Experiment set41IT054 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time4hr

200 most detrimental genes:

  gene name fitness t score description  
b2934 cmtB +1.5 1.3 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1361 ydaW +1.1 0.9 orf, hypothetical protein (VIMSS) compare
b0798 ybiA +0.9 3.5 hypothetical protein (NCBI) compare
b0730 mngR +0.9 1.9 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4533 ynfO +0.9 2.4 Qin prophage; predicted protein (NCBI) compare
b1882 cheY +0.8 0.9 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1332 ynaJ +0.7 0.8 predicted inner membrane protein (NCBI) compare
b4603 rseX +0.7 0.9 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b0542 renD +0.6 2.4 orf, hypothetical protein (VIMSS) compare
b4699 fnrS +0.6 1.0 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1195 ymgE +0.6 2.2 predicted inner membrane protein (NCBI) compare
b0630 lipB +0.6 1.1 protein of lipoate biosynthesis (VIMSS) compare
b1861 ruvA +0.6 1.1 Holliday junction DNA helicase motor protein (NCBI) compare
b1185 dsbB +0.6 3.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1765 ydjA +0.6 1.0 predicted oxidoreductase (NCBI) compare
b1143 ymfI +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b1476 fdnI +0.6 1.0 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b3860 dsbA +0.6 5.2 periplasmic protein disulfide isomerase I (NCBI) compare
b4701 sokX +0.6 1.1 sok-related sRNA, function unknown (RefSeq) compare
b4503 yafF +0.6 1.0 no description compare
b0880 cspD +0.5 2.1 cold shock protein homolog (NCBI) compare
b1348 lar +0.5 1.4 Rac prophage; restriction alleviation protein (NCBI) compare
b4538 yoeF +0.5 1.1 hypothetical protein (RefSeq) compare
b4410 ecnA +0.5 1.4 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b3863 polA +0.5 0.7 DNA polymerase I (NCBI) compare
b4642 yoeD +0.5 1.2 no description compare
b1506 yneL +0.5 1.0 predicted transcriptional regulator (NCBI) compare
b3790 rffC +0.5 3.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b1831 proQ +0.4 1.8 putative solute/DNA competence effector (NCBI) compare
b1848 yebG +0.4 1.2 conserved protein regulated by LexA (NCBI) compare
b1357 ydaS +0.4 1.4 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1530 marR +0.4 1.9 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b4431 rprA +0.4 1.6 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1085 b1085 +0.4 1.1 orf, hypothetical protein (VIMSS) compare
b1758 b1758 +0.4 1.0 putative cytochrome oxidase (VIMSS) compare
b1977 asnT +0.4 1.7 tRNA-Asn (NCBI) compare
b4686 yshB +0.4 2.4 expressed protein (RefSeq) compare
b1205 ychH +0.4 1.4 predicted inner membrane protein (NCBI) compare
b4481 rffT +0.4 2.9 4-alpha-L-fucosyltransferase (NCBI) compare
b0843 ybjH +0.4 1.2 hypothetical protein (NCBI) compare
b1573 ydfC +0.4 0.5 Qin prophage; predicted protein (RefSeq) compare
b3791 rffA +0.4 2.8 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1686 ydiI +0.4 0.9 hypothetical protein (NCBI) compare
b3627 rfaI +0.4 3.1 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3623 rfaK +0.4 1.3 lipopolysaccharide core biosynthesis (NCBI) compare
b2510 b2510 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b1811 yoaH +0.4 2.0 hypothetical protein (NCBI) compare
b1175 minD +0.4 1.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b0946 ycbW +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b1565 ydfV +0.4 1.8 Qin prophage; predicted protein (NCBI) compare
b1355 b1355 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1698 ydiR +0.4 1.6 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b2445 yffN +0.4 1.2 CPZ-55 prophage; predicted protein (RefSeq) compare
b1252 tonB +0.4 1.5 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b4416 rybA +0.4 1.0 unknown RNA (NCBI) compare
b0846 ybjK +0.4 2.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1727 yniC +0.4 1.1 predicted hydrolase (NCBI) compare
b1566 flxA +0.3 1.3 Qin prophage; predicted protein (NCBI) compare
b3794 rffM +0.3 2.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1426 ydcH +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2504 yfgG +0.3 0.8 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b0183 rnhB +0.3 2.3 ribonuclease HII (NCBI) compare
b4045 yjbJ +0.3 1.7 predicted stress response protein (NCBI) compare
b2058 wcaB +0.3 0.8 predicted acyl transferase (NCBI) compare
b4360 yjjA +0.3 2.1 putative glycoprotein/receptor (VIMSS) compare
b3979 thrT +0.3 0.1 tRNA-Thr (NCBI) compare
b3250 mreC +0.3 1.3 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b4660 yhiL +0.3 0.2 no description compare
b0323 yahI +0.3 2.1 predicted carbamate kinase-like protein (NCBI) compare
b4704 arrS +0.3 0.1 Antisense sRNA ArrS, function unknown (NCBI) compare
b2398 yfeC +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b4551 yheV +0.3 1.2 hypothetical protein (NCBI) compare
b1764 selD +0.3 2.0 selenophosphate synthetase (NCBI) compare
b2187 yejL +0.3 1.2 hypothetical protein (NCBI) compare
b1387 maoC +0.3 1.8 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b3786 rffE +0.3 2.8 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1959 yedA +0.3 1.9 predicted inner membrane protein (NCBI) compare
b1930 yedF +0.3 2.2 hypothetical protein (NCBI) compare
b0456 ybaA +0.3 1.0 hypothetical protein (NCBI) compare
b3483 yhhH +0.3 0.1 orf, hypothetical protein (VIMSS) compare
b2165 yeiN +0.3 1.8 hypothetical protein (NCBI) compare
b1399 paaX +0.3 1.8 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b1179 ycgL +0.3 1.2 conserved protein (NCBI) compare
b4415 hokE +0.3 1.9 toxic polypeptide, small (NCBI) compare
b1152 ymfP +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b1408 b1408 +0.3 1.6 probable enzyme (VIMSS) compare
b1557 cspB +0.3 0.8 Qin prophage; cold shock protein (NCBI) compare
b4692 oweE +0.3 1.1 no description compare
b0209 yafD +0.3 2.3 hypothetical protein (NCBI) compare
b2264 menD +0.3 2.6 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b2356 yfdM +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b2419 yfeK +0.3 1.0 hypothetical protein (NCBI) compare
b0802 ybiJ +0.3 1.5 hypothetical protein (NCBI) compare
b4527 b1354 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b0819 ybiS +0.3 2.3 hypothetical protein (NCBI) compare
b2193 narP +0.3 2.0 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b0296 ykgM +0.3 1.7 50S ribosomal protein L31 (NCBI) compare
b1792 yeaO +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2587 kgtP +0.3 1.8 alpha-ketoglutarate transporter (NCBI) compare
b3961 oxyR +0.3 2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b2208 napF +0.3 1.4 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b2399 yfeD +0.3 1.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1590 ynfH +0.3 1.9 oxidoreductase, membrane subunit (NCBI) compare
b1103 ycfF +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b1951 rcsA +0.3 0.7 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b1790 yeaM +0.3 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b1954 dsrA +0.3 1.2 regulatory antisense RNA (NCBI) compare
b0393 rdgC +0.3 2.3 recombination associated protein (NCBI) compare
b2051 wcaH +0.3 0.9 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2488 hyfH +0.3 1.3 hydrogenase 4, Fe-S subunit (NCBI) compare
b1511 ydeV +0.3 1.2 putative kinase (VIMSS) compare
b0381 ddlA +0.3 2.2 D-alanylalanine synthetase (NCBI) compare
b3784 rfe +0.3 2.4 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b2287 nuoB +0.3 1.3 NADH dehydrogenase subunit B (NCBI) compare
b0114 aceE +0.3 0.6 pyruvate dehydrogenase subunit E1 (NCBI) compare
b4444 rygA +0.3 1.3 unknown RNA (NCBI) compare
b1520 b1520 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b4363 yjjB +0.3 1.4 conserved inner membrane protein (RefSeq) compare
b4609 ryfD +0.3 1.6 Novel sRNA, function unknown (RefSeq) compare
b1562 hokD +0.3 1.2 Qin prophage; small toxic polypeptide (NCBI) compare
b0521 ybcF +0.3 1.5 predicted carbamate kinase (NCBI) compare
b1114 mfd +0.3 2.1 transcription-repair coupling factor (NCBI) compare
b2782 chpA +0.3 1.6 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b0101 yacG +0.3 0.9 zinc-binding protein (NCBI) compare
b3698 yidB +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1948 fliP +0.3 1.7 flagellar biosynthesis protein P (NCBI) compare
b1075 flgD +0.3 1.3 flagellar basal body rod modification protein D (NCBI) compare
b4395 ytjC +0.3 1.7 phosphoglycerate mutase (NCBI) compare
b4558 yifL +0.3 1.3 predicted lipoprotein (NCBI) compare
b3100 yqjK +0.3 1.8 hypothetical protein (NCBI) compare
b1260 trpA +0.3 1.8 tryptophan synthase subunit alpha (NCBI) compare
b4331 yjiI +0.3 2.0 orf, hypothetical protein (VIMSS) compare
b1013 ycdC +0.3 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2486 hyfF +0.3 1.7 NADH dehydrogenase subunit N (NCBI) compare
b1751 ydjY +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b3950 frwB +0.3 1.1 predicted enzyme IIB component of PTS (NCBI) compare
b2023 hisH +0.3 1.5 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b4000 hupA +0.2 1.3 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b2388 glk +0.2 1.6 glucokinase (NCBI) compare
b2014 yeeF +0.2 1.7 putative amino acid/amine transport protein (VIMSS) compare
b1038 csgF +0.2 1.2 predicted transport protein (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b3163 nlpI +0.2 1.5 hypothetical protein (NCBI) compare
b0641 rlpB +0.2 1.2 minor lipoprotein (NCBI) compare
b4381 deoC +0.2 1.2 deoxyribose-phosphate aldolase (NCBI) compare
b2156 lysP +0.2 2.1 lysine transporter (NCBI) compare
b4367 fhuF +0.2 1.9 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b1034 ycdX +0.2 1.6 hypothetical protein (NCBI) compare
b4614 sokA +0.2 1.2 no description compare
b4032 malG +0.2 1.9 maltose transporter subunit (NCBI) compare
b1699 ydiS +0.2 1.1 predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI) compare
b4034 malE +0.2 2.0 maltose ABC transporter periplasmic protein (NCBI) compare
b1325 ycjG +0.2 1.1 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b1345 intR +0.2 2.0 Rac prophage; integrase (NCBI) compare
b4445 omrB +0.2 1.0 sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq) compare
b1233 ychJ +0.2 1.4 hypothetical protein (NCBI) compare
b4656 yjhY +0.2 1.4 no description compare
b1446 ydcY +0.2 1.1 hypothetical protein (NCBI) compare
b1515 lsrD +0.2 1.0 AI2 transporter (NCBI) compare
b2673 nrdH +0.2 1.4 glutaredoxin-like protein (NCBI) compare
b3889 yiiE +0.2 0.9 predicted transcriptional regulator (RefSeq) compare
b0192 nlpE +0.2 1.7 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b3686 ibpB +0.2 1.7 heat shock chaperone (NCBI) compare
b1299 puuR +0.2 1.6 DNA-binding transcriptional repressor (NCBI) compare
b3787 rffD +0.2 1.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1462 b1462 +0.2 0.6 orf, hypothetical protein (VIMSS) compare
b4573 insZ +0.2 1.3 no description compare
b0443 ybaW +0.2 1.7 hypothetical protein (NCBI) compare
b1308 pspE +0.2 1.6 thiosulfate:cyanide sulfurtransferase (rhodanese) (NCBI) compare
b1955 yedP +0.2 1.5 hypothetical protein (NCBI) compare
b1940 fliH +0.2 1.6 flagellar biosynthesis; export of flagellar proteins? (VIMSS) compare
b1753 ynjA +0.2 1.0 hypothetical protein (NCBI) compare
b3396 mrcA +0.2 2.2 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b1794 yeaP +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b4358 yjjN +0.2 1.8 predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq) compare
b2998 yghW +0.2 1.3 hypothetical protein (NCBI) compare
b3472 b3472 +0.2 1.9 orf, hypothetical protein (VIMSS) compare
b1553 ydfP +0.2 0.6 Qin prophage; conserved protein (NCBI) compare
b4519 icdC +0.2 1.2 no description compare
b3291 mscL +0.2 1.8 large-conductance mechanosensitive channel (NCBI) compare
b2426 ucpA +0.2 0.9 putative oxidoreductase (VIMSS) compare
b1742 b1742 +0.2 0.5 orf, hypothetical protein (VIMSS) compare
b1883 cheB +0.2 1.0 chemotaxis-specific methylesterase (NCBI) compare
b1749 xthA +0.2 1.1 exonuclease III (NCBI) compare
b4658 yghX +0.2 1.8 no description compare
b1380 ldhA +0.2 1.0 D-lactate dehydrogenase (NCBI) compare
b0611 rna +0.2 1.5 ribonuclease I (NCBI) compare
b2302 yfcG +0.2 1.5 predicted glutathione S-transferase (NCBI) compare
b0606 ahpF +0.2 1.6 alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS) compare
b0571 cusR +0.2 1.7 DNA-binding response regulator in two-component regulatory system with CusS (NCBI) compare
b1962 yedJ +0.2 1.5 predicted phosphohydrolase (NCBI) compare
b0550 rusA +0.2 1.2 DLP12 prophage; endonuclease RUS (NCBI) compare
b3628 rfaB +0.2 1.9 UDP-D-galactose:(glucosyl)lipopolysaccharide-1, 6-D-galactosyltransferase (RefSeq) compare
b4376 osmY +0.2 1.5 periplasmic protein (NCBI) compare
b0756 galM +0.2 1.3 galactose-1-epimerase (mutarotase) (NCBI) compare
b2250 b2250 +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b1591 b1591 +0.2 0.8 putative oxidoreductase component (VIMSS) compare
b2381 ypdB +0.2 1.3 predicted response regulator in two-component system withYpdA (NCBI) compare
b4561 insM +0.2 1.3 transposase (RefSeq) compare
b2481 hyfA +0.2 1.5 hydrogenase 4 Fe-S subunit (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms