Experiment set41IT003 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time1-2hr

200 most important genes:

  gene name fitness t score description  
b0462 acrB -5.3 -27.1 multidrug efflux system protein (NCBI) compare
b0463 acrA -4.9 -18.2 multidrug efflux system (NCBI) compare
b4237 nrdG -4.8 -7.9 anaerobic ribonucleotide reductase activating protein (NCBI) compare
b4238 nrdD -4.5 -12.0 anaerobic ribonucleoside triphosphate reductase (NCBI) compare
b4053 alr -4.4 -17.6 alanine racemase (NCBI) compare
b4041 plsB -3.7 -5.7 glycerol-3-phosphate acyltransferase (VIMSS) compare
b0386 proC -2.9 -4.8 pyrroline-5-carboxylate reductase (NCBI) compare
b2620 smpB -2.8 -1.9 SsrA-binding protein (NCBI) compare
b4201 priB -2.3 -5.1 primosomal replication protein N (NCBI) compare
b0912 ihfB -2.2 -2.6 integration host factor subunit beta (NCBI) compare
b0949 uup -2.1 -10.0 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4699 fnrS -2.0 -1.3 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3035 tolC -2.0 -1.9 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0243 proA -1.9 -11.3 gamma-glutamyl phosphate reductase (NCBI) compare
b1290 sapF -1.7 -2.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1677 lpp -1.7 -1.8 murein lipoprotein (NCBI) compare
b0242 proB -1.5 -6.4 gamma-glutamyl kinase (NCBI) compare
b2912 ygfA -1.5 -2.9 putative ligase (VIMSS) compare
b4535 yniD -1.4 -1.9 predicted protein (RefSeq) compare
b1126 potA -1.4 -6.9 putrescine/spermidine ABC transporter ATPase protein (NCBI) compare
b1361 ydaW -1.3 -0.6 orf, hypothetical protein (VIMSS) compare
b1575 dicB -1.2 -1.6 Qin prophage; cell division inhibition protein (NCBI) compare
b1123 potD -1.2 -4.1 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b3863 polA -1.2 -1.3 DNA polymerase I (NCBI) compare
b4520 ymgF -1.2 -1.5 hypothetical protein (NCBI) compare
b1648 b1648 -1.1 -2.1 orf, hypothetical protein (VIMSS) compare
b1124 potC -1.1 -5.2 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1855 lpxM -1.0 -3.0 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b1125 potB -1.0 -3.6 spermidine/putrescine ABC transporter membrane protein (NCBI) compare
b1850 eda -1.0 -1.8 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b0232 yafN -1.0 -1.1 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b2820 recB -1.0 -0.6 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b2114 metG -1.0 -2.8 methionyl-tRNA synthetase (NCBI) compare
b1830 prc -1.0 -3.8 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b1334 fnr -0.9 -1.1 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b4647 mokA -0.9 -1.3 no description compare
b0688 pgm -0.9 -2.3 phosphoglucomutase (NCBI) compare
b2659 b2659 -0.9 -3.5 orf, hypothetical protein (VIMSS) compare
b3229 sspA -0.9 -2.2 stringent starvation protein A (NCBI) compare
b0902 pflA -0.9 -4.3 pyruvate formate lyase activating enzyme 1 (NCBI) compare
b0200 gmhB -0.8 -1.1 hypothetical protein (NCBI) compare
b0903 pflB -0.8 -5.9 pyruvate formate lyase I (NCBI) compare
b3082 ygjM -0.8 -1.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b3052 rfaE -0.8 -3.7 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2814 metZ -0.8 -1.8 tRNA-Met (NCBI) compare
b4066 yjcF -0.7 -0.6 hypothetical protein (NCBI) compare
b1292 sapC -0.7 -1.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1992 cobS -0.7 -1.4 cobalamin synthase (NCBI) compare
b0167 glnD -0.7 -2.9 PII uridylyl-transferase (NCBI) compare
b1088 yceD -0.7 -2.5 hypothetical protein (NCBI) compare
b1685 ydiH -0.7 -1.3 predicted protein (RefSeq) compare
b1199 dhaL -0.7 -3.1 dihydroxyacetone kinase, C-terminal domain (NCBI) compare
b1147 ymfL -0.7 -1.7 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b3935 priA -0.7 -2.2 primosome assembly protein PriA (NCBI) compare
b4593 ymgI -0.7 -0.6 hypothetical protein (RefSeq) compare
b1607 ydgC -0.7 -0.8 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b1853 yebK -0.6 -1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1187 fadR -0.6 -2.0 fatty acid metabolism regulator (NCBI) compare
b1591 b1591 -0.6 -1.1 putative oxidoreductase component (VIMSS) compare
b2576 srmB -0.6 -2.0 ATP-dependent RNA helicase (NCBI) compare
b1860 ruvB -0.6 -0.6 Holliday junction DNA helicase B (NCBI) compare
b1164 ycgZ -0.6 -1.1 hypothetical protein (NCBI) compare
b4416 rybA -0.6 -1.3 unknown RNA (NCBI) compare
b1593 b1593 -0.6 -2.0 orf, hypothetical protein (VIMSS) compare
b3781 trxA -0.6 -2.3 thioredoxin 1 (VIMSS) compare
b0990 cspG -0.6 -0.6 DNA-binding transcriptional regulator (NCBI) compare
b3636 rpmG -0.6 -1.9 50S ribosomal protein L33 (NCBI) compare
b0234 yafP -0.6 -1.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b2822 recC -0.6 -3.2 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b3609 secB -0.6 -2.2 export protein SecB (NCBI) compare
b4685 yrbN -0.6 -2.2 expressed protein (RefSeq) compare
b1765 ydjA -0.6 -0.6 predicted oxidoreductase (NCBI) compare
b4163 glyV -0.6 -1.7 tRNA-Gly (NCBI) compare
b2642 yfjW -0.6 -0.9 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1847 yebF -0.6 -1.1 predicted protein (RefSeq) compare
b3930 menA -0.5 -4.9 1,4-dihydroxy-2-naphthoate octaprenyltransferase (NCBI) compare
b1293 sapB -0.5 -0.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1067 yceH -0.5 -2.7 hypothetical protein (NCBI) compare
b1821 b1821 -0.5 -2.4 orf, hypothetical protein (VIMSS) compare
b0685 ybfE -0.5 -1.4 orf, hypothetical protein (VIMSS) compare
b3839 tatC -0.5 -3.1 TatABCE protein translocation system subunit (NCBI) compare
b3290 trkA -0.5 -0.6 potassium transporter peripheral membrane component (NCBI) compare
b2343 b2343 -0.5 -1.2 orf, hypothetical protein (VIMSS) compare
b1835 yebU -0.5 -2.1 putative nucleolar proteins (VIMSS) compare
b2699 recA -0.5 -0.3 recombinase A (NCBI) compare
b1921 fliZ -0.5 -2.0 orf, hypothetical protein (VIMSS) compare
b3183 obgE -0.5 -0.8 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1622 malY -0.5 -2.1 bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI) compare
b2509 xseA -0.5 -4.0 exodeoxyribonuclease VII large subunit (NCBI) compare
b1692 ydiB -0.5 -1.8 quinate/shikimate 5-dehydrogenase, NAD(P)-binding (NCBI) compare
b3500 gor -0.5 -1.4 glutathione reductase (NCBI) compare
b2646 ypjF -0.5 -2.0 CP4-57 prophage; toxin of the YpjF-YfjZ toxin-antitoxin system (NCBI) compare
b2007 yeeX -0.5 -1.2 conserved protein (RefSeq) compare
b2262 menB -0.5 -2.5 naphthoate synthase (NCBI) compare
b3868 glnG -0.5 -3.5 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b3652 recG -0.5 -4.1 ATP-dependent DNA helicase (NCBI) compare
b4215 ytfI -0.5 -1.2 hypothetical protein (NCBI) compare
b1196 ycgY -0.5 -1.7 hypothetical protein (NCBI) compare
b1659 ydhB -0.5 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b0249 ykfF -0.5 -1.6 CP4-6 prophage; predicted protein (NCBI) compare
b0948 ycbY -0.5 -2.7 predicted methyltransferase (NCBI) compare
b2024 hisA -0.5 -1.9 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b4545 ypdJ -0.5 -1.6 no description compare
b1263 trpD -0.5 -1.3 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b2288 nuoA -0.5 -1.7 NADH dehydrogenase I chain A (VIMSS) compare
b1533 ydeD -0.5 -0.9 orf, hypothetical protein (VIMSS) compare
b2279 nuoK -0.5 -1.2 NADH dehydrogenase subunit K (NCBI) compare
b1602 pntB -0.5 -2.6 pyridine nucleotide transhydrogenase (NCBI) compare
b1754 b1754 -0.5 -1.6 orf, hypothetical protein (VIMSS) compare
b1480 sra -0.5 -0.6 30S ribosomal subunit protein S22 (NCBI) compare
b1221 narL -0.5 -2.6 DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI) compare
b0965 b0965 -0.5 -1.9 orf, hypothetical protein (VIMSS) compare
b2285 nuoE -0.5 -1.6 NADH dehydrogenase subunit E (NCBI) compare
b0222 lpcA -0.5 -1.3 phosphoheptose isomerase (NCBI) compare
b1493 gadB -0.5 -0.6 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b1628 rsxB -0.5 -1.8 electron transport complex protein RnfB (NCBI) compare
b1661 cfa -0.5 -2.2 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b1620 malI -0.5 -1.2 DNA-binding transcriptional repressor (NCBI) compare
b2726 hypA -0.5 -2.2 protein involved in nickel insertion into hydrogenases 3 (NCBI) compare
b0145 dksA -0.5 -1.2 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b2329 aroC -0.5 -2.5 chorismate synthase (NCBI) compare
b4375 prfC -0.4 -1.5 peptide chain release factor 3 (NCBI) compare
b1482 osmC -0.4 -1.0 osmotically inducible, stress-inducible membrane protein (NCBI) compare
b3261 fis -0.4 -0.5 DNA-binding protein Fis (NCBI) compare
b3493 pitA -0.4 -3.0 phosphate transporter, low-affinity (NCBI) compare
b1594 dgsA -0.4 -1.9 DNA-binding transcriptional repressor (NCBI) compare
b1589 ynfG -0.4 -1.7 oxidoreductase, Fe-S subunit (NCBI) compare
b1842 holE -0.4 -1.3 DNA polymerase III, theta subunit (NCBI) compare
b0549 ybcO -0.4 -1.0 DLP12 prophage; predicted protein (NCBI) compare
b1192 ldcA -0.4 -0.8 L,D-carboxypeptidase A (NCBI) compare
b1346 ydaQ -0.4 -0.8 orf, hypothetical protein (VIMSS) compare
b2450 yffS -0.4 -1.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b4523 yciX -0.4 -2.2 hypothetical protein (RefSeq) compare
b0118 acnB -0.4 -0.3 aconitate hydratase (NCBI) compare
b1817 manX -0.4 -2.7 fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI) compare
b0088 murD -0.4 -1.5 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b1804 rnd -0.4 -1.6 ribonuclease D (NCBI) compare
b1084 rne -0.4 -2.3 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b1617 uidA -0.4 -2.2 beta-D-glucuronidase (NCBI) compare
b1259 yciG -0.4 -1.0 orf, hypothetical protein (VIMSS) compare
b0683 fur -0.4 -0.6 ferric uptake regulator (NCBI) compare
b0806 ybiM -0.4 -1.6 orf, hypothetical protein (VIMSS) compare
b1383 ydbL -0.4 -1.5 orf, hypothetical protein (VIMSS) compare
b1531 marA -0.4 -1.4 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b4493 gapC -0.4 -1.0 no description compare
b2652 ileY -0.4 -0.4 tRNA-Ile (NCBI) compare
b2528 iscA -0.4 -1.2 iron-sulfur cluster assembly protein (NCBI) compare
b1518 lsrG -0.4 -0.6 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b2704 srlB -0.4 -1.2 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b4676 yoaK -0.4 -0.2 expressed protein (RefSeq) compare
b1291 sapD -0.4 -0.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1148 ymfM -0.4 -1.6 e14 prophage; predicted protein (NCBI) compare
b1217 chaB -0.4 -0.7 cation transport regulator (NCBI) compare
b2754 ygbF -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b4171 miaA -0.4 -1.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0999 cbpM -0.4 -1.8 modulator of CbpA co-chaperone (NCBI) compare
b1269 rluB -0.4 -1.7 23S rRNA pseudouridylate synthase (NCBI) compare
b0882 clpA -0.4 -2.8 ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI) compare
b0920 ycbC -0.4 -2.0 conserved inner membrane protein (NCBI) compare
b1012 b1012 -0.4 -2.2 orf, hypothetical protein (VIMSS) compare
b0721 sdhC -0.4 -1.5 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b4570 lomR -0.4 -1.0 no description compare
b1429 tehA -0.4 -0.6 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1186 nhaB -0.4 -2.8 NhaB sodium/proton transporter (Katherine Huang) compare
b0053 surA -0.4 -2.4 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3164 pnp -0.4 -1.6 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b0379 yaiY -0.4 -2.2 predicted inner membrane protein (NCBI) compare
b0407 yajC -0.4 -0.5 preprotein translocase subunit YajC (NCBI) compare
b2110 yehC -0.4 -0.4 predicted periplasmic pilin chaperone (NCBI) compare
b4409 blr -0.4 -2.4 beta-lactam resistance membrane protein (NCBI) compare
b0471 ybaB -0.4 -1.7 hypothetical protein (NCBI) compare
b1171 b1171 -0.4 -0.9 orf, hypothetical protein (VIMSS) compare
b1151 ymfO -0.4 -1.6 e14 prophage; conserved protein (RefSeq) compare
b2580 ung -0.4 -2.2 uracil-DNA glycosylase (NCBI) compare
b1522 yneF -0.4 -1.3 predicted diguanylate cyclase (NCBI) compare
b1931 yedK -0.4 -1.5 hypothetical protein (NCBI) compare
b1336 ydaH -0.4 -2.0 putative pump protein (transport) (VIMSS) compare
b0881 clpS -0.4 -1.2 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b1592 b1592 -0.4 -2.4 putative chloride channel (VIMSS) compare
b2531 iscR -0.4 -0.8 DNA-binding transcriptional repressor (NCBI) compare
b0998 torD -0.4 -0.6 chaperone protein TorD (NCBI) compare
b0564 appY -0.4 -0.7 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b1223 narK -0.3 -1.3 nitrate/nitrite transporter (NCBI) compare
b0980 appA -0.3 -1.8 phosphoanhydride phosphorylase (NCBI) compare
b2281 nuoI -0.3 -1.3 NADH dehydrogenase subunit I (NCBI) compare
b2666 yqaE -0.3 -2.3 predicted membrane protein (NCBI) compare
b1889 motB -0.3 -1.8 flagellar motor protein MotB (NCBI) compare
b1844 exoX -0.3 -1.8 exodeoxyribonuclease X (NCBI) compare
b3838 tatB -0.3 -2.5 sec-independent translocase (NCBI) compare
b4589 ylcI -0.3 -1.5 hypothetical protein (RefSeq) compare
b0866 ybjQ -0.3 -0.7 hypothetical protein (NCBI) compare
b1708 nlpC -0.3 -1.7 predicted lipoprotein (NCBI) compare
b1953 b1953 -0.3 -1.5 orf, hypothetical protein (VIMSS) compare
b3210 arcB -0.3 -2.3 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b3813 uvrD -0.3 -3.0 DNA-dependent ATPase I and helicase II (NCBI) compare
b2817 b2817 -0.3 -2.3 putative amidase (VIMSS) compare
b1891 flhC -0.3 -1.6 DNA-binding transcriptional dual regulator with FlhD (NCBI) compare
b0560 nohB -0.3 -1.4 DLP12 prophage; DNA packaging protein (NCBI) compare
b3162 deaD -0.3 -1.6 inducible ATP-independent RNA helicase (VIMSS) compare
b1273 yciN -0.3 -0.7 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms