Experiment set41IT054 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time4hr

200 most important genes:

  gene name fitness t score description  
b4053 alr -6.7 -15.7 alanine racemase (NCBI) compare
b4237 nrdG -5.4 -6.4 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -5.2 -10.5 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4041 plsB -4.8 -4.6 glycerol-3-phosphate acyltransferase (VIMSS) compare
b4025 pgi -3.2 -13.4 glucose-6-phosphate isomerase (NCBI) compare
b1850 eda -2.8 -4.0 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1334 fnr -2.7 -3.1 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b0386 proC -2.2 -6.3 pyrroline-5-carboxylate reductase (NCBI) compare
b0949 uup -2.1 -12.5 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0902 pflA -1.9 -9.7 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b0243 proA -1.8 -12.0 gamma-glutamyl phosphate reductase (NCBI) compare
b0242 proB -1.7 -8.5 gamma-glutamyl kinase (NCBI) compare
b1123 potD -1.6 -7.9 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b4647 mokA -1.6 -1.7 no description compare
b1860 ruvB -1.5 -1.6 Holliday junction DNA helicase B (NCBI) compare
b3916 pfkA -1.5 -4.0 6-phosphofructokinase (NCBI) compare
b3183 obgE -1.5 -1.9 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2830 nudH -1.5 -1.8 dinucleoside polyphosphate hydrolase (NCBI) compare
b1124 potC -1.4 -8.5 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b2820 recB -1.4 -0.9 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1126 potA -1.4 -9.0 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b4593 ymgI -1.4 -1.7 hypothetical protein (RefSeq) compare
b0737 tolQ -1.4 -2.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3930 menA -1.3 -11.3 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b3390 aroK -1.2 -2.4 shikimate kinase I (NCBI) compare
b1236 galU -1.2 -4.8 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1125 potB -1.2 -6.0 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b3229 sspA -1.2 -2.6 stringent starvation protein A (NCBI) compare
b0903 pflB -1.1 -8.0 pyruvate formate lyase I (NCBI) compare
b2330 yfcB -1.1 -1.3 putative adenine-specific methylase (VIMSS) compare
b0688 pgm -1.1 -3.6 phosphoglucomutase (NCBI) compare
b2329 aroC -1.1 -6.1 chorismate synthase (NCBI) compare
b2620 smpB -1.1 -1.4 SsrA-binding protein (NCBI) compare
b3781 trxA -1.1 -4.7 thioredoxin 1 (VIMSS) compare
b4375 prfC -1.1 -3.1 peptide chain release factor 3 (NCBI) compare
b4177 purA -1.1 -2.0 adenylosuccinate synthetase (NCBI) compare
b2912 ygfA -1.0 -2.2 putative ligase (VIMSS) compare
b0728 sucC -1.0 -6.2 succinyl-CoA synthetase subunit beta (NCBI) compare
b1048 mdoG -1.0 -5.9 glucan biosynthesis protein, periplasmic (NCBI) compare
b0014 dnaK -1.0 -3.4 molecular chaperone DnaK (NCBI) compare
b2297 pta -1.0 -0.6 phosphate acetyltransferase (NCBI) compare
b3868 glnG -1.0 -5.9 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b2531 iscR -1.0 -2.6 DNA-binding transcriptional repressor (NCBI) compare
b0912 ihfB -1.0 -2.3 integration host factor subunit beta (NCBI) compare
b0053 surA -1.0 -5.6 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0739 tolA -1.0 -2.1 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2659 b2659 -0.9 -2.8 orf, hypothetical protein (VIMSS) compare
b1551 b1551 -0.9 -0.6 orf, hypothetical protein (VIMSS) compare
b2528 iscA -0.9 -2.5 iron-sulfur cluster assembly protein (NCBI) compare
b0908 aroA -0.9 -6.2 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b0729 sucD -0.9 -4.2 succinyl-CoA synthetase subunit alpha (NCBI) compare
b0303 ykgI -0.9 -0.9 orf, hypothetical protein (VIMSS) compare
b3405 ompR -0.9 -3.8 osmolarity response regulator (NCBI) compare
b3162 deaD -0.9 -4.1 inducible ATP-independent RNA helicase (VIMSS) compare
b1160 elbA -0.9 -1.0 hypothetical protein (NCBI) compare
b2576 srmB -0.9 -3.2 ATP-dependent RNA helicase (NCBI) compare
b4202 rpsR -0.9 -1.7 30S ribosomal protein S18 (NCBI) compare
b3706 trmE -0.9 -3.1 tRNA modification GTPase (NCBI) compare
b3389 aroB -0.8 -6.6 3-dehydroquinate synthase (NCBI) compare
b3341 rpsG -0.8 -1.3 30S ribosomal protein S7 (NCBI) compare
b3281 aroE -0.8 -7.5 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3035 tolC -0.8 -3.4 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b4676 yoaK -0.8 -0.5 expressed protein (RefSeq) compare
b1049 mdoH -0.8 -6.2 glucosyltransferase MdoH (NCBI) compare
b3806 cyaA -0.8 -5.3 adenylate cyclase (NCBI) compare
b0738 tolR -0.8 -1.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4535 yniD -0.8 -1.3 predicted protein (RefSeq) compare
b2569 lepA -0.8 -3.8 GTP-binding protein LepA (NCBI) compare
b2808 gcvA -0.8 -3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1192 ldcA -0.8 -1.8 L,D-carboxypeptidase A (NCBI) compare
b0082 mraW -0.8 -3.7 S-adenosyl-methyltransferase (NCBI) compare
b1293 sapB -0.8 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1853 yebK -0.8 -2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3977 tyrU -0.8 -0.9 tRNA-Tyr (NCBI) compare
b2501 ppk -0.7 -5.7 polyphosphate kinase (NCBI) compare
b1524 yneH -0.7 -1.1 predicted glutaminase (NCBI) compare
b2699 recA -0.7 -0.7 recombinase A (NCBI) compare
b0167 glnD -0.7 -3.2 PII uridylyl-transferase (NCBI) compare
b2110 yehC -0.7 -1.5 predicted periplasmic pilin chaperone (NCBI) compare
b3493 pitA -0.7 -4.9 phosphate transporter, low-affinity (NCBI) compare
b2168 fruK -0.7 -0.8 1-phosphofructokinase (NCBI) compare
b0603 ybdO -0.7 -0.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b0145 dksA -0.7 -2.4 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b1159 mcrA -0.7 -1.2 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b0740 tolB -0.7 -2.0 translocation protein TolB precursor (NCBI) compare
b0237 pepD -0.7 -5.2 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b0118 acnB -0.7 -0.5 aconitate hydratase (NCBI) compare
b1347 ydaC -0.7 -0.4 Rac prophage; predicted protein (NCBI) compare
b1241 adhE -0.7 -4.9 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b3164 pnp -0.7 -2.6 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0741 pal -0.7 -2.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4462 ygaQ -0.7 -1.6 hypothetical protein (RefSeq) compare
b0142 folK -0.7 -0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b2846 yqeH -0.7 -0.5 orf, hypothetical protein (VIMSS) compare
b3620 rfaF -0.7 -3.6 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b2318 truA -0.7 -1.8 tRNA pseudouridine synthase A (NCBI) compare
b1685 ydiH -0.7 -1.7 predicted protein (RefSeq) compare
b1842 holE -0.7 -2.5 DNA polymerase III, theta subunit (NCBI) compare
b1292 sapC -0.6 -1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0080 fruR -0.6 -4.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3204 ptsN -0.6 -2.9 sugar-specific enzyme IIA component of PTS (NCBI) compare
b2343 b2343 -0.6 -2.1 orf, hypothetical protein (VIMSS) compare
b2553 glnB -0.6 -2.3 regulatory protein P-II for glutamine synthetase (NCBI) compare
b0500 ybbD -0.6 -0.3 hypothetical protein (RefSeq) compare
b1536 ydeI -0.6 -0.8 hypothetical protein (NCBI) compare
b2081 yegQ -0.6 -4.7 predicted peptidase (NCBI) compare
b2479 gcvR -0.6 -3.4 transcriptional regulation of gcv operon (VIMSS) compare
b1730 ydjO -0.6 -1.0 predicted protein (RefSeq) compare
b0580 ybdJ -0.6 -2.4 predicted inner membrane protein (NCBI) compare
b0677 nagA -0.6 -3.2 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b2017 yefM -0.6 -0.7 orf, hypothetical protein (VIMSS) compare
b1088 yceD -0.6 -3.1 hypothetical protein (NCBI) compare
b2625 yfjI -0.6 -1.3 CP4-57 prophage; predicted protein (NCBI) compare
b4201 priB -0.6 -2.2 primosomal replication protein N (NCBI) compare
b3728 pstS -0.6 -2.6 phosphate transporter subunit (NCBI) compare
b0549 ybcO -0.6 -1.0 DLP12 prophage; predicted protein (NCBI) compare
b1290 sapF -0.6 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0957 ompA -0.6 -2.9 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b2562 yfhL -0.5 -4.5 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b1550 b1550 -0.5 -0.8 orf, hypothetical protein (VIMSS) compare
b4131 cadA -0.5 -5.0 lysine decarboxylase 1 (NCBI) compare
b1253 yciA -0.5 -2.1 predicted hydrolase (NCBI) compare
b1648 b1648 -0.5 -1.5 orf, hypothetical protein (VIMSS) compare
b0953 rmf -0.5 -0.9 ribosome modulation factor (NCBI) compare
b3247 cafA -0.5 -4.7 bundles of cytoplasmic filaments (VIMSS) compare
b4592 yccB -0.5 -1.6 hypothetical protein (RefSeq) compare
b2007 yeeX -0.5 -2.1 conserved protein (RefSeq) compare
b3884 yihW -0.5 -3.8 putative DEOR-type transcriptional regulator (VIMSS) compare
b2055 wcaE -0.5 -0.3 predicted glycosyl transferase (NCBI) compare
b1863 ruvC -0.5 -0.9 Holliday junction resolvase (NCBI) compare
b1613 manA -0.5 -1.3 mannose-6-phosphate isomerase (VIMSS) compare
b1171 b1171 -0.5 -2.2 orf, hypothetical protein (VIMSS) compare
b3649 rpoZ -0.5 -2.4 DNA-directed RNA polymerase subunit omega (NCBI) compare
b2393 nupC -0.5 -3.3 nucleoside (except guanosine) transporter (NCBI) compare
b2288 nuoA -0.5 -1.7 NADH dehydrogenase I chain A (VIMSS) compare
b1865 nudB -0.5 -1.2 dATP pyrophosphohydrolase (NCBI) compare
b1148 ymfM -0.5 -1.9 e14 prophage; predicted protein (NCBI) compare
b1712 ihfA -0.5 -0.6 integration host factor subunit alpha (NCBI) compare
b2319 usg -0.5 -2.1 hypothetical protein (NCBI) compare
b1497 b1497 -0.5 -0.3 putative enzyme (VIMSS) compare
b2894 xerD -0.5 -0.5 site-specific tyrosine recombinase XerD (NCBI) compare
b4179 vacB -0.5 -3.2 putative enzyme (VIMSS) compare
b2024 hisA -0.5 -1.7 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b1542 ydfI -0.5 -2.8 predicted mannonate dehydrogenase (NCBI) compare
b2652 ileY -0.5 -0.6 tRNA-Ile (NCBI) compare
b4443 gcvB -0.5 -0.2 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b1804 rnd -0.5 -2.3 ribonuclease D (NCBI) compare
b3778 rep -0.5 -3.7 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1505 ydeT -0.5 -0.9 hypothetical protein (RefSeq) compare
b4414 tff -0.5 -1.4 unknown RNA (NCBI) compare
b4178 yjeB -0.5 -3.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2939 yqgB -0.5 -0.9 predicted protein (RefSeq) compare
b1627 rsxA -0.5 -2.6 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b0051 ksgA -0.5 -2.5 dimethyladenosine transferase (NCBI) compare
b4153 frdB -0.5 -3.8 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b2704 srlB -0.5 -1.9 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b1001 yccE -0.5 -0.2 hypothetical protein (NCBI) compare
b0357 yaiN -0.5 -1.6 putative alpha helix chain (VIMSS) compare
b0437 clpP -0.5 -2.7 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1258 yciF -0.5 -0.6 hypothetical protein (NCBI) compare
b0890 ftsK -0.5 -1.7 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3158 yhbU -0.5 -3.8 predicted peptidase (collagenase-like) (NCBI) compare
b2853 ygeI -0.5 -0.2 hypothetical protein (NCBI) compare
b2948 yqgE -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b4402 yjjY -0.4 -0.5 hypothetical protein (NCBI) compare
b2911 ssrS -0.4 -1.3 6S regulatory RNA (NCBI) compare
b1593 b1593 -0.4 -2.8 orf, hypothetical protein (VIMSS) compare
b4132 cadB -0.4 -4.1 predicted lysine/cadaverine transporter (NCBI) compare
b4171 miaA -0.4 -1.8 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1165 ymgA -0.4 -0.2 hypothetical protein (NCBI) compare
b1167 ymgC -0.4 -0.2 hypothetical protein (NCBI) compare
b2285 nuoE -0.4 -1.3 NADH dehydrogenase subunit E (NCBI) compare
b0450 glnK -0.4 -1.3 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b3180 yhbY -0.4 -2.7 predicted RNA-binding protein (NCBI) compare
b0149 mrcB -0.4 -3.7 penicillin-binding protein 1b (NCBI) compare
b1754 b1754 -0.4 -1.6 orf, hypothetical protein (VIMSS) compare
b3157 yhbT -0.4 -2.6 predicted lipid carrier protein (NCBI) compare
b2784 relA -0.4 -3.2 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b1346 ydaQ -0.4 -1.0 orf, hypothetical protein (VIMSS) compare
b1563 relE -0.4 -1.6 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b0746 valZ -0.4 -1.6 tRNA-Val (NCBI) compare
b3159 yhbV -0.4 -3.4 orf, hypothetical protein (VIMSS) compare
b4545 ypdJ -0.4 -1.6 no description compare
b1493 gadB -0.4 -0.7 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b0948 ycbY -0.4 -2.6 predicted methyltransferase (NCBI) compare
b0975 hyaD -0.4 -2.2 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b1412 azoR -0.4 -2.1 acyl carrier protein phosphodiesterase (NCBI) compare
b3210 arcB -0.4 -2.6 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b0990 cspG -0.4 -0.7 DNA-binding transcriptional regulator (NCBI) compare
b1235 rssB -0.4 -2.5 response regulator of RpoS (NCBI) compare
b1677 lpp -0.4 -1.1 murein lipoprotein (NCBI) compare
b4451 ryhB -0.4 -1.6 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b3612 gpmI -0.4 -3.8 phosphoglyceromutase (NCBI) compare
b0234 yafP -0.4 -1.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0683 fur -0.4 -0.9 ferric uptake regulator (NCBI) compare
b1576 ydfD -0.4 -0.2 Qin prophage; predicted protein (NCBI) compare
b2847 yqeI -0.4 -0.4 predicted transcriptional regulator (NCBI) compare
b1630 rsxD -0.4 -2.1 electron transport complex protein RnfD (NCBI) compare
b1429 tehA -0.4 -0.9 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1739 osmE -0.4 -1.2 DNA-binding transcriptional activator (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms