Experiment set40IT088 for Escherichia coli BW25113

Compare to:

carbon source D-Mannose 0.5mM

200 most detrimental genes:

  gene name fitness t score description  
b2904 gcvH +3.3 8.2 glycine cleavage system protein H (NCBI) compare
b2903 gcvP +2.8 22.9 glycine dehydrogenase (NCBI) compare
b2905 gcvT +2.0 7.5 glycine cleavage system aminomethyltransferase T (NCBI) compare
b4592 yccB +1.8 3.0 hypothetical protein (RefSeq) compare
b1506 yneL +1.8 2.9 predicted transcriptional regulator (NCBI) compare
b0627 tatE +1.5 3.3 twin arginine translocase protein E (NCBI) compare
b2650 ypjC +1.5 0.6 hypothetical protein (RefSeq) compare
b1362 rzpR +1.5 2.1 putative Rac prophage endopeptidase (VIMSS) compare
b1108 ycfP +1.4 2.0 orf, hypothetical protein (VIMSS) compare
b4505 insX +1.4 1.5 predicted IS protein (RefSeq) compare
b3107 yhaL +1.4 2.0 orf, hypothetical protein (VIMSS) compare
b1332 ynaJ +1.4 0.9 predicted inner membrane protein (NCBI) compare
b3443 yrhA +1.4 2.5 orf, hypothetical protein (VIMSS) compare
b3181 greA +1.4 7.3 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b0688 pgm +1.4 3.2 phosphoglucomutase (NCBI) compare
b1594 dgsA +1.3 4.4 DNA-binding transcriptional repressor (NCBI) compare
b2168 fruK +1.3 1.4 1-phosphofructokinase (NCBI) compare
b4701 sokX +1.3 0.6 sok-related sRNA, function unknown (RefSeq) compare
b4699 fnrS +1.3 1.2 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1361 ydaW +1.3 1.0 orf, hypothetical protein (VIMSS) compare
b1583 ynfB +1.2 1.6 hypothetical protein (NCBI) compare
b1486 ddpB +1.2 2.8 D-ala-D-ala transporter subunit (NCBI) compare
b4594 ymgJ +1.2 1.7 hypothetical protein (RefSeq) compare
b2948 yqgE +1.1 2.9 orf, hypothetical protein (VIMSS) compare
b2490 b2490 +1.1 2.0 putative protein processing element (VIMSS) compare
b4203 rplI +1.1 4.5 50S ribosomal protein L9 (NCBI) compare
b4655 ythA +1.1 1.4 expressed protein (RefSeq) compare
b4568 ytjA +1.1 2.0 predicted protein (RefSeq) compare
b3163 nlpI +1.1 3.4 hypothetical protein (NCBI) compare
b0310 ykgH +1.1 1.9 predicted inner membrane protein (NCBI) compare
b4458 oxyS +1.1 1.2 global regulatory RNA (NCBI) compare
b4402 yjjY +1.1 0.6 hypothetical protein (NCBI) compare
b4653 yicT +1.1 2.4 no description compare
b2808 gcvA +1.1 3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b1660 ydhC +1.1 2.9 predicted transporter (NCBI) compare
b1271 yciK +1.1 2.1 short chain dehydrogenase (NCBI) compare
b1190 dadX +1.1 3.3 alanine racemase (NCBI) compare
b2012 yeeD +1.1 3.2 hypothetical protein (NCBI) compare
b1556 b1556 +1.1 1.5 orf, hypothetical protein (VIMSS) compare
b2947 gshB +1.1 3.2 glutathione synthetase (NCBI) compare
b4104 phnE +1.0 2.4 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b4144 yjeI +1.0 1.7 orf, hypothetical protein (VIMSS) compare
b3199 yrbK +1.0 1.4 hypothetical protein (NCBI) compare
b2765 ygcM +1.0 2.4 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) (NCBI) compare
b1418 cybB +1.0 2.0 cytochrome b(561) (VIMSS) compare
b4631 ykgQ +1.0 1.5 no description compare
b1814 sdaA +1.0 2.1 L-serine deaminase I (NCBI) compare
b2823 ppdC +1.0 2.5 hypothetical protein (NCBI) compare
b0802 ybiJ +1.0 2.0 hypothetical protein (NCBI) compare
b3836 b3836 +1.0 1.8 orf, hypothetical protein (VIMSS) compare
b4630 ykgP +1.0 2.3 no description compare
