Experiment set41IT058 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time7hr

200 most detrimental genes:

  gene name fitness t score description  
b2934 cmtB +1.2 1.0 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b0630 lipB +1.2 2.2 protein of lipoate biosynthesis (VIMSS) compare
b1185 dsbB +1.2 7.0 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b3860 dsbA +0.9 8.3 periplasmic protein disulfide isomerase I (NCBI) compare
b0798 ybiA +0.8 2.3 hypothetical protein (NCBI) compare
b1195 ymgE +0.8 2.3 predicted inner membrane protein (NCBI) compare
b1348 lar +0.7 2.1 Rac prophage; restriction alleviation protein (NCBI) compare
b1476 fdnI +0.7 1.3 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b1332 ynaJ +0.7 0.8 predicted inner membrane protein (NCBI) compare
b1506 yneL +0.7 1.7 predicted transcriptional regulator (NCBI) compare
b1831 proQ +0.6 3.1 putative solute/DNA competence effector (NCBI) compare
b4045 yjbJ +0.6 2.7 predicted stress response protein (NCBI) compare
b1361 ydaW +0.6 0.5 orf, hypothetical protein (VIMSS) compare
b4418 sraB +0.6 1.3 unknown RNA (NCBI) compare
b1739 osmE +0.6 2.8 DNA-binding transcriptional activator (NCBI) compare
b4480 hdfR +0.6 4.5 transcriptional regulator HdfR (NCBI) compare
b1882 cheY +0.6 0.7 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b4520 ymgF +0.6 1.6 hypothetical protein (NCBI) compare
b2616 recN +0.5 4.5 recombination and repair protein (NCBI) compare
b1954 dsrA +0.5 2.6 regulatory antisense RNA (NCBI) compare
b2209 eco +0.5 1.3 ecotin precursor (NCBI) compare
b3512 GadE +0.5 3.4 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1610 tus +0.5 3.1 DNA replication terminus site-binding protein (NCBI) compare
b0998 torD +0.5 1.2 chaperone protein TorD (NCBI) compare
b1179 ycgL +0.5 2.6 conserved protein (NCBI) compare
b1205 ychH +0.5 1.7 predicted inner membrane protein (NCBI) compare
b2156 lysP +0.5 4.4 lysine transporter (NCBI) compare
b3764 yifE +0.5 3.5 hypothetical protein (NCBI) compare
b1357 ydaS +0.5 1.6 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4676 yoaK +0.5 0.5 expressed protein (RefSeq) compare
b1387 maoC +0.5 2.8 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1382 ynbE +0.5 1.6 predicted lipoprotein (NCBI) compare
b2915 yqfE +0.5 2.4 hypothetical protein (RefSeq) compare
b1824 yobF +0.5 2.5 hypothetical protein (NCBI) compare
b1418 cybB +0.5 2.0 cytochrome b(561) (VIMSS) compare
b1448 yncA +0.5 1.2 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0730 mngR +0.5 1.0 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1823 cspC +0.5 2.0 stress protein, member of the CspA-family (NCBI) compare
b1724 ydiZ +0.5 2.2 hypothetical protein (NCBI) compare
b4571 wbbL +0.5 2.4 no description compare
b4431 rprA +0.5 1.4 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b0542 renD +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b3082 ygjM +0.5 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b4505 insX +0.5 1.1 predicted IS protein (RefSeq) compare
b1434 ydcN +0.5 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1936 intG +0.4 0.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1058 yceO +0.4 0.2 hypothetical protein (NCBI) compare
b1950 fliR +0.4 2.1 flagellar biosynthesis protein R (NCBI) compare
b4642 yoeD +0.4 1.7 no description compare
b1408 b1408 +0.4 3.0 probable enzyme (VIMSS) compare
b0641 rlpB +0.4 1.4 minor lipoprotein (NCBI) compare
b4631 ykgQ +0.4 1.7 no description compare
b0183 rnhB +0.4 2.7 ribonuclease HII (NCBI) compare
b1442 ydcU +0.4 2.8 predicted spermidine/putrescine transporter subunit (NCBI) compare
b3863 polA +0.4 0.7 DNA polymerase I (NCBI) compare
b1415 aldA +0.4 3.2 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b1485 ddpC +0.4 2.5 D-ala-D-ala transporter subunit (NCBI) compare
b0843 ybjH +0.4 1.4 hypothetical protein (NCBI) compare
b2195 ccmG +0.4 2.0 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1566 flxA +0.4 1.9 Qin prophage; predicted protein (NCBI) compare
b1305 pspB +0.4 1.7 phage shock protein B (NCBI) compare
b1613 manA +0.4 1.5 mannose-6-phosphate isomerase (VIMSS) compare
b2998 yghW +0.4 2.5 hypothetical protein (NCBI) compare
b4410 ecnA +0.4 1.2 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1395 paaH +0.4 3.3 3-hydroxybutyryl-CoA dehydrogenase (NCBI) compare
b2673 nrdH +0.4 2.4 glutaredoxin-like protein (NCBI) compare
b1152 ymfP +0.4 2.5 orf, hypothetical protein (VIMSS) compare
b0191 yaeJ +0.4 2.8 hypothetical protein (NCBI) compare
b1753 ynjA +0.4 1.6 hypothetical protein (NCBI) compare
b1238 tdk +0.4 2.7 thymidine kinase (NCBI) compare
b1421 trg +0.4 2.8 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b1478 adhP +0.4 1.6 alcohol dehydrogenase (VIMSS) compare
b1160 elbA +0.4 0.6 hypothetical protein (NCBI) compare
b1742 b1742 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b0623 cspE +0.4 1.2 cold shock protein E (NCBI) compare
b2741 rpoS +0.4 2.7 RNA polymerase sigma factor (NCBI) compare
b4546 ypeB +0.4 0.9 hypothetical protein (NCBI) compare
b1686 ydiI +0.4 1.2 hypothetical protein (NCBI) compare
b4686 yshB +0.4 2.1 expressed protein (RefSeq) compare
b1045 ymdB +0.4 2.3 hypothetical protein (NCBI) compare
b1731 cedA +0.4 1.4 cell division modulator (RefSeq) compare
b1394 paaG +0.4 2.1 enoyl-CoA hydratase (NCBI) compare
b3961 oxyR +0.4 3.1 DNA-binding transcriptional dual regulator (NCBI) compare
b4593 ymgI +0.3 0.4 hypothetical protein (RefSeq) compare
b4515 ybgT +0.3 1.2 hypothetical protein (NCBI) compare
b1977 asnT +0.3 1.6 tRNA-Asn (NCBI) compare
b3627 rfaI +0.3 2.8 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1233 ychJ +0.3 2.4 hypothetical protein (NCBI) compare
b4532 yncN +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1930 yedF +0.3 1.9 hypothetical protein (NCBI) compare
b2261 menC +0.3 2.5 O-succinylbenzoate synthase (NCBI) compare
b3790 rffC +0.3 2.4 TDP-fucosamine acetyltransferase (RefSeq) compare
b4551 yheV +0.3 1.4 hypothetical protein (NCBI) compare
b4640 yoeG +0.3 1.2 no description compare
b1414 ydcF +0.3 2.4 hypothetical protein (NCBI) compare
b2217 rcsB +0.3 2.6 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b4460 araH +0.3 1.2 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1397 paaJ +0.3 2.8 acetyl-CoA acetyltransferase (NCBI) compare
b3037 ygiB +0.3 2.5 conserved outer membrane protein (RefSeq) compare
b1383 ydbL +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b1480 sra +0.3 0.8 30S ribosomal subunit protein S22 (NCBI) compare
b0946 ycbW +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b2016 yeeZ +0.3 2.1 predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b1143 ymfI +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b2071 yegJ +0.3 1.2 hypothetical protein (NCBI) compare
b2433 b2433 +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b2684 mprA +0.3 1.9 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b4438 cyaR +0.3 1.4 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b2897 ygfY +0.3 1.4 hypothetical protein (NCBI) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b1431 ydcL +0.3 1.6 predicted lipoprotein (NCBI) compare
b2096 gatY +0.3 1.4 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b1381 ydbH +0.3 2.6 hypothetical protein (NCBI) compare
b0678 nagB +0.3 1.6 glucosamine-6-phosphate deaminase (NCBI) compare
b4613 dinQ +0.3 1.4 Damage inducible, function unknown (RefSeq) compare
b1085 b1085 +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b3625 rfaY +0.3 1.8 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4481 rffT +0.3 2.1 4-alpha-L-fucosyltransferase (NCBI) compare
b1335 ogt +0.3 1.7 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b4609 ryfD +0.3 1.2 Novel sRNA, function unknown (RefSeq) compare
b2587 kgtP +0.3 2.0 alpha-ketoglutarate transporter (NCBI) compare
b4181 yjfI +0.3 1.6 hypothetical protein (NCBI) compare
b0848 ybjM +0.3 2.0 predicted inner membrane protein (NCBI) compare
b1449 yncB +0.3 2.1 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq) compare
b4603 rseX +0.3 0.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b4492 ydbA +0.3 2.4 no description compare
b3787 rffD +0.3 2.6 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1053 mdtG +0.3 1.8 predicted drug efflux system (NCBI) compare
b0178 hlpA +0.3 1.8 periplasmic chaperone (NCBI) compare
b1410 ynbC +0.3 2.0 predicted hydrolase (NCBI) compare
b4369 leuP +0.3 1.1 tRNA-Leu (NCBI) compare
b1507 hipA +0.3 1.3 regulator with hipB (NCBI) compare
b1961 dcm +0.3 0.8 DNA cytosine methylase (NCBI) compare
b1874 cutC +0.3 0.7 copper homeostasis protein (NCBI) compare
b2419 yfeK +0.3 1.3 hypothetical protein (NCBI) compare
b1315 ycjS +0.3 2.1 predicted oxidoreductase, NADH-binding (NCBI) compare
b3238 yhcN +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b1698 ydiR +0.3 1.7 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b4444 rygA +0.3 1.6 unknown RNA (NCBI) compare
b3628 rfaB +0.3 2.4 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b1639 ydhA +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0933 ssuB +0.3 1.3 alkanesulfonate transporter subunit (NCBI) compare
b1339 abgR +0.3 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b0851 nfsA +0.3 1.6 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b0645 ybeR +0.3 2.1 hypothetical protein (NCBI) compare
b4687 shoB +0.3 1.4 toxic membrane protein (RefSeq) compare
b2765 ygcM +0.3 1.1 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b0122 yacC +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1390 paaC +0.3 1.8 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b2208 napF +0.3 1.5 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b3786 rffE +0.3 2.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2636 b2636 +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b1399 paaX +0.3 1.7 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b4699 fnrS +0.3 0.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1384 feaR +0.3 2.0 DNA-binding transcriptional dual regulator (NCBI) compare
b2010 dacD +0.3 1.9 penicillin binding protein 6b (VIMSS) compare
b0521 ybcF +0.3 1.8 predicted carbamate kinase (NCBI) compare
b1184 umuC +0.3 2.2 DNA polymerase V subunit UmuC (NCBI) compare
b3248 yhdE +0.3 2.2 Maf-like protein (NCBI) compare
b1520 b1520 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b1919 yedO +0.3 0.7 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1695 b1695 +0.3 1.6 putative oxidoreductase (VIMSS) compare
b4503 yafF +0.3 0.4 no description compare
b2859 ygeO +0.3 0.9 hypothetical protein (RefSeq) compare
b1795 yeaQ +0.3 1.4 conserved inner membrane protein (NCBI) compare
b0617 citD +0.3 0.7 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2306 hisP +0.3 2.0 histidine/lysine/arginine/ornithine transporter subunit (NCBI) compare
b4389 radA +0.3 2.2 predicted repair protein (NCBI) compare
b2212 alkB +0.3 1.4 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b0819 ybiS +0.3 2.1 hypothetical protein (NCBI) compare
b1976 b1976 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b0613 citG +0.3 1.3 triphosphoribosyl-dephospho-CoA transferase (NCBI) compare
b1407 ydbD +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b3163 nlpI +0.3 1.4 hypothetical protein (NCBI) compare
b0051 ksgA +0.3 1.4 dimethyladenosine transferase (NCBI) compare
b0632 dacA +0.3 1.8 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b3613 yibP +0.3 0.8 putative membrane protein (VIMSS) compare
b1350 recE +0.3 2.0 Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI) compare
b4656 yjhY +0.3 1.4 no description compare
b3009 yghB +0.3 1.9 conserved inner membrane protein (NCBI) compare
b2579 yfiD +0.2 0.8 pyruvate formate lyase subunit (NCBI) compare
b4538 yoeF +0.2 0.7 hypothetical protein (RefSeq) compare
b1727 yniC +0.2 1.1 predicted hydrolase (NCBI) compare
b4345 mcrC +0.2 0.5 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b0213 yafS +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b0101 yacG +0.2 1.4 zinc-binding protein (NCBI) compare
b1945 fliM +0.2 1.8 flagellar motor switch protein M (NCBI) compare
b1377 ompN +0.2 1.6 outer membrane pore protein N, non-specific (NCBI) compare
b2997 hybO +0.2 1.9 hydrogenase 2, small subunit (NCBI) compare
b2870 ygeW +0.2 1.9 hypothetical protein (NCBI) compare
b1386 tynA +0.2 1.6 tyramine oxidase, copper-requiring (NCBI) compare
b0464 acrR +0.2 2.1 DNA-binding transcriptional repressor (NCBI) compare
b4218 ytfL +0.2 1.8 predicted inner membrane protein (NCBI) compare
b2200 ccmB +0.2 1.6 heme exporter subunit (NCBI) compare
b3791 rffA +0.2 1.8 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2486 hyfF +0.2 1.9 NADH dehydrogenase subunit N (NCBI) compare
b1424 mdoD +0.2 1.7 glucan biosynthesis protein, periplasmic (RefSeq) compare
b3686 ibpB +0.2 2.0 heat shock chaperone (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms