Experiment set41IT005 for Escherichia coli BW25113

Compare to:

LB + ciprofloxacin Time3hr

200 most detrimental genes:

  gene name fitness t score description  
b4704 arrS +0.9 0.5 Antisense sRNA ArrS, function unknown (NCBI) compare
b0730 mngR +0.9 1.9 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1936 intG +0.9 1.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1361 ydaW +0.8 0.7 orf, hypothetical protein (VIMSS) compare
b4596 yciZ +0.8 2.0 hypothetical protein (RefSeq) compare
b0998 torD +0.7 1.7 chaperone protein TorD (NCBI) compare
b1176 minC +0.6 1.8 septum formation inhibitor (NCBI) compare
b1476 fdnI +0.6 1.1 formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI) compare
b2934 cmtB +0.6 0.5 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b3120 yhaB +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b1195 ymgE +0.6 1.7 predicted inner membrane protein (NCBI) compare
b0798 ybiA +0.5 2.2 hypothetical protein (NCBI) compare
b1730 ydjO +0.5 1.1 predicted protein (RefSeq) compare
b1557 cspB +0.5 1.6 Qin prophage; cold shock protein (NCBI) compare
b1742 b1742 +0.5 2.3 orf, hypothetical protein (VIMSS) compare
b0630 lipB +0.5 0.8 protein of lipoate biosynthesis (VIMSS) compare
b1753 ynjA +0.5 2.3 hypothetical protein (NCBI) compare
b3790 rffC +0.5 3.2 TDP-fucosamine acetyltransferase (RefSeq) compare
b0114 aceE +0.5 1.1 pyruvate dehydrogenase subunit E1 (NCBI) compare
b0218 yafU +0.5 2.1 predicted inner membrane protein (RefSeq) compare
b4538 yoeF +0.5 1.3 hypothetical protein (RefSeq) compare
b1882 cheY +0.5 0.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1765 ydjA +0.5 0.8 predicted oxidoreductase (NCBI) compare
b4406 yaeP +0.5 1.8 hypothetical protein (NCBI) compare
b4642 yoeD +0.4 1.3 no description compare
b4045 yjbJ +0.4 1.9 predicted stress response protein (NCBI) compare
b1357 ydaS +0.4 1.8 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b4042 dgkA +0.4 0.3 diacylglycerol kinase (NCBI) compare
b2532 yfhQ +0.4 2.7 predicted methyltransferase (NCBI) compare
b4592 yccB +0.4 1.2 hypothetical protein (RefSeq) compare
b4603 rseX +0.4 0.6 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3250 mreC +0.4 1.3 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b3791 rffA +0.4 3.1 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0542 renD +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b1831 proQ +0.4 1.6 putative solute/DNA competence effector (NCBI) compare
b1179 ycgL +0.4 2.0 conserved protein (NCBI) compare
b3623 rfaK +0.4 1.5 lipopolysaccharide core biosynthesis (NCBI) compare
b2051 wcaH +0.4 0.8 GDP-mannose mannosyl hydrolase (VIMSS) compare
b2287 nuoB +0.4 2.2 NADH dehydrogenase subunit B (NCBI) compare
b1506 yneL +0.4 0.6 predicted transcriptional regulator (NCBI) compare
b0802 ybiJ +0.4 1.9 hypothetical protein (NCBI) compare
b0381 ddlA +0.4 2.9 D-alanylalanine synthetase (NCBI) compare
b2846 yqeH +0.4 0.4 orf, hypothetical protein (VIMSS) compare
b1930 yedF +0.4 2.5 hypothetical protein (NCBI) compare
b0641 rlpB +0.4 1.9 minor lipoprotein (NCBI) compare
b4418 sraB +0.4 0.8 unknown RNA (NCBI) compare
b2264 menD +0.4 2.9 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1448 yncA +0.4 1.0 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2064 asmA +0.4 2.7 predicted assembly protein (NCBI) compare
b1113 ycfS +0.4 2.2 hypothetical protein (NCBI) compare
b1482 osmC +0.3 1.1 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b0193 yaeF +0.3 2.6 orf, hypothetical protein (VIMSS) compare
b1949 fliQ +0.3 1.0 flagellar biosynthesis protein Q (NCBI) compare
b1205 ychH +0.3 1.3 predicted inner membrane protein (NCBI) compare
b1007 b1007 +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1919 yedO +0.3 1.3 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b2809 b2809 +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b1515 lsrD +0.3 1.3 AI2 transporter (NCBI) compare
b1530 marR +0.3 1.6 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b3863 polA +0.3 0.5 DNA polymerase I (NCBI) compare
b2208 napF +0.3 2.0 ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI) compare
b1185 dsbB +0.3 1.9 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4410 ecnA +0.3 0.9 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b3786 rffE +0.3 2.8 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b0209 yafD +0.3 2.4 hypothetical protein (NCBI) compare
b3787 rffD +0.3 2.7 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0843 ybjH +0.3 0.8 hypothetical protein (NCBI) compare
b1575 dicB +0.3 0.9 Qin prophage; cell division inhibition protein (NCBI) compare
b2915 yqfE +0.3 1.6 hypothetical protein (RefSeq) compare
b1586 b1586 +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b1792 yeaO +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b0247 ykfG +0.3 0.9 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1727 yniC +0.3 1.3 predicted hydrolase (NCBI) compare
b3624 rfaZ +0.3 1.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2188 yejM +0.3 2.4 predicted hydrolase, inner membrane (NCBI) compare
b4692 oweE +0.3 1.4 no description compare
b1103 ycfF +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1038 csgF +0.3 1.4 predicted transport protein (NCBI) compare
b1153 ymfQ +0.3 1.0 e14 prophage; conserved protein (NCBI) compare
b2399 yfeD +0.3 1.8 predicted DNA-binding transcriptional regulator (RefSeq) compare
b1261 trpB +0.3 1.5 tryptophan synthase subunit beta (NCBI) compare
b1520 b1520 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b1175 minD +0.3 1.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b4513 kdpF +0.3 0.7 potassium ion accessory transporter subunit (NCBI) compare
b4609 ryfD +0.3 1.6 Novel sRNA, function unknown (RefSeq) compare
b4546 ypeB +0.3 0.6 hypothetical protein (NCBI) compare
b1553 ydfP +0.3 0.6 Qin prophage; conserved protein (NCBI) compare
b0696 kdpC +0.3 0.9 potassium-transporting ATPase subunit C (NCBI) compare
b1977 asnT +0.3 0.9 tRNA-Asn (NCBI) compare
b0880 cspD +0.3 1.0 cold shock protein homolog (NCBI) compare
b0245 ykfI +0.3 2.3 CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b0294 ykgK +0.3 1.5 predicted regulator (NCBI) compare
b0183 rnhB +0.3 1.6 ribonuclease HII (NCBI) compare
b2776 ygcE +0.3 2.1 predicted kinase (NCBI) compare
b1260 trpA +0.3 2.0 tryptophan synthase subunit alpha (NCBI) compare
b4481 rffT +0.3 1.9 4-alpha-L-fucosyltransferase (NCBI) compare
b3037 ygiB +0.3 1.7 conserved outer membrane protein (RefSeq) compare
b1351 racC +0.3 0.2 Rac prophage; predicted protein (NCBI) compare
b1487 ddpA +0.3 1.8 D-ala-D-a la transporter subunit (NCBI) compare
b0726 sucA +0.3 1.3 alpha-ketoglutarate decarboxylase (NCBI) compare
b4646 yrdE +0.3 1.1 no description compare
b1689 b1689 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b3100 yqjK +0.3 1.5 hypothetical protein (NCBI) compare
b2250 b2250 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b4367 fhuF +0.3 2.1 ferric iron reductase involved in ferric hydroximate transport (NCBI) compare
b3836 b3836 +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b1573 ydfC +0.3 0.6 Qin prophage; predicted protein (RefSeq) compare
b3962 udhA +0.3 1.9 putative oxidoreductase (VIMSS) compare
b3236 mdh +0.3 2.0 malate dehydrogenase (NCBI) compare
b1768 ydjB +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b2408 yfeN +0.2 1.9 conserved outer membrane protein (NCBI) compare
b4543 ypaA +0.2 1.4 predicted protein (NCBI) compare
b4579 yaiX +0.2 1.8 no description compare
b1380 ldhA +0.2 1.2 D-lactate dehydrogenase (NCBI) compare
b3392 yrfA +0.2 2.0 orf, hypothetical protein (VIMSS) compare
b1729 ydjN +0.2 1.1 predicted transporter (NCBI) compare
b4363 yjjB +0.2 1.4 conserved inner membrane protein (RefSeq) compare
b0804 ybiX +0.2 1.9 putative enzyme (VIMSS) compare
b2435 amiA +0.2 1.7 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b2010 dacD +0.2 1.8 penicillin binding protein 6b (VIMSS) compare
b2490 b2490 +0.2 1.6 putative protein processing element (VIMSS) compare
b4552 yrhC +0.2 0.6 no description compare
b0920 ycbC +0.2 1.2 conserved inner membrane protein (NCBI) compare
b2261 menC +0.2 1.8 O-succinylbenzoate synthase (NCBI) compare
b0192 nlpE +0.2 1.7 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b4458 oxyS +0.2 0.4 global regulatory RNA (NCBI) compare
b1566 flxA +0.2 1.3 Qin prophage; predicted protein (NCBI) compare
b1811 yoaH +0.2 1.1 hypothetical protein (NCBI) compare
b1849 purT +0.2 1.6 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1408 b1408 +0.2 1.4 probable enzyme (VIMSS) compare
b1963 b1963 +0.2 1.4 orf, hypothetical protein (VIMSS) compare
b1085 b1085 +0.2 0.8 orf, hypothetical protein (VIMSS) compare
b4541 yehK +0.2 1.3 hypothetical protein (NCBI) compare
b4588 ylcH +0.2 1.4 hypothetical protein, DLP12 prophage (RefSeq) compare
b2202 napC +0.2 1.2 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b4246 pyrL +0.2 1.2 pyrBI operon leader peptide (NCBI) compare
b4280 yjhC +0.2 1.8 putative dehydrogenase (VIMSS) compare
b1948 fliP +0.2 1.7 flagellar biosynthesis protein P (NCBI) compare
b0329 yahO +0.2 1.5 hypothetical protein (NCBI) compare
b3627 rfaI +0.2 1.9 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b2481 hyfA +0.2 1.6 hydrogenase 4 Fe-S subunit (VIMSS) compare
b2916 argP +0.2 1.7 chromosome replication initiation inhibitor protein (NCBI) compare
b0310 ykgH +0.2 0.5 predicted inner membrane protein (NCBI) compare
b1097 yceG +0.2 1.4 predicted aminodeoxychorismate lyase (NCBI) compare
b2213 ada +0.2 1.4 fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI) compare
b2096 gatY +0.2 1.2 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2388 glk +0.2 1.5 glucokinase (NCBI) compare
b3483 yhhH +0.2 0.1 orf, hypothetical protein (VIMSS) compare
b1105 ycfM +0.2 1.0 predicted outer membrane lipoprotein (NCBI) compare
b2193 narP +0.2 1.7 DNA-binding response regulator in two-component regulatory system with NarQ or NarX (NCBI) compare
b1355 b1355 +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b4277 yjgZ +0.2 1.9 KpLE2 phage-like element; predicted protein (NCBI) compare
b4595 yciY +0.2 1.0 hypothetical protein (RefSeq) compare
b1251 yciI +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b3794 rffM +0.2 1.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b4157 yjeN +0.2 1.1 hypothetical protein (NCBI) compare
b1418 cybB +0.2 0.8 cytochrome b(561) (VIMSS) compare
b1446 ydcY +0.2 0.9 hypothetical protein (NCBI) compare
b4444 rygA +0.2 1.2 unknown RNA (NCBI) compare
b1556 b1556 +0.2 0.7 orf, hypothetical protein (VIMSS) compare
b3068 ygjF +0.2 1.3 G/U mismatch-specific DNA glycosylase (NCBI) compare
b2689 yqaA +0.2 1.2 conserved inner membrane protein (NCBI) compare
b0934 ycbM +0.2 1.2 putative transport system permease protein (VIMSS) compare
b4687 shoB +0.2 1.6 toxic membrane protein (RefSeq) compare
b1710 btuE +0.2 1.5 predicted glutathione peroxidase (NCBI) compare
b2486 hyfF +0.2 1.6 NADH dehydrogenase subunit N (NCBI) compare
b1847 yebF +0.2 0.6 predicted protein (RefSeq) compare
b4039 ubiC +0.2 1.6 chorismate lyase (VIMSS) compare
b1306 pspC +0.2 0.6 DNA-binding transcriptional activator (NCBI) compare
b0846 ybjK +0.2 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b1415 aldA +0.2 1.6 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b4331 yjiI +0.2 1.8 orf, hypothetical protein (VIMSS) compare
b0101 yacG +0.2 1.0 zinc-binding protein (NCBI) compare
b1822 rrmA +0.2 0.9 23S rRNA m1G745 methyltransferase (NCBI) compare
b2818 argA +0.2 1.6 N-acetylglutamate synthase (NCBI) compare
b1421 trg +0.2 1.2 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b3860 dsbA +0.2 1.9 periplasmic protein disulfide isomerase I (NCBI) compare
b4388 serB +0.2 1.7 3-phosphoserine phosphatase (NCBI) compare
b3725 pstB +0.2 0.1 phosphate transporter subunit (NCBI) compare
b1945 fliM +0.2 1.6 flagellar motor switch protein M (NCBI) compare
b2187 yejL +0.2 0.9 hypothetical protein (NCBI) compare
b1686 ydiI +0.2 0.5 hypothetical protein (NCBI) compare
b1348 lar +0.2 0.6 Rac prophage; restriction alleviation protein (NCBI) compare
b2794 yqcD +0.2 1.5 hypothetical protein (NCBI) compare
b2209 eco +0.2 0.5 ecotin precursor (NCBI) compare
b4032 malG +0.2 1.6 maltose transporter subunit (NCBI) compare
b2009 sbmC +0.2 1.2 DNA gyrase inhibitor (NCBI) compare
b2587 kgtP +0.2 1.5 alpha-ketoglutarate transporter (NCBI) compare
b2352 yfdI +0.2 0.3 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b3792 wzxE +0.2 1.6 O-antigen translocase (NCBI) compare
b4000 hupA +0.2 1.2 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1623 add +0.2 1.2 adenosine deaminase (NCBI) compare
b1077 flgF +0.2 1.6 flagellar component of cell-proximal portion of basal-body rod (NCBI) compare
b4640 yoeG +0.2 0.7 no description compare
b3784 rfe +0.2 1.8 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b0632 dacA +0.2 1.6 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1962 yedJ +0.2 1.6 predicted phosphohydrolase (NCBI) compare
b1607 ydgC +0.2 0.7 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b2275 yfbP +0.2 1.5 TPR-like repeats-containing protein (NCBI) compare
b0388 aroL +0.2 1.3 shikimate kinase II (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Ciprofloxacin Hydrochloride across organisms