b2634 yfjR +1.0 2.1 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1360 ydaV +1.0 1.3 Rac prophage; predicted DNA replication protein (NCBI) compare
b1434 ydcN +0.9 0.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1575 dicB +0.9 0.8 Qin prophage; cell division inhibition protein (NCBI) compare
b2058 wcaB +0.9 1.0 predicted acyl transferase (NCBI) compare
b1454 yncG +0.9 2.4 predicted enzyme (NCBI) compare
b0605 ahpC +0.9 2.9 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1830 prc +0.9 3.8 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b3630 rfaP +0.9 5.9 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1095 fabF +0.9 4.8 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0504 ybbS +0.9 2.4 DNA-binding transcriptional activator of the allD operon (NCBI) compare
b1085 b1085 +0.9 2.0 orf, hypothetical protein (VIMSS) compare
b1861 ruvA +0.9 1.0 Holliday junction DNA helicase motor protein (NCBI) compare
b0247 ykfG +0.9 1.0 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b4373 rimI +0.9 2.0 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b2742 nlpD +0.9 4.2 predicted outer membrane lipoprotein (NCBI) compare
b3889 yiiE +0.9 2.3 predicted transcriptional regulator (RefSeq) compare
b0707 ybgA +0.8 1.9 hypothetical protein (NCBI) compare
b1465 narV +0.8 1.9 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b3194 yrbE +0.8 4.5 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b1217 chaB +0.8 1.4 cation transport regulator (NCBI) compare
b4694 yagP +0.8 1.6 no description compare
b4045 yjbJ +0.8 1.3 predicted stress response protein (NCBI) compare
b2829 ptsP +0.8 5.7 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b1618 uidR +0.8 1.3 DNA-binding transcriptional repressor (NCBI) compare
b1853 yebK +0.8 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b3150 yraP +0.8 3.1 hypothetical protein (NCBI) compare
b1518 lsrG +0.8 0.6 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1186 nhaB +0.8 3.8 NhaB sodium/proton transporter (Katherine Huang) compare
b0051 ksgA +0.8 2.5 dimethyladenosine transferase (NCBI) compare
b1380 ldhA +0.8 1.4 D-lactate dehydrogenase (NCBI) compare
b2209 eco +0.8 1.2 ecotin precursor (NCBI) compare
b1685 ydiH +0.8 1.1 predicted protein (RefSeq) compare
b0729 sucD +0.8 2.0 succinyl-CoA synthetase subunit alpha (NCBI) compare
b3973 birA +0.8 1.1 biotin--protein ligase (NCBI) compare
b1341 b1341 +0.8 3.6 orf, hypothetical protein (VIMSS) compare
b3842 rfaH +0.8 2.6 transcriptional activator RfaH (NCBI) compare
b2141 yohJ +0.8 1.5 hypothetical protein (NCBI) compare
b1106 ycfN +0.8 1.8 thiamin kinase (NCBI) compare
b3755 yieP +0.8 3.1 predicted transcriptional regulator (NCBI) compare
b0218 yafU +0.7 1.6 predicted inner membrane protein (RefSeq) compare
b0857 potI +0.7 1.7 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2688 gshA +0.7 3.1 glutamate--cysteine ligase (NCBI) compare
b1179 ycgL +0.7 1.5 conserved protein (NCBI) compare
b3999 yjaG +0.7 1.9 hypothetical protein (NCBI) compare
b0059 hepA +0.7 5.3 ATP-dependent helicase HepA (NCBI) compare
b4595 yciY +0.7 1.2 hypothetical protein (RefSeq) compare
b2110 yehC +0.7 1.2 predicted periplasmic pilin chaperone (NCBI) compare
b3934 cytR +0.7 5.1 DNA-binding transcriptional dual regulator (NCBI) compare
b3753 rbsR +0.7 4.6 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b0927 ycbL +0.7 2.0 predicted metal-binding enzyme (NCBI) compare
b4148 sugE +0.7 2.1 suppresses groEL, may be chaperone (VIMSS) compare
b2259 pmrD +0.7 0.7 polymyxin resistance protein B (VIMSS) compare
b3083 ygjN +0.7 1.8 hypothetical protein (NCBI) compare
b4100 phnH +0.7 1.3 carbon-phosphorus lyase complex subunit (NCBI) compare
b1630 rsxD +0.7 2.1 electron transport complex protein RnfD (NCBI) compare
b1778 yeaA +0.7 1.2 methionine sulfoxide reductase B (NCBI) compare
b4017 arpA +0.7 3.1 regulator of acetyl CoA synthetase (NCBI) compare
b4702 mgtL +0.7 0.7 regulatory leader peptide for mgtA (NCBI) compare
b1426 ydcH +0.7 0.9 orf, hypothetical protein (VIMSS) compare
b4646 yrdE +0.7 1.8 no description compare
b2262 menB +0.7 1.5 naphthoate synthase (NCBI) compare
b1514 lsrC +0.7 1.3 AI2 transporter (NCBI) compare
b3198 kdsC +0.7 1.5 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b2301 yfcF +0.7 2.2 predicted enzyme (NCBI) compare
b0728 sucC +0.7 2.9 succinyl-CoA synthetase subunit beta (NCBI) compare
b1348 lar +0.7 0.9 Rac prophage; restriction alleviation protein (NCBI) compare
b4232 fbp +0.7 4.0 fructose-1,6-bisphosphatase (NCBI) compare
b2804 fucU +0.7 1.2 L-fucose mutarotase (NCBI) compare
b1754 b1754 +0.7 1.2 orf, hypothetical protein (VIMSS) compare
b3631 rfaG +0.7 4.9 glucosyltransferase I (NCBI) compare
b3034 nudF +0.7 2.4 ADP-ribose pyrophosphatase (NCBI) compare
b2127 mlrA +0.7 2.2 DNA-binding transcriptional regulator (NCBI) compare
b2052 fcl +0.7 2.2 bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI) compare
b1918 yecS +0.7 1.2 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b2426 ucpA +0.7 1.2 putative oxidoreductase (VIMSS) compare
b1943 fliK +0.7 2.0 flagellar hook-length control protein (NCBI) compare
b3203 yhbH +0.6 1.3 predicted ribosome-associated, sigma 54 modulation protein (NCBI) compare
b2817 b2817 +0.6 2.9 putative amidase (VIMSS) compare
b1749 xthA +0.6 1.5 exonuclease III (NCBI) compare
b1191 b1191 +0.6 3.6 orf, hypothetical protein (VIMSS) compare
b3404 envZ +0.6 3.1 osmolarity sensor protein (NCBI) compare
b1651 gloA +0.6 1.9 glyoxalase I, Ni-dependent (NCBI) compare
b4025 pgi +0.6 3.0 glucose-6-phosphate isomerase (NCBI) compare
b2727 hypB +0.6 1.3 GTP hydrolase involved in nickel liganding into hydrogenases (NCBI) compare
b3590 selB +0.6 4.1 selenocysteinyl-tRNA-specific translation factor (NCBI) compare
b3929 rraA +0.6 3.0 ribonuclease activity regulator protein RraA (NCBI) compare
b3366 nirD +0.6 1.7 nitrite reductase small subunit (NCBI) compare
b3432 glgB +0.6 4.4 glycogen branching enzyme (NCBI) compare
b3082 ygjM +0.6 0.6 predicted DNA-binding transcriptional regulator (NCBI) compare
b0550 rusA +0.6 0.7 DLP12 prophage; endonuclease RUS (NCBI) compare
b0828 iaaA +0.6 1.3 L-asparaginase (NCBI) compare
b0696 kdpC +0.6 1.2 potassium-transporting ATPase subunit C (NCBI) compare
b2576 srmB +0.6 1.1 ATP-dependent RNA helicase (NCBI) compare
b1936 intG +0.6 0.5 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2123 yehR +0.6 1.1 orf, hypothetical protein (VIMSS) compare
b0864 artP +0.6 1.3 arginine transporter subunit (NCBI) compare
b1013 ycdC +0.6 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b1707 ydiV +0.6 1.8 hypothetical protein (NCBI) compare
b4260 pepA +0.6 3.3 leucyl aminopeptidase (NCBI) compare
b1615 uidC +0.6 1.8 membrane-associated protein (VIMSS) compare
b2328 mepA +0.6 3.1 penicillin-insensitive murein endopeptidase (NCBI) compare
b1423 ydcJ +0.6 1.1 hypothetical protein (NCBI) compare
b2537 hcaR +0.6 2.0 DNA-binding transcriptional activator of 3-phenylpropionic acid catabolism (NCBI) compare
b2187 yejL +0.6 1.0 hypothetical protein (NCBI) compare
b0065 yabI +0.6 2.3 conserved inner membrane protein (NCBI) compare
b2111 yehD +0.6 1.9 predicted fimbrial-like adhesin protein (NCBI) compare
b1591 b1591 +0.6 1.1 putative oxidoreductase component (VIMSS) compare
b3632 rfaQ +0.6 4.6 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0865 ybjP +0.6 1.9 predicted lipoprotein (NCBI) compare
b2539 hcaF +0.6 1.4 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI) compare
b1205 ychH +0.6 1.0 predicted inner membrane protein (NCBI) compare
b2221 atoD +0.6 1.0 acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI) compare
b4195 ulaC +0.6 1.3 L-ascorbate-specific enzyme IIA component of PTS (NCBI) compare
b2170 setB +0.6 1.9 lactose/glucose efflux system (NCBI) compare
b0920 ycbC +0.6 1.3 conserved inner membrane protein (NCBI) compare
b1153 ymfQ +0.6 1.0 e14 prophage; conserved protein (NCBI) compare
b2946 yggJ +0.6 1.6 orf, hypothetical protein (VIMSS) compare
b2951 yggS +0.6 1.4 predicted enzyme (NCBI) compare
b3494 yhiO +0.6 2.4 universal stress protein UspB (NCBI) compare
b1582 ynfA +0.6 1.6 hypothetical protein (NCBI) compare
b0597 ybdB +0.6 1.5 hypothetical protein (NCBI) compare
b0773 ybhB +0.6 2.9 predicted kinase inhibitor (NCBI) compare
b3644 yicC +0.6 2.4 hypothetical protein (NCBI) compare
b2811 ygdK +0.6 1.9 predicted Fe-S metabolism protein (NCBI) compare
b1884 cheR +0.5 2.1 chemotaxis regulator, protein-glutamate methyltransferase (NCBI) compare
b1155 ymfS +0.5 1.6 e14 prophage; predicted protein (NCBI) compare
b2317 dedA +0.5 2.0 conserved inner membrane protein (NCBI) compare
b0269 yagF +0.5 2.8 CP4-6 prophage; predicted dehydratase (NCBI) compare
b0329 yahO +0.5 1.9 hypothetical protein (NCBI) compare
b0995 torR +0.5 1.9 DNA-binding response regulator in two-component regulatory system with TorS (NCBI) compare
b2327 yfcA +0.5 1.7 conserved inner membrane protein (NCBI) compare
b0424 thiJ +0.5 1.8 4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis (VIMSS) compare
b2541 hcaB +0.5 2.3 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI) compare
b1519 tam +0.5 1.4 trans-aconitate 2-methyltransferase (NCBI) compare
b1755 ynjC +0.5 1.4 fused transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1156 tfaE +0.5 1.8 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b2288 nuoA +0.5 0.8 NADH dehydrogenase I chain A (VIMSS) compare
b0753 ybgS +0.5 1.5 hypothetical protein (NCBI) compare
b1812 pabB +0.5 1.7 para-aminobenzoate synthase component I (NCBI) compare
b2668 ygaP +0.5 2.2 predicted inner membrane protein with hydrolase activity (NCBI) compare
b1636 pdxY +0.5 1.4 pyridoxine kinase (NCBI) compare
b2064 asmA +0.5 2.4 predicted assembly protein (NCBI) compare
b3577 yiaM +0.5 2.3 predicted transporter (NCBI) compare
b1824 yobF +0.5 1.4 hypothetical protein (NCBI) compare
b4457 csrC +0.5 0.8 regulatory RNA (NCBI) compare
b1344 ydaO +0.5 0.9 predicted C32 tRNA thiolase (NCBI) compare
b2684 mprA +0.5 0.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1920 fliY +0.5 0.7 cystine transporter subunit (NCBI) compare


Specific Phenotypes

For 4 genes in this experiment

For carbon source D-Mannose in Escherichia coli BW25113

For carbon source D-Mannose across organisms