Experiment set41IT003 for Escherichia coli BW25113

Compare to:

LB + carbenicillin Time1-2hr

200 most detrimental genes:

  gene name fitness t score description  
b4538 yoeF +1.2 3.8 hypothetical protein (RefSeq) compare
b1936 intG +1.2 1.9 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1351 racC +1.1 0.9 Rac prophage; predicted protein (NCBI) compare
b1332 ynaJ +1.0 1.1 predicted inner membrane protein (NCBI) compare
b1576 ydfD +0.9 0.5 Qin prophage; predicted protein (NCBI) compare
b1409 ynbB +0.9 0.9 predicted CDP-diglyceride synthase (NCBI) compare
b1545 pinQ +0.9 0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b0630 lipB +0.9 1.5 protein of lipoate biosynthesis (VIMSS) compare
b1499 ydeO +0.9 0.4 predicted DNA-binding transcriptional acfivator (NCBI) compare
b1524 yneH +0.9 1.5 predicted glutaminase (NCBI) compare
b1363 trkG +0.9 0.6 Rac prophage; potassium transporter subunit (NCBI) compare
b4603 rseX +0.9 1.0 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1497 b1497 +0.8 0.5 putative enzyme (VIMSS) compare
b1257 yciE +0.8 0.4 hypothetical protein (NCBI) compare
b1160 elbA +0.8 1.0 hypothetical protein (NCBI) compare
b1040 csgD +0.7 1.4 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b1724 ydiZ +0.7 1.6 hypothetical protein (NCBI) compare
b1357 ydaS +0.7 2.4 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1167 ymgC +0.7 0.3 hypothetical protein (NCBI) compare
b1165 ymgA +0.7 0.3 hypothetical protein (NCBI) compare
b0617 citD +0.7 2.9 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1691 ydiN +0.7 2.7 predicted transporter (RefSeq) compare
b1753 ynjA +0.7 1.8 hypothetical protein (NCBI) compare
b0880 cspD +0.6 2.4 cold shock protein homolog (NCBI) compare
b0623 cspE +0.6 2.1 cold shock protein E (NCBI) compare
b1520 b1520 +0.6 2.2 orf, hypothetical protein (VIMSS) compare
b4640 yoeG +0.6 1.6 no description compare
b1865 nudB +0.6 1.3 dATP pyrophosphohydrolase (NCBI) compare
b1195 ymgE +0.6 1.8 predicted inner membrane protein (NCBI) compare
b1863 ruvC +0.6 0.9 Holliday junction resolvase (NCBI) compare
b1058 yceO +0.6 0.3 hypothetical protein (NCBI) compare
b4494 arpB +0.6 1.1 no description compare
b2071 yegJ +0.6 1.7 hypothetical protein (NCBI) compare
b4529 ydbJ +0.6 1.1 hypothetical protein (NCBI) compare
b2846 yqeH +0.6 0.5 orf, hypothetical protein (VIMSS) compare
b1690 ydiM +0.6 1.3 predicted transporter (NCBI) compare
b1506 yneL +0.5 0.9 predicted transcriptional regulator (NCBI) compare
b1347 ydaC +0.5 0.3 Rac prophage; predicted protein (NCBI) compare
b1001 yccE +0.5 0.3 hypothetical protein (NCBI) compare
b2055 wcaE +0.5 0.3 predicted glycosyl transferase (NCBI) compare
b1709 btuD +0.5 0.9 vitamin B12-transporter ATPase (NCBI) compare
b1882 cheY +0.5 0.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1182 hlyE +0.5 0.3 hemolysin E (VIMSS) compare
b4590 ybfK +0.5 0.7 hypothetical protein (RefSeq) compare
b1613 manA +0.5 1.4 mannose-6-phosphate isomerase (VIMSS) compare
b2058 wcaB +0.5 1.1 predicted acyl transferase (NCBI) compare
b1950 fliR +0.5 1.8 flagellar biosynthesis protein R (NCBI) compare
b2579 yfiD +0.5 1.7 pyruvate formate lyase subunit (NCBI) compare
b1669 ydhT +0.5 1.9 hypothetical protein (NCBI) compare
b0705 ybfL +0.5 0.4 putative receptor protein (VIMSS) compare
b1536 ydeI +0.5 0.4 hypothetical protein (NCBI) compare
b1505 ydeT +0.5 0.5 hypothetical protein (RefSeq) compare
b1138 ymfE +0.5 1.1 e14 prophage; predicted inner membrane protein (NCBI) compare
b1258 yciF +0.5 0.5 hypothetical protein (NCBI) compare
b3037 ygiB +0.5 3.1 conserved outer membrane protein (RefSeq) compare
b1362 rzpR +0.5 1.8 putative Rac prophage endopeptidase (VIMSS) compare
b1348 lar +0.5 1.1 Rac prophage; restriction alleviation protein (NCBI) compare
b4458 oxyS +0.5 0.8 global regulatory RNA (NCBI) compare
b1742 b1742 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b1551 b1551 +0.5 0.3 orf, hypothetical protein (VIMSS) compare
b1734 chbF +0.4 2.9 cryptic phospho-beta-glucosidase, NAD(P)-binding (NCBI) compare
b1085 b1085 +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b0101 yacG +0.4 2.2 zinc-binding protein (NCBI) compare
b1490 yddV +0.4 2.3 predicted diguanylate cyclase (RefSeq) compare
b0730 mngR +0.4 0.8 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1762 b1762 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1530 marR +0.4 1.7 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b1407 ydbD +0.4 2.3 orf, hypothetical protein (VIMSS) compare
b4631 ykgQ +0.4 1.3 no description compare
b1686 ydiI +0.4 0.9 hypothetical protein (NCBI) compare
b1733 chbG +0.4 1.2 hypothetical protein (NCBI) compare
b1503 ydeR +0.4 1.6 predicted fimbrial-like adhesin protein (NCBI) compare
b4551 yheV +0.4 2.3 hypothetical protein (NCBI) compare
b1839 yebY +0.4 1.4 hypothetical protein (NCBI) compare
b2859 ygeO +0.4 1.3 hypothetical protein (RefSeq) compare
b2853 ygeI +0.4 0.2 hypothetical protein (NCBI) compare
b1025 ycdT +0.4 0.8 predicted diguanylate cyclase (NCBI) compare
b0450 glnK +0.4 1.9 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b4592 yccB +0.4 1.5 hypothetical protein (RefSeq) compare
b0682 ybfN +0.4 2.7 predicted lipoprotein (NCBI) compare
b1984 asnW +0.4 0.8 tRNA-Asn (NCBI) compare
b2221 atoD +0.4 1.6 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b3624 rfaZ +0.4 1.6 lipopolysaccharide core biosynthesis protein (NCBI) compare
b4503 yafF +0.4 0.7 no description compare
b4585 sroB +0.4 0.9 Novel sRNA, function unknown (RefSeq) compare
b2934 cmtB +0.4 0.3 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b2532 yfhQ +0.4 2.4 predicted methyltransferase (NCBI) compare
b0946 ycbW +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1461 ydcE +0.4 1.4 4-oxalocrotonate tautomerase (NCBI) compare
b2051 wcaH +0.4 1.2 GDP-mannose mannosyl hydrolase (VIMSS) compare
b1431 ydcL +0.4 1.6 predicted lipoprotein (NCBI) compare
b1144 ymfJ +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b1330 ynaI +0.4 1.7 conserved inner membrane protein (NCBI) compare
b1954 dsrA +0.4 1.4 regulatory antisense RNA (NCBI) compare
b1729 ydjN +0.4 1.2 predicted transporter (NCBI) compare
b3443 yrhA +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b4702 mgtL +0.4 1.3 regulatory leader peptide for mgtA (NCBI) compare
b0641 rlpB +0.4 1.1 minor lipoprotein (NCBI) compare
b3120 yhaB +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b2804 fucU +0.4 1.1 L-fucose mutarotase (NCBI) compare
b4497 yeeL +0.4 0.4 no description compare
b1502 ydeQ +0.3 0.8 predicted fimbrial-like adhesin protein (NCBI) compare
b1563 relE +0.3 0.8 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b4648 ysaC +0.3 1.9 no description compare
b4642 yoeD +0.3 0.7 no description compare
b1415 aldA +0.3 2.1 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b4045 yjbJ +0.3 1.5 predicted stress response protein (NCBI) compare
b4653 yicT +0.3 1.5 no description compare
b2399 yfeD +0.3 1.8 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2486 hyfF +0.3 2.4 NADH dehydrogenase subunit N (NCBI) compare
b2506 yfgI +0.3 1.2 hypothetical protein (NCBI) compare
b0926 ycbK +0.3 2.5 hypothetical protein (NCBI) compare
b1130 phoP +0.3 0.4 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b1789 yeaL +0.3 1.2 conserved inner membrane protein (NCBI) compare
b1566 flxA +0.3 1.3 Qin prophage; predicted protein (NCBI) compare
b0406 tgt +0.3 1.8 queuine tRNA-ribosyltransferase (NCBI) compare
b1877 yecT +0.3 0.9 orf, hypothetical protein (VIMSS) compare
b1337 abgB +0.3 1.7 predicted peptidase, aminobenzoyl-glutamate utilization protein (NCBI) compare
b0456 ybaA +0.3 1.2 hypothetical protein (NCBI) compare
b2074 b2074 +0.3 2.1 putative membrane protein (VIMSS) compare
b4695 ykgT +0.3 0.9 no description compare
b1712 ihfA +0.3 0.2 integration host factor subunit alpha (NCBI) compare
b1656 sodB +0.3 1.1 superoxide dismutase, Fe (NCBI) compare
b1902 yecI +0.3 1.8 predicted ferritin-like protein (NCBI) compare
b3021 ygiT +0.3 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b4543 ypaA +0.3 1.7 predicted protein (NCBI) compare
b1344 ydaO +0.3 0.7 predicted C32 tRNA thiolase (NCBI) compare
b1730 ydjO +0.3 0.4 predicted protein (RefSeq) compare
b2356 yfdM +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b2363 yfdT +0.3 1.0 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b2264 menD +0.3 2.4 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI) compare
b1919 yedO +0.3 1.8 putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS) compare
b1579 intQ +0.3 0.9 Qin prophage; predicted defective integrase (RefSeq) compare
b3169 nusA +0.3 0.9 transcription elongation factor NusA (NCBI) compare
b1172 ymgG +0.3 1.7 conserved protein, UPF0757 family (RefSeq) compare
b1152 ymfP +0.3 1.4 orf, hypothetical protein (VIMSS) compare
b2935 tktA +0.3 0.7 transketolase 1, thiamin-binding (NCBI) compare
b1462 b1462 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b1514 lsrC +0.3 1.1 AI2 transporter (NCBI) compare
b0611 rna +0.3 1.7 ribonuclease I (NCBI) compare
b1790 yeaM +0.3 0.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2998 yghW +0.3 1.7 hypothetical protein (NCBI) compare
b1785 yeaI +0.3 0.9 predicted diguanylate cyclase (NCBI) compare
b0439 lon +0.3 1.3 DNA-binding ATP-dependent protease La (NCBI) compare
b0388 aroL +0.3 2.0 shikimate kinase II (NCBI) compare
b2368 emrK +0.3 2.3 EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component (NCBI) compare
b1129 phoQ +0.3 0.4 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b4513 kdpF +0.3 0.9 potassium ion accessory transporter subunit (NCBI) compare
b2587 kgtP +0.3 1.7 alpha-ketoglutarate transporter (NCBI) compare
b4277 yjgZ +0.3 1.8 KpLE2 phage-like element; predicted protein (NCBI) compare
b3973 birA +0.3 1.3 biotin--protein ligase (NCBI) compare
b4609 ryfD +0.3 1.6 Novel sRNA, function unknown (RefSeq) compare
b1548 nohA +0.3 0.6 Qin prophage; predicted packaging protein (RefSeq) compare
b0736 ybgC +0.3 1.0 predicted acyl-CoA thioesterase (NCBI) compare
b2293 yfbT +0.3 1.2 putative phosphatase (VIMSS) compare
b0843 ybjH +0.3 0.8 hypothetical protein (NCBI) compare
b1751 ydjY +0.3 1.1 orf, hypothetical protein (VIMSS) compare
b1045 ymdB +0.3 1.4 hypothetical protein (NCBI) compare
b2682 ygaZ +0.3 1.8 predicted transporter (NCBI) compare
b1325 ycjG +0.3 1.3 putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS) compare
b1792 yeaO +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b3153 yhbO +0.3 1.9 orf, hypothetical protein (VIMSS) compare
b2359 yfdP +0.3 1.5 CPS-53 (KpLE1) prophage; predicted protein (RefSeq) compare
b2698 recX +0.3 1.1 RecA regulator RecX (NCBI) compare
b0927 ycbL +0.3 1.0 predicted metal-binding enzyme (NCBI) compare
b0934 ycbM +0.3 1.1 putative transport system permease protein (VIMSS) compare
b4596 yciZ +0.3 0.5 hypothetical protein (RefSeq) compare
b4573 insZ +0.3 1.4 no description compare
b4522 yciX_1 +0.3 1.0 hypothetical protein (NCBI) compare
b1408 b1408 +0.3 1.4 probable enzyme (VIMSS) compare
b3027 ygiZ +0.3 0.1 conserved inner membrane protein (NCBI) compare
b2618 b2618 +0.3 1.3 orf, hypothetical protein (VIMSS) compare
b3074 ygjH +0.3 1.9 hypothetical protein (NCBI) compare
b1143 ymfI +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b1007 b1007 +0.3 0.7 orf, hypothetical protein (VIMSS) compare
b0247 ykfG +0.3 0.7 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0521 ybcF +0.3 1.5 predicted carbamate kinase (NCBI) compare
b4431 rprA +0.3 0.7 Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq) compare
b3908 sodA +0.3 1.8 superoxide dismutase, manganese (VIMSS) compare
b2582 trxC +0.3 1.5 thioredoxin 2 (NCBI) compare
b2510 b2510 +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b1581 rspA +0.2 0.9 predicted dehydratase (NCBI) compare
b1423 ydcJ +0.2 1.4 hypothetical protein (NCBI) compare
b0269 yagF +0.2 2.0 CP4-6 prophage; predicted dehydratase (NCBI) compare
b1139 lit +0.2 1.2 e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI) compare
b1550 b1550 +0.2 0.4 orf, hypothetical protein (VIMSS) compare
b2163 yeiL +0.2 1.5 putative transcriptional regulator (VIMSS) compare
b0193 yaeF +0.2 2.0 orf, hypothetical protein (VIMSS) compare
b0192 nlpE +0.2 1.7 lipoprotein involved with copper homeostasis and adhesion (NCBI) compare
b4627 ykfL +0.2 1.1 no description compare
b1610 tus +0.2 1.0 DNA replication terminus site-binding protein (NCBI) compare
b4687 shoB +0.2 1.6 toxic membrane protein (RefSeq) compare
b2083 yegZ +0.2 1.0 orf, hypothetical protein (VIMSS) compare
b2202 napC +0.2 1.1 nitrate reductase, cytochrome c-type, periplasmic (NCBI) compare
b2023 hisH +0.2 1.5 imidazole glycerol phosphate synthase subunit HisH (NCBI) compare
b3555 yiaG +0.2 1.6 predicted transcriptional regulator (NCBI) compare
b2876 b2876 +0.2 1.7 orf, hypothetical protein (VIMSS) compare
b2263 yfbB +0.2 1.0 predicted peptidase (NCBI) compare
b4152 frdC +0.2 1.6 fumarate reductase subunit C (NCBI) compare
b1418 cybB +0.2 0.5 cytochrome b(561) (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Carbenicillin disodium salt across organisms