Experiment set41IT018 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time4hr

200 most detrimental genes:

  gene name fitness t score description  
b1361 ydaW +1.9 1.8 orf, hypothetical protein (VIMSS) compare
b2934 cmtB +1.4 1.2 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2846 yqeH +1.4 1.4 orf, hypothetical protein (VIMSS) compare
b1332 ynaJ +1.2 1.3 predicted inner membrane protein (NCBI) compare
b1936 intG +0.9 1.6 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4676 yoaK +0.9 1.0 expressed protein (RefSeq) compare
b0730 mngR +0.8 1.8 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b4538 yoeF +0.8 2.7 hypothetical protein (RefSeq) compare
b3120 yhaB +0.8 2.3 orf, hypothetical protein (VIMSS) compare
b4431 rprA +0.8 3.0 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1195 ymgE +0.8 2.7 predicted inner membrane protein (NCBI) compare
b1724 ydiZ +0.7 1.6 hypothetical protein (NCBI) compare
b1348 lar +0.7 2.4 Rac prophage; restriction alleviation protein (NCBI) compare
b0798 ybiA +0.6 2.9 hypothetical protein (NCBI) compare
b1164 ycgZ +0.6 1.7 hypothetical protein (NCBI) compare
b1160 elbA +0.6 0.9 hypothetical protein (NCBI) compare
b0542 renD +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b4480 hdfR +0.6 4.3 transcriptional regulator HdfR (NCBI) compare
b4509 ylcG +0.5 2.7 DLP12 prophage; predicted protein (NCBI) compare
b1831 proQ +0.5 2.2 putative solute/DNA competence effector (NCBI) compare
b4571 wbbL +0.5 2.8 no description compare
b1165 ymgA +0.5 0.3 hypothetical protein (NCBI) compare
b1070 flgN +0.5 1.5 export chaperone for FlgK and FlgL (NCBI) compare
b1753 ynjA +0.5 2.2 hypothetical protein (NCBI) compare
b4642 yoeD +0.5 1.5 no description compare
b0946 ycbW +0.5 3.0 orf, hypothetical protein (VIMSS) compare
b0802 ybiJ +0.5 1.9 hypothetical protein (NCBI) compare
b1506 yneL +0.5 1.1 predicted transcriptional regulator (NCBI) compare
b1408 b1408 +0.5 3.0 probable enzyme (VIMSS) compare
b4596 yciZ +0.5 1.4 hypothetical protein (RefSeq) compare
b0803 ybiI +0.5 2.7 hypothetical protein (NCBI) compare
b1476 fdnI +0.5 0.8 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b3860 dsbA +0.5 4.2 periplasmic protein disulfide isomerase I (NCBI) compare
b1742 b1742 +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b1841 yobA +0.4 1.6 hypothetical protein (NCBI) compare
b1179 ycgL +0.4 2.0 conserved protein (NCBI) compare
b0630 lipB +0.4 0.7 protein of lipoate biosynthesis (VIMSS) compare
b0683 fur +0.4 1.1 ferric uptake regulator (NCBI) compare
b3037 ygiB +0.4 2.6 conserved outer membrane protein (RefSeq) compare
b1085 b1085 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b2209 eco +0.4 0.8 ecotin precursor (NCBI) compare
b1434 ydcN +0.4 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b2051 wcaH +0.4 0.7 GDP-mannose mannosyl hydrolase (VIMSS) compare
b1185 dsbB +0.4 1.7 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1442 ydcU +0.4 2.3 predicted spermidine/putrescine transporter subunit (NCBI) compare
b1948 fliP +0.4 3.0 flagellar biosynthesis protein P (NCBI) compare
b1709 btuD +0.4 0.7 vitamin B12-transporter ATPase (NCBI) compare
b0714 nei +0.4 2.8 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b1357 ydaS +0.4 0.8 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1823 cspC +0.4 1.5 stress protein, member of the CspA-family (NCBI) compare
b1520 b1520 +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b2616 recN +0.4 3.1 recombination and repair protein (NCBI) compare
b4527 b1354 +0.4 2.5 orf, hypothetical protein (VIMSS) compare
b1038 csgF +0.4 1.7 predicted transport protein (NCBI) compare
b4045 yjbJ +0.4 1.6 predicted stress response protein (NCBI) compare
b1792 yeaO +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b2587 kgtP +0.4 2.3 alpha-ketoglutarate transporter (NCBI) compare
b0247 ykfG +0.4 1.1 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2399 yfeD +0.4 2.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0212 gloB +0.4 1.4 predicted hydroxyacylglutathione hydrolase (NCBI) compare
b1429 tehA +0.4 0.9 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b2532 yfhQ +0.4 2.3 predicted methyltransferase (NCBI) compare
b2261 menC +0.4 2.4 O-succinylbenzoate synthase (NCBI) compare
b4613 dinQ +0.4 1.6 Damage inducible, function unknown (RefSeq) compare
b1353 sieB +0.3 2.4 phage superinfection exclusion protein (VIMSS) compare
b2071 yegJ +0.3 1.0 hypothetical protein (NCBI) compare
b4410 ecnA +0.3 1.0 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b1387 maoC +0.3 2.2 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1103 ycfF +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b0388 aroL +0.3 2.4 shikimate kinase II (NCBI) compare
b4042 dgkA +0.3 0.2 diacylglycerol kinase (NCBI) compare
b1415 aldA +0.3 2.3 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b1567 intK +0.3 1.2 Qin prophage; predicted protein (RefSeq) compare
b0183 rnhB +0.3 2.2 ribonuclease HII (NCBI) compare
b1407 ydbD +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b1811 yoaH +0.3 1.9 hypothetical protein (NCBI) compare
b1958 yedI +0.3 1.9 conserved inner membrane protein (NCBI) compare
b1453 ansP +0.3 1.8 L-asparagine permease (VIMSS) compare
b2250 b2250 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2897 ygfY +0.3 1.8 hypothetical protein (NCBI) compare
b3627 rfaI +0.3 2.7 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b1954 dsrA +0.3 1.3 regulatory antisense RNA (NCBI) compare
b1930 yedF +0.3 2.3 hypothetical protein (NCBI) compare
b2398 yfeC +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b3199 yrbK +0.3 0.8 hypothetical protein (NCBI) compare
b1790 yeaM +0.3 1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b2577 yfiE +0.3 2.2 putative transcriptional regulator LYSR-type (VIMSS) compare
b4603 rseX +0.3 0.4 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b0218 yafU +0.3 1.2 predicted inner membrane protein (RefSeq) compare
b1789 yeaL +0.3 1.6 conserved inner membrane protein (NCBI) compare
b2279 nuoK +0.3 0.7 NADH dehydrogenase subunit K (NCBI) compare
b2368 emrK +0.3 2.4 EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component (NCBI) compare
b3446 yrhB +0.3 2.1 hypothetical protein (NCBI) compare
b4492 ydbA +0.3 2.5 no description compare
b2291 yfbR +0.3 1.4 hypothetical protein (NCBI) compare
b3027 ygiZ +0.3 0.2 conserved inner membrane protein (NCBI) compare
b0998 torD +0.3 0.7 chaperone protein TorD (NCBI) compare
b1768 ydjB +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b0596 entA +0.3 2.1 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI) compare
b1478 adhP +0.3 1.2 alcohol dehydrogenase (VIMSS) compare
b2481 hyfA +0.3 1.6 hydrogenase 4 Fe-S subunit (VIMSS) compare
b1686 ydiI +0.3 0.8 hypothetical protein (NCBI) compare
b1919 yedO +0.3 0.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b0641 rlpB +0.3 1.4 minor lipoprotein (NCBI) compare
b3100 yqjK +0.3 2.0 hypothetical protein (NCBI) compare
b1013 ycdC +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1672 ydhW +0.3 1.4 hypothetical protein (NCBI) compare
b1342 ydaN +0.3 1.9 zinc transporter (NCBI) compare
b2202 napC +0.3 2.0 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b1233 ychJ +0.3 1.7 hypothetical protein (NCBI) compare
b0548 ninE +0.3 1.8 DLP12 prophage; conserved protein (NCBI) compare
b0192 nlpE +0.3 2.0 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b0722 sdhD +0.3 1.7 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b3236 mdh +0.3 2.4 malate dehydrogenase (NCBI) compare
b2682 ygaZ +0.3 1.7 predicted transporter (NCBI) compare
b4640 yoeG +0.3 0.9 no description compare
b1410 ynbC +0.3 2.0 predicted hydrolase (NCBI) compare
b0942 ycbU +0.3 1.6 predicted fimbrial-like adhesin protein (NCBI) compare
b2275 yfbP +0.3 1.9 TPR-like repeats-containing protein (NCBI) compare
b0804 ybiX +0.3 1.4 putative enzyme (VIMSS) compare
b1763 topB +0.3 1.9 DNA topoisomerase III (NCBI) compare
b1395 paaH +0.3 2.3 3-hydroxybutyryl-CoA dehydrogenase (NCBI) compare
b1045 ymdB +0.3 1.8 hypothetical protein (NCBI) compare
b1053 mdtG +0.3 1.8 predicted drug efflux system (NCBI) compare
b2708 gutQ +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b3206 npr +0.3 2.0 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b4460 araH +0.3 1.3 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b4389 radA +0.3 2.3 predicted repair protein (NCBI) compare
b2736 ygbJ +0.3 1.4 predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b4546 ypeB +0.3 0.6 hypothetical protein (NCBI) compare
b1530 marR +0.3 1.1 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1427 rimL +0.3 1.1 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1168 b1168 +0.3 1.4 putative proteases (VIMSS) compare
b4104 phnE +0.3 1.4 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b2419 yfeK +0.3 1.0 hypothetical protein (NCBI) compare
b4594 ymgJ +0.3 1.0 hypothetical protein (RefSeq) compare
b0329 yahO +0.3 1.4 hypothetical protein (NCBI) compare
b3623 rfaK +0.3 0.9 lipopolysaccharide core biosynthesis (NCBI) compare
b0394 yajF +0.3 2.0 possible NAGC-like transcriptional regulator (VIMSS) compare
b1365 ynaK +0.3 1.9 Rac prophage; conserved protein (NCBI) compare
b1487 ddpA +0.3 1.3 D-ala-D-a la transporter subunit (NCBI) compare
b2893 dsbC +0.3 1.5 protein disulfide isomerase II (NCBI) compare
b1044 ymdA +0.3 1.5 hypothetical protein (NCBI) compare
b0976 hyaE +0.3 1.6 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b1422 ydcI +0.2 1.8 putative transcriptional regulator LYSR-type (VIMSS) compare
b0864 artP +0.2 1.4 arginine transporter subunit (NCBI) compare
b0209 yafD +0.2 1.6 hypothetical protein (NCBI) compare
b0393 rdgC +0.2 1.9 recombination associated protein (NCBI) compare
b2195 ccmG +0.2 0.7 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b4579 yaiX +0.2 1.7 no description compare
b0351 mhpF +0.2 1.8 acetaldehyde dehydrogenase (NCBI) compare
b4157 yjeN +0.2 1.4 hypothetical protein (NCBI) compare
b3021 ygiT +0.2 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b3248 yhdE +0.2 1.7 Maf-like protein (NCBI) compare
b1144 ymfJ +0.2 0.9 orf, hypothetical protein (VIMSS) compare
b1372 b1372 +0.2 1.8 putative membrane protein (VIMSS) compare
b1615 uidC +0.2 1.3 membrane-associated protein (VIMSS) compare
b3624 rfaZ +0.2 0.9 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0836 yliH +0.2 1.2 hypothetical protein (NCBI) compare
b0880 cspD +0.2 1.0 cold shock protein homolog (NCBI) compare
b2377 yfdY +0.2 1.0 predicted inner membrane protein (NCBI) compare
b3009 yghB +0.2 1.6 conserved inner membrane protein (NCBI) compare
b0407 yajC +0.2 0.8 preprotein translocase subunit YajC (NCBI) compare
b1384 feaR +0.2 1.6 DNA-binding transcriptional dual regulator (NCBI) compare
b1710 btuE +0.2 1.6 predicted glutathione peroxidase (NCBI) compare
b3686 ibpB +0.2 1.9 heat shock chaperone (NCBI) compare
b0296 ykgM +0.2 1.4 50S ribosomal protein L31 (NCBI) compare
b1081 flgJ +0.2 1.8 flagellar biosynthesis protein FlgJ (NCBI) compare
b1848 yebG +0.2 0.7 conserved protein regulated by LexA (NCBI) compare
b3764 yifE +0.2 1.5 hypothetical protein (NCBI) compare
b2356 yfdM +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b1347 ydaC +0.2 0.2 Rac prophage; predicted protein (NCBI) compare
b1172 ymgG +0.2 1.5 conserved protein, UPF0757 family (RefSeq) compare
b3006 exbB +0.2 1.9 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b0851 nfsA +0.2 1.2 nitroreductase A, NADPH-dependent, FMN-dependent (NCBI) compare
b1669 ydhT +0.2 0.8 hypothetical protein (NCBI) compare
b0228 yafM +0.2 1.4 hypothetical protein (NCBI) compare
b4387 ytjB +0.2 1.8 hypothetical protein (NCBI) compare
b0101 yacG +0.2 1.2 zinc-binding protein (NCBI) compare
b1502 ydeQ +0.2 0.7 predicted fimbrial-like adhesin protein (NCBI) compare
b1446 ydcY +0.2 0.9 hypothetical protein (NCBI) compare
b4513 kdpF +0.2 0.6 potassium ion accessory transporter subunit (NCBI) compare
b2408 yfeN +0.2 1.8 conserved outer membrane protein (NCBI) compare
b2009 sbmC +0.2 1.4 DNA gyrase inhibitor (NCBI) compare
b3017 sufI +0.2 2.0 repressor protein for FtsI (NCBI) compare
b0636 ybeA +0.2 1.6 hypothetical protein (NCBI) compare
b2998 yghW +0.2 1.2 hypothetical protein (NCBI) compare
b0920 ycbC +0.2 1.0 conserved inner membrane protein (NCBI) compare
b0933 ssuB +0.2 1.1 alkanesulfonate transporter subunit (NCBI) compare
b2189 proL +0.2 0.7 tRNA-Pro (NCBI) compare
b2579 yfiD +0.2 0.7 pyruvate formate lyase subunit (NCBI) compare
b4325 yjiC +0.2 1.1 orf, hypothetical protein (VIMSS) compare
b0846 ybjK +0.2 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1248 b1248 +0.2 0.8 orf, hypothetical protein (VIMSS) compare
b3838 tatB +0.2 1.6 sec-independent translocase (NCBI) compare
b2818 argA +0.2 1.9 N-acetylglutamate synthase (NCBI) compare
b0245 ykfI +0.2 1.4 CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b0521 ybcF +0.2 1.4 predicted carbamate kinase (NCBI) compare
b1917 yecC +0.2 1.4 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2698 recX +0.2 0.9 RecA regulator RecX (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms