Experiment set41IT067 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time5hr

200 most detrimental genes:

  gene name fitness t score description  
b1361 ydaW +1.2 1.1 orf, hypothetical protein (VIMSS) compare
b1205 ychH +1.1 2.4 predicted inner membrane protein (NCBI) compare
b1185 dsbB +1.1 5.5 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4603 rseX +1.0 1.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3860 dsbA +1.0 8.4 periplasmic protein disulfide isomerase I (NCBI) compare
b1332 ynaJ +0.9 0.7 predicted inner membrane protein (NCBI) compare
b4538 yoeF +0.8 2.1 hypothetical protein (RefSeq) compare
b3120 yhaB +0.7 2.2 orf, hypothetical protein (VIMSS) compare
b4596 yciZ +0.7 1.0 hypothetical protein (RefSeq) compare
b4676 yoaK +0.7 0.7 expressed protein (RefSeq) compare
b1348 lar +0.7 2.4 Rac prophage; restriction alleviation protein (NCBI) compare
b1160 elbA +0.7 1.1 hypothetical protein (NCBI) compare
b1025 ycdT +0.6 1.7 predicted diguanylate cyclase (NCBI) compare
b1408 b1408 +0.6 4.1 probable enzyme (VIMSS) compare
b2356 yfdM +0.6 2.6 orf, hypothetical protein (VIMSS) compare
b2051 wcaH +0.6 1.3 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0542 renD +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b2809 b2809 +0.5 2.5 orf, hypothetical protein (VIMSS) compare
b1357 ydaS +0.5 2.2 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1610 tus +0.5 2.9 DNA replication terminus site-binding protein (NCBI) compare
b0843 ybjH +0.5 1.6 hypothetical protein (NCBI) compare
b0388 aroL +0.5 3.5 shikimate kinase II (NCBI) compare
b1415 aldA +0.5 3.5 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b1399 paaX +0.5 3.0 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b1831 proQ +0.5 2.3 putative solute/DNA competence effector (NCBI) compare
b2504 yfgG +0.5 0.9 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1085 b1085 +0.5 1.7 orf, hypothetical protein (VIMSS) compare
b1753 ynjA +0.5 1.6 hypothetical protein (NCBI) compare
b1724 ydiZ +0.5 1.2 hypothetical protein (NCBI) compare
b1739 osmE +0.5 2.1 DNA-binding transcriptional activator (NCBI) compare
b4480 hdfR +0.5 3.2 transcriptional regulator HdfR (NCBI) compare
b4519 icdC +0.5 2.4 no description compare
b4651 yibW +0.5 0.4 no description compare
b0617 citD +0.5 1.6 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1930 yedF +0.4 3.3 hypothetical protein (NCBI) compare
b4520 ymgF +0.4 1.2 hypothetical protein (NCBI) compare
b1709 btuD +0.4 0.9 vitamin B12-transporter ATPase (NCBI) compare
b1387 maoC +0.4 2.6 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b4506 ykgO +0.4 2.3 rpmJ (L36) paralog (NCBI) compare
b0946 ycbW +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1961 dcm +0.4 1.1 DNA cytosine methylase (NCBI) compare
b4152 frdC +0.4 1.5 fumarate reductase subunit C (NCBI) compare
b1353 sieB +0.4 2.5 phage superinfection exclusion protein (VIMSS) compare
b1936 intG +0.4 0.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1613 manA +0.4 1.4 mannose-6-phosphate isomerase (VIMSS) compare
b2510 b2510 +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b1103 ycfF +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b2071 yegJ +0.4 1.3 hypothetical protein (NCBI) compare
b4529 ydbJ +0.4 0.9 hypothetical protein (NCBI) compare
b1506 yneL +0.4 0.8 predicted transcriptional regulator (NCBI) compare
b1410 ynbC +0.4 2.5 predicted hydrolase (NCBI) compare
b1195 ymgE +0.4 1.8 predicted inner membrane protein (NCBI) compare
b3563 yiaB +0.4 2.6 orf, hypothetical protein (VIMSS) compare
b4410 ecnA +0.4 1.1 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b4546 ypeB +0.4 0.9 hypothetical protein (NCBI) compare
b0798 ybiA +0.4 1.0 hypothetical protein (NCBI) compare
b1414 ydcF +0.4 2.6 hypothetical protein (NCBI) compare
b1431 ydcL +0.4 2.4 predicted lipoprotein (NCBI) compare
b2684 mprA +0.4 1.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0218 yafU +0.4 1.4 predicted inner membrane protein (RefSeq) compare
b2481 hyfA +0.4 2.1 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1419 ydcA +0.4 2.4 hypothetical protein (NCBI) compare
b3764 yifE +0.4 2.6 hypothetical protein (NCBI) compare
b4492 ydbA +0.4 2.8 no description compare
b1422 ydcI +0.4 1.9 putative transcriptional regulator LYSR-type (VIMSS) compare
b1342 ydaN +0.4 2.3 zinc transporter (NCBI) compare
b1461 ydcE +0.4 1.7 4-oxalocrotonate tautomerase (NCBI) compare
b1792 yeaO +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1948 fliP +0.4 2.0 flagellar biosynthesis protein P (NCBI) compare
b1129 phoQ +0.4 0.9 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b3618 htrL +0.3 2.9 involved in lipopolysaccharide biosynthesis (VIMSS) compare
b3021 ygiT +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b2163 yeiL +0.3 2.3 putative transcriptional regulator (VIMSS) compare
b1733 chbG +0.3 1.6 hypothetical protein (NCBI) compare
b1427 rimL +0.3 1.2 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1607 ydgC +0.3 1.1 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1153 ymfQ +0.3 1.1 e14 prophage; conserved protein (NCBI) compare
b1138 ymfE +0.3 0.9 e14 prophage; predicted inner membrane protein (NCBI) compare
b4363 yjjB +0.3 1.7 conserved inner membrane protein (RefSeq) compare
b4376 osmY +0.3 2.0 periplasmic protein (NCBI) compare
b4551 yheV +0.3 1.5 hypothetical protein (NCBI) compare
b2617 smpA +0.3 1.5 small membrane protein A (VIMSS) compare
b1768 ydjB +0.3 2.3 orf, hypothetical protein (VIMSS) compare
b3037 ygiB +0.3 2.0 conserved outer membrane protein (RefSeq) compare
b1434 ydcN +0.3 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2209 eco +0.3 0.9 ecotin precursor (NCBI) compare
b2017 yefM +0.3 0.5 orf, hypothetical protein (VIMSS) compare
b2859 ygeO +0.3 0.8 hypothetical protein (RefSeq) compare
b1070 flgN +0.3 0.9 export chaperone for FlgK and FlgL (NCBI) compare
b2156 lysP +0.3 2.7 lysine transporter (NCBI) compare
b3624 rfaZ +0.3 1.2 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4640 yoeG +0.3 1.1 no description compare
b2804 fucU +0.3 1.6 L-fucose mutarotase (NCBI) compare
b3613 yibP +0.3 1.0 putative membrane protein (VIMSS) compare
b4527 b1354 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b0191 yaeJ +0.3 2.3 hypothetical protein (NCBI) compare
b1424 mdoD +0.3 2.2 glucan biosynthesis protein, periplasmic (RefSeq) compare
b0933 ssuB +0.3 1.7 alkanesulfonate transporter subunit (NCBI) compare
b1469 narU +0.3 2.3 nitrate/nitrite transporter (NCBI) compare
b1689 b1689 +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b4418 sraB +0.3 0.7 unknown RNA (NCBI) compare
b1395 paaH +0.3 2.6 3-hydroxybutyryl-CoA dehydrogenase (NCBI) compare
b1742 b1742 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b0889 lrp +0.3 1.3 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b0183 rnhB +0.3 2.0 ribonuclease HII (NCBI) compare
b1573 ydfC +0.3 0.4 Qin prophage; predicted protein (RefSeq) compare
b0937 ssuE +0.3 1.4 NAD(P)H-dependent FMN reductase (NCBI) compare
b1442 ydcU +0.3 2.2 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1698 ydiR +0.3 1.5 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b2959 yggL +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b4458 oxyS +0.3 0.5 global regulatory RNA (NCBI) compare
b1951 rcsA +0.3 0.7 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b1520 b1520 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b4509 ylcG +0.3 1.0 DLP12 prophage; predicted protein (NCBI) compare
b2741 rpoS +0.3 2.0 RNA polymerase sigma factor (NCBI) compare
b2096 gatY +0.3 1.8 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b3443 yrhA +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b0547 ybcN +0.3 2.0 DLP12 prophage; predicted protein (NCBI) compare
b1463 nhoA +0.3 2.2 N-hydroxyarylamine O-acetyltransferase (NCBI) compare
b0880 cspD +0.3 0.9 cold shock protein homolog (NCBI) compare
b4598 yncL +0.3 1.2 hypothetical protein (RefSeq) compare
b1858 znuC +0.3 2.2 high-affinity zinc transporter ATPase (NCBI) compare
b1394 paaG +0.3 1.7 enoyl-CoA hydratase (NCBI) compare
b3723 bglG +0.3 1.9 transcriptional antiterminator of the bgl operon (NCBI) compare
b2312 purF +0.3 2.3 amidophosphoribosyltransferase (NCBI) compare
b3836 b3836 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b1063 yceB +0.3 1.5 predicted lipoprotein (NCBI) compare
b4646 yrdE +0.3 1.3 no description compare
b1454 yncG +0.3 1.4 predicted enzyme (NCBI) compare
b1467 narY +0.3 1.6 nitrate reductase 2 (NRZ), beta subunit (NCBI) compare
b0589 fepG +0.3 1.9 iron-enterobactin transporter subunit (NCBI) compare
b1107 nagZ +0.3 1.7 beta-hexosaminidase (NCBI) compare
b0535 fimZ +0.3 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1110 ycfJ +0.3 2.0 hypothetical protein (NCBI) compare
b1172 ymgG +0.3 2.0 conserved protein, UPF0757 family (RefSeq) compare
b1390 paaC +0.3 1.9 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b4642 yoeD +0.3 0.9 no description compare
b4579 yaiX +0.3 1.9 no description compare
b4444 rygA +0.3 1.4 unknown RNA (NCBI) compare
b4568 ytjA +0.3 1.2 predicted protein (RefSeq) compare
b4460 araH +0.3 0.8 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b3135 agaA +0.3 1.9 predicted truncated N-acetylgalactosamine-6-phosphate deacetylase (RefSeq) compare
b2486 hyfF +0.3 1.9 NADH dehydrogenase subunit N (NCBI) compare
b2409 yfeR +0.3 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b2945 endA +0.3 1.8 DNA-specific endonuclease I (NCBI) compare
b1345 intR +0.3 2.3 Rac prophage; integrase (NCBI) compare
b1344 ydaO +0.3 0.9 predicted C32 tRNA thiolase (NCBI) compare
b1977 asnT +0.3 1.2 tRNA-Asn (NCBI) compare
b1446 ydcY +0.3 0.7 hypothetical protein (NCBI) compare
b1418 cybB +0.3 0.9 cytochrome b(561) (VIMSS) compare
b0819 ybiS +0.3 2.2 hypothetical protein (NCBI) compare
b1421 trg +0.3 1.4 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b3009 yghB +0.3 1.9 conserved inner membrane protein (NCBI) compare
b0682 ybfN +0.3 1.5 predicted lipoprotein (NCBI) compare
b2399 yfeD +0.3 1.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0193 yaeF +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b4503 yafF +0.3 0.3 no description compare
b1158 pin +0.3 1.7 e14 prophage; site-specific DNA recombinase (NCBI) compare
b0852 rimK +0.3 1.6 ribosomal protein S6 modification protein (NCBI) compare
b1038 csgF +0.3 1.2 predicted transport protein (NCBI) compare
b4360 yjjA +0.3 1.1 putative glycoprotein/receptor (VIMSS) compare
b0713 ybgL +0.3 1.3 hypothetical protein (NCBI) compare
b0641 rlpB +0.3 0.8 minor lipoprotein (NCBI) compare
b3129 sohA +0.3 1.7 predicted regulator (NCBI) compare
b0632 dacA +0.3 2.0 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b4494 arpB +0.3 0.8 no description compare
b2419 yfeK +0.3 1.0 hypothetical protein (NCBI) compare
b4692 oweE +0.3 1.3 no description compare
b4567 yjjZ +0.3 1.9 hypothetical protein (NCBI) compare
b1566 flxA +0.3 1.3 Qin prophage; predicted protein (NCBI) compare
b3686 ibpB +0.3 2.2 heat shock chaperone (NCBI) compare
b4653 yicT +0.3 1.0 no description compare
b3625 rfaY +0.3 1.7 lipopolysaccharide core biosynthesis protein (NCBI) compare
b3396 mrcA +0.3 2.4 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b1959 yedA +0.3 1.3 predicted inner membrane protein (NCBI) compare
b1790 yeaM +0.3 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b0323 yahI +0.3 1.5 predicted carbamate kinase-like protein (NCBI) compare
b4573 insZ +0.3 1.3 no description compare
b2491 hyfR +0.3 1.8 putative 2-component regulator, interaction with sigma 54 (VIMSS) compare
b1097 yceG +0.3 1.6 predicted aminodeoxychorismate lyase (NCBI) compare
b0521 ybcF +0.3 1.8 predicted carbamate kinase (NCBI) compare
b2490 b2490 +0.2 1.6 putative protein processing element (VIMSS) compare
b2743 pcm +0.2 1.7 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2587 kgtP +0.2 1.7 alpha-ketoglutarate transporter (NCBI) compare
b0754 aroG +0.2 1.9 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b1365 ynaK +0.2 1.8 Rac prophage; conserved protein (NCBI) compare
b4656 yjhY +0.2 1.6 no description compare
b3555 yiaG +0.2 1.0 predicted transcriptional regulator (NCBI) compare
b1480 sra +0.2 0.7 30S ribosomal subunit protein S22 (NCBI) compare
b0464 acrR +0.2 2.2 DNA-binding transcriptional repressor (NCBI) compare
b2195 ccmG +0.2 0.7 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b3483 yhhH +0.2 0.1 orf, hypothetical protein (VIMSS) compare
b1764 selD +0.2 1.3 selenophosphate synthetase (NCBI) compare
b2284 nuoF +0.2 1.6 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1677 lpp +0.2 0.8 murein lipoprotein (NCBI) compare
b2445 yffN +0.2 0.8 CPZ-55 prophage; predicted protein (RefSeq) compare
b2622 intA +0.2 1.7 CP4-57 prophage; integrase (NCBI) compare
b0393 rdgC +0.2 1.9 recombination associated protein (NCBI) compare
b4280 yjhC +0.2 1.9 putative dehydrogenase (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms