Experiment set41IT051 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time1-2hr

200 most detrimental genes:

  gene name fitness t score description  
b2934 cmtB +1.4 1.2 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b0630 lipB +1.1 2.0 protein of lipoate biosynthesis (VIMSS) compare
b1361 ydaW +1.1 0.9 orf, hypothetical protein (VIMSS) compare
b4538 yoeF +0.9 2.7 hypothetical protein (RefSeq) compare
b1160 elbA +0.9 1.5 hypothetical protein (NCBI) compare
b1506 yneL +0.8 1.7 predicted transcriptional regulator (NCBI) compare
b4596 yciZ +0.7 1.7 hypothetical protein (RefSeq) compare
b4603 rseX +0.7 0.9 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1058 yceO +0.7 0.3 hypothetical protein (NCBI) compare
b1195 ymgE +0.7 2.6 predicted inner membrane protein (NCBI) compare
b0802 ybiJ +0.7 3.1 hypothetical protein (NCBI) compare
b1524 yneH +0.6 1.2 predicted glutaminase (NCBI) compare
b1936 intG +0.6 1.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b0880 cspD +0.6 2.3 cold shock protein homolog (NCBI) compare
b1183 umuD +0.6 2.2 DNA polymerase V, subunit D (NCBI) compare
b0798 ybiA +0.6 2.2 hypothetical protein (NCBI) compare
b1357 ydaS +0.5 1.7 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0843 ybjH +0.5 1.5 hypothetical protein (NCBI) compare
b1363 trkG +0.5 0.5 Rac prophage; potassium transporter subunit (NCBI) compare
b4699 fnrS +0.5 0.8 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1257 yciE +0.5 0.3 hypothetical protein (NCBI) compare
b1882 cheY +0.5 0.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b0145 dksA +0.5 1.5 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b4000 hupA +0.5 2.8 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b4410 ecnA +0.5 1.4 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b3027 ygiZ +0.5 0.3 conserved inner membrane protein (NCBI) compare
b1408 b1408 +0.5 2.7 probable enzyme (VIMSS) compare
b1954 dsrA +0.5 1.8 regulatory antisense RNA (NCBI) compare
b2071 yegJ +0.5 1.3 hypothetical protein (NCBI) compare
b1739 osmE +0.4 1.5 DNA-binding transcriptional activator (NCBI) compare
b1575 dicB +0.4 1.1 Qin prophage; cell division inhibition protein (NCBI) compare
b2209 eco +0.4 1.7 ecotin precursor (NCBI) compare
b4585 sroB +0.4 1.1 Novel sRNA, function unknown (RefSeq) compare
b3889 yiiE +0.4 1.8 predicted transcriptional regulator (RefSeq) compare
b1669 ydhT +0.4 2.1 hypothetical protein (NCBI) compare
b2650 ypjC +0.4 0.2 hypothetical protein (RefSeq) compare
b1150 ymfR +0.4 0.8 e14 prophage; predicted protein (NCBI) compare
b4128 yjdK +0.4 0.4 hypothetical protein (NCBI) compare
b1961 dcm +0.4 1.3 DNA cytosine methylase (NCBI) compare
b0730 mngR +0.4 0.8 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4640 yoeG +0.4 1.3 no description compare
b1085 b1085 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b2510 b2510 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1742 b1742 +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1138 ymfE +0.4 1.0 e14 prophage; predicted inner membrane protein (NCBI) compare
b3624 rfaZ +0.4 1.5 lipopolysaccharide core biosynthesis protein (NCBI) compare
b1407 ydbD +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b4546 ypeB +0.4 0.8 hypothetical protein (NCBI) compare
b0521 ybcF +0.4 1.9 predicted carbamate kinase (NCBI) compare
b3207 yrbL +0.4 2.5 hypothetical protein (NCBI) compare
b0714 nei +0.4 2.0 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b1434 ydcN +0.4 0.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b4573 insZ +0.4 2.2 no description compare
b1572 ydfB +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b2935 tktA +0.3 0.8 transketolase 1, thiamin-binding (NCBI) compare
b4651 yibW +0.3 0.3 no description compare
b1348 lar +0.3 0.9 Rac prophage; restriction alleviation protein (NCBI) compare
b2399 yfeD +0.3 2.3 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0114 aceE +0.3 0.8 pyruvate dehydrogenase subunit E1 (NCBI) compare
b0407 yajC +0.3 0.8 preprotein translocase subunit YajC (NCBI) compare
b2432 yfeY +0.3 1.3 hypothetical protein (NCBI) compare
b0310 ykgH +0.3 0.7 predicted inner membrane protein (NCBI) compare
b0632 dacA +0.3 2.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b4686 yshB +0.3 1.7 expressed protein (RefSeq) compare
b2356 yfdM +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b4042 dgkA +0.3 0.2 diacylglycerol kinase (NCBI) compare
b1258 yciF +0.3 0.5 hypothetical protein (NCBI) compare
b1490 yddV +0.3 1.9 predicted diguanylate cyclase (RefSeq) compare
b3623 rfaK +0.3 1.2 lipopolysaccharide core biosynthesis (NCBI) compare
b1799 yeaT +0.3 1.7 putative transcriptional regulator LYSR-type (VIMSS) compare
b1729 ydjN +0.3 1.3 predicted transporter (NCBI) compare
b1205 ychH +0.3 1.1 predicted inner membrane protein (NCBI) compare
b1152 ymfP +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b2488 hyfH +0.3 1.6 hydrogenase 4, Fe-S subunit (NCBI) compare
b3100 yqjK +0.3 1.7 hypothetical protein (NCBI) compare
b4642 yoeD +0.3 1.5 no description compare
b1426 ydcH +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b2200 ccmB +0.3 1.6 heme exporter subunit (NCBI) compare
b1999 yeeP +0.3 2.0 putative histone (VIMSS) compare
b1545 pinQ +0.3 0.1 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1165 ymgA +0.3 0.2 hypothetical protein (NCBI) compare
b0736 ybgC +0.3 1.1 predicted acyl-CoA thioesterase (NCBI) compare
b3250 mreC +0.3 0.7 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b1576 ydfD +0.3 0.1 Qin prophage; predicted protein (NCBI) compare
b1499 ydeO +0.3 0.1 predicted DNA-binding transcriptional acfivator (NCBI) compare
b2181 yejG +0.3 1.2 hypothetical protein (NCBI) compare
b0889 lrp +0.3 1.0 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1727 yniC +0.3 1.0 predicted hydrolase (NCBI) compare
b4660 yhiL +0.3 0.2 no description compare
b4598 yncL +0.3 1.1 hypothetical protein (RefSeq) compare
b1566 flxA +0.3 1.3 Qin prophage; predicted protein (NCBI) compare
b2419 yfeK +0.3 1.1 hypothetical protein (NCBI) compare
b0535 fimZ +0.3 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b3775 ppiC +0.3 1.4 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b1802 yeaW +0.3 1.6 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b0218 yafU +0.3 1.0 predicted inner membrane protein (RefSeq) compare
b4158 yjeO +0.3 1.9 conserved inner membrane protein (NCBI) compare
b2782 chpA +0.3 1.9 toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI) compare
b2587 kgtP +0.3 1.7 alpha-ketoglutarate transporter (NCBI) compare
b1276 acnA +0.3 2.0 aconitate hydratase (NCBI) compare
b1667 ydhR +0.3 1.1 hypothetical protein (NCBI) compare
b1175 minD +0.3 1.6 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1176 minC +0.3 0.9 septum formation inhibitor (NCBI) compare
b4104 phnE +0.3 1.2 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b4505 insX +0.3 0.7 predicted IS protein (RefSeq) compare
b3863 polA +0.3 0.4 DNA polymerase I (NCBI) compare
b2930 yggF +0.3 1.8 predicted hexoseP phosphatase (NCBI) compare
b1691 ydiN +0.3 1.2 predicted transporter (RefSeq) compare
b2293 yfbT +0.3 1.5 putative phosphatase (VIMSS) compare
b4045 yjbJ +0.3 1.1 predicted stress response protein (NCBI) compare
b2617 smpA +0.3 1.5 small membrane protein A (VIMSS) compare
b0946 ycbW +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1251 yciI +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b0934 ycbM +0.3 1.3 putative transport system permease protein (VIMSS) compare
b2058 wcaB +0.3 0.6 predicted acyl transferase (NCBI) compare
b0548 ninE +0.3 1.4 DLP12 prophage; conserved protein (NCBI) compare
b2023 hisH +0.3 1.5 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b0542 renD +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0726 sucA +0.3 1.2 alpha-ketoglutarate decarboxylase (NCBI) compare
b1335 ogt +0.2 1.2 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b0785 moaE +0.2 1.3 molybdopterin synthase, large subunit (NCBI) compare
b1515 lsrD +0.2 0.9 AI2 transporter (NCBI) compare
b4527 b1354 +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b4494 arpB +0.2 0.7 no description compare
b1346 ydaQ +0.2 0.6 orf, hypothetical protein (VIMSS) compare
b1130 phoP +0.2 0.5 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b2959 yggL +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b3206 npr +0.2 2.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4618 tisB +0.2 1.5 lexA-regulated toxic peptide (RefSeq) compare
b2398 yfeC +0.2 1.6 orf, hypothetical protein (VIMSS) compare
b4277 yjgZ +0.2 1.8 KpLE2 phage-like element; predicted protein (NCBI) compare
b1536 ydeI +0.2 0.4 hypothetical protein (NCBI) compare
b2051 wcaH +0.2 0.9 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2824 ygdB +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b4698 mgrR +0.2 1.2 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b1007 b1007 +0.2 0.8 orf, hypothetical protein (VIMSS) compare
b3613 yibP +0.2 0.7 putative membrane protein (VIMSS) compare
b4443 gcvB +0.2 0.1 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b2518 ndk +0.2 1.0 nucleoside diphosphate kinase (NCBI) compare
b1040 csgD +0.2 0.5 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b1387 maoC +0.2 1.2 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b2738 ygbL +0.2 1.1 hypothetical protein (NCBI) compare
b1382 ynbE +0.2 0.6 predicted lipoprotein (NCBI) compare
b2500 purN +0.2 1.7 phosphoribosylglycinamide formyltransferase (NCBI) compare
b4381 deoC +0.2 1.6 deoxyribose-phosphate aldolase (NCBI) compare
b4157 yjeN +0.2 1.3 hypothetical protein (NCBI) compare
b2618 b2618 +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b2579 yfiD +0.2 0.7 pyruvate formate lyase subunit (NCBI) compare
b0296 ykgM +0.2 1.4 50S ribosomal protein L31 (NCBI) compare
b2506 yfgI +0.2 0.9 hypothetical protein (NCBI) compare
b1573 ydfC +0.2 0.4 Qin prophage; predicted protein (RefSeq) compare
b4520 ymgF +0.2 0.6 hypothetical protein (NCBI) compare
b1750 ydjX +0.2 1.5 orf, hypothetical protein (VIMSS) compare
b1341 b1341 +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b2036 glf +0.2 1.8 UDP-galactopyranose mutase, FAD/NAD(P)-binding (NCBI) compare
b1751 ydjY +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b1113 ycfS +0.2 1.5 hypothetical protein (NCBI) compare
b1618 uidR +0.2 1.2 DNA-binding transcriptional repressor (NCBI) compare
b1699 ydiS +0.2 0.7 predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI) compare
b1689 b1689 +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b4234 yjgA +0.2 0.9 hypothetical protein (NCBI) compare
b2388 glk +0.2 1.6 glucokinase (NCBI) compare
b3006 exbB +0.2 1.7 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1723 pfkB +0.2 1.3 6-phosphofructokinase II; suppressor of pfkA (VIMSS) compare
b4656 yjhY +0.2 1.1 no description compare
b0746 valZ +0.2 0.9 tRNA-Val (NCBI) compare
b1376 ynaF +0.2 1.4 putative filament protein (VIMSS) compare
b4415 hokE +0.2 1.4 toxic polypeptide, small (NCBI) compare
b2202 napC +0.2 1.1 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b2314 dedD +0.2 1.3 putative lipoprotein (VIMSS) compare
b3361 fic +0.2 1.4 stationary-phase protein, cell division (NCBI) compare
b1158 pin +0.2 1.5 e14 prophage; site-specific DNA recombinase (NCBI) compare
b4108 phnA +0.2 1.6 hypothetical protein (NCBI) compare
b1035 ycdY +0.2 1.4 hypothetical protein (NCBI) compare
b2264 menD +0.2 1.6 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1600 mdtJ +0.2 0.5 multidrug efflux system transporter (NCBI) compare
b1977 asnT +0.2 0.9 tRNA-Asn (NCBI) compare
b1858 znuC +0.2 1.4 high-affinity zinc transporter ATPase (NCBI) compare
b1760 ynjH +0.2 1.2 hypothetical protein (NCBI) compare
b2814 metZ +0.2 0.6 tRNA-Met (NCBI) compare
b1086 rluC +0.2 1.3 23S rRNA pseudouridylate synthase (NCBI) compare
b0456 ybaA +0.2 0.9 hypothetical protein (NCBI) compare
b1785 yeaI +0.2 0.8 predicted diguanylate cyclase (NCBI) compare
b1038 csgF +0.2 0.8 predicted transport protein (NCBI) compare
b2044 wcaL +0.2 0.7 predicted glycosyl transferase (NCBI) compare
b4594 ymgJ +0.2 0.8 hypothetical protein (RefSeq) compare
b4633 xisD +0.2 1.2 no description compare
b2195 ccmG +0.2 0.7 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b1841 yobA +0.2 0.7 hypothetical protein (NCBI) compare
b1734 chbF +0.2 1.4 cryptic phospho-beta-glucosidase, NAD(P)-binding (NCBI) compare
b1409 ynbB +0.2 0.2 predicted CDP-diglyceride synthase (NCBI) compare
b2250 b2250 +0.2 1.2 orf, hypothetical protein (VIMSS) compare
b0210 yafE +0.2 1.3 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b1144 ymfJ +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b2248 yfaX +0.2 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2622 intA +0.2 1.6 CP4-57 prophage; integrase (NCBI) compare
b4480 hdfR +0.2 1.3 transcriptional regulator HdfR (NCBI) compare
b2859 ygeO +0.2 0.7 hypothetical protein (RefSeq) compare
b0961 yccF +0.2 1.2 conserved inner membrane protein (NCBI) compare
b2572 rseA +0.2 1.5 anti-sigma factor (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms