.6 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b2652 ileY -0.5 -0.6 tRNA-Ile (NCBI) compare
b3052 rfaE -0.5 -2.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b3229 sspA -0.5 -1.3 stringent starvation protein A (NCBI) compare
b2285 nuoE -0.5 -1.6 NADH dehydrogenase subunit E (NCBI) compare
b1171 b1171 -0.5 -1.5 orf, hypothetical protein (VIMSS) compare
b0999 cbpM -0.5 -2.4 modulator of CbpA co-chaperone (NCBI) compare
b2846 yqeH -0.5 -0.4 orf, hypothetical protein (VIMSS) compare
b2951 yggS -0.5 -2.3 predicted enzyme (NCBI) compare
b2104 thiM -0.5 -2.1 hydroxyethylthiazole kinase (NCBI) compare
b3389 aroB -0.5 -4.1 3-dehydroquinate synthase (NCBI) compare
b2614 grpE -0.5 -1.8 heat shock protein (NCBI) compare
b2081 yegQ -0.5 -3.6 predicted peptidase (NCBI) compare
b2479 gcvR -0.5 -2.3 transcriptional regulation of gcv operon (VIMSS) compare
b0357 yaiN -0.5 -1.5 putative alpha helix chain (VIMSS) compare
b2248 yfaX -0.5 -1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1048 mdoG -0.5 -2.8 glucan biosynthesis protein, periplasmic (NCBI) compare
b3026 qseC -0.5 -1.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0500 ybbD -0.5 -0.2 hypothetical protein (RefSeq) compare
b1273 yciN -0.5 -1.3 hypothetical protein (NCBI) compare
b0234 yafP -0.5 -1.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4462 ygaQ -0.5 -1.1 hypothetical protein (RefSeq) compare
b1921 fliZ -0.5 -1.7 orf, hypothetical protein (VIMSS) compare
b0118 acnB -0.5 -0.5 aconitate hydratase (NCBI) compare
b0049 apaH -0.5 -1.1 diadenosinetetraphosphatase (NCBI) compare
b1231 tyrT -0.5 -0.9 tRNA-Tyr (NCBI) compare
b4407 thiS -0.5 -0.8 sulfur carrier protein ThiS (NCBI) compare
b1824 yobF -0.5 -1.6 hypothetical protein (NCBI) compare
b1147 ymfL -0.5 -1.5 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b2562 yfhL -0.5 -3.7 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b0706 ybfD -0.5 -1.7 hypothetical protein (NCBI) compare
b1235 rssB -0.5 -3.0 response regulator of RpoS (NCBI) compare
b3766 ilvL -0.5 -1.9 ilvG operon leader peptide (NCBI) compare
b3387 dam -0.5 -3.6 DNA adenine methylase (NCBI) compare
b0677 nagA -0.4 -2.5 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b4547 ypfN -0.4 -2.5 hypothetical protein (NCBI) compare
b0232 yafN -0.4 -0.6 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4414 tff -0.4 -1.5 unknown RNA (NCBI) compare
b2343 b2343 -0.4 -1.6 orf, hypothetical protein (VIMSS) compare
b1279 yciS -0.4 -0.9 conserved inner membrane protein (NCBI) compare
b3620 rfaF -0.4 -2.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3609 secB -0.4 -2.1 export protein SecB (NCBI) compare
b0738 tolR -0.4 -0.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1222 narX -0.4 -2.7 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b2105 yohL -0.4 -2.1 hypothetical protein (NCBI) compare
b2047 wcaJ -0.4 -2.5 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b1630 rsxD -0.4 -2.4 electron transport complex protein RnfD (NCBI) compare
b4647 mokA -0.4 -0.7 no description compare
b1684 sufA -0.4 -1.9 iron-sulfur cluster assembly scaffold protein (NCBI) compare
b1814 sdaA -0.4 -2.5 L-serine deaminase I (NCBI) compare
b1012 b1012 -0.4 -1.8 orf, hypothetical protein (VIMSS) compare
b2082 ogrK -0.4 -2.1 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b1282 yciH -0.4 -2.1 orf, hypothetical protein (VIMSS) compare
b1536 ydeI -0.4 -0.5 hypothetical protein (NCBI) compare
b1594 dgsA -0.4 -1.8 DNA-binding transcriptional repressor (NCBI) compare
b2012 yeeD -0.4 -1.9 hypothetical protein (NCBI) compare
b1648 b1648 -0.4 -1.2 orf, hypothetical protein (VIMSS) compare
b0082 mraW -0.4 -1.4 S-adenosyl-methyltransferase (NCBI) compare
b4577 sgrS -0.4 -1.5 small antisense RNA (NCBI) compare
b1627 rsxA -0.4 -2.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1634 tppB -0.4 -2.7 putative tripeptide transporter permease (NCBI) compare
b1241 adhE -0.4 -3.2 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b3884 yihW -0.4 -2.9 putative DEOR-type transcriptional regulator (VIMSS) compare
b1861 ruvA -0.4 -0.5 Holliday junction DNA helicase motor protein (NCBI) compare
b1368 ynaA -0.4 -1.7 Rac prophage; predicted tail protein (RefSeq) compare
b1563 relE -0.4 -1.2 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b1617 uidA -0.4 -2.6 beta-D-glucuronidase (NCBI) compare
b2058 wcaB -0.4 -0.8 predicted acyl transferase (NCBI) compare
b1351 racC -0.4 -0.2 Rac prophage; predicted protein (NCBI) compare
b3631 rfaG -0.4 -3.6 glucosyltransferase I (NCBI) compare
b0603 ybdO -0.4 -0.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2266 elaB -0.4 -1.9 hypothetical protein (NCBI) compare
b4179 vacB -0.4 -2.6 putative enzyme (VIMSS) compare
b4552 yrhC -0.4 -0.7 no description compare
b4178 yjeB -0.4 -2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2567 rnc -0.4 -0.2 ribonuclease III (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms