Experiment set41IT015 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time1-2hr

200 most detrimental genes:

  gene name fitness t score description  
b2934 cmtB +1.6 1.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b4699 fnrS +1.3 2.1 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3979 thrT +1.2 0.5 tRNA-Thr (NCBI) compare
b3027 ygiZ +1.0 0.6 conserved inner membrane protein (NCBI) compare
b1165 ymgA +0.9 0.5 hypothetical protein (NCBI) compare
b4596 yciZ +0.8 2.1 hypothetical protein (RefSeq) compare
b0630 lipB +0.8 1.4 protein of lipoate biosynthesis (VIMSS) compare
b1576 ydfD +0.8 0.4 Qin prophage; predicted protein (NCBI) compare
b1545 pinQ +0.8 0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1351 racC +0.8 0.6 Rac prophage; predicted protein (NCBI) compare
b4538 yoeF +0.8 2.3 hypothetical protein (RefSeq) compare
b1205 ychH +0.8 1.5 predicted inner membrane protein (NCBI) compare
b0542 renD +0.8 2.6 orf, hypothetical protein (VIMSS) compare
b1499 ydeO +0.8 0.4 predicted DNA-binding transcriptional acfivator (NCBI) compare
b4505 insX +0.8 1.5 predicted IS protein (RefSeq) compare
b1257 yciE +0.7 0.4 hypothetical protein (NCBI) compare
b1409 ynbB +0.7 0.7 predicted CDP-diglyceride synthase (NCBI) compare
b0114 aceE +0.7 1.7 pyruvate dehydrogenase subunit E1 (NCBI) compare
b4497 yeeL +0.7 1.2 no description compare
b1506 yneL +0.7 1.5 predicted transcriptional regulator (NCBI) compare
b4503 yafF +0.7 1.3 no description compare
b1951 rcsA +0.7 1.4 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b3120 yhaB +0.7 2.1 orf, hypothetical protein (VIMSS) compare
b1167 ymgC +0.7 0.3 hypothetical protein (NCBI) compare
b1363 trkG +0.7 0.4 Rac prophage; potassium transporter subunit (NCBI) compare
b1497 b1497 +0.7 0.4 putative enzyme (VIMSS) compare
b1823 cspC +0.7 2.3 stress protein, member of the CspA-family (NCBI) compare
b0730 mngR +0.7 1.3 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1348 lar +0.6 1.8 Rac prophage; restriction alleviation protein (NCBI) compare
b1161 ycgX +0.6 1.0 hypothetical protein (NCBI) compare
b1762 b1762 +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b1505 ydeT +0.6 1.0 hypothetical protein (RefSeq) compare
b4494 arpB +0.6 1.3 no description compare
b1573 ydfC +0.6 1.1 Qin prophage; predicted protein (RefSeq) compare
b1058 yceO +0.6 0.3 hypothetical protein (NCBI) compare
b4533 ynfO +0.5 1.6 Qin prophage; predicted protein (NCBI) compare
b1961 dcm +0.5 1.1 DNA cytosine methylase (NCBI) compare
b1407 ydbD +0.5 3.2 orf, hypothetical protein (VIMSS) compare
b1176 minC +0.5 1.3 septum formation inhibitor (NCBI) compare
b4640 yoeG +0.5 1.5 no description compare
b1536 ydeI +0.5 0.6 hypothetical protein (NCBI) compare
b1038 csgF +0.5 2.0 predicted transport protein (NCBI) compare
b0310 ykgH +0.5 1.1 predicted inner membrane protein (NCBI) compare
b0407 yajC +0.5 1.1 preprotein translocase subunit YajC (NCBI) compare
b1001 yccE +0.5 0.2 hypothetical protein (NCBI) compare
b2859 ygeO +0.5 1.3 hypothetical protein (RefSeq) compare
b1936 intG +0.5 0.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b4642 yoeD +0.5 1.0 no description compare
b1542 ydfI +0.5 1.6 predicted mannonate dehydrogenase (NCBI) compare
b2055 wcaE +0.5 0.2 predicted glycosyl transferase (NCBI) compare
b1130 phoP +0.5 0.7 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b0714 nei +0.5 2.9 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b1182 hlyE +0.5 0.3 hemolysin E (VIMSS) compare
b2356 yfdM +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b4570 lomR +0.5 1.6 no description compare
b2792 yqcC +0.5 1.4 hypothetical protein (NCBI) compare
b1347 ydaC +0.4 0.2 Rac prophage; predicted protein (NCBI) compare
b2959 yggL +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b4152 frdC +0.4 2.8 fumarate reductase subunit C (NCBI) compare
b1679 sufE +0.4 1.5 cysteine desufuration protein SufE (NCBI) compare
b1025 ycdT +0.4 1.0 predicted diguanylate cyclase (NCBI) compare
b1690 ydiM +0.4 1.0 predicted transporter (NCBI) compare
b1940 fliH +0.4 2.4 flagellar biosynthesis; export of flagellar proteins? (VIMSS) compare
b4631 ykgQ +0.4 1.6 no description compare
b4551 yheV +0.4 1.3 hypothetical protein (NCBI) compare
b1339 abgR +0.4 2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2110 yehC +0.4 0.9 predicted periplasmic pilin chaperone (NCBI) compare
b4590 ybfK +0.4 0.6 hypothetical protein (RefSeq) compare
b1368 ynaA +0.4 1.5 Rac prophage; predicted tail protein (RefSeq) compare
b0713 ybgL +0.4 2.3 hypothetical protein (NCBI) compare
b1138 ymfE +0.4 0.9 e14 prophage; predicted inner membrane protein (NCBI) compare
b4618 tisB +0.4 2.2 lexA-regulated toxic peptide (RefSeq) compare
b1976 b1976 +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1168 b1168 +0.4 1.8 putative proteases (VIMSS) compare
b4431 rprA +0.4 1.2 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b1029 ycdU +0.4 2.4 predicted inner membrane protein (NCBI) compare
b0998 torD +0.4 0.7 chaperone protein TorD (NCBI) compare
b0247 ykfG +0.4 1.0 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1900 araG +0.4 2.0 fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2023 hisH +0.4 2.3 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b1129 phoQ +0.4 0.7 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b1332 ynaJ +0.4 0.3 predicted inner membrane protein (NCBI) compare
b2897 ygfY +0.4 1.8 hypothetical protein (NCBI) compare
b2058 wcaB +0.4 0.8 predicted acyl transferase (NCBI) compare
b1258 yciF +0.4 0.4 hypothetical protein (NCBI) compare
b1399 paaX +0.4 1.6 DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI) compare
b1353 sieB +0.4 2.3 phage superinfection exclusion protein (VIMSS) compare
b4042 dgkA +0.4 0.2 diacylglycerol kinase (NCBI) compare
b4515 ybgT +0.4 1.1 hypothetical protein (NCBI) compare
b2809 b2809 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1330 ynaI +0.4 1.7 conserved inner membrane protein (NCBI) compare
b3623 rfaK +0.4 1.3 lipopolysaccharide core biosynthesis (NCBI) compare
b1480 sra +0.4 0.5 30S ribosomal subunit protein S22 (NCBI) compare
b3068 ygjF +0.4 2.3 G/U mismatch-specific DNA glycosylase (NCBI) compare
b4655 ythA +0.3 1.2 expressed protein (RefSeq) compare
b1305 pspB +0.3 0.9 phage shock protein B (NCBI) compare
b0933 ssuB +0.3 1.7 alkanesulfonate transporter subunit (NCBI) compare
b0926 ycbK +0.3 2.3 hypothetical protein (NCBI) compare
b1760 ynjH +0.3 2.1 hypothetical protein (NCBI) compare
b1408 b1408 +0.3 1.9 probable enzyme (VIMSS) compare
b1954 dsrA +0.3 1.3 regulatory antisense RNA (NCBI) compare
b2175 spr +0.3 1.4 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b3100 yqjK +0.3 1.8 hypothetical protein (NCBI) compare
b1511 ydeV +0.3 2.0 putative kinase (VIMSS) compare
b0850 ybjC +0.3 1.1 predicted inner membrane protein (NCBI) compare
b4464 ygfQ +0.3 2.6 predicted transporter (NCBI) compare
b1686 ydiI +0.3 0.6 hypothetical protein (NCBI) compare
b1742 b1742 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1530 marR +0.3 1.4 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b0802 ybiJ +0.3 1.2 hypothetical protein (NCBI) compare
b1558 cspF +0.3 1.0 Qin prophage; cold shock protein (NCBI) compare
b1342 ydaN +0.3 1.9 zinc transporter (NCBI) compare
b4646 yrdE +0.3 1.8 no description compare
b4480 hdfR +0.3 2.1 transcriptional regulator HdfR (NCBI) compare
b1304 pspA +0.3 1.5 regulatory protein for phage-shock-protein operon (NCBI) compare
b1831 proQ +0.3 1.2 putative solute/DNA competence effector (NCBI) compare
b1028 ymdE +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b0754 aroG +0.3 2.2 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, phenylalanine repressible (NCBI) compare
b1495 yddB +0.3 2.4 predicted porin protein (NCBI) compare
b1838 pphA +0.3 1.7 protein phosphatase 1 modulates phosphoproteins, signals protein misfolding (VIMSS) compare
b4151 frdD +0.3 1.5 fumarate reductase subunit D (NCBI) compare
b2212 alkB +0.3 1.9 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b3169 nusA +0.3 0.8 transcription elongation factor NusA (NCBI) compare
b1572 ydfB +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b2768 ygcP +0.3 1.3 predicted anti-terminator regulatory protein (NCBI) compare
b2399 yfeD +0.3 1.6 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1134 ymfB +0.3 1.2 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) compare
b0546 ybcM +0.3 2.0 DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0803 ybiI +0.3 1.5 hypothetical protein (NCBI) compare
b0108 ppdD +0.3 2.3 predicted major pilin subunit (NCBI) compare
b4656 yjhY +0.3 1.9 no description compare
b1551 b1551 +0.3 0.2 orf, hypothetical protein (VIMSS) compare
b3037 ygiB +0.3 1.5 conserved outer membrane protein (RefSeq) compare
b0878 b0878 +0.3 1.5 putative membrane protein (VIMSS) compare
b3875 ompL +0.3 1.5 predicted outer membrane porin L (NCBI) compare
b2195 ccmG +0.3 1.1 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b0737 tolQ +0.3 0.7 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b2284 nuoF +0.3 1.8 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b1930 yedF +0.3 1.4 hypothetical protein (NCBI) compare
b0549 ybcO +0.3 1.1 DLP12 prophage; predicted protein (NCBI) compare
b1410 ynbC +0.3 1.6 predicted hydrolase (NCBI) compare
b2674 nrdI +0.3 1.7 hypothetical protein (NCBI) compare
b1519 tam +0.3 1.7 trans-aconitate 2-methyltransferase (NCBI) compare
b2250 b2250 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b0295 ykgL +0.3 2.2 hypothetical protein (NCBI) compare
b0339 cynT +0.3 1.3 carbonic anhydrase (NCBI) compare
b2435 amiA +0.3 2.0 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b0942 ycbU +0.3 1.7 predicted fimbrial-like adhesin protein (NCBI) compare
b1732 katE +0.3 1.7 hydroperoxidase HPII(III) (catalase) (NCBI) compare
b3129 sohA +0.3 1.9 predicted regulator (NCBI) compare
b1341 b1341 +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b4301 sgcE +0.3 1.8 ribulose-phosphate 3-epimerase (NCBI) compare
b1465 narV +0.3 0.7 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1387 maoC +0.3 1.8 fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI) compare
b1153 ymfQ +0.3 1.0 e14 prophage; conserved protein (NCBI) compare
b2362 yfdS +0.3 1.2 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b4363 yjjB +0.3 1.6 conserved inner membrane protein (RefSeq) compare
b2187 yejL +0.3 0.6 hypothetical protein (NCBI) compare
b2230 yfaA +0.3 2.1 orf, hypothetical protein (VIMSS) compare
b0974 hyaC +0.3 1.0 hydrogenase 1, b-type cytochrome subunit (NCBI) compare
b0705 ybfL +0.3 0.2 putative receptor protein (VIMSS) compare
b1143 ymfI +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b0535 fimZ +0.3 1.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b4493 gapC +0.3 1.1 no description compare
b2804 fucU +0.3 1.5 L-fucose mutarotase (NCBI) compare
b0323 yahI +0.3 1.7 predicted carbamate kinase-like protein (NCBI) compare
b1281 pyrF +0.3 1.2 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0632 dacA +0.3 2.2 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1085 b1085 +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b1453 ansP +0.3 1.6 L-asparagine permease (VIMSS) compare
b4153 frdB +0.3 2.2 fumarate reductase (anaerobic), Fe-S subunit (NCBI) compare
b1698 ydiR +0.3 1.5 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b1693 aroD +0.3 1.4 3-dehydroquinate dehydratase (NCBI) compare
b0696 kdpC +0.3 0.9 potassium-transporting ATPase subunit C (NCBI) compare
b0820 ybiT +0.3 2.0 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2367 emrY +0.3 1.8 predicted multidrug efflux system (NCBI) compare
b1948 fliP +0.3 1.4 flagellar biosynthesis protein P (NCBI) compare
b1121 ycfZ +0.3 0.6 predicted inner membrane protein (NCBI) compare
b2258 b2258 +0.3 0.6 putative transport/receptor protein (VIMSS) compare
b1691 ydiN +0.3 1.0 predicted transporter (RefSeq) compare
b4487 yjdP +0.3 1.9 hypothetical protein (NCBI) compare
b2915 yqfE +0.3 1.2 hypothetical protein (RefSeq) compare
b0102 yacF +0.3 1.3 hypothetical protein (NCBI) compare
b1758 b1758 +0.3 0.5 putative cytochrome oxidase (VIMSS) compare
b0245 ykfI +0.3 1.7 CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b1335 ogt +0.3 1.2 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b1485 ddpC +0.3 0.9 D-ala-D-ala transporter subunit (NCBI) compare
b0821 ybiU +0.3 2.1 hypothetical protein (NCBI) compare
b1993 cobU +0.3 1.4 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI) compare
b1070 flgN +0.3 0.6 export chaperone for FlgK and FlgL (NCBI) compare
b1792 yeaO +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b3545 proK +0.3 1.0 tRNA-Pro (NCBI) compare
b4492 ydbA +0.3 2.1 no description compare
b4675 yoaJ +0.3 1.0 expressed protein (RefSeq) compare
b2579 yfiD +0.3 0.7 pyruvate formate lyase subunit (NCBI) compare
b0521 ybcF +0.3 1.5 predicted carbamate kinase (NCBI) compare
b4408 csrB +0.3 0.9 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b4313 fimE +0.3 2.1 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b2002 yeeS +0.3 1.6 CP4-44 prophage; predicted DNA repair protein (NCBI) compare
b2141 yohJ +0.3 1.3 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms