Experiment set41IT063 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time1-2hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -5.9 -19.3 alanine racemase (NCBI) compare
b4237 nrdG -4.7 -9.0 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -4.7 -14.0 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.2 -5.2 glycerol-3-phosphate acyltransferase (VIMSS) compare
b0949 uup -2.2 -13.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0386 proC -2.0 -5.8 pyrroline-5-carboxylate reductase (NCBI) compare
b2912 ygfA -1.8 -3.8 putative ligase (VIMSS) compare
b2017 yefM -1.7 -1.4 orf, hypothetical protein (VIMSS) compare
b0243 proA -1.7 -10.8 gamma-glutamyl phosphate reductase (NCBI) compare
b1850 eda -1.7 -3.7 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2620 smpB -1.6 -1.7 SsrA-binding protein (NCBI) compare
b4202 rpsR -1.5 -2.8 30S ribosomal protein S18 (NCBI) compare
b0242 proB -1.5 -7.1 gamma-glutamyl kinase (NCBI) compare
b0200 gmhB -1.4 -1.6 hypothetical protein (NCBI) compare
b1346 ydaQ -1.4 -2.5 orf, hypothetical protein (VIMSS) compare
b0232 yafN -1.3 -1.1 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0902 pflA -1.3 -7.0 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b1334 fnr -1.3 -1.7 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2330 yfcB -1.3 -1.4 putative adenine-specific methylase (VIMSS) compare
b0903 pflB -1.1 -9.1 pyruvate formate lyase I (NCBI) compare
b1563 relE -1.1 -2.1 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b2820 recB -1.1 -1.0 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3868 glnG -1.1 -7.1 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b4171 miaA -1.0 -3.7 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0912 ihfB -1.0 -2.6 integration host factor subunit beta (NCBI) compare
b2642 yfjW -1.0 -1.9 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b3781 trxA -1.0 -4.3 thioredoxin 1 (VIMSS) compare
b1984 asnW -0.9 -1.6 tRNA-Asn (NCBI) compare
b0739 tolA -0.9 -2.2 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0990 cspG -0.9 -1.6 DNA-binding transcriptional regulator (NCBI) compare
b1286 rnb -0.9 -1.8 exoribonuclease II (NCBI) compare
b1126 potA -0.8 -5.6 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b1506 yneL -0.8 -1.4 predicted transcriptional regulator (NCBI) compare
b3035 tolC -0.8 -3.5 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3183 obgE -0.8 -1.3 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4535 yniD -0.8 -1.5 predicted protein (RefSeq) compare
b3930 menA -0.8 -7.2 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b0222 lpcA -0.8 -2.3 phosphoheptose isomerase (NCBI) compare
b1124 potC -0.8 -5.1 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2262 menB -0.8 -3.6 naphthoate synthase (NCBI) compare
b2808 gcvA -0.8 -3.0 DNA-binding transcriptional dual regulator (NCBI) compare
b3229 sspA -0.8 -2.1 stringent starvation protein A (NCBI) compare
b2830 nudH -0.8 -1.2 dinucleoside polyphosphate hydrolase (NCBI) compare
b0142 folK -0.7 -0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2659 b2659 -0.7 -3.0 orf, hypothetical protein (VIMSS) compare
b3390 aroK -0.7 -1.8 shikimate kinase I (NCBI) compare
b0014 dnaK -0.7 -2.4 molecular chaperone DnaK (NCBI) compare
b1677 lpp -0.7 -1.8 murein lipoprotein (NCBI) compare
b0688 pgm -0.7 -2.5 phosphoglucomutase (NCBI) compare
b2576 srmB -0.7 -2.6 ATP-dependent RNA helicase (NCBI) compare
b0948 ycbY -0.7 -4.1 predicted methyltransferase (NCBI) compare
b2450 yffS -0.7 -2.7 CPZ-55 prophage; predicted protein (RefSeq) compare
b4436 ryeC -0.7 -1.1 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b3811 xerC -0.7 -0.9 site-specific tyrosine recombinase XerC (NCBI) compare
b2528 iscA -0.7 -1.0 iron-sulfur cluster assembly protein (NCBI) compare
b0167 glnD -0.7 -3.5 PII uridylyl-transferase (NCBI) compare
b4647 mokA -0.7 -1.0 no description compare
b1231 tyrT -0.7 -1.1 tRNA-Tyr (NCBI) compare
b4066 yjcF -0.7 -0.6 hypothetical protein (NCBI) compare
b1486 ddpB -0.7 -2.3 D-ala-D-ala transporter subunit (NCBI) compare
b3405 ompR -0.7 -3.1 osmolarity response regulator (NCBI) compare
b1123 potD -0.7 -3.3 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b1125 potB -0.6 -3.2 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2297 pta -0.6 -0.4 phosphate acetyltransferase (NCBI) compare
b2894 xerD -0.6 -0.5 site-specific tyrosine recombinase XerD (NCBI) compare
b3621 rfaC -0.6 -3.1 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0088 murD -0.6 -2.5 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b1593 b1593 -0.6 -2.2 orf, hypothetical protein (VIMSS) compare
b1482 osmC -0.6 -1.4 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3620 rfaF -0.6 -3.7 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3619 rfaD -0.6 -2.4 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3162 deaD -0.6 -3.0 inducible ATP-independent RNA helicase (VIMSS) compare
b1290 sapF -0.6 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4545 ypdJ -0.6 -2.1 no description compare
b3052 rfaE -0.6 -3.1 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0237 pepD -0.6 -4.0 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b1685 ydiH -0.6 -1.4 predicted protein (RefSeq) compare
b4375 prfC -0.6 -1.8 peptide chain release factor 3 (NCBI) compare
b1147 ymfL -0.6 -1.9 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1292 sapC -0.6 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3761 trpT -0.6 -1.5 tRNA-Trp (NCBI) compare
b0729 sucD -0.6 -2.2 succinyl-CoA synthetase subunit alpha (NCBI) compare
b4493 gapC -0.5 -1.5 no description compare
b1236 galU -0.5 -2.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4589 ylcI -0.5 -1.9 hypothetical protein (RefSeq) compare
b2343 b2343 -0.5 -1.5 orf, hypothetical protein (VIMSS) compare
b0053 surA -0.5 -3.4 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0685 ybfE -0.5 -2.5 orf, hypothetical protein (VIMSS) compare
b1524 yneH -0.5 -0.7 predicted glutaminase (NCBI) compare
b3916 pfkA -0.5 -1.8 6-phosphofructokinase (NCBI) compare
b0234 yafP -0.5 -1.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1862 yebB -0.5 -1.9 orf, hypothetical protein (VIMSS) compare
b4177 purA -0.5 -1.3 adenylosuccinate synthetase (NCBI) compare
b3612 gpmI -0.5 -4.7 phosphoglyceromutase (NCBI) compare
b1088 yceD -0.5 -2.5 hypothetical protein (NCBI) compare
b1659 ydhB -0.5 -2.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b2299 yfcD -0.5 -2.7 predicted NUDIX hydrolase (NCBI) compare
b1108 ycfP -0.5 -1.7 orf, hypothetical protein (VIMSS) compare
b1804 rnd -0.5 -2.3 ribonuclease D (NCBI) compare
b4585 sroB -0.5 -1.0 Novel sRNA, function unknown (RefSeq) compare
b1589 ynfG -0.5 -1.8 oxidoreductase, Fe-S subunit (NCBI) compare
b1258 yciF -0.5 -0.4 hypothetical protein (NCBI) compare
b0740 tolB -0.5 -1.3 translocation protein TolB precursor (NCBI) compare
b1279 yciS -0.5 -0.9 conserved inner membrane protein (NCBI) compare
b2501 ppk -0.5 -3.5 polyphosphate kinase (NCBI) compare
b4593 ymgI -0.5 -0.6 hypothetical protein (RefSeq) compare
b1613 manA -0.5 -1.4 mannose-6-phosphate isomerase (VIMSS) compare
b0741 pal -0.5 -1.6 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b1675 ydhZ -0.5 -2.3 hypothetical protein (NCBI) compare
b1238 tdk -0.5 -2.8 thymidine kinase (NCBI) compare
b1778 yeaA -0.5 -1.1 methionine sulfoxide reductase B (NCBI) compare
b2479 gcvR -0.5 -3.0 transcriptional regulation of gcv operon (VIMSS) compare
b0767 ybhE -0.5 -2.5 6-phosphogluconolactonase (NCBI) compare
b3609 secB -0.5 -2.7 export protein SecB (NCBI) compare
b3002 yqhA -0.4 -1.1 hypothetical protein (NCBI) compare
b1271 yciK -0.4 -1.6 short chain dehydrogenase (NCBI) compare
b2531 iscR -0.4 -1.3 DNA-binding transcriptional repressor (NCBI) compare
b3741 gidA -0.4 -1.5 glucose-inhibited division protein A (NCBI) compare
b3204 ptsN -0.4 -2.4 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3728 pstS -0.4 -2.1 phosphate transporter subunit (NCBI) compare
b4402 yjjY -0.4 -0.5 hypothetical protein (NCBI) compare
b4435 isrC -0.4 -1.5 unknown RNA (NCBI) compare
b1735 chbR -0.4 -1.9 DNA-binding transcriptional dual regulator (NCBI) compare
b1861 ruvA -0.4 -0.4 Holliday junction DNA helicase motor protein (NCBI) compare
b3842 rfaH -0.4 -2.4 transcriptional activator RfaH (NCBI) compare
b0890 ftsK -0.4 -1.4 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3493 pitA -0.4 -2.9 phosphate transporter, low-affinity (NCBI) compare
b1522 yneF -0.4 -1.7 predicted diguanylate cyclase (NCBI) compare
b3026 qseC -0.4 -1.5 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0783 moaC -0.4 -2.2 molybdenum cofactor biosynthesis protein C (NCBI) compare
b0937 ssuE -0.4 -0.9 NAD(P)H-dependent FMN reductase (NCBI) compare
b0975 hyaD -0.4 -2.5 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b0226 dinJ -0.4 -1.3 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3616 tdh -0.4 -2.7 L-threonine 3-dehydrogenase (NCBI) compare
b1291 sapD -0.4 -0.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3149 yraO -0.4 -2.4 DnaA initiator-associating factor for replication initiation (NCBI) compare
b2047 wcaJ -0.4 -2.1 predicted UDP-glucose lipid carrier transferase (NCBI) compare
b2704 srlB -0.4 -1.7 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b1518 lsrG -0.4 -1.0 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b0097 yacA -0.4 -1.5 orf, hypothetical protein (VIMSS) compare
b2652 ileY -0.4 -0.5 tRNA-Ile (NCBI) compare
b0303 ykgI -0.4 -0.5 orf, hypothetical protein (VIMSS) compare
b1754 b1754 -0.4 -1.3 orf, hypothetical protein (VIMSS) compare
b1438 ydcQ -0.4 -2.1 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0737 tolQ -0.4 -0.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2114 metG -0.4 -1.5 methionyl-tRNA synthetase (NCBI) compare
b3617 kbl -0.4 -2.8 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b0357 yaiN -0.4 -1.0 putative alpha helix chain (VIMSS) compare
b2318 truA -0.4 -1.3 tRNA pseudouridine synthase A (NCBI) compare
b1931 yedK -0.4 -2.5 hypothetical protein (NCBI) compare
b0999 cbpM -0.4 -1.4 modulator of CbpA co-chaperone (NCBI) compare
b1539 ydfG -0.4 -2.2 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b2646 ypjF -0.4 -0.9 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b4025 pgi -0.4 -2.9 glucose-6-phosphate isomerase (NCBI) compare
b1605 ydgI -0.4 -2.7 predicted arginine/ornithine antiporter transporter (NCBI) compare
b3600 mtlD -0.4 -2.9 mannitol-1-phosphate 5-dehydrogenase (NCBI) compare
b1726 yniB -0.4 -1.3 predicted inner membrane protein (NCBI) compare
b3839 tatC -0.4 -2.2 TatABCE protein translocation system subunit (NCBI) compare
b0881 clpS -0.4 -1.2 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b4699 fnrS -0.4 -0.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b0118 acnB -0.4 -0.5 aconitate hydratase (NCBI) compare
b3163 nlpI -0.4 -1.9 hypothetical protein (NCBI) compare
b2024 hisA -0.4 -1.6 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b2259 pmrD -0.4 -0.7 polymyxin resistance protein B (VIMSS) compare
b0338 cynR -0.4 -2.9 DNA-binding transcriptional dual regulator (NCBI) compare
b0976 hyaE -0.4 -2.2 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b1196 ycgY -0.4 -1.6 hypothetical protein (NCBI) compare
b1540 ydfH -0.4 -1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1594 dgsA -0.4 -1.3 DNA-binding transcriptional repressor (NCBI) compare
b1503 ydeR -0.4 -1.4 predicted fimbrial-like adhesin protein (NCBI) compare
b4578 insP -0.4 -1.4 no description compare
b0603 ybdO -0.4 -0.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2698 recX -0.3 -1.2 RecA regulator RecX (NCBI) compare
b1953 b1953 -0.3 -1.5 orf, hypothetical protein (VIMSS) compare
b2285 nuoE -0.3 -1.4 NADH dehydrogenase subunit E (NCBI) compare
b1159 mcrA -0.3 -0.6 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2990 hybG -0.3 -1.8 hydrogenase 2 accessory protein (NCBI) compare
b4702 mgtL -0.3 -1.2 regulatory leader peptide for mgtA (NCBI) compare
b0564 appY -0.3 -0.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1004 wrbA -0.3 -1.7 TrpR binding protein WrbA (NCBI) compare
b1837 yebW -0.3 -1.0 orf, hypothetical protein (VIMSS) compare
b1488 ddpX -0.3 -2.0 D-ala-D-ala dipeptidase, Zn-dependent (NCBI) compare
b0938 ycbQ -0.3 -2.4 putative fimbrial-like protein (VIMSS) compare
b0705 ybfL -0.3 -0.2 putative receptor protein (VIMSS) compare
b2038 rfbC -0.3 -2.5 dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI) compare
b1628 rsxB -0.3 -1.5 electron transport complex protein RnfB (NCBI) compare
b1617 uidA -0.3 -2.1 beta-D-glucuronidase (NCBI) compare
b0560 nohB -0.3 -1.2 DLP12 prophage; DNA packaging protein (NCBI) compare
b4462 ygaQ -0.3 -0.9 hypothetical protein (RefSeq) compare
b1160 elbA -0.3 -0.4 hypothetical protein (NCBI) compare
b0728 sucC -0.3 -2.1 succinyl-CoA synthetase subunit beta (NCBI) compare
b2953 yggU -0.3 -1.3 orf, hypothetical protein (VIMSS) compare
b1860 ruvB -0.3 -0.4 Holliday junction DNA helicase B (NCBI) compare
b1712 ihfA -0.3 -0.3 integration host factor subunit alpha (NCBI) compare
b1689 b1689 -0.3 -1.5 orf, hypothetical protein (VIMSS) compare
b1640 ydhH -0.3 -1.7 anhydro-N-acetylmuramic acid kinase (NCBI) compare
b3497 yhiQ -0.3 -2.4 predicted SAM-dependent methyltransferase (NCBI) compare
b4224 chpS -0.3 -2.2 suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS) compare
b1612 fumA -0.3 -1.8 fumarate hydratase (fumarase A), aerobic Class I (NCBI) compare
b1302 puuE -0.3 -1.6 GABA aminotransferase, PLP-dependent (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